Enterobacteria phage ES18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5G8S3|Q5G8S3_9CAUD Protein ninE OS=Enterobacteria phage ES18 OX=101570 GN=66 PE=3 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.4PNRR6 pKa = 11.84SYY8 pKa = 10.45EE9 pKa = 3.94QEE11 pKa = 4.07SIARR15 pKa = 11.84AMCAGCNKK23 pKa = 9.44QLAPDD28 pKa = 4.65EE29 pKa = 4.76IYY31 pKa = 11.21VCAEE35 pKa = 4.43CVNEE39 pKa = 3.68WLVYY43 pKa = 10.01RR44 pKa = 11.84DD45 pKa = 3.46QNGDD49 pKa = 3.11MTEE52 pKa = 4.15EE53 pKa = 4.29DD54 pKa = 4.46DD55 pKa = 4.46EE56 pKa = 5.08RR57 pKa = 11.84CIKK60 pKa = 10.6

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5G8S4|Q5G8S4_9CAUD Gp65 OS=Enterobacteria phage ES18 OX=101570 GN=65 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84QRR5 pKa = 11.84RR6 pKa = 11.84SITDD10 pKa = 4.1IICEE14 pKa = 3.96NCKK17 pKa = 10.21YY18 pKa = 10.77LPTKK22 pKa = 10.48RR23 pKa = 11.84SRR25 pKa = 11.84NKK27 pKa = 9.52PKK29 pKa = 10.35PIPTEE34 pKa = 4.16SQVKK38 pKa = 8.12TFDD41 pKa = 3.4YY42 pKa = 11.21VYY44 pKa = 11.4GLLQSKK50 pKa = 7.55WNRR53 pKa = 11.84MRR55 pKa = 11.84RR56 pKa = 11.84TRR58 pKa = 3.62

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14551

26

1124

184.2

20.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.882 ± 0.719

1.354 ± 0.187

5.505 ± 0.208

6.982 ± 0.398

3.12 ± 0.15

6.742 ± 0.333

1.697 ± 0.172

5.842 ± 0.155

6.529 ± 0.375

7.58 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.653 ± 0.128

4.927 ± 0.174

3.649 ± 0.266

4.46 ± 0.353

5.848 ± 0.281

6.405 ± 0.222

5.759 ± 0.408

6.116 ± 0.233

1.697 ± 0.175

3.251 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski