Klebsiella phage vB_Kpn_Chronis

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9MVE9|A0A5B9MVE9_9CAUD Putative conjugal transfer protein OS=Klebsiella phage vB_Kpn_Chronis OX=2591378 GN=CHRON_30 PE=4 SV=1
MM1 pKa = 7.7SDD3 pKa = 4.0LPVSYY8 pKa = 9.92TSAGPVPLTAEE19 pKa = 4.16EE20 pKa = 4.41LRR22 pKa = 11.84AQLVSQAIALSPGLTTDD39 pKa = 4.11LPGSLIEE46 pKa = 5.3DD47 pKa = 3.6VASTDD52 pKa = 3.14VGALIVCDD60 pKa = 3.6QARR63 pKa = 11.84VDD65 pKa = 5.4LINSVGPLKK74 pKa = 11.07ANLAMLEE81 pKa = 4.38LLAQQAGIPGQKK93 pKa = 8.44TAGTTTVPVQFSGPAGFVIPQGFIVSDD120 pKa = 3.52GTYY123 pKa = 9.19TYY125 pKa = 10.59SVSDD129 pKa = 3.34ATIISSSGVSASVSCEE145 pKa = 3.61GTEE148 pKa = 3.97TGTWAVPVNTVNQIISSLPSDD169 pKa = 3.66VTITCTNPIAGTPGADD185 pKa = 3.6PEE187 pKa = 4.52TNYY190 pKa = 10.29QFRR193 pKa = 11.84DD194 pKa = 3.97RR195 pKa = 11.84VWQAQMATVQGYY207 pKa = 8.99PGFIRR212 pKa = 11.84QYY214 pKa = 9.62LTSLDD219 pKa = 3.63NVQARR224 pKa = 11.84LVSVIQDD231 pKa = 3.21GDD233 pKa = 2.97KK234 pKa = 10.34WIVMCAGGDD243 pKa = 3.21IYY245 pKa = 11.02DD246 pKa = 3.88IAGALYY252 pKa = 10.24KK253 pKa = 10.58SAGDD257 pKa = 3.3ISRR260 pKa = 11.84LKK262 pKa = 10.58GCSLNVTGITNANPGVVSTDD282 pKa = 3.2LTHH285 pKa = 7.35GYY287 pKa = 8.81TDD289 pKa = 3.62GQVIRR294 pKa = 11.84ITGVTGMTGINDD306 pKa = 3.45VPLTVTVLSPHH317 pKa = 6.03TFSIGIDD324 pKa = 3.62TTSSGTWGGGGEE336 pKa = 4.11VTPNVRR342 pKa = 11.84NNTVTVNDD350 pKa = 3.6WPDD353 pKa = 2.96NYY355 pKa = 10.33VIPFVTPLLQRR366 pKa = 11.84VTVTYY371 pKa = 10.02QWGTEE376 pKa = 4.12SVNYY380 pKa = 8.89LTDD383 pKa = 3.2ATIASLVSAPTIQYY397 pKa = 10.5VNGIFAGKK405 pKa = 8.53PLNVNNLKK413 pKa = 10.63DD414 pKa = 3.74AFLQAINSTIDD425 pKa = 2.98MGLISTLNVVVTVNGVITPPDD446 pKa = 3.79AGTNIISGDD455 pKa = 3.5KK456 pKa = 10.4FSYY459 pKa = 10.1FYY461 pKa = 10.44IASDD465 pKa = 3.86GVIVTGAA472 pKa = 3.16

Molecular weight: 49.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9MSR3|A0A5B9MSR3_9CAUD Putative YlcG family protein OS=Klebsiella phage vB_Kpn_Chronis OX=2591378 GN=CHRON_64 PE=4 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84KK3 pKa = 8.26PARR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.32CAHH11 pKa = 5.64CRR13 pKa = 11.84EE14 pKa = 4.41WFHH17 pKa = 6.84PARR20 pKa = 11.84EE21 pKa = 4.26GQVVCSFEE29 pKa = 4.09CASAIGKK36 pKa = 8.72KK37 pKa = 7.2QTAKK41 pKa = 10.56ARR43 pKa = 11.84EE44 pKa = 3.79AAKK47 pKa = 10.37ARR49 pKa = 11.84EE50 pKa = 4.36VKK52 pKa = 10.06RR53 pKa = 11.84QRR55 pKa = 11.84EE56 pKa = 4.22SEE58 pKa = 4.0KK59 pKa = 10.31EE60 pKa = 3.66GRR62 pKa = 11.84QRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84AKK68 pKa = 10.5RR69 pKa = 11.84EE70 pKa = 3.81SFKK73 pKa = 10.93TKK75 pKa = 10.43AQWDD79 pKa = 4.06KK80 pKa = 10.73EE81 pKa = 4.19AQSAFNRR88 pKa = 11.84YY89 pKa = 8.22IRR91 pKa = 11.84IRR93 pKa = 11.84DD94 pKa = 3.38EE95 pKa = 4.24GKK97 pKa = 9.03PCVSCGSPLIGKK109 pKa = 9.58RR110 pKa = 11.84NYY112 pKa = 9.23LTGSAIDD119 pKa = 3.57ASHH122 pKa = 5.58YY123 pKa = 9.46RR124 pKa = 11.84SRR126 pKa = 11.84GAASHH131 pKa = 6.67LKK133 pKa = 9.12FNVFNVHH140 pKa = 6.17SACTRR145 pKa = 11.84CNRR148 pKa = 11.84QLSGNAVEE156 pKa = 4.08YY157 pKa = 10.25RR158 pKa = 11.84IRR160 pKa = 11.84LIEE163 pKa = 4.66RR164 pKa = 11.84IGLDD168 pKa = 2.76RR169 pKa = 11.84VEE171 pKa = 5.22RR172 pKa = 11.84LEE174 pKa = 3.99TDD176 pKa = 3.21NEE178 pKa = 3.83PRR180 pKa = 11.84RR181 pKa = 11.84FDD183 pKa = 3.05IPYY186 pKa = 8.71LQRR189 pKa = 11.84IKK191 pKa = 11.0SIFTRR196 pKa = 11.84RR197 pKa = 11.84ARR199 pKa = 11.84ALEE202 pKa = 3.86KK203 pKa = 10.4RR204 pKa = 11.84RR205 pKa = 11.84ARR207 pKa = 11.84HH208 pKa = 5.15QEE210 pKa = 3.52AAA212 pKa = 3.66

Molecular weight: 24.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

14677

31

849

206.7

22.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.13 ± 0.353

1.138 ± 0.121

6.03 ± 0.27

5.812 ± 0.405

3.509 ± 0.182

7.781 ± 0.4

1.492 ± 0.126

5.969 ± 0.221

5.246 ± 0.358

7.856 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.909 ± 0.134

4.756 ± 0.234

4.19 ± 0.196

4.183 ± 0.193

5.723 ± 0.346

6.82 ± 0.263

6.323 ± 0.403

6.343 ± 0.254

1.574 ± 0.118

3.216 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski