Amino acid dipepetide frequency for Streptomyces phage Tefunt

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.129AlaAla: 12.129 ± 1.167
0.513AlaCys: 0.513 ± 0.16
8.792AlaAsp: 8.792 ± 0.679
8.792AlaGlu: 8.792 ± 0.879
3.401AlaPhe: 3.401 ± 0.479
8.856AlaGly: 8.856 ± 0.849
2.054AlaHis: 2.054 ± 0.362
4.813AlaIle: 4.813 ± 0.585
6.033AlaLys: 6.033 ± 0.755
10.974AlaLeu: 10.974 ± 1.127
2.631AlaMet: 2.631 ± 0.366
2.952AlaAsn: 2.952 ± 0.449
4.685AlaPro: 4.685 ± 0.552
2.888AlaGln: 2.888 ± 0.593
7.059AlaArg: 7.059 ± 0.668
5.904AlaSer: 5.904 ± 0.695
6.225AlaThr: 6.225 ± 0.611
7.444AlaVal: 7.444 ± 0.684
2.631AlaTrp: 2.631 ± 0.359
3.594AlaTyr: 3.594 ± 0.527
0.0AlaXaa: 0.0 ± 0.0
Cys
0.834CysAla: 0.834 ± 0.254
0.064CysCys: 0.064 ± 0.07
0.513CysAsp: 0.513 ± 0.157
0.321CysGlu: 0.321 ± 0.138
0.128CysPhe: 0.128 ± 0.097
0.513CysGly: 0.513 ± 0.164
0.321CysHis: 0.321 ± 0.142
0.513CysIle: 0.513 ± 0.189
0.449CysLys: 0.449 ± 0.2
0.578CysLeu: 0.578 ± 0.221
0.064CysMet: 0.064 ± 0.058
0.257CysAsn: 0.257 ± 0.121
0.642CysPro: 0.642 ± 0.207
0.321CysGln: 0.321 ± 0.123
0.385CysArg: 0.385 ± 0.164
0.642CysSer: 0.642 ± 0.224
0.449CysThr: 0.449 ± 0.172
0.257CysVal: 0.257 ± 0.164
0.193CysTrp: 0.193 ± 0.111
0.193CysTyr: 0.193 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
7.444AspAla: 7.444 ± 0.602
0.513AspCys: 0.513 ± 0.173
4.236AspAsp: 4.236 ± 0.547
5.455AspGlu: 5.455 ± 0.649
1.54AspPhe: 1.54 ± 0.27
6.482AspGly: 6.482 ± 0.604
1.155AspHis: 1.155 ± 0.275
2.439AspIle: 2.439 ± 0.426
2.567AspLys: 2.567 ± 0.433
5.455AspLeu: 5.455 ± 0.673
1.476AspMet: 1.476 ± 0.271
1.284AspAsn: 1.284 ± 0.251
3.979AspPro: 3.979 ± 0.522
2.182AspGln: 2.182 ± 0.358
3.016AspArg: 3.016 ± 0.4
3.915AspSer: 3.915 ± 0.481
3.337AspThr: 3.337 ± 0.423
4.621AspVal: 4.621 ± 0.545
1.604AspTrp: 1.604 ± 0.295
1.284AspTyr: 1.284 ± 0.227
0.0AspXaa: 0.0 ± 0.0
Glu
7.83GluAla: 7.83 ± 0.865
0.834GluCys: 0.834 ± 0.24
4.107GluAsp: 4.107 ± 0.498
5.583GluGlu: 5.583 ± 0.812
2.503GluPhe: 2.503 ± 0.311
6.418GluGly: 6.418 ± 0.635
1.669GluHis: 1.669 ± 0.443
3.594GluIle: 3.594 ± 0.43
2.888GluLys: 2.888 ± 0.484
7.444GluLeu: 7.444 ± 0.752
0.963GluMet: 0.963 ± 0.259
1.669GluAsn: 1.669 ± 0.308
2.76GluPro: 2.76 ± 0.743
3.401GluGln: 3.401 ± 0.456
4.3GluArg: 4.3 ± 0.638
2.952GluSer: 2.952 ± 0.381
4.236GluThr: 4.236 ± 0.682
5.006GluVal: 5.006 ± 0.642
1.284GluTrp: 1.284 ± 0.282
2.631GluTyr: 2.631 ± 0.554
0.0GluXaa: 0.0 ± 0.0
Phe
3.722PheAla: 3.722 ± 0.613
0.128PheCys: 0.128 ± 0.111
2.182PheAsp: 2.182 ± 0.299
2.503PheGlu: 2.503 ± 0.338
0.706PhePhe: 0.706 ± 0.271
3.145PheGly: 3.145 ± 0.337
0.706PheHis: 0.706 ± 0.25
1.155PheIle: 1.155 ± 0.251
0.578PheLys: 0.578 ± 0.189
1.733PheLeu: 1.733 ± 0.421
1.027PheMet: 1.027 ± 0.309
1.219PheAsn: 1.219 ± 0.315
1.091PhePro: 1.091 ± 0.316
1.027PheGln: 1.027 ± 0.207
1.861PheArg: 1.861 ± 0.294
1.348PheSer: 1.348 ± 0.325
2.439PheThr: 2.439 ± 0.423
1.989PheVal: 1.989 ± 0.359
0.513PheTrp: 0.513 ± 0.178
0.834PheTyr: 0.834 ± 0.222
0.0PheXaa: 0.0 ± 0.0
Gly
8.279GlyAla: 8.279 ± 0.88
0.449GlyCys: 0.449 ± 0.17
4.749GlyAsp: 4.749 ± 0.67
5.391GlyGlu: 5.391 ± 0.593
3.016GlyPhe: 3.016 ± 0.426
6.225GlyGly: 6.225 ± 0.976
2.182GlyHis: 2.182 ± 0.399
3.401GlyIle: 3.401 ± 0.811
5.198GlyLys: 5.198 ± 0.612
6.803GlyLeu: 6.803 ± 0.817
1.219GlyMet: 1.219 ± 0.23
2.824GlyAsn: 2.824 ± 0.401
3.979GlyPro: 3.979 ± 0.55
3.466GlyGln: 3.466 ± 0.498
5.712GlyArg: 5.712 ± 0.891
5.198GlySer: 5.198 ± 0.716
5.198GlyThr: 5.198 ± 0.688
6.225GlyVal: 6.225 ± 0.568
1.925GlyTrp: 1.925 ± 0.394
2.375GlyTyr: 2.375 ± 0.378
0.0GlyXaa: 0.0 ± 0.0
His
1.861HisAla: 1.861 ± 0.328
0.128HisCys: 0.128 ± 0.09
1.027HisAsp: 1.027 ± 0.223
1.348HisGlu: 1.348 ± 0.34
1.027HisPhe: 1.027 ± 0.27
1.797HisGly: 1.797 ± 0.363
0.642HisHis: 0.642 ± 0.163
0.642HisIle: 0.642 ± 0.197
0.578HisLys: 0.578 ± 0.184
1.989HisLeu: 1.989 ± 0.38
0.257HisMet: 0.257 ± 0.127
0.385HisAsn: 0.385 ± 0.151
1.027HisPro: 1.027 ± 0.236
0.834HisGln: 0.834 ± 0.254
1.669HisArg: 1.669 ± 0.44
1.412HisSer: 1.412 ± 0.453
1.54HisThr: 1.54 ± 0.357
1.284HisVal: 1.284 ± 0.361
0.513HisTrp: 0.513 ± 0.167
0.706HisTyr: 0.706 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
4.685IleAla: 4.685 ± 0.548
0.321IleCys: 0.321 ± 0.136
3.658IleAsp: 3.658 ± 0.479
4.236IleGlu: 4.236 ± 0.489
1.412IlePhe: 1.412 ± 0.372
3.337IleGly: 3.337 ± 0.491
0.834IleHis: 0.834 ± 0.166
1.861IleIle: 1.861 ± 0.409
2.31IleLys: 2.31 ± 0.575
2.76IleLeu: 2.76 ± 0.51
0.449IleMet: 0.449 ± 0.19
1.476IleAsn: 1.476 ± 0.279
1.861IlePro: 1.861 ± 0.346
1.54IleGln: 1.54 ± 0.249
3.145IleArg: 3.145 ± 0.502
1.925IleSer: 1.925 ± 0.442
2.439IleThr: 2.439 ± 0.414
3.53IleVal: 3.53 ± 0.47
0.257IleTrp: 0.257 ± 0.126
1.027IleTyr: 1.027 ± 0.233
0.0IleXaa: 0.0 ± 0.0
Lys
4.364LysAla: 4.364 ± 0.79
0.385LysCys: 0.385 ± 0.138
2.695LysAsp: 2.695 ± 0.485
2.567LysGlu: 2.567 ± 0.409
1.027LysPhe: 1.027 ± 0.229
5.262LysGly: 5.262 ± 0.561
0.706LysHis: 0.706 ± 0.226
2.054LysIle: 2.054 ± 0.495
3.401LysLys: 3.401 ± 0.536
4.043LysLeu: 4.043 ± 0.599
0.963LysMet: 0.963 ± 0.25
1.027LysAsn: 1.027 ± 0.251
2.503LysPro: 2.503 ± 0.533
1.604LysGln: 1.604 ± 0.23
3.273LysArg: 3.273 ± 0.507
2.631LysSer: 2.631 ± 0.523
2.375LysThr: 2.375 ± 0.383
3.594LysVal: 3.594 ± 0.544
0.449LysTrp: 0.449 ± 0.21
1.54LysTyr: 1.54 ± 0.317
0.0LysXaa: 0.0 ± 0.0
Leu
11.423LeuAla: 11.423 ± 0.929
0.706LeuCys: 0.706 ± 0.238
4.877LeuAsp: 4.877 ± 0.612
4.428LeuGlu: 4.428 ± 0.561
1.669LeuPhe: 1.669 ± 0.418
7.38LeuGly: 7.38 ± 0.931
1.54LeuHis: 1.54 ± 0.292
3.53LeuIle: 3.53 ± 0.483
3.53LeuLys: 3.53 ± 0.68
6.225LeuLeu: 6.225 ± 0.763
1.733LeuMet: 1.733 ± 0.373
3.08LeuAsn: 3.08 ± 0.428
4.621LeuPro: 4.621 ± 0.6
2.567LeuGln: 2.567 ± 0.377
5.904LeuArg: 5.904 ± 0.755
5.904LeuSer: 5.904 ± 0.654
5.455LeuThr: 5.455 ± 0.529
5.391LeuVal: 5.391 ± 0.658
0.898LeuTrp: 0.898 ± 0.203
2.375LeuTyr: 2.375 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
3.979MetAla: 3.979 ± 0.492
0.193MetCys: 0.193 ± 0.098
1.412MetAsp: 1.412 ± 0.241
1.284MetGlu: 1.284 ± 0.318
0.513MetPhe: 0.513 ± 0.187
1.027MetGly: 1.027 ± 0.252
0.578MetHis: 0.578 ± 0.198
1.091MetIle: 1.091 ± 0.209
0.898MetLys: 0.898 ± 0.25
1.925MetLeu: 1.925 ± 0.342
0.257MetMet: 0.257 ± 0.128
0.321MetAsn: 0.321 ± 0.139
0.898MetPro: 0.898 ± 0.232
0.706MetGln: 0.706 ± 0.193
1.219MetArg: 1.219 ± 0.35
1.797MetSer: 1.797 ± 0.325
1.733MetThr: 1.733 ± 0.396
0.963MetVal: 0.963 ± 0.237
0.449MetTrp: 0.449 ± 0.164
0.385MetTyr: 0.385 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
3.016AsnAla: 3.016 ± 0.421
0.642AsnCys: 0.642 ± 0.216
1.284AsnAsp: 1.284 ± 0.262
2.375AsnGlu: 2.375 ± 0.356
0.963AsnPhe: 0.963 ± 0.278
2.695AsnGly: 2.695 ± 0.359
0.513AsnHis: 0.513 ± 0.179
1.155AsnIle: 1.155 ± 0.229
1.348AsnLys: 1.348 ± 0.277
2.567AsnLeu: 2.567 ± 0.406
0.513AsnMet: 0.513 ± 0.193
0.706AsnAsn: 0.706 ± 0.216
1.284AsnPro: 1.284 ± 0.281
0.834AsnGln: 0.834 ± 0.2
1.476AsnArg: 1.476 ± 0.241
1.476AsnSer: 1.476 ± 0.331
1.669AsnThr: 1.669 ± 0.352
1.604AsnVal: 1.604 ± 0.28
0.513AsnTrp: 0.513 ± 0.17
0.834AsnTyr: 0.834 ± 0.162
0.0AsnXaa: 0.0 ± 0.0
Pro
5.391ProAla: 5.391 ± 0.681
0.449ProCys: 0.449 ± 0.159
3.594ProAsp: 3.594 ± 0.403
4.043ProGlu: 4.043 ± 0.722
0.963ProPhe: 0.963 ± 0.227
3.594ProGly: 3.594 ± 0.412
0.385ProHis: 0.385 ± 0.143
2.118ProIle: 2.118 ± 0.36
2.952ProLys: 2.952 ± 0.448
3.273ProLeu: 3.273 ± 0.496
1.284ProMet: 1.284 ± 0.245
0.963ProAsn: 0.963 ± 0.215
2.31ProPro: 2.31 ± 0.48
1.476ProGln: 1.476 ± 0.263
3.466ProArg: 3.466 ± 0.521
2.888ProSer: 2.888 ± 0.576
2.824ProThr: 2.824 ± 0.435
3.594ProVal: 3.594 ± 0.551
0.898ProTrp: 0.898 ± 0.22
1.219ProTyr: 1.219 ± 0.348
0.0ProXaa: 0.0 ± 0.0
Gln
4.685GlnAla: 4.685 ± 0.499
0.193GlnCys: 0.193 ± 0.1
1.733GlnAsp: 1.733 ± 0.352
2.439GlnGlu: 2.439 ± 0.514
1.091GlnPhe: 1.091 ± 0.248
2.054GlnGly: 2.054 ± 0.337
0.449GlnHis: 0.449 ± 0.156
1.925GlnIle: 1.925 ± 0.252
1.476GlnLys: 1.476 ± 0.349
3.273GlnLeu: 3.273 ± 0.721
0.963GlnMet: 0.963 ± 0.278
0.642GlnAsn: 0.642 ± 0.154
1.861GlnPro: 1.861 ± 0.355
1.155GlnGln: 1.155 ± 0.278
2.824GlnArg: 2.824 ± 0.394
2.439GlnSer: 2.439 ± 0.333
1.54GlnThr: 1.54 ± 0.357
2.695GlnVal: 2.695 ± 0.455
0.578GlnTrp: 0.578 ± 0.255
0.834GlnTyr: 0.834 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
6.482ArgAla: 6.482 ± 0.895
0.706ArgCys: 0.706 ± 0.233
3.979ArgAsp: 3.979 ± 0.441
5.07ArgGlu: 5.07 ± 0.806
2.246ArgPhe: 2.246 ± 0.41
4.749ArgGly: 4.749 ± 0.742
1.54ArgHis: 1.54 ± 0.355
3.016ArgIle: 3.016 ± 0.377
3.273ArgLys: 3.273 ± 0.469
5.391ArgLeu: 5.391 ± 0.673
1.925ArgMet: 1.925 ± 0.403
2.118ArgAsn: 2.118 ± 0.326
2.952ArgPro: 2.952 ± 0.504
2.824ArgGln: 2.824 ± 0.523
4.685ArgArg: 4.685 ± 0.753
3.722ArgSer: 3.722 ± 0.483
3.786ArgThr: 3.786 ± 0.558
4.813ArgVal: 4.813 ± 0.605
0.77ArgTrp: 0.77 ± 0.233
1.733ArgTyr: 1.733 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
6.161SerAla: 6.161 ± 0.746
0.193SerCys: 0.193 ± 0.089
3.209SerAsp: 3.209 ± 0.395
3.979SerGlu: 3.979 ± 0.403
1.925SerPhe: 1.925 ± 0.323
5.776SerGly: 5.776 ± 0.7
1.604SerHis: 1.604 ± 0.563
2.952SerIle: 2.952 ± 0.376
2.439SerLys: 2.439 ± 0.364
4.942SerLeu: 4.942 ± 0.643
1.284SerMet: 1.284 ± 0.271
1.284SerAsn: 1.284 ± 0.251
2.054SerPro: 2.054 ± 0.316
2.182SerGln: 2.182 ± 0.34
4.236SerArg: 4.236 ± 0.547
4.107SerSer: 4.107 ± 0.773
4.428SerThr: 4.428 ± 0.789
4.236SerVal: 4.236 ± 0.443
0.963SerTrp: 0.963 ± 0.289
1.861SerTyr: 1.861 ± 0.295
0.0SerXaa: 0.0 ± 0.0
Thr
7.38ThrAla: 7.38 ± 0.643
0.449ThrCys: 0.449 ± 0.214
4.043ThrAsp: 4.043 ± 0.524
3.401ThrGlu: 3.401 ± 0.528
2.375ThrPhe: 2.375 ± 0.473
5.455ThrGly: 5.455 ± 0.802
1.219ThrHis: 1.219 ± 0.291
2.503ThrIle: 2.503 ± 0.453
1.54ThrLys: 1.54 ± 0.294
4.364ThrLeu: 4.364 ± 0.491
1.476ThrMet: 1.476 ± 0.344
1.54ThrAsn: 1.54 ± 0.399
3.786ThrPro: 3.786 ± 0.712
2.054ThrGln: 2.054 ± 0.362
3.273ThrArg: 3.273 ± 0.398
4.877ThrSer: 4.877 ± 0.708
3.722ThrThr: 3.722 ± 0.526
6.033ThrVal: 6.033 ± 0.657
0.963ThrTrp: 0.963 ± 0.221
2.246ThrTyr: 2.246 ± 0.37
0.0ThrXaa: 0.0 ± 0.0
Val
7.701ValAla: 7.701 ± 0.666
0.449ValCys: 0.449 ± 0.156
4.171ValAsp: 4.171 ± 0.394
5.455ValGlu: 5.455 ± 0.682
2.118ValPhe: 2.118 ± 0.31
5.262ValGly: 5.262 ± 0.61
1.412ValHis: 1.412 ± 0.307
3.016ValIle: 3.016 ± 0.425
3.337ValLys: 3.337 ± 0.532
5.968ValLeu: 5.968 ± 0.781
1.797ValMet: 1.797 ± 0.27
2.31ValAsn: 2.31 ± 0.371
3.594ValPro: 3.594 ± 0.504
2.76ValGln: 2.76 ± 0.441
4.749ValArg: 4.749 ± 0.557
3.594ValSer: 3.594 ± 0.496
6.097ValThr: 6.097 ± 0.778
4.749ValVal: 4.749 ± 0.578
1.412ValTrp: 1.412 ± 0.263
1.155ValTyr: 1.155 ± 0.304
0.0ValXaa: 0.0 ± 0.0
Trp
2.246TrpAla: 2.246 ± 0.422
0.193TrpCys: 0.193 ± 0.106
1.348TrpAsp: 1.348 ± 0.28
1.091TrpGlu: 1.091 ± 0.263
0.449TrpPhe: 0.449 ± 0.166
1.284TrpGly: 1.284 ± 0.279
0.513TrpHis: 0.513 ± 0.181
0.257TrpIle: 0.257 ± 0.119
0.898TrpLys: 0.898 ± 0.195
1.348TrpLeu: 1.348 ± 0.309
0.642TrpMet: 0.642 ± 0.189
0.578TrpAsn: 0.578 ± 0.183
0.642TrpPro: 0.642 ± 0.191
0.321TrpGln: 0.321 ± 0.153
1.348TrpArg: 1.348 ± 0.223
1.284TrpSer: 1.284 ± 0.253
1.604TrpThr: 1.604 ± 0.298
1.155TrpVal: 1.155 ± 0.325
0.193TrpTrp: 0.193 ± 0.118
0.257TrpTyr: 0.257 ± 0.121
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.76TyrAla: 2.76 ± 0.357
0.128TyrCys: 0.128 ± 0.105
2.375TyrAsp: 2.375 ± 0.453
2.31TyrGlu: 2.31 ± 0.412
0.834TyrPhe: 0.834 ± 0.227
2.503TyrGly: 2.503 ± 0.515
0.642TyrHis: 0.642 ± 0.306
1.027TyrIle: 1.027 ± 0.266
0.77TyrLys: 0.77 ± 0.228
2.182TyrLeu: 2.182 ± 0.452
0.642TyrMet: 0.642 ± 0.185
0.834TyrAsn: 0.834 ± 0.229
1.155TyrPro: 1.155 ± 0.315
0.706TyrGln: 0.706 ± 0.2
2.182TyrArg: 2.182 ± 0.402
1.861TyrSer: 1.861 ± 0.341
1.604TyrThr: 1.604 ± 0.297
1.925TyrVal: 1.925 ± 0.349
0.642TyrTrp: 0.642 ± 0.206
1.027TyrTyr: 1.027 ± 0.258
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (15583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski