Amino acid dipepetide frequency for Streptomyces phage Alderaan

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
26.291AlaAla: 26.291 ± 2.346
1.147AlaCys: 1.147 ± 0.377
10.994AlaAsp: 10.994 ± 1.116
13.576AlaGlu: 13.576 ± 1.549
3.155AlaPhe: 3.155 ± 0.469
11.568AlaGly: 11.568 ± 1.135
2.39AlaHis: 2.39 ± 0.388
4.876AlaIle: 4.876 ± 0.697
2.39AlaLys: 2.39 ± 0.419
13.289AlaLeu: 13.289 ± 1.719
4.207AlaMet: 4.207 ± 0.664
2.008AlaAsn: 2.008 ± 0.418
8.604AlaPro: 8.604 ± 1.353
4.589AlaGln: 4.589 ± 0.617
14.818AlaArg: 14.818 ± 1.414
5.067AlaSer: 5.067 ± 0.563
11.09AlaThr: 11.09 ± 1.1
10.421AlaVal: 10.421 ± 0.971
2.199AlaTrp: 2.199 ± 0.414
2.868AlaTyr: 2.868 ± 0.492
0.0AlaXaa: 0.0 ± 0.0
Cys
0.382CysAla: 0.382 ± 0.185
0.0CysCys: 0.0 ± 0.0
0.574CysAsp: 0.574 ± 0.271
0.287CysGlu: 0.287 ± 0.142
0.096CysPhe: 0.096 ± 0.1
0.382CysGly: 0.382 ± 0.189
0.287CysHis: 0.287 ± 0.222
0.0CysIle: 0.0 ± 0.0
0.191CysLys: 0.191 ± 0.129
0.287CysLeu: 0.287 ± 0.165
0.191CysMet: 0.191 ± 0.184
0.191CysAsn: 0.191 ± 0.126
0.86CysPro: 0.86 ± 0.244
0.287CysGln: 0.287 ± 0.195
0.956CysArg: 0.956 ± 0.367
0.287CysSer: 0.287 ± 0.157
0.287CysThr: 0.287 ± 0.162
0.191CysVal: 0.191 ± 0.144
0.096CysTrp: 0.096 ± 0.099
0.096CysTyr: 0.096 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
9.943AspAla: 9.943 ± 1.134
0.287AspCys: 0.287 ± 0.168
5.258AspAsp: 5.258 ± 0.925
5.067AspGlu: 5.067 ± 1.042
1.052AspPhe: 1.052 ± 0.311
6.405AspGly: 6.405 ± 0.868
1.052AspHis: 1.052 ± 0.347
1.625AspIle: 1.625 ± 0.337
1.912AspLys: 1.912 ± 0.356
6.214AspLeu: 6.214 ± 0.738
0.669AspMet: 0.669 ± 0.196
1.434AspAsn: 1.434 ± 0.422
4.685AspPro: 4.685 ± 0.838
3.25AspGln: 3.25 ± 0.608
6.692AspArg: 6.692 ± 1.083
2.199AspSer: 2.199 ± 0.47
3.92AspThr: 3.92 ± 0.622
3.633AspVal: 3.633 ± 0.621
1.052AspTrp: 1.052 ± 0.299
0.956AspTyr: 0.956 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
9.082GluAla: 9.082 ± 1.096
0.382GluCys: 0.382 ± 0.178
3.346GluAsp: 3.346 ± 0.53
3.155GluGlu: 3.155 ± 0.619
1.147GluPhe: 1.147 ± 0.332
4.207GluGly: 4.207 ± 0.6
2.486GluHis: 2.486 ± 0.468
1.53GluIle: 1.53 ± 0.327
0.765GluLys: 0.765 ± 0.231
4.398GluLeu: 4.398 ± 0.67
0.765GluMet: 0.765 ± 0.312
1.338GluAsn: 1.338 ± 0.375
2.486GluPro: 2.486 ± 0.536
6.597GluGln: 6.597 ± 0.96
5.354GluArg: 5.354 ± 0.863
2.486GluSer: 2.486 ± 0.476
2.964GluThr: 2.964 ± 0.469
3.059GluVal: 3.059 ± 0.708
2.008GluTrp: 2.008 ± 0.322
1.052GluTyr: 1.052 ± 0.33
0.0GluXaa: 0.0 ± 0.0
Phe
3.346PheAla: 3.346 ± 0.562
0.096PheCys: 0.096 ± 0.094
1.052PheAsp: 1.052 ± 0.349
1.53PheGlu: 1.53 ± 0.314
0.669PhePhe: 0.669 ± 0.222
2.294PheGly: 2.294 ± 0.492
0.287PheHis: 0.287 ± 0.222
0.574PheIle: 0.574 ± 0.209
0.382PheLys: 0.382 ± 0.217
1.434PheLeu: 1.434 ± 0.44
0.382PheMet: 0.382 ± 0.192
0.382PheAsn: 0.382 ± 0.239
0.765PhePro: 0.765 ± 0.22
0.287PheGln: 0.287 ± 0.155
1.816PheArg: 1.816 ± 0.372
0.765PheSer: 0.765 ± 0.237
1.53PheThr: 1.53 ± 0.369
0.574PheVal: 0.574 ± 0.219
0.574PheTrp: 0.574 ± 0.205
0.287PheTyr: 0.287 ± 0.163
0.0PheXaa: 0.0 ± 0.0
Gly
11.472GlyAla: 11.472 ± 1.537
0.382GlyCys: 0.382 ± 0.187
5.641GlyAsp: 5.641 ± 0.625
4.589GlyGlu: 4.589 ± 0.763
1.625GlyPhe: 1.625 ± 0.338
7.075GlyGly: 7.075 ± 1.203
1.243GlyHis: 1.243 ± 0.43
4.302GlyIle: 4.302 ± 0.899
2.39GlyLys: 2.39 ± 0.706
6.501GlyLeu: 6.501 ± 1.158
1.816GlyMet: 1.816 ± 0.448
2.772GlyAsn: 2.772 ± 0.444
5.449GlyPro: 5.449 ± 0.854
3.155GlyGln: 3.155 ± 0.704
7.744GlyArg: 7.744 ± 0.721
4.015GlySer: 4.015 ± 0.703
6.405GlyThr: 6.405 ± 1.048
4.111GlyVal: 4.111 ± 0.622
1.625GlyTrp: 1.625 ± 0.475
1.721GlyTyr: 1.721 ± 0.427
0.0GlyXaa: 0.0 ± 0.0
His
2.772HisAla: 2.772 ± 0.658
0.191HisCys: 0.191 ± 0.141
0.956HisAsp: 0.956 ± 0.339
0.956HisGlu: 0.956 ± 0.299
0.669HisPhe: 0.669 ± 0.33
1.721HisGly: 1.721 ± 0.427
0.382HisHis: 0.382 ± 0.186
0.191HisIle: 0.191 ± 0.11
0.574HisLys: 0.574 ± 0.228
1.721HisLeu: 1.721 ± 0.477
0.287HisMet: 0.287 ± 0.149
0.287HisAsn: 0.287 ± 0.144
1.434HisPro: 1.434 ± 0.358
0.956HisGln: 0.956 ± 0.263
2.39HisArg: 2.39 ± 0.497
0.956HisSer: 0.956 ± 0.398
1.338HisThr: 1.338 ± 0.362
1.052HisVal: 1.052 ± 0.322
0.574HisTrp: 0.574 ± 0.302
0.382HisTyr: 0.382 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
4.685IleAla: 4.685 ± 0.649
0.096IleCys: 0.096 ± 0.088
2.103IleAsp: 2.103 ± 0.547
2.868IleGlu: 2.868 ± 0.485
0.382IlePhe: 0.382 ± 0.227
2.294IleGly: 2.294 ± 0.558
0.382IleHis: 0.382 ± 0.233
0.765IleIle: 0.765 ± 0.311
1.243IleLys: 1.243 ± 0.47
2.964IleLeu: 2.964 ± 0.494
0.287IleMet: 0.287 ± 0.159
1.052IleAsn: 1.052 ± 0.383
2.581IlePro: 2.581 ± 0.474
1.721IleGln: 1.721 ± 0.425
2.677IleArg: 2.677 ± 0.418
0.574IleSer: 0.574 ± 0.279
2.868IleThr: 2.868 ± 0.498
2.772IleVal: 2.772 ± 0.524
0.382IleTrp: 0.382 ± 0.183
0.382IleTyr: 0.382 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
4.111LysAla: 4.111 ± 0.78
0.096LysCys: 0.096 ± 0.088
1.052LysAsp: 1.052 ± 0.276
0.478LysGlu: 0.478 ± 0.185
0.574LysPhe: 0.574 ± 0.288
1.721LysGly: 1.721 ± 0.534
1.147LysHis: 1.147 ± 0.342
0.478LysIle: 0.478 ± 0.188
0.765LysLys: 0.765 ± 0.286
0.86LysLeu: 0.86 ± 0.265
0.287LysMet: 0.287 ± 0.214
0.287LysAsn: 0.287 ± 0.184
1.434LysPro: 1.434 ± 0.658
1.243LysGln: 1.243 ± 0.351
2.103LysArg: 2.103 ± 0.417
0.765LysSer: 0.765 ± 0.228
1.147LysThr: 1.147 ± 0.357
0.956LysVal: 0.956 ± 0.284
0.382LysTrp: 0.382 ± 0.177
0.574LysTyr: 0.574 ± 0.176
0.0LysXaa: 0.0 ± 0.0
Leu
14.818LeuAla: 14.818 ± 1.507
0.478LeuCys: 0.478 ± 0.203
8.7LeuAsp: 8.7 ± 1.104
2.199LeuGlu: 2.199 ± 0.483
2.008LeuPhe: 2.008 ± 0.368
7.075LeuGly: 7.075 ± 0.919
1.816LeuHis: 1.816 ± 0.433
3.155LeuIle: 3.155 ± 0.473
0.86LeuLys: 0.86 ± 0.279
7.935LeuLeu: 7.935 ± 1.043
0.956LeuMet: 0.956 ± 0.599
1.434LeuAsn: 1.434 ± 0.35
4.78LeuPro: 4.78 ± 0.526
1.721LeuGln: 1.721 ± 0.332
6.214LeuArg: 6.214 ± 0.698
3.633LeuSer: 3.633 ± 0.505
7.648LeuThr: 7.648 ± 0.771
3.92LeuVal: 3.92 ± 0.725
1.338LeuTrp: 1.338 ± 0.365
2.199LeuTyr: 2.199 ± 0.368
0.0LeuXaa: 0.0 ± 0.0
Met
2.772MetAla: 2.772 ± 0.691
0.191MetCys: 0.191 ± 0.116
1.147MetAsp: 1.147 ± 0.303
0.478MetGlu: 0.478 ± 0.204
0.478MetPhe: 0.478 ± 0.266
1.147MetGly: 1.147 ± 0.51
0.287MetHis: 0.287 ± 0.165
0.669MetIle: 0.669 ± 0.212
0.287MetLys: 0.287 ± 0.153
1.147MetLeu: 1.147 ± 0.314
0.287MetMet: 0.287 ± 0.197
0.574MetAsn: 0.574 ± 0.245
1.052MetPro: 1.052 ± 0.324
1.147MetGln: 1.147 ± 0.59
1.816MetArg: 1.816 ± 0.419
2.199MetSer: 2.199 ± 0.386
1.721MetThr: 1.721 ± 0.431
1.052MetVal: 1.052 ± 0.272
0.287MetTrp: 0.287 ± 0.191
0.191MetTyr: 0.191 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
2.486AsnAla: 2.486 ± 0.422
0.191AsnCys: 0.191 ± 0.119
0.765AsnAsp: 0.765 ± 0.263
0.956AsnGlu: 0.956 ± 0.306
0.287AsnPhe: 0.287 ± 0.177
2.964AsnGly: 2.964 ± 0.613
0.382AsnHis: 0.382 ± 0.227
0.382AsnIle: 0.382 ± 0.176
0.287AsnLys: 0.287 ± 0.216
1.147AsnLeu: 1.147 ± 0.276
0.574AsnMet: 0.574 ± 0.308
0.287AsnAsn: 0.287 ± 0.166
1.721AsnPro: 1.721 ± 0.503
1.052AsnGln: 1.052 ± 0.286
2.199AsnArg: 2.199 ± 0.535
0.86AsnSer: 0.86 ± 0.31
1.53AsnThr: 1.53 ± 0.391
1.816AsnVal: 1.816 ± 0.403
0.287AsnTrp: 0.287 ± 0.259
0.478AsnTyr: 0.478 ± 0.213
0.0AsnXaa: 0.0 ± 0.0
Pro
10.038ProAla: 10.038 ± 1.251
0.478ProCys: 0.478 ± 0.232
5.354ProAsp: 5.354 ± 0.865
4.302ProGlu: 4.302 ± 0.669
0.287ProPhe: 0.287 ± 0.161
5.832ProGly: 5.832 ± 0.755
0.765ProHis: 0.765 ± 0.343
1.625ProIle: 1.625 ± 0.37
1.434ProLys: 1.434 ± 0.325
4.493ProLeu: 4.493 ± 0.709
1.434ProMet: 1.434 ± 0.403
0.956ProAsn: 0.956 ± 0.362
4.111ProPro: 4.111 ± 0.866
2.772ProGln: 2.772 ± 0.598
3.633ProArg: 3.633 ± 0.624
4.398ProSer: 4.398 ± 0.6
4.207ProThr: 4.207 ± 1.16
4.111ProVal: 4.111 ± 0.779
0.86ProTrp: 0.86 ± 0.254
1.243ProTyr: 1.243 ± 0.349
0.0ProXaa: 0.0 ± 0.0
Gln
6.023GlnAla: 6.023 ± 0.786
0.191GlnCys: 0.191 ± 0.132
1.434GlnAsp: 1.434 ± 0.349
1.243GlnGlu: 1.243 ± 0.308
1.625GlnPhe: 1.625 ± 0.4
3.25GlnGly: 3.25 ± 0.974
0.956GlnHis: 0.956 ± 0.333
0.765GlnIle: 0.765 ± 0.256
0.574GlnLys: 0.574 ± 0.267
4.302GlnLeu: 4.302 ± 0.614
1.147GlnMet: 1.147 ± 0.313
0.478GlnAsn: 0.478 ± 0.243
2.581GlnPro: 2.581 ± 0.51
3.824GlnGln: 3.824 ± 0.964
4.207GlnArg: 4.207 ± 0.689
1.721GlnSer: 1.721 ± 0.406
2.199GlnThr: 2.199 ± 0.414
2.39GlnVal: 2.39 ± 0.372
1.052GlnTrp: 1.052 ± 0.319
1.338GlnTyr: 1.338 ± 0.283
0.0GlnXaa: 0.0 ± 0.0
Arg
14.532ArgAla: 14.532 ± 1.416
0.478ArgCys: 0.478 ± 0.223
5.067ArgAsp: 5.067 ± 0.804
4.685ArgGlu: 4.685 ± 0.838
1.434ArgPhe: 1.434 ± 0.468
6.023ArgGly: 6.023 ± 0.786
2.677ArgHis: 2.677 ± 0.642
2.964ArgIle: 2.964 ± 0.478
1.816ArgLys: 1.816 ± 0.497
8.509ArgLeu: 8.509 ± 1.012
1.434ArgMet: 1.434 ± 0.339
1.816ArgAsn: 1.816 ± 0.371
5.354ArgPro: 5.354 ± 0.841
3.346ArgGln: 3.346 ± 0.683
8.7ArgArg: 8.7 ± 1.249
4.589ArgSer: 4.589 ± 0.637
7.17ArgThr: 7.17 ± 0.787
6.405ArgVal: 6.405 ± 0.787
1.147ArgTrp: 1.147 ± 0.28
1.912ArgTyr: 1.912 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
6.501SerAla: 6.501 ± 0.887
0.287SerCys: 0.287 ± 0.145
2.39SerAsp: 2.39 ± 0.423
2.008SerGlu: 2.008 ± 0.503
0.765SerPhe: 0.765 ± 0.249
5.545SerGly: 5.545 ± 0.954
0.574SerHis: 0.574 ± 0.232
1.721SerIle: 1.721 ± 0.422
0.765SerLys: 0.765 ± 0.246
4.111SerLeu: 4.111 ± 0.605
1.53SerMet: 1.53 ± 0.473
1.53SerAsn: 1.53 ± 0.33
2.103SerPro: 2.103 ± 0.381
0.86SerGln: 0.86 ± 0.196
4.207SerArg: 4.207 ± 0.572
2.581SerSer: 2.581 ± 0.497
3.728SerThr: 3.728 ± 0.508
2.581SerVal: 2.581 ± 0.431
0.956SerTrp: 0.956 ± 0.276
1.147SerTyr: 1.147 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
13.193ThrAla: 13.193 ± 1.734
0.478ThrCys: 0.478 ± 0.219
3.155ThrAsp: 3.155 ± 0.567
3.059ThrGlu: 3.059 ± 0.386
1.53ThrPhe: 1.53 ± 0.375
6.023ThrGly: 6.023 ± 0.843
0.956ThrHis: 0.956 ± 0.274
3.537ThrIle: 3.537 ± 0.609
2.008ThrLys: 2.008 ± 0.508
6.119ThrLeu: 6.119 ± 0.729
1.052ThrMet: 1.052 ± 0.263
1.53ThrAsn: 1.53 ± 0.441
5.354ThrPro: 5.354 ± 0.785
1.912ThrGln: 1.912 ± 0.389
5.258ThrArg: 5.258 ± 0.667
3.537ThrSer: 3.537 ± 0.537
4.971ThrThr: 4.971 ± 0.671
6.979ThrVal: 6.979 ± 0.826
1.052ThrTrp: 1.052 ± 0.287
1.338ThrTyr: 1.338 ± 0.332
0.0ThrXaa: 0.0 ± 0.0
Val
7.648ValAla: 7.648 ± 0.877
0.287ValCys: 0.287 ± 0.154
5.067ValAsp: 5.067 ± 0.647
5.449ValGlu: 5.449 ± 0.54
0.86ValPhe: 0.86 ± 0.338
4.493ValGly: 4.493 ± 0.766
1.147ValHis: 1.147 ± 0.465
2.677ValIle: 2.677 ± 0.52
1.147ValLys: 1.147 ± 0.313
4.015ValLeu: 4.015 ± 0.692
1.147ValMet: 1.147 ± 0.395
1.434ValAsn: 1.434 ± 0.323
4.78ValPro: 4.78 ± 0.849
1.53ValGln: 1.53 ± 0.372
5.258ValArg: 5.258 ± 0.571
3.346ValSer: 3.346 ± 0.558
5.258ValThr: 5.258 ± 0.666
4.876ValVal: 4.876 ± 0.826
1.434ValTrp: 1.434 ± 0.332
1.53ValTyr: 1.53 ± 0.444
0.0ValXaa: 0.0 ± 0.0
Trp
2.294TrpAla: 2.294 ± 0.454
0.191TrpCys: 0.191 ± 0.125
1.243TrpAsp: 1.243 ± 0.397
0.86TrpGlu: 0.86 ± 0.277
0.191TrpPhe: 0.191 ± 0.126
1.243TrpGly: 1.243 ± 0.261
0.478TrpHis: 0.478 ± 0.244
0.669TrpIle: 0.669 ± 0.274
0.287TrpLys: 0.287 ± 0.151
2.008TrpLeu: 2.008 ± 0.523
0.287TrpMet: 0.287 ± 0.151
0.574TrpAsn: 0.574 ± 0.276
1.052TrpPro: 1.052 ± 0.296
0.765TrpGln: 0.765 ± 0.288
1.434TrpArg: 1.434 ± 0.31
0.86TrpSer: 0.86 ± 0.329
1.912TrpThr: 1.912 ± 0.406
1.147TrpVal: 1.147 ± 0.462
0.382TrpTrp: 0.382 ± 0.168
0.096TrpTyr: 0.096 ± 0.086
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.155TyrAla: 3.155 ± 0.57
0.0TyrCys: 0.0 ± 0.0
2.008TyrAsp: 2.008 ± 0.485
0.86TyrGlu: 0.86 ± 0.289
0.191TyrPhe: 0.191 ± 0.128
2.772TyrGly: 2.772 ± 0.535
0.096TyrHis: 0.096 ± 0.107
0.574TyrIle: 0.574 ± 0.247
0.382TyrLys: 0.382 ± 0.169
1.434TyrLeu: 1.434 ± 0.359
0.191TyrMet: 0.191 ± 0.126
0.287TyrAsn: 0.287 ± 0.155
1.147TyrPro: 1.147 ± 0.336
0.287TyrGln: 0.287 ± 0.16
2.581TyrArg: 2.581 ± 0.5
1.052TyrSer: 1.052 ± 0.331
1.147TyrThr: 1.147 ± 0.333
1.338TyrVal: 1.338 ± 0.348
0.287TyrTrp: 0.287 ± 0.142
0.0TyrTyr: 0.0 ± 0.0
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10461 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski