Dinoroseobacter phage vB_DshS-R4C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cronusvirus; unclassified Cronusvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EGT0|A0A4Y6EGT0_9CAUD Transcriptional regulator OS=Dinoroseobacter phage vB_DshS-R4C OX=2590919 GN=vBDshSR4C_020 PE=4 SV=1
MM1 pKa = 7.63LKK3 pKa = 10.76GKK5 pKa = 10.45DD6 pKa = 3.51GVVKK10 pKa = 10.56NASTGDD16 pKa = 4.16SIGHH20 pKa = 5.29LQSWALDD27 pKa = 3.69TQRR30 pKa = 11.84DD31 pKa = 4.0EE32 pKa = 4.23VSGWGMGDD40 pKa = 3.24DD41 pKa = 4.16AEE43 pKa = 4.56RR44 pKa = 11.84AFTTVGRR51 pKa = 11.84ASGNFEE57 pKa = 4.44VYY59 pKa = 10.52LDD61 pKa = 4.23PADD64 pKa = 4.56PSDD67 pKa = 4.12DD68 pKa = 4.66LEE70 pKa = 6.16PGDD73 pKa = 5.41LVDD76 pKa = 5.53LEE78 pKa = 5.08LYY80 pKa = 10.2PGGEE84 pKa = 4.51STGSGYY90 pKa = 10.65RR91 pKa = 11.84SVAGALILSTAEE103 pKa = 4.06SASKK107 pKa = 10.72DD108 pKa = 4.18GIPMLTVNWRR118 pKa = 11.84TSGALPQKK126 pKa = 10.03ATVSS130 pKa = 3.55

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6E802|A0A4Y6E802_9CAUD DNA primase OS=Dinoroseobacter phage vB_DshS-R4C OX=2590919 GN=vBDshSR4C_043 PE=4 SV=1
MM1 pKa = 7.65ASASPRR7 pKa = 11.84TASTSRR13 pKa = 11.84SSLKK17 pKa = 9.41NTGKK21 pKa = 10.37YY22 pKa = 9.78FGPSRR27 pKa = 11.84VTSCAAMLKK36 pKa = 10.05ARR38 pKa = 11.84SSRR41 pKa = 11.84TRR43 pKa = 11.84SPDD46 pKa = 3.58TISHH50 pKa = 6.5LEE52 pKa = 3.88VEE54 pKa = 4.81RR55 pKa = 11.84GQLAGKK61 pKa = 9.85CLVSHH66 pKa = 6.66PRR68 pKa = 11.84RR69 pKa = 11.84LRR71 pKa = 11.84GLGLVPPGAIQYY83 pKa = 8.28PADD86 pKa = 3.42IFPFKK91 pKa = 10.15IAQHH95 pKa = 6.04MGQGAPGHH103 pKa = 5.73IKK105 pKa = 10.68GYY107 pKa = 8.05GLRR110 pKa = 11.84PGALYY115 pKa = 10.91GLVGHH120 pKa = 6.28VLLPRR125 pKa = 11.84RR126 pKa = 11.84VCVFCSPHH134 pKa = 5.97SCAGQHH140 pKa = 5.87

Molecular weight: 14.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11636

36

1447

237.5

25.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.524 ± 0.499

0.971 ± 0.187

6.617 ± 0.38

4.924 ± 0.247

3.068 ± 0.194

9.582 ± 0.428

1.77 ± 0.217

4.022 ± 0.171

2.621 ± 0.275

8.912 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.813 ± 0.136

1.813 ± 0.159

6.652 ± 0.467

2.277 ± 0.161

8.19 ± 0.373

5.277 ± 0.294

6.557 ± 0.297

6.772 ± 0.328

1.788 ± 0.143

1.848 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski