Amycolatopsis keratiniphila

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8161 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4TGZ0|R4TGZ0_9PSEU Peptide/nickel transport system substrate-binding protein OS=Amycolatopsis keratiniphila OX=129921 GN=AORI_6991 PE=4 SV=1
MM1 pKa = 7.53QPGQPAQPDD10 pKa = 3.74YY11 pKa = 11.27GQPQYY16 pKa = 11.2GQQPPQEE23 pKa = 4.16QQSQGGGFGDD33 pKa = 3.95TPEE36 pKa = 4.37PTQVVQPVQPGSGDD50 pKa = 3.41ANATQVVQPVSGGGEE65 pKa = 4.18SPTAEE70 pKa = 4.18STQLVPPGSQPPAIPYY86 pKa = 9.46APPPSAADD94 pKa = 3.48NPAAGAYY101 pKa = 7.59GAQGGGFGQQQGFGQPQQGGFDD123 pKa = 3.91PAQGQQPGFGQPQQGFGGPPQGGFGQQPGFGGPPPGYY160 pKa = 10.08SPAAAGGGGGNQMFGLIAGGVVALLGLVALIVSFVYY196 pKa = 10.49FGDD199 pKa = 3.6ASDD202 pKa = 3.82YY203 pKa = 11.59SKK205 pKa = 11.52VFDD208 pKa = 3.6TAPNQEE214 pKa = 4.44QIDD217 pKa = 4.05KK218 pKa = 10.55FLDD221 pKa = 3.4EE222 pKa = 5.41AGLVGPGAIWFYY234 pKa = 11.48VIMLLVGSLASLAGGVGLALAGKK257 pKa = 10.34LPGTVKK263 pKa = 10.61KK264 pKa = 10.19IAPIVVAAGGALLVLFGVLLLSGSSPSSDD293 pKa = 3.96FLDD296 pKa = 3.85KK297 pKa = 11.03LPPSARR303 pKa = 11.84DD304 pKa = 3.24SAGIGGGLLHH314 pKa = 7.2LLLGIVILIVGVLGLIPATAQFVGLGGGGSVLGAPQSGQPGGFGGPPQGGFGQPGQPGQFGQPGQPGGFGQPGQPGPSSGGFPQPGQPGGFGQQPGQQGPPSGGFAQPGQPPQGGFGQAPQQPGGFGQQPGQQGPPSGGFGQPGQQPGQPPQQWW468 pKa = 2.9

Molecular weight: 45.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6HZH3|W6HZH3_9PSEU Uncharacterized protein OS=Amycolatopsis keratiniphila OX=129921 GN=AORI_P012 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 10.12GRR42 pKa = 11.84GALSAA47 pKa = 4.3

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8161

0

8161

2694571

37

11284

330.2

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.965 ± 0.039

0.734 ± 0.008

5.93 ± 0.026

5.956 ± 0.029

3.027 ± 0.017

9.338 ± 0.027

2.089 ± 0.012

3.654 ± 0.019

2.588 ± 0.021

10.512 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.01

1.97 ± 0.015

5.821 ± 0.025

2.634 ± 0.017

7.584 ± 0.027

5.267 ± 0.017

5.969 ± 0.025

8.74 ± 0.024

1.509 ± 0.013

2.005 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski