Amino acid dipepetide frequency for Streptomyces phage Braelyn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.656AlaAla: 7.656 ± 0.858
0.818AlaCys: 0.818 ± 0.193
4.851AlaAsp: 4.851 ± 0.413
5.757AlaGlu: 5.757 ± 0.519
3.156AlaPhe: 3.156 ± 0.287
5.464AlaGly: 5.464 ± 0.514
1.549AlaHis: 1.549 ± 0.212
5.172AlaIle: 5.172 ± 0.42
5.318AlaLys: 5.318 ± 0.414
6.779AlaLeu: 6.779 ± 0.656
2.513AlaMet: 2.513 ± 0.277
3.477AlaAsn: 3.477 ± 0.41
2.951AlaPro: 2.951 ± 0.411
3.039AlaGln: 3.039 ± 0.371
4.675AlaArg: 4.675 ± 0.359
4.617AlaSer: 4.617 ± 0.445
5.201AlaThr: 5.201 ± 0.587
5.552AlaVal: 5.552 ± 0.36
1.636AlaTrp: 1.636 ± 0.227
3.331AlaTyr: 3.331 ± 0.289
0.0AlaXaa: 0.0 ± 0.0
Cys
0.584CysAla: 0.584 ± 0.151
0.146CysCys: 0.146 ± 0.073
0.555CysAsp: 0.555 ± 0.14
0.701CysGlu: 0.701 ± 0.158
0.205CysPhe: 0.205 ± 0.078
1.257CysGly: 1.257 ± 0.231
0.321CysHis: 0.321 ± 0.1
0.526CysIle: 0.526 ± 0.139
0.847CysLys: 0.847 ± 0.161
0.584CysLeu: 0.584 ± 0.146
0.205CysMet: 0.205 ± 0.076
0.468CysAsn: 0.468 ± 0.135
0.555CysPro: 0.555 ± 0.137
0.263CysGln: 0.263 ± 0.087
0.701CysArg: 0.701 ± 0.139
0.584CysSer: 0.584 ± 0.14
0.351CysThr: 0.351 ± 0.1
0.731CysVal: 0.731 ± 0.146
0.117CysTrp: 0.117 ± 0.06
0.438CysTyr: 0.438 ± 0.128
0.0CysXaa: 0.0 ± 0.0
Asp
5.757AspAla: 5.757 ± 0.46
0.555AspCys: 0.555 ± 0.134
4.295AspAsp: 4.295 ± 0.42
5.64AspGlu: 5.64 ± 0.511
2.864AspPhe: 2.864 ± 0.34
5.494AspGly: 5.494 ± 0.491
1.169AspHis: 1.169 ± 0.198
3.653AspIle: 3.653 ± 0.374
3.77AspLys: 3.77 ± 0.391
4.558AspLeu: 4.558 ± 0.355
2.455AspMet: 2.455 ± 0.292
2.571AspAsn: 2.571 ± 0.254
2.192AspPro: 2.192 ± 0.285
1.666AspGln: 1.666 ± 0.213
2.776AspArg: 2.776 ± 0.289
3.74AspSer: 3.74 ± 0.301
3.39AspThr: 3.39 ± 0.407
4.062AspVal: 4.062 ± 0.451
1.607AspTrp: 1.607 ± 0.202
2.601AspTyr: 2.601 ± 0.275
0.0AspXaa: 0.0 ± 0.0
Glu
5.786GluAla: 5.786 ± 0.534
0.584GluCys: 0.584 ± 0.132
4.149GluAsp: 4.149 ± 0.396
5.26GluGlu: 5.26 ± 0.521
2.951GluPhe: 2.951 ± 0.287
4.588GluGly: 4.588 ± 0.434
1.666GluHis: 1.666 ± 0.271
4.442GluIle: 4.442 ± 0.378
4.675GluLys: 4.675 ± 0.417
5.172GluLeu: 5.172 ± 0.48
1.958GluMet: 1.958 ± 0.188
3.01GluAsn: 3.01 ± 0.275
2.221GluPro: 2.221 ± 0.346
2.981GluGln: 2.981 ± 0.39
4.354GluArg: 4.354 ± 0.395
3.477GluSer: 3.477 ± 0.318
3.77GluThr: 3.77 ± 0.408
5.464GluVal: 5.464 ± 0.464
1.607GluTrp: 1.607 ± 0.25
3.039GluTyr: 3.039 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
2.834PheAla: 2.834 ± 0.301
0.38PheCys: 0.38 ± 0.117
3.74PheAsp: 3.74 ± 0.287
3.623PheGlu: 3.623 ± 0.3
1.607PhePhe: 1.607 ± 0.24
2.776PheGly: 2.776 ± 0.316
0.643PheHis: 0.643 ± 0.143
1.782PheIle: 1.782 ± 0.231
2.133PheLys: 2.133 ± 0.233
2.045PheLeu: 2.045 ± 0.27
0.964PheMet: 0.964 ± 0.151
1.958PheAsn: 1.958 ± 0.236
0.994PhePro: 0.994 ± 0.18
1.227PheGln: 1.227 ± 0.225
1.812PheArg: 1.812 ± 0.231
2.63PheSer: 2.63 ± 0.289
2.542PheThr: 2.542 ± 0.262
2.63PheVal: 2.63 ± 0.26
0.497PheTrp: 0.497 ± 0.102
1.315PheTyr: 1.315 ± 0.165
0.0PheXaa: 0.0 ± 0.0
Gly
5.055GlyAla: 5.055 ± 0.483
0.789GlyCys: 0.789 ± 0.136
4.442GlyAsp: 4.442 ± 0.387
4.266GlyGlu: 4.266 ± 0.403
3.156GlyPhe: 3.156 ± 0.256
5.61GlyGly: 5.61 ± 0.554
1.899GlyHis: 1.899 ± 0.262
4.354GlyIle: 4.354 ± 0.389
5.231GlyLys: 5.231 ± 0.395
5.552GlyLeu: 5.552 ± 0.549
2.133GlyMet: 2.133 ± 0.23
3.653GlyAsn: 3.653 ± 0.313
2.075GlyPro: 2.075 ± 0.249
2.455GlyGln: 2.455 ± 0.288
4.003GlyArg: 4.003 ± 0.398
4.149GlySer: 4.149 ± 0.499
5.347GlyThr: 5.347 ± 0.692
6.136GlyVal: 6.136 ± 0.42
1.344GlyTrp: 1.344 ± 0.192
2.805GlyTyr: 2.805 ± 0.278
0.0GlyXaa: 0.0 ± 0.0
His
1.344HisAla: 1.344 ± 0.208
0.321HisCys: 0.321 ± 0.106
1.169HisAsp: 1.169 ± 0.177
1.169HisGlu: 1.169 ± 0.226
0.789HisPhe: 0.789 ± 0.155
1.519HisGly: 1.519 ± 0.21
0.643HisHis: 0.643 ± 0.14
1.286HisIle: 1.286 ± 0.21
1.315HisLys: 1.315 ± 0.2
1.49HisLeu: 1.49 ± 0.175
0.526HisMet: 0.526 ± 0.142
0.847HisAsn: 0.847 ± 0.147
0.877HisPro: 0.877 ± 0.189
0.38HisGln: 0.38 ± 0.122
1.315HisArg: 1.315 ± 0.225
1.023HisSer: 1.023 ± 0.166
0.877HisThr: 0.877 ± 0.172
1.198HisVal: 1.198 ± 0.234
0.321HisTrp: 0.321 ± 0.094
0.994HisTyr: 0.994 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
4.412IleAla: 4.412 ± 0.348
0.672IleCys: 0.672 ± 0.163
4.091IleAsp: 4.091 ± 0.305
4.471IleGlu: 4.471 ± 0.407
1.461IlePhe: 1.461 ± 0.235
3.36IleGly: 3.36 ± 0.346
0.994IleHis: 0.994 ± 0.188
2.367IleIle: 2.367 ± 0.255
3.711IleLys: 3.711 ± 0.356
3.682IleLeu: 3.682 ± 0.477
1.257IleMet: 1.257 ± 0.19
1.753IleAsn: 1.753 ± 0.286
2.338IlePro: 2.338 ± 0.284
2.016IleGln: 2.016 ± 0.309
3.185IleArg: 3.185 ± 0.325
3.536IleSer: 3.536 ± 0.32
2.805IleThr: 2.805 ± 0.303
4.325IleVal: 4.325 ± 0.398
0.818IleTrp: 0.818 ± 0.172
1.666IleTyr: 1.666 ± 0.255
0.0IleXaa: 0.0 ± 0.0
Lys
6.312LysAla: 6.312 ± 0.496
0.935LysCys: 0.935 ± 0.2
3.945LysAsp: 3.945 ± 0.485
4.12LysGlu: 4.12 ± 0.362
2.279LysPhe: 2.279 ± 0.272
4.208LysGly: 4.208 ± 0.374
1.14LysHis: 1.14 ± 0.195
2.893LysIle: 2.893 ± 0.31
4.237LysLys: 4.237 ± 0.443
4.763LysLeu: 4.763 ± 0.337
2.221LysMet: 2.221 ± 0.244
3.419LysAsn: 3.419 ± 0.314
2.338LysPro: 2.338 ± 0.274
2.542LysGln: 2.542 ± 0.315
3.945LysArg: 3.945 ± 0.411
3.565LysSer: 3.565 ± 0.367
4.003LysThr: 4.003 ± 0.319
4.208LysVal: 4.208 ± 0.339
1.198LysTrp: 1.198 ± 0.174
2.425LysTyr: 2.425 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
7.042LeuAla: 7.042 ± 0.457
0.789LeuCys: 0.789 ± 0.182
5.318LeuAsp: 5.318 ± 0.415
5.435LeuGlu: 5.435 ± 0.446
2.542LeuPhe: 2.542 ± 0.315
4.734LeuGly: 4.734 ± 0.38
1.432LeuHis: 1.432 ± 0.219
4.091LeuIle: 4.091 ± 0.318
4.588LeuLys: 4.588 ± 0.385
5.172LeuLeu: 5.172 ± 0.488
1.782LeuMet: 1.782 ± 0.251
2.922LeuAsn: 2.922 ± 0.324
3.01LeuPro: 3.01 ± 0.265
1.841LeuGln: 1.841 ± 0.253
4.003LeuArg: 4.003 ± 0.345
4.646LeuSer: 4.646 ± 0.305
4.997LeuThr: 4.997 ± 0.388
4.675LeuVal: 4.675 ± 0.44
1.49LeuTrp: 1.49 ± 0.182
2.396LeuTyr: 2.396 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
3.185MetAla: 3.185 ± 0.297
0.292MetCys: 0.292 ± 0.08
1.49MetAsp: 1.49 ± 0.234
1.519MetGlu: 1.519 ± 0.206
0.76MetPhe: 0.76 ± 0.176
2.367MetGly: 2.367 ± 0.344
0.555MetHis: 0.555 ± 0.115
1.315MetIle: 1.315 ± 0.218
1.958MetLys: 1.958 ± 0.202
1.578MetLeu: 1.578 ± 0.235
0.526MetMet: 0.526 ± 0.136
1.14MetAsn: 1.14 ± 0.173
1.198MetPro: 1.198 ± 0.192
1.14MetGln: 1.14 ± 0.25
2.279MetArg: 2.279 ± 0.241
2.016MetSer: 2.016 ± 0.228
2.192MetThr: 2.192 ± 0.238
1.812MetVal: 1.812 ± 0.28
0.292MetTrp: 0.292 ± 0.089
0.818MetTyr: 0.818 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
3.711AsnAla: 3.711 ± 0.425
0.321AsnCys: 0.321 ± 0.083
2.805AsnAsp: 2.805 ± 0.262
2.922AsnGlu: 2.922 ± 0.362
1.607AsnPhe: 1.607 ± 0.184
4.12AsnGly: 4.12 ± 0.392
0.964AsnHis: 0.964 ± 0.163
2.075AsnIle: 2.075 ± 0.242
3.01AsnLys: 3.01 ± 0.34
3.623AsnLeu: 3.623 ± 0.256
1.14AsnMet: 1.14 ± 0.193
1.841AsnAsn: 1.841 ± 0.283
1.841AsnPro: 1.841 ± 0.248
1.286AsnGln: 1.286 ± 0.211
2.571AsnArg: 2.571 ± 0.311
2.221AsnSer: 2.221 ± 0.332
2.659AsnThr: 2.659 ± 0.388
3.273AsnVal: 3.273 ± 0.308
0.731AsnTrp: 0.731 ± 0.165
1.373AsnTyr: 1.373 ± 0.2
0.0AsnXaa: 0.0 ± 0.0
Pro
2.864ProAla: 2.864 ± 0.344
0.351ProCys: 0.351 ± 0.105
2.688ProAsp: 2.688 ± 0.328
2.63ProGlu: 2.63 ± 0.297
1.578ProPhe: 1.578 ± 0.208
3.448ProGly: 3.448 ± 0.337
0.701ProHis: 0.701 ± 0.138
1.636ProIle: 1.636 ± 0.211
2.455ProLys: 2.455 ± 0.279
2.25ProLeu: 2.25 ± 0.271
0.877ProMet: 0.877 ± 0.146
1.724ProAsn: 1.724 ± 0.234
1.14ProPro: 1.14 ± 0.222
0.906ProGln: 0.906 ± 0.172
2.045ProArg: 2.045 ± 0.27
2.308ProSer: 2.308 ± 0.351
2.455ProThr: 2.455 ± 0.45
3.594ProVal: 3.594 ± 0.349
0.438ProTrp: 0.438 ± 0.13
1.257ProTyr: 1.257 ± 0.255
0.0ProXaa: 0.0 ± 0.0
Gln
3.214GlnAla: 3.214 ± 0.561
0.409GlnCys: 0.409 ± 0.116
1.461GlnAsp: 1.461 ± 0.173
2.542GlnGlu: 2.542 ± 0.291
1.403GlnPhe: 1.403 ± 0.213
1.841GlnGly: 1.841 ± 0.254
0.526GlnHis: 0.526 ± 0.163
1.49GlnIle: 1.49 ± 0.198
2.308GlnLys: 2.308 ± 0.289
2.981GlnLeu: 2.981 ± 0.279
1.519GlnMet: 1.519 ± 0.209
1.49GlnAsn: 1.49 ± 0.243
1.11GlnPro: 1.11 ± 0.19
1.052GlnGln: 1.052 ± 0.286
1.987GlnArg: 1.987 ± 0.293
1.899GlnSer: 1.899 ± 0.2
1.666GlnThr: 1.666 ± 0.234
2.484GlnVal: 2.484 ± 0.236
0.555GlnTrp: 0.555 ± 0.104
1.081GlnTyr: 1.081 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
4.646ArgAla: 4.646 ± 0.52
0.438ArgCys: 0.438 ± 0.143
3.682ArgAsp: 3.682 ± 0.36
3.74ArgGlu: 3.74 ± 0.342
2.542ArgPhe: 2.542 ± 0.33
4.208ArgGly: 4.208 ± 0.308
0.818ArgHis: 0.818 ± 0.187
3.36ArgIle: 3.36 ± 0.334
3.711ArgLys: 3.711 ± 0.422
3.974ArgLeu: 3.974 ± 0.287
1.753ArgMet: 1.753 ± 0.241
2.834ArgAsn: 2.834 ± 0.263
1.812ArgPro: 1.812 ± 0.21
1.899ArgGln: 1.899 ± 0.284
3.565ArgArg: 3.565 ± 0.482
2.834ArgSer: 2.834 ± 0.26
2.601ArgThr: 2.601 ± 0.27
4.237ArgVal: 4.237 ± 0.364
1.286ArgTrp: 1.286 ± 0.217
2.571ArgTyr: 2.571 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
4.588SerAla: 4.588 ± 0.508
0.438SerCys: 0.438 ± 0.131
3.477SerAsp: 3.477 ± 0.318
3.653SerGlu: 3.653 ± 0.328
2.484SerPhe: 2.484 ± 0.271
5.201SerGly: 5.201 ± 0.515
1.14SerHis: 1.14 ± 0.176
3.185SerIle: 3.185 ± 0.344
4.003SerLys: 4.003 ± 0.403
4.675SerLeu: 4.675 ± 0.419
1.841SerMet: 1.841 ± 0.223
2.834SerAsn: 2.834 ± 0.336
1.958SerPro: 1.958 ± 0.257
1.987SerGln: 1.987 ± 0.213
3.127SerArg: 3.127 ± 0.328
3.419SerSer: 3.419 ± 0.441
3.39SerThr: 3.39 ± 0.632
4.558SerVal: 4.558 ± 0.498
1.403SerTrp: 1.403 ± 0.196
1.87SerTyr: 1.87 ± 0.253
0.0SerXaa: 0.0 ± 0.0
Thr
4.792ThrAla: 4.792 ± 0.491
0.672ThrCys: 0.672 ± 0.142
4.208ThrAsp: 4.208 ± 0.306
4.003ThrGlu: 4.003 ± 0.339
2.016ThrPhe: 2.016 ± 0.224
4.997ThrGly: 4.997 ± 0.516
0.994ThrHis: 0.994 ± 0.175
3.156ThrIle: 3.156 ± 0.33
3.302ThrLys: 3.302 ± 0.303
4.12ThrLeu: 4.12 ± 0.373
1.315ThrMet: 1.315 ± 0.198
2.718ThrAsn: 2.718 ± 0.429
3.273ThrPro: 3.273 ± 0.402
1.753ThrGln: 1.753 ± 0.245
2.659ThrArg: 2.659 ± 0.312
3.916ThrSer: 3.916 ± 0.378
4.179ThrThr: 4.179 ± 0.772
4.938ThrVal: 4.938 ± 0.548
1.315ThrTrp: 1.315 ± 0.196
1.987ThrTyr: 1.987 ± 0.244
0.0ThrXaa: 0.0 ± 0.0
Val
5.464ValAla: 5.464 ± 0.416
0.789ValCys: 0.789 ± 0.145
4.997ValAsp: 4.997 ± 0.479
5.055ValGlu: 5.055 ± 0.473
2.659ValPhe: 2.659 ± 0.275
4.705ValGly: 4.705 ± 0.356
1.373ValHis: 1.373 ± 0.225
3.828ValIle: 3.828 ± 0.385
4.997ValLys: 4.997 ± 0.425
4.88ValLeu: 4.88 ± 0.47
1.929ValMet: 1.929 ± 0.244
2.776ValAsn: 2.776 ± 0.371
3.36ValPro: 3.36 ± 0.354
2.571ValGln: 2.571 ± 0.326
4.588ValArg: 4.588 ± 0.347
5.026ValSer: 5.026 ± 0.374
4.149ValThr: 4.149 ± 0.473
5.581ValVal: 5.581 ± 0.387
1.169ValTrp: 1.169 ± 0.208
2.951ValTyr: 2.951 ± 0.337
0.0ValXaa: 0.0 ± 0.0
Trp
1.286TrpAla: 1.286 ± 0.201
0.175TrpCys: 0.175 ± 0.073
1.023TrpAsp: 1.023 ± 0.194
1.929TrpGlu: 1.929 ± 0.257
0.76TrpPhe: 0.76 ± 0.14
1.636TrpGly: 1.636 ± 0.213
0.468TrpHis: 0.468 ± 0.139
0.731TrpIle: 0.731 ± 0.148
1.081TrpLys: 1.081 ± 0.19
1.753TrpLeu: 1.753 ± 0.258
0.497TrpMet: 0.497 ± 0.131
0.935TrpAsn: 0.935 ± 0.166
0.526TrpPro: 0.526 ± 0.146
0.818TrpGln: 0.818 ± 0.18
0.76TrpArg: 0.76 ± 0.11
1.11TrpSer: 1.11 ± 0.163
1.198TrpThr: 1.198 ± 0.215
1.081TrpVal: 1.081 ± 0.161
0.526TrpTrp: 0.526 ± 0.122
0.76TrpTyr: 0.76 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.981TyrAla: 2.981 ± 0.311
0.263TyrCys: 0.263 ± 0.101
2.425TyrAsp: 2.425 ± 0.301
2.718TyrGlu: 2.718 ± 0.3
1.315TyrPhe: 1.315 ± 0.207
2.922TyrGly: 2.922 ± 0.315
0.438TyrHis: 0.438 ± 0.106
1.578TyrIle: 1.578 ± 0.207
2.045TyrLys: 2.045 ± 0.264
3.273TyrLeu: 3.273 ± 0.374
0.994TyrMet: 0.994 ± 0.184
1.724TyrAsn: 1.724 ± 0.232
1.578TyrPro: 1.578 ± 0.262
1.198TyrGln: 1.198 ± 0.166
2.162TyrArg: 2.162 ± 0.241
2.513TyrSer: 2.513 ± 0.25
2.542TyrThr: 2.542 ± 0.24
2.308TyrVal: 2.308 ± 0.263
0.701TyrTrp: 0.701 ± 0.138
1.344TyrTyr: 1.344 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 206 proteins (34223 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski