Amino acid dipepetide frequency for Gordonia phage Nyceirae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.53AlaAla: 18.53 ± 2.644
0.825AlaCys: 0.825 ± 0.222
8.252AlaAsp: 8.252 ± 0.779
7.052AlaGlu: 7.052 ± 0.818
2.176AlaPhe: 2.176 ± 0.378
12.303AlaGly: 12.303 ± 1.522
2.251AlaHis: 2.251 ± 0.423
5.251AlaIle: 5.251 ± 0.612
3.301AlaLys: 3.301 ± 0.386
9.227AlaLeu: 9.227 ± 1.108
3.076AlaMet: 3.076 ± 0.453
3.076AlaAsn: 3.076 ± 0.738
5.026AlaPro: 5.026 ± 1.067
5.101AlaGln: 5.101 ± 0.78
8.777AlaArg: 8.777 ± 0.911
5.626AlaSer: 5.626 ± 0.97
7.352AlaThr: 7.352 ± 0.716
7.427AlaVal: 7.427 ± 0.736
1.35AlaTrp: 1.35 ± 0.362
3.076AlaTyr: 3.076 ± 0.539
0.0AlaXaa: 0.0 ± 0.0
Cys
0.675CysAla: 0.675 ± 0.252
0.15CysCys: 0.15 ± 0.132
1.2CysAsp: 1.2 ± 0.363
0.45CysGlu: 0.45 ± 0.214
0.0CysPhe: 0.0 ± 0.0
1.125CysGly: 1.125 ± 0.337
0.225CysHis: 0.225 ± 0.125
0.3CysIle: 0.3 ± 0.142
0.075CysLys: 0.075 ± 0.07
0.225CysLeu: 0.225 ± 0.124
0.075CysMet: 0.075 ± 0.079
0.075CysAsn: 0.075 ± 0.093
0.375CysPro: 0.375 ± 0.169
0.075CysGln: 0.075 ± 0.068
0.825CysArg: 0.825 ± 0.387
0.6CysSer: 0.6 ± 0.177
0.675CysThr: 0.675 ± 0.221
0.825CysVal: 0.825 ± 0.26
0.225CysTrp: 0.225 ± 0.13
0.15CysTyr: 0.15 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
8.252AspAla: 8.252 ± 0.576
0.6AspCys: 0.6 ± 0.17
4.276AspAsp: 4.276 ± 0.728
4.576AspGlu: 4.576 ± 0.554
1.05AspPhe: 1.05 ± 0.252
8.777AspGly: 8.777 ± 0.718
1.275AspHis: 1.275 ± 0.431
2.251AspIle: 2.251 ± 0.339
1.8AspLys: 1.8 ± 0.426
6.602AspLeu: 6.602 ± 0.867
1.425AspMet: 1.425 ± 0.351
1.425AspAsn: 1.425 ± 0.301
6.002AspPro: 6.002 ± 0.695
2.926AspGln: 2.926 ± 0.422
4.126AspArg: 4.126 ± 0.591
3.076AspSer: 3.076 ± 0.554
3.076AspThr: 3.076 ± 0.485
3.826AspVal: 3.826 ± 0.551
1.5AspTrp: 1.5 ± 0.441
0.9AspTyr: 0.9 ± 0.223
0.0AspXaa: 0.0 ± 0.0
Glu
6.827GluAla: 6.827 ± 0.676
0.525GluCys: 0.525 ± 0.224
3.076GluAsp: 3.076 ± 0.601
2.476GluGlu: 2.476 ± 0.463
1.8GluPhe: 1.8 ± 0.395
3.301GluGly: 3.301 ± 0.473
1.65GluHis: 1.65 ± 0.298
2.776GluIle: 2.776 ± 0.432
1.725GluLys: 1.725 ± 0.323
4.951GluLeu: 4.951 ± 0.767
1.575GluMet: 1.575 ± 0.273
2.251GluAsn: 2.251 ± 0.405
1.725GluPro: 1.725 ± 0.471
2.176GluGln: 2.176 ± 0.396
5.401GluArg: 5.401 ± 0.635
3.451GluSer: 3.451 ± 0.459
1.95GluThr: 1.95 ± 0.326
4.576GluVal: 4.576 ± 0.641
2.551GluTrp: 2.551 ± 0.557
1.2GluTyr: 1.2 ± 0.231
0.0GluXaa: 0.0 ± 0.0
Phe
2.551PheAla: 2.551 ± 0.446
0.225PheCys: 0.225 ± 0.156
1.95PheAsp: 1.95 ± 0.397
1.2PheGlu: 1.2 ± 0.315
0.675PhePhe: 0.675 ± 0.23
2.326PheGly: 2.326 ± 0.455
0.45PheHis: 0.45 ± 0.191
0.825PheIle: 0.825 ± 0.239
0.825PheLys: 0.825 ± 0.239
1.575PheLeu: 1.575 ± 0.331
0.45PheMet: 0.45 ± 0.16
1.35PheAsn: 1.35 ± 0.378
1.575PhePro: 1.575 ± 0.375
0.225PheGln: 0.225 ± 0.123
1.65PheArg: 1.65 ± 0.329
1.875PheSer: 1.875 ± 0.383
2.401PheThr: 2.401 ± 0.457
1.575PheVal: 1.575 ± 0.306
0.75PheTrp: 0.75 ± 0.251
0.675PheTyr: 0.675 ± 0.252
0.0PheXaa: 0.0 ± 0.0
Gly
9.827GlyAla: 9.827 ± 1.904
0.525GlyCys: 0.525 ± 0.211
6.602GlyAsp: 6.602 ± 0.605
5.551GlyGlu: 5.551 ± 0.642
2.626GlyPhe: 2.626 ± 0.501
10.878GlyGly: 10.878 ± 1.738
1.725GlyHis: 1.725 ± 0.279
3.676GlyIle: 3.676 ± 0.694
2.401GlyLys: 2.401 ± 0.334
7.652GlyLeu: 7.652 ± 0.833
1.65GlyMet: 1.65 ± 0.375
2.251GlyAsn: 2.251 ± 0.357
4.651GlyPro: 4.651 ± 0.599
4.351GlyGln: 4.351 ± 0.71
6.152GlyArg: 6.152 ± 0.694
6.377GlySer: 6.377 ± 0.94
6.152GlyThr: 6.152 ± 0.75
6.602GlyVal: 6.602 ± 0.843
1.95GlyTrp: 1.95 ± 0.32
2.101GlyTyr: 2.101 ± 0.468
0.0GlyXaa: 0.0 ± 0.0
His
1.275HisAla: 1.275 ± 0.3
0.3HisCys: 0.3 ± 0.16
0.975HisAsp: 0.975 ± 0.223
1.125HisGlu: 1.125 ± 0.26
0.375HisPhe: 0.375 ± 0.15
1.725HisGly: 1.725 ± 0.35
0.225HisHis: 0.225 ± 0.133
0.75HisIle: 0.75 ± 0.274
0.375HisLys: 0.375 ± 0.158
2.101HisLeu: 2.101 ± 0.604
0.375HisMet: 0.375 ± 0.167
0.375HisAsn: 0.375 ± 0.162
1.65HisPro: 1.65 ± 0.407
0.375HisGln: 0.375 ± 0.157
2.176HisArg: 2.176 ± 0.478
0.825HisSer: 0.825 ± 0.256
1.2HisThr: 1.2 ± 0.239
1.05HisVal: 1.05 ± 0.232
0.45HisTrp: 0.45 ± 0.197
0.75HisTyr: 0.75 ± 0.248
0.0HisXaa: 0.0 ± 0.0
Ile
5.176IleAla: 5.176 ± 0.677
0.375IleCys: 0.375 ± 0.174
2.551IleAsp: 2.551 ± 0.333
2.626IleGlu: 2.626 ± 0.408
1.275IlePhe: 1.275 ± 0.327
3.751IleGly: 3.751 ± 0.683
1.05IleHis: 1.05 ± 0.283
1.125IleIle: 1.125 ± 0.251
1.05IleLys: 1.05 ± 0.254
2.701IleLeu: 2.701 ± 0.423
0.45IleMet: 0.45 ± 0.164
0.9IleAsn: 0.9 ± 0.309
2.251IlePro: 2.251 ± 0.406
1.35IleGln: 1.35 ± 0.314
2.851IleArg: 2.851 ± 0.395
2.776IleSer: 2.776 ± 0.493
2.776IleThr: 2.776 ± 0.391
3.526IleVal: 3.526 ± 0.538
0.6IleTrp: 0.6 ± 0.205
0.825IleTyr: 0.825 ± 0.282
0.0IleXaa: 0.0 ± 0.0
Lys
3.376LysAla: 3.376 ± 0.621
0.225LysCys: 0.225 ± 0.127
1.2LysAsp: 1.2 ± 0.256
1.125LysGlu: 1.125 ± 0.384
1.05LysPhe: 1.05 ± 0.333
1.875LysGly: 1.875 ± 0.51
0.525LysHis: 0.525 ± 0.197
0.9LysIle: 0.9 ± 0.216
1.05LysLys: 1.05 ± 0.312
2.701LysLeu: 2.701 ± 0.47
0.825LysMet: 0.825 ± 0.237
0.525LysAsn: 0.525 ± 0.176
2.401LysPro: 2.401 ± 0.428
1.725LysGln: 1.725 ± 0.527
1.8LysArg: 1.8 ± 0.451
2.251LysSer: 2.251 ± 0.405
1.95LysThr: 1.95 ± 0.275
2.176LysVal: 2.176 ± 0.571
0.75LysTrp: 0.75 ± 0.243
0.75LysTyr: 0.75 ± 0.215
0.0LysXaa: 0.0 ± 0.0
Leu
10.728LeuAla: 10.728 ± 1.044
0.3LeuCys: 0.3 ± 0.158
5.101LeuAsp: 5.101 ± 0.654
5.326LeuGlu: 5.326 ± 0.479
1.875LeuPhe: 1.875 ± 0.303
6.077LeuGly: 6.077 ± 0.637
1.725LeuHis: 1.725 ± 0.394
3.076LeuIle: 3.076 ± 0.636
3.076LeuLys: 3.076 ± 0.433
6.602LeuLeu: 6.602 ± 0.885
1.8LeuMet: 1.8 ± 0.264
1.875LeuAsn: 1.875 ± 0.297
6.002LeuPro: 6.002 ± 0.651
1.875LeuGln: 1.875 ± 0.359
6.302LeuArg: 6.302 ± 0.72
4.501LeuSer: 4.501 ± 0.412
5.626LeuThr: 5.626 ± 0.559
6.302LeuVal: 6.302 ± 0.728
0.975LeuTrp: 0.975 ± 0.302
1.275LeuTyr: 1.275 ± 0.312
0.0LeuXaa: 0.0 ± 0.0
Met
2.476MetAla: 2.476 ± 0.406
0.075MetCys: 0.075 ± 0.07
0.75MetAsp: 0.75 ± 0.179
0.75MetGlu: 0.75 ± 0.29
0.15MetPhe: 0.15 ± 0.097
1.8MetGly: 1.8 ± 0.366
0.6MetHis: 0.6 ± 0.177
0.825MetIle: 0.825 ± 0.321
0.525MetLys: 0.525 ± 0.242
1.725MetLeu: 1.725 ± 0.356
0.3MetMet: 0.3 ± 0.153
0.9MetAsn: 0.9 ± 0.256
1.5MetPro: 1.5 ± 0.345
1.05MetGln: 1.05 ± 0.355
1.725MetArg: 1.725 ± 0.394
1.575MetSer: 1.575 ± 0.305
2.626MetThr: 2.626 ± 0.476
1.725MetVal: 1.725 ± 0.319
0.45MetTrp: 0.45 ± 0.172
0.675MetTyr: 0.675 ± 0.253
0.0MetXaa: 0.0 ± 0.0
Asn
2.851AsnAla: 2.851 ± 0.501
0.15AsnCys: 0.15 ± 0.099
1.35AsnAsp: 1.35 ± 0.341
1.125AsnGlu: 1.125 ± 0.253
0.75AsnPhe: 0.75 ± 0.185
2.851AsnGly: 2.851 ± 0.49
0.45AsnHis: 0.45 ± 0.166
0.825AsnIle: 0.825 ± 0.41
0.9AsnLys: 0.9 ± 0.301
1.5AsnLeu: 1.5 ± 0.352
0.6AsnMet: 0.6 ± 0.258
0.375AsnAsn: 0.375 ± 0.141
2.251AsnPro: 2.251 ± 0.391
1.2AsnGln: 1.2 ± 0.267
2.626AsnArg: 2.626 ± 0.463
1.875AsnSer: 1.875 ± 0.387
3.001AsnThr: 3.001 ± 0.697
1.5AsnVal: 1.5 ± 0.475
0.6AsnTrp: 0.6 ± 0.263
0.675AsnTyr: 0.675 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
6.752ProAla: 6.752 ± 0.973
0.6ProCys: 0.6 ± 0.218
5.776ProAsp: 5.776 ± 0.607
3.451ProGlu: 3.451 ± 0.529
1.425ProPhe: 1.425 ± 0.294
6.152ProGly: 6.152 ± 0.525
0.75ProHis: 0.75 ± 0.262
2.251ProIle: 2.251 ± 0.492
1.65ProLys: 1.65 ± 0.301
4.126ProLeu: 4.126 ± 0.577
1.5ProMet: 1.5 ± 0.325
1.95ProAsn: 1.95 ± 0.383
4.351ProPro: 4.351 ± 0.966
2.776ProGln: 2.776 ± 0.39
3.676ProArg: 3.676 ± 0.592
3.076ProSer: 3.076 ± 0.845
3.226ProThr: 3.226 ± 0.626
3.976ProVal: 3.976 ± 0.576
1.275ProTrp: 1.275 ± 0.387
1.575ProTyr: 1.575 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
4.651GlnAla: 4.651 ± 0.75
0.075GlnCys: 0.075 ± 0.077
2.026GlnAsp: 2.026 ± 0.383
1.5GlnGlu: 1.5 ± 0.449
1.2GlnPhe: 1.2 ± 0.337
2.776GlnGly: 2.776 ± 0.477
0.6GlnHis: 0.6 ± 0.204
2.476GlnIle: 2.476 ± 0.408
0.975GlnLys: 0.975 ± 0.289
3.826GlnLeu: 3.826 ± 0.603
0.975GlnMet: 0.975 ± 0.258
0.975GlnAsn: 0.975 ± 0.28
2.326GlnPro: 2.326 ± 0.448
1.95GlnGln: 1.95 ± 0.361
3.301GlnArg: 3.301 ± 0.441
2.101GlnSer: 2.101 ± 0.46
2.551GlnThr: 2.551 ± 0.501
2.251GlnVal: 2.251 ± 0.514
0.6GlnTrp: 0.6 ± 0.233
0.75GlnTyr: 0.75 ± 0.212
0.0GlnXaa: 0.0 ± 0.0
Arg
7.052ArgAla: 7.052 ± 0.863
0.75ArgCys: 0.75 ± 0.278
5.101ArgAsp: 5.101 ± 0.731
4.426ArgGlu: 4.426 ± 0.722
2.401ArgPhe: 2.401 ± 0.384
5.551ArgGly: 5.551 ± 0.82
1.875ArgHis: 1.875 ± 0.397
3.901ArgIle: 3.901 ± 0.542
2.326ArgLys: 2.326 ± 0.357
7.127ArgLeu: 7.127 ± 0.942
2.326ArgMet: 2.326 ± 0.339
2.551ArgAsn: 2.551 ± 0.354
3.751ArgPro: 3.751 ± 0.763
2.701ArgGln: 2.701 ± 0.484
7.352ArgArg: 7.352 ± 0.839
4.351ArgSer: 4.351 ± 0.948
4.051ArgThr: 4.051 ± 0.499
5.701ArgVal: 5.701 ± 0.719
2.176ArgTrp: 2.176 ± 0.438
1.425ArgTyr: 1.425 ± 0.401
0.0ArgXaa: 0.0 ± 0.0
Ser
7.127SerAla: 7.127 ± 0.777
0.525SerCys: 0.525 ± 0.228
4.576SerAsp: 4.576 ± 0.573
2.626SerGlu: 2.626 ± 0.47
1.725SerPhe: 1.725 ± 0.346
6.452SerGly: 6.452 ± 1.271
0.3SerHis: 0.3 ± 0.147
2.326SerIle: 2.326 ± 0.481
2.251SerLys: 2.251 ± 0.358
4.351SerLeu: 4.351 ± 0.401
1.5SerMet: 1.5 ± 0.486
1.65SerAsn: 1.65 ± 0.359
3.076SerPro: 3.076 ± 0.437
1.95SerGln: 1.95 ± 0.309
3.676SerArg: 3.676 ± 0.591
1.875SerSer: 1.875 ± 0.296
4.576SerThr: 4.576 ± 0.566
4.276SerVal: 4.276 ± 0.437
0.9SerTrp: 0.9 ± 0.219
0.6SerTyr: 0.6 ± 0.201
0.0SerXaa: 0.0 ± 0.0
Thr
8.927ThrAla: 8.927 ± 0.809
0.45ThrCys: 0.45 ± 0.23
4.876ThrAsp: 4.876 ± 0.584
3.826ThrGlu: 3.826 ± 0.509
1.5ThrPhe: 1.5 ± 0.347
5.476ThrGly: 5.476 ± 0.878
1.05ThrHis: 1.05 ± 0.249
2.551ThrIle: 2.551 ± 0.536
1.575ThrLys: 1.575 ± 0.298
4.501ThrLeu: 4.501 ± 0.643
0.975ThrMet: 0.975 ± 0.214
1.575ThrAsn: 1.575 ± 0.322
5.551ThrPro: 5.551 ± 0.775
1.725ThrGln: 1.725 ± 0.322
4.201ThrArg: 4.201 ± 0.698
3.826ThrSer: 3.826 ± 0.546
5.476ThrThr: 5.476 ± 0.587
5.101ThrVal: 5.101 ± 0.562
1.35ThrTrp: 1.35 ± 0.405
1.425ThrTyr: 1.425 ± 0.33
0.0ThrXaa: 0.0 ± 0.0
Val
7.127ValAla: 7.127 ± 0.753
0.825ValCys: 0.825 ± 0.307
5.626ValAsp: 5.626 ± 0.527
4.651ValGlu: 4.651 ± 0.692
2.026ValPhe: 2.026 ± 0.475
6.527ValGly: 6.527 ± 0.62
0.975ValHis: 0.975 ± 0.312
2.251ValIle: 2.251 ± 0.4
1.95ValLys: 1.95 ± 0.346
5.551ValLeu: 5.551 ± 0.584
1.425ValMet: 1.425 ± 0.372
2.026ValAsn: 2.026 ± 0.399
4.351ValPro: 4.351 ± 0.646
2.326ValGln: 2.326 ± 0.498
6.452ValArg: 6.452 ± 0.773
3.676ValSer: 3.676 ± 0.617
5.401ValThr: 5.401 ± 0.871
5.551ValVal: 5.551 ± 0.843
1.875ValTrp: 1.875 ± 0.441
1.575ValTyr: 1.575 ± 0.533
0.0ValXaa: 0.0 ± 0.0
Trp
2.176TrpAla: 2.176 ± 0.347
0.6TrpCys: 0.6 ± 0.214
1.05TrpAsp: 1.05 ± 0.377
1.05TrpGlu: 1.05 ± 0.298
0.525TrpPhe: 0.525 ± 0.196
1.875TrpGly: 1.875 ± 0.363
0.525TrpHis: 0.525 ± 0.175
0.675TrpIle: 0.675 ± 0.228
0.525TrpLys: 0.525 ± 0.177
1.95TrpLeu: 1.95 ± 0.43
0.525TrpMet: 0.525 ± 0.167
0.75TrpAsn: 0.75 ± 0.275
0.675TrpPro: 0.675 ± 0.253
1.05TrpGln: 1.05 ± 0.26
1.8TrpArg: 1.8 ± 0.341
1.125TrpSer: 1.125 ± 0.386
0.975TrpThr: 0.975 ± 0.264
2.251TrpVal: 2.251 ± 0.443
0.525TrpTrp: 0.525 ± 0.188
0.525TrpTyr: 0.525 ± 0.27
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.776TyrAla: 2.776 ± 0.524
0.225TyrCys: 0.225 ± 0.15
1.425TyrAsp: 1.425 ± 0.3
1.05TyrGlu: 1.05 ± 0.289
0.525TyrPhe: 0.525 ± 0.187
2.101TyrGly: 2.101 ± 0.489
0.225TyrHis: 0.225 ± 0.11
0.975TyrIle: 0.975 ± 0.205
0.825TyrLys: 0.825 ± 0.264
1.425TyrLeu: 1.425 ± 0.347
0.3TyrMet: 0.3 ± 0.187
0.6TyrAsn: 0.6 ± 0.233
0.825TyrPro: 0.825 ± 0.251
0.975TyrGln: 0.975 ± 0.227
2.026TyrArg: 2.026 ± 0.421
1.5TyrSer: 1.5 ± 0.305
0.9TyrThr: 0.9 ± 0.299
1.95TyrVal: 1.95 ± 0.434
0.3TyrTrp: 0.3 ± 0.146
0.75TyrTyr: 0.75 ± 0.276
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 60 proteins (13331 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski