Achromobacter phage vB_AxyP_19-32_Axy13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Rothmandenesvirinae; Jwalphavirus; unclassified Jwalphavirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CUQ6|A0A514CUQ6_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy13 OX=2591044 GN=Axy13_070 PE=4 SV=1
MM1 pKa = 7.54SLFTYY6 pKa = 10.34PEE8 pKa = 4.12EE9 pKa = 4.2EE10 pKa = 4.05HH11 pKa = 6.7NSRR14 pKa = 11.84VMCGDD19 pKa = 4.06PVALAYY25 pKa = 9.19EE26 pKa = 4.2WLKK29 pKa = 11.07QYY31 pKa = 11.28AEE33 pKa = 4.36EE34 pKa = 4.35VNNDD38 pKa = 3.32AGDD41 pKa = 3.75EE42 pKa = 4.5GEE44 pKa = 4.42VTLEE48 pKa = 4.39DD49 pKa = 4.03LLEE52 pKa = 4.51AADD55 pKa = 3.81SHH57 pKa = 7.72QGDD60 pKa = 3.04GWGDD64 pKa = 3.56YY65 pKa = 9.87IVRR68 pKa = 11.84GGAFEE73 pKa = 5.27GYY75 pKa = 9.88SLNPMFWDD83 pKa = 4.18KK84 pKa = 11.39YY85 pKa = 10.92AIFKK89 pKa = 11.02GIDD92 pKa = 3.02RR93 pKa = 11.84SEE95 pKa = 3.71ISEE98 pKa = 3.96AHH100 pKa = 6.37FFSCSCC106 pKa = 4.2

Molecular weight: 11.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CUS2|A0A514CUS2_9CAUD Uncharacterized protein OS=Achromobacter phage vB_AxyP_19-32_Axy13 OX=2591044 GN=Axy13_031 PE=4 SV=1
MM1 pKa = 7.66LNYY4 pKa = 10.6NPFAFVLGVAIAVAVLLLCEE24 pKa = 4.18PLMWALIYY32 pKa = 9.92LAKK35 pKa = 10.72ALIVLVAVLMPTALTLEE52 pKa = 3.97WWHH55 pKa = 5.89NRR57 pKa = 11.84RR58 pKa = 11.84TKK60 pKa = 10.56KK61 pKa = 10.11EE62 pKa = 4.02KK63 pKa = 10.18QDD65 pKa = 3.39AQAPTFTIRR74 pKa = 3.73

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

21568

41

3471

303.8

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.718 ± 0.618

0.663 ± 0.113

6.273 ± 0.132

6.306 ± 0.223

3.343 ± 0.177

7.349 ± 0.252

1.887 ± 0.176

5.077 ± 0.239

6.213 ± 0.213

8.16 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.222 ± 0.128

4.683 ± 0.178

4.47 ± 0.234

4.817 ± 0.206

4.757 ± 0.192

5.439 ± 0.168

5.888 ± 0.217

6.839 ± 0.196

1.53 ± 0.215

3.361 ± 0.233
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski