Amino acid dipepetide frequency for Streptomyces griseoflavus Tu4000

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.838AlaAla: 19.838 ± 0.148	1.037AlaCys: 1.037 ± 0.02	8.064AlaAsp: 8.064 ± 0.062	8.636AlaGlu: 8.636 ± 0.094	3.422AlaPhe: 3.422 ± 0.045	13.409AlaGly: 13.409 ± 0.099	2.935AlaHis: 2.935 ± 0.038	3.145AlaIle: 3.145 ± 0.044	2.731AlaLys: 2.731 ± 0.049	14.053AlaLeu: 14.053 ± 0.114	2.485AlaMet: 2.485 ± 0.036	1.866AlaAsn: 1.866 ± 0.036	6.849AlaPro: 6.849 ± 0.069	3.456AlaGln: 3.456 ± 0.048	10.581AlaArg: 10.581 ± 0.089	5.706AlaSer: 5.706 ± 0.056	6.481AlaThr: 6.481 ± 0.058	12.161AlaVal: 12.161 ± 0.092	1.789AlaTrp: 1.789 ± 0.034	2.719AlaTyr: 2.719 ± 0.036	0.0AlaXaa: 0.0 ± 0.0
Cys	1.103CysAla: 1.103 ± 0.024	0.111CysCys: 0.111 ± 0.008	0.486CysAsp: 0.486 ± 0.018	0.409CysGlu: 0.409 ± 0.014	0.222CysPhe: 0.222 ± 0.012	0.992CysGly: 0.992 ± 0.025	0.216CysHis: 0.216 ± 0.011	0.151CysIle: 0.151 ± 0.009	0.107CysLys: 0.107 ± 0.007	0.74CysLeu: 0.74 ± 0.02	0.127CysMet: 0.127 ± 0.008	0.134CysAsn: 0.134 ± 0.008	0.514CysPro: 0.514 ± 0.018	0.162CysGln: 0.162 ± 0.008	0.67CysArg: 0.67 ± 0.017	0.466CysSer: 0.466 ± 0.016	0.523CysThr: 0.523 ± 0.017	0.673CysVal: 0.673 ± 0.015	0.137CysTrp: 0.137 ± 0.008	0.157CysTyr: 0.157 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.675AspAla: 7.675 ± 0.07	0.434AspCys: 0.434 ± 0.013	3.794AspAsp: 3.794 ± 0.051	4.024AspGlu: 4.024 ± 0.053	1.729AspPhe: 1.729 ± 0.03	6.624AspGly: 6.624 ± 0.07	1.434AspHis: 1.434 ± 0.028	1.897AspIle: 1.897 ± 0.032	1.209AspLys: 1.209 ± 0.028	6.107AspLeu: 6.107 ± 0.055	0.895AspMet: 0.895 ± 0.021	1.009AspAsn: 1.009 ± 0.024	4.483AspPro: 4.483 ± 0.048	1.439AspGln: 1.439 ± 0.026	5.06AspArg: 5.06 ± 0.052	2.608AspSer: 2.608 ± 0.038	3.282AspThr: 3.282 ± 0.043	4.811AspVal: 4.811 ± 0.046	1.057AspTrp: 1.057 ± 0.028	1.148AspTyr: 1.148 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.326GluAla: 7.326 ± 0.081	0.372GluCys: 0.372 ± 0.014	3.032GluAsp: 3.032 ± 0.042	3.843GluGlu: 3.843 ± 0.058	1.495GluPhe: 1.495 ± 0.029	4.545GluGly: 4.545 ± 0.057	1.539GluHis: 1.539 ± 0.029	2.308GluIle: 2.308 ± 0.036	1.609GluLys: 1.609 ± 0.038	6.727GluLeu: 6.727 ± 0.077	0.942GluMet: 0.942 ± 0.024	1.125GluAsn: 1.125 ± 0.029	3.46GluPro: 3.46 ± 0.056	2.236GluGln: 2.236 ± 0.044	5.756GluArg: 5.756 ± 0.069	2.661GluSer: 2.661 ± 0.035	2.99GluThr: 2.99 ± 0.037	4.574GluVal: 4.574 ± 0.054	0.807GluTrp: 0.807 ± 0.017	1.199GluTyr: 1.199 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.611PheAla: 3.611 ± 0.045	0.269PheCys: 0.269 ± 0.011	1.961PheAsp: 1.961 ± 0.035	1.459PheGlu: 1.459 ± 0.032	1.002PhePhe: 1.002 ± 0.034	3.056PheGly: 3.056 ± 0.04	0.62PheHis: 0.62 ± 0.019	0.673PheIle: 0.673 ± 0.016	0.515PheLys: 0.515 ± 0.018	2.668PheLeu: 2.668 ± 0.041	0.46PheMet: 0.46 ± 0.016	0.576PheAsn: 0.576 ± 0.018	1.392PhePro: 1.392 ± 0.03	0.661PheGln: 0.661 ± 0.019	1.948PheArg: 1.948 ± 0.036	1.446PheSer: 1.446 ± 0.026	2.033PheThr: 2.033 ± 0.036	2.315PheVal: 2.315 ± 0.039	0.425PheTrp: 0.425 ± 0.015	0.591PheTyr: 0.591 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	11.033GlyAla: 11.033 ± 0.09	0.878GlyCys: 0.878 ± 0.022	5.513GlyAsp: 5.513 ± 0.056	5.434GlyGlu: 5.434 ± 0.055	2.896GlyPhe: 2.896 ± 0.039	9.191GlyGly: 9.191 ± 0.106	2.42GlyHis: 2.42 ± 0.035	3.364GlyIle: 3.364 ± 0.045	2.431GlyLys: 2.431 ± 0.046	9.374GlyLeu: 9.374 ± 0.075	2.063GlyMet: 2.063 ± 0.029	1.819GlyAsn: 1.819 ± 0.037	5.265GlyPro: 5.265 ± 0.06	2.606GlyGln: 2.606 ± 0.035	8.535GlyArg: 8.535 ± 0.079	5.377GlySer: 5.377 ± 0.071	6.396GlyThr: 6.396 ± 0.07	7.696GlyVal: 7.696 ± 0.069	1.693GlyTrp: 1.693 ± 0.029	2.247GlyTyr: 2.247 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.686HisAla: 2.686 ± 0.043	0.216HisCys: 0.216 ± 0.011	1.367HisAsp: 1.367 ± 0.029	1.218HisGlu: 1.218 ± 0.026	0.662HisPhe: 0.662 ± 0.015	2.528HisGly: 2.528 ± 0.043	0.725HisHis: 0.725 ± 0.02	0.686HisIle: 0.686 ± 0.017	0.361HisLys: 0.361 ± 0.012	2.413HisLeu: 2.413 ± 0.035	0.366HisMet: 0.366 ± 0.013	0.368HisAsn: 0.368 ± 0.013	1.877HisPro: 1.877 ± 0.032	0.64HisGln: 0.64 ± 0.018	2.3HisArg: 2.3 ± 0.045	1.006HisSer: 1.006 ± 0.023	1.374HisThr: 1.374 ± 0.027	1.714HisVal: 1.714 ± 0.031	0.38HisTrp: 0.38 ± 0.013	0.504HisTyr: 0.504 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.439IleAla: 4.439 ± 0.058	0.277IleCys: 0.277 ± 0.011	2.16IleAsp: 2.16 ± 0.033	1.931IleGlu: 1.931 ± 0.036	0.72IlePhe: 0.72 ± 0.018	3.442IleGly: 3.442 ± 0.042	0.583IleHis: 0.583 ± 0.017	0.795IleIle: 0.795 ± 0.023	0.709IleLys: 0.709 ± 0.022	2.334IleLeu: 2.334 ± 0.038	0.466IleMet: 0.466 ± 0.018	0.673IleAsn: 0.673 ± 0.02	1.667IlePro: 1.667 ± 0.03	0.674IleGln: 0.674 ± 0.018	2.234IleArg: 2.234 ± 0.033	1.552IleSer: 1.552 ± 0.029	2.14IleThr: 2.14 ± 0.031	2.632IleVal: 2.632 ± 0.041	0.336IleTrp: 0.336 ± 0.013	0.5IleTyr: 0.5 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.851LysAla: 2.851 ± 0.051	0.118LysCys: 0.118 ± 0.008	1.38LysAsp: 1.38 ± 0.033	1.294LysGlu: 1.294 ± 0.027	0.462LysPhe: 0.462 ± 0.016	1.888LysGly: 1.888 ± 0.036	0.427LysHis: 0.427 ± 0.015	0.816LysIle: 0.816 ± 0.022	0.949LysLys: 0.949 ± 0.034	1.98LysLeu: 1.98 ± 0.035	0.379LysMet: 0.379 ± 0.013	0.546LysAsn: 0.546 ± 0.018	1.291LysPro: 1.291 ± 0.032	0.712LysGln: 0.712 ± 0.018	1.55LysArg: 1.55 ± 0.032	1.152LysSer: 1.152 ± 0.029	1.286LysThr: 1.286 ± 0.034	1.871LysVal: 1.871 ± 0.031	0.283LysTrp: 0.283 ± 0.012	0.491LysTyr: 0.491 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	14.279LeuAla: 14.279 ± 0.105	0.84LeuCys: 0.84 ± 0.021	6.712LeuAsp: 6.712 ± 0.058	4.927LeuGlu: 4.927 ± 0.058	2.644LeuPhe: 2.644 ± 0.043	9.152LeuGly: 9.152 ± 0.079	2.284LeuHis: 2.284 ± 0.035	3.129LeuIle: 3.129 ± 0.043	2.047LeuLys: 2.047 ± 0.038	11.045LeuLeu: 11.045 ± 0.11	1.67LeuMet: 1.67 ± 0.032	1.707LeuAsn: 1.707 ± 0.031	6.436LeuPro: 6.436 ± 0.057	2.147LeuGln: 2.147 ± 0.036	8.811LeuArg: 8.811 ± 0.094	5.258LeuSer: 5.258 ± 0.049	6.893LeuThr: 6.893 ± 0.065	8.691LeuVal: 8.691 ± 0.081	1.301LeuTrp: 1.301 ± 0.028	1.889LeuTyr: 1.889 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.268MetAla: 2.268 ± 0.04	0.152MetCys: 0.152 ± 0.009	0.936MetAsp: 0.936 ± 0.02	0.819MetGlu: 0.819 ± 0.021	0.499MetPhe: 0.499 ± 0.018	1.376MetGly: 1.376 ± 0.029	0.368MetHis: 0.368 ± 0.013	0.667MetIle: 0.667 ± 0.022	0.42MetLys: 0.42 ± 0.013	1.717MetLeu: 1.717 ± 0.027	0.337MetMet: 0.337 ± 0.014	0.449MetAsn: 0.449 ± 0.014	1.168MetPro: 1.168 ± 0.025	0.45MetGln: 0.45 ± 0.014	1.545MetArg: 1.545 ± 0.027	1.353MetSer: 1.353 ± 0.026	1.571MetThr: 1.571 ± 0.028	1.307MetVal: 1.307 ± 0.026	0.229MetTrp: 0.229 ± 0.012	0.334MetTyr: 0.334 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.169AsnAla: 2.169 ± 0.035	0.181AsnCys: 0.181 ± 0.01	0.979AsnAsp: 0.979 ± 0.023	0.855AsnGlu: 0.855 ± 0.021	0.506AsnPhe: 0.506 ± 0.014	1.908AsnGly: 1.908 ± 0.033	0.414AsnHis: 0.414 ± 0.015	0.638AsnIle: 0.638 ± 0.017	0.432AsnLys: 0.432 ± 0.014	1.659AsnLeu: 1.659 ± 0.027	0.318AsnMet: 0.318 ± 0.013	0.438AsnAsn: 0.438 ± 0.019	1.35AsnPro: 1.35 ± 0.029	0.518AsnGln: 0.518 ± 0.019	1.342AsnArg: 1.342 ± 0.026	0.919AsnSer: 0.919 ± 0.024	1.11AsnThr: 1.11 ± 0.028	1.423AsnVal: 1.423 ± 0.03	0.31AsnTrp: 0.31 ± 0.014	0.43AsnTyr: 0.43 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	8.568ProAla: 8.568 ± 0.079	0.373ProCys: 0.373 ± 0.015	4.508ProAsp: 4.508 ± 0.048	4.488ProGlu: 4.488 ± 0.047	1.569ProPhe: 1.569 ± 0.026	6.892ProGly: 6.892 ± 0.063	1.441ProHis: 1.441 ± 0.026	1.129ProIle: 1.129 ± 0.025	1.137ProLys: 1.137 ± 0.028	5.338ProLeu: 5.338 ± 0.055	1.053ProMet: 1.053 ± 0.025	0.851ProAsn: 0.851 ± 0.024	3.559ProPro: 3.559 ± 0.054	1.534ProGln: 1.534 ± 0.032	4.429ProArg: 4.429 ± 0.051	3.222ProSer: 3.222 ± 0.046	2.929ProThr: 2.929 ± 0.04	5.506ProVal: 5.506 ± 0.05	0.869ProTrp: 0.869 ± 0.023	1.436ProTyr: 1.436 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.42GlnAla: 3.42 ± 0.05	0.167GlnCys: 0.167 ± 0.009	1.402GlnAsp: 1.402 ± 0.026	1.463GlnGlu: 1.463 ± 0.032	0.657GlnPhe: 0.657 ± 0.019	2.228GlnGly: 2.228 ± 0.035	0.657GlnHis: 0.657 ± 0.019	1.028GlnIle: 1.028 ± 0.023	0.652GlnLys: 0.652 ± 0.021	2.82GlnLeu: 2.82 ± 0.04	0.498GlnMet: 0.498 ± 0.015	0.518GlnAsn: 0.518 ± 0.015	1.582GlnPro: 1.582 ± 0.035	1.179GlnGln: 1.179 ± 0.029	2.333GlnArg: 2.333 ± 0.037	1.235GlnSer: 1.235 ± 0.027	1.264GlnThr: 1.264 ± 0.026	2.261GlnVal: 2.261 ± 0.041	0.439GlnTrp: 0.439 ± 0.016	0.629GlnTyr: 0.629 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	10.149ArgAla: 10.149 ± 0.102	0.663ArgCys: 0.663 ± 0.019	4.567ArgAsp: 4.567 ± 0.053	5.106ArgGlu: 5.106 ± 0.058	2.412ArgPhe: 2.412 ± 0.037	6.398ArgGly: 6.398 ± 0.061	2.346ArgHis: 2.346 ± 0.043	3.144ArgIle: 3.144 ± 0.042	1.684ArgLys: 1.684 ± 0.029	9.063ArgLeu: 9.063 ± 0.093	1.86ArgMet: 1.86 ± 0.031	1.436ArgAsn: 1.436 ± 0.028	5.449ArgPro: 5.449 ± 0.071	2.367ArgGln: 2.367 ± 0.037	8.651ArgArg: 8.651 ± 0.109	4.193ArgSer: 4.193 ± 0.042	5.617ArgThr: 5.617 ± 0.049	6.218ArgVal: 6.218 ± 0.066	1.424ArgTrp: 1.424 ± 0.03	1.836ArgTyr: 1.836 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.523SerAla: 6.523 ± 0.063	0.435SerCys: 0.435 ± 0.013	2.681SerAsp: 2.681 ± 0.035	2.466SerGlu: 2.466 ± 0.04	1.512SerPhe: 1.512 ± 0.028	5.956SerGly: 5.956 ± 0.071	1.024SerHis: 1.024 ± 0.022	1.304SerIle: 1.304 ± 0.028	1.012SerLys: 1.012 ± 0.022	4.812SerLeu: 4.812 ± 0.052	1.03SerMet: 1.03 ± 0.025	0.849SerAsn: 0.849 ± 0.023	3.226SerPro: 3.226 ± 0.047	1.182SerGln: 1.182 ± 0.027	3.918SerArg: 3.918 ± 0.048	2.868SerSer: 2.868 ± 0.05	3.007SerThr: 3.007 ± 0.045	4.36SerVal: 4.36 ± 0.053	0.905SerTrp: 0.905 ± 0.023	1.254SerTyr: 1.254 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	8.728ThrAla: 8.728 ± 0.075	0.439ThrCys: 0.439 ± 0.015	3.647ThrAsp: 3.647 ± 0.052	3.321ThrGlu: 3.321 ± 0.047	1.595ThrPhe: 1.595 ± 0.03	6.782ThrGly: 6.782 ± 0.068	1.197ThrHis: 1.197 ± 0.028	1.528ThrIle: 1.528 ± 0.03	1.139ThrLys: 1.139 ± 0.032	5.735ThrLeu: 5.735 ± 0.059	0.972ThrMet: 0.972 ± 0.023	0.989ThrAsn: 0.989 ± 0.024	4.07ThrPro: 4.07 ± 0.047	1.268ThrGln: 1.268 ± 0.023	4.094ThrArg: 4.094 ± 0.045	3.076ThrSer: 3.076 ± 0.046	3.723ThrThr: 3.723 ± 0.066	6.144ThrVal: 6.144 ± 0.056	0.881ThrTrp: 0.881 ± 0.021	1.377ThrTyr: 1.377 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.294ValAla: 10.294 ± 0.085	0.785ValCys: 0.785 ± 0.02	5.056ValAsp: 5.056 ± 0.05	4.812ValGlu: 4.812 ± 0.057	2.516ValPhe: 2.516 ± 0.04	6.399ValGly: 6.399 ± 0.065	2.025ValHis: 2.025 ± 0.034	2.821ValIle: 2.821 ± 0.041	1.772ValLys: 1.772 ± 0.033	9.483ValLeu: 9.483 ± 0.091	1.476ValMet: 1.476 ± 0.03	1.723ValAsn: 1.723 ± 0.033	5.346ValPro: 5.346 ± 0.054	2.011ValGln: 2.011 ± 0.032	7.548ValArg: 7.548 ± 0.072	4.347ValSer: 4.347 ± 0.045	5.794ValThr: 5.794 ± 0.061	8.007ValVal: 8.007 ± 0.082	1.161ValTrp: 1.161 ± 0.026	1.661ValTyr: 1.661 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.619TrpAla: 1.619 ± 0.036	0.157TrpCys: 0.157 ± 0.01	0.858TrpAsp: 0.858 ± 0.021	0.761TrpGlu: 0.761 ± 0.021	0.503TrpPhe: 0.503 ± 0.016	1.092TrpGly: 1.092 ± 0.025	0.346TrpHis: 0.346 ± 0.014	0.545TrpIle: 0.545 ± 0.015	0.389TrpLys: 0.389 ± 0.013	1.696TrpLeu: 1.696 ± 0.034	0.298TrpMet: 0.298 ± 0.013	0.425TrpAsn: 0.425 ± 0.016	0.78TrpPro: 0.78 ± 0.02	0.592TrpGln: 0.592 ± 0.017	1.38TrpArg: 1.38 ± 0.031	0.914TrpSer: 0.914 ± 0.024	1.071TrpThr: 1.071 ± 0.024	0.985TrpVal: 0.985 ± 0.019	0.314TrpTrp: 0.314 ± 0.014	0.368TrpTyr: 0.368 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.784TyrAla: 2.784 ± 0.035	0.196TyrCys: 0.196 ± 0.012	1.613TyrAsp: 1.613 ± 0.038	1.28TyrGlu: 1.28 ± 0.023	0.656TyrPhe: 0.656 ± 0.019	2.374TyrGly: 2.374 ± 0.039	0.39TyrHis: 0.39 ± 0.014	0.496TyrIle: 0.496 ± 0.015	0.419TyrLys: 0.419 ± 0.016	2.1TyrLeu: 2.1 ± 0.032	0.27TyrMet: 0.27 ± 0.01	0.399TyrAsn: 0.399 ± 0.017	1.066TyrPro: 1.066 ± 0.024	0.6TyrGln: 0.6 ± 0.015	1.896TyrArg: 1.896 ± 0.033	0.969TyrSer: 0.969 ± 0.025	1.221TyrThr: 1.221 ± 0.034	1.687TyrVal: 1.687 ± 0.031	0.352TyrTrp: 0.352 ± 0.013	0.468TyrTyr: 0.468 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6337 proteins (2042203 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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