Saccharomyces arboricola (strain H-6 / AS 2.3317 / CBS 10644) (Yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces; Saccharomyces arboricola

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J8LI28|J8LI28_SACAR Vps5p OS=Saccharomyces arboricola (strain H-6 / AS 2.3317 / CBS 10644) OX=1160507 GN=SU7_3228 PE=4 SV=1
MM1 pKa = 7.34FSVEE5 pKa = 3.97STAFSGYY12 pKa = 9.07EE13 pKa = 3.83DD14 pKa = 3.78EE15 pKa = 5.15VFPLSFSNAAFEE27 pKa = 4.47PPMLSHH33 pKa = 6.75SPDD36 pKa = 3.13RR37 pKa = 11.84SSYY40 pKa = 11.29ADD42 pKa = 3.89EE43 pKa = 5.1FSQSYY48 pKa = 7.39QQEE51 pKa = 4.66LLTSPLSYY59 pKa = 10.35PIVDD63 pKa = 4.04EE64 pKa = 5.17LEE66 pKa = 3.79CAYY69 pKa = 10.73AKK71 pKa = 10.84NKK73 pKa = 8.5TGNNIIAGADD83 pKa = 3.33DD84 pKa = 5.57DD85 pKa = 6.11FIFDD89 pKa = 4.21MEE91 pKa = 4.4FSGNAASAAAASAKK105 pKa = 10.27EE106 pKa = 3.85PATASNAALANNDD119 pKa = 2.65AFANVAQQNYY129 pKa = 9.07RR130 pKa = 11.84LWLSSVV136 pKa = 3.15

Molecular weight: 14.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J8PRJ0|J8PRJ0_SACAR Autophagy-related protein 14 OS=Saccharomyces arboricola (strain H-6 / AS 2.3317 / CBS 10644) OX=1160507 GN=SU7_0209 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.11WRR6 pKa = 11.84KK7 pKa = 9.07KK8 pKa = 6.93RR9 pKa = 11.84TRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.48RR15 pKa = 11.84KK16 pKa = 8.41RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3653

0

3653

1820601

25

4902

498.4

56.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.573 ± 0.037

1.226 ± 0.013

5.998 ± 0.028

6.54 ± 0.037

4.433 ± 0.028

5.112 ± 0.037

2.144 ± 0.014

6.494 ± 0.031

7.257 ± 0.041

9.593 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.013

6.114 ± 0.042

4.336 ± 0.024

4.056 ± 0.034

4.415 ± 0.026

8.783 ± 0.055

5.818 ± 0.025

5.674 ± 0.026

1.034 ± 0.013

3.311 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski