Treponema sp. JC4

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; unclassified Treponema

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0XC94|I0XC94_9SPIO DNA topoisomerase (ATP-hydrolyzing) OS=Treponema sp. JC4 OX=1124982 GN=MSI_02160 PE=4 SV=1
MM1 pKa = 7.68EE2 pKa = 5.01SFSIIEE8 pKa = 4.2SEE10 pKa = 4.25TANVTFSGDD19 pKa = 3.59DD20 pKa = 3.5SDD22 pKa = 5.42LKK24 pKa = 10.37TVLAASTSTSYY35 pKa = 8.5QTALSTFFTTSKK47 pKa = 10.48YY48 pKa = 10.72YY49 pKa = 10.98KK50 pKa = 9.63NDD52 pKa = 3.2EE53 pKa = 4.69NIVDD57 pKa = 3.99TNEE60 pKa = 3.58TYY62 pKa = 10.63YY63 pKa = 11.1AQLGIPCGDD72 pKa = 3.34

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0XC96|I0XC96_9SPIO Uncharacterized protein OS=Treponema sp. JC4 OX=1124982 GN=MSI_02180 PE=4 SV=1
MM1 pKa = 7.48LSDD4 pKa = 3.86ASRR7 pKa = 11.84GKK9 pKa = 10.4DD10 pKa = 3.14EE11 pKa = 4.91NGPNGRR17 pKa = 11.84KK18 pKa = 7.76ITKK21 pKa = 8.39LTYY24 pKa = 9.66NEE26 pKa = 3.75CRR28 pKa = 11.84KK29 pKa = 9.4IRR31 pKa = 11.84KK32 pKa = 7.33TRR34 pKa = 11.84GLPDD38 pKa = 2.97INVRR42 pKa = 11.84FIEE45 pKa = 4.05HH46 pKa = 7.02RR47 pKa = 11.84EE48 pKa = 3.75NMGLVEE54 pKa = 4.31VRR56 pKa = 11.84CPSHH60 pKa = 7.16AMRR63 pKa = 11.84LLGRR67 pKa = 11.84RR68 pKa = 11.84LRR70 pKa = 11.84YY71 pKa = 9.08PNRR74 pKa = 11.84KK75 pKa = 8.99IYY77 pKa = 10.4EE78 pKa = 4.14SS79 pKa = 3.52

Molecular weight: 9.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2570

0

2570

882495

32

4046

343.4

38.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.72 ± 0.054

1.394 ± 0.021

5.699 ± 0.039

7.031 ± 0.056

4.908 ± 0.04

6.598 ± 0.045

1.531 ± 0.018

7.455 ± 0.04

7.613 ± 0.044

9.002 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.43 ± 0.023

5.051 ± 0.028

3.42 ± 0.029

3.106 ± 0.026

3.748 ± 0.032

6.769 ± 0.059

5.474 ± 0.047

6.256 ± 0.04

0.959 ± 0.017

3.838 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski