Aeromicrobium sp. Root495

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Aeromicrobium; unclassified Aeromicrobium

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3422 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7QNK8|A0A0Q7QNK8_9ACTN Protoheme IX farnesyltransferase OS=Aeromicrobium sp. Root495 OX=1736550 GN=ctaB PE=3 SV=1
MM1 pKa = 7.39AVVTFVSYY9 pKa = 10.99DD10 pKa = 3.46GEE12 pKa = 4.35KK13 pKa = 10.8FEE15 pKa = 4.6ATLKK19 pKa = 10.42EE20 pKa = 4.54GQSLMQVAVNDD31 pKa = 5.59AIPGIDD37 pKa = 3.66GDD39 pKa = 4.51CGGEE43 pKa = 3.99AACGTCHH50 pKa = 6.63VVVDD54 pKa = 4.42PQWSEE59 pKa = 4.16TVGLSSPEE67 pKa = 3.96EE68 pKa = 3.96EE69 pKa = 5.01EE70 pKa = 4.05MLSMNPEE77 pKa = 3.94RR78 pKa = 11.84QPTSRR83 pKa = 11.84LSCQMAVSAAWDD95 pKa = 3.78GLTVEE100 pKa = 4.99LPEE103 pKa = 4.18FQMM106 pKa = 5.12

Molecular weight: 11.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7QC76|A0A0Q7QC76_9ACTN Adenylyl cyclase class-3/4/guanylyl cyclase OS=Aeromicrobium sp. Root495 OX=1736550 GN=ASD11_16000 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILGDD34 pKa = 3.48RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.86GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3422

0

3422

1067815

37

3034

312.0

33.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.587 ± 0.054

0.62 ± 0.009

6.604 ± 0.037

5.808 ± 0.05

2.871 ± 0.022

8.946 ± 0.04

2.168 ± 0.023

3.596 ± 0.032

2.358 ± 0.036

10.239 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.758 ± 0.015

1.773 ± 0.023

5.268 ± 0.029

2.921 ± 0.022

7.365 ± 0.041

5.832 ± 0.031

6.15 ± 0.038

9.719 ± 0.046

1.491 ± 0.021

1.925 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski