Lake Sarah-associated circular virus-36

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126GAC0|A0A126GAC0_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-36 OX=1685764 PE=3 SV=1
MM1 pKa = 7.38PTPKK5 pKa = 10.05KK6 pKa = 10.53SYY8 pKa = 10.67CFTLNNYY15 pKa = 8.49TDD17 pKa = 4.43GEE19 pKa = 4.43LEE21 pKa = 4.18LLRR24 pKa = 11.84VVCSEE29 pKa = 3.89EE30 pKa = 3.35ASYY33 pKa = 11.41AVIGRR38 pKa = 11.84EE39 pKa = 4.01VGEE42 pKa = 4.37TGTPHH47 pKa = 5.45LQGYY51 pKa = 9.6IRR53 pKa = 11.84FKK55 pKa = 9.77KK56 pKa = 10.08AYY58 pKa = 9.06RR59 pKa = 11.84FSTIKK64 pKa = 10.42DD65 pKa = 3.23RR66 pKa = 11.84YY67 pKa = 10.06LLRR70 pKa = 11.84CHH72 pKa = 7.05IEE74 pKa = 4.14VAAGDD79 pKa = 3.74ADD81 pKa = 4.44SNFRR85 pKa = 11.84YY86 pKa = 9.72CSKK89 pKa = 11.02DD90 pKa = 2.74GDD92 pKa = 3.58FAEE95 pKa = 5.02FGEE98 pKa = 4.89RR99 pKa = 11.84PTSGKK104 pKa = 9.62SASRR108 pKa = 11.84ADD110 pKa = 3.7LARR113 pKa = 11.84SFVSYY118 pKa = 10.39MDD120 pKa = 4.47DD121 pKa = 3.25GRR123 pKa = 11.84SGMVRR128 pKa = 11.84FAHH131 pKa = 6.25EE132 pKa = 4.84NPGTWYY138 pKa = 10.65FSGATMLRR146 pKa = 11.84NYY148 pKa = 10.12YY149 pKa = 10.39ALQPAVDD156 pKa = 3.9RR157 pKa = 11.84SDD159 pKa = 4.88VSVTWIFGDD168 pKa = 3.46PGVGKK173 pKa = 9.98SRR175 pKa = 11.84KK176 pKa = 8.74AHH178 pKa = 5.13EE179 pKa = 4.71TLPQAYY185 pKa = 10.01VKK187 pKa = 10.57DD188 pKa = 4.24PRR190 pKa = 11.84TKK192 pKa = 8.57WWNEE196 pKa = 3.29YY197 pKa = 10.75ALEE200 pKa = 4.48KK201 pKa = 10.92DD202 pKa = 4.18VIIDD206 pKa = 3.57DD207 pKa = 4.82FGPGGIDD214 pKa = 3.15INHH217 pKa = 7.45LLRR220 pKa = 11.84WFDD223 pKa = 3.45RR224 pKa = 11.84YY225 pKa = 10.74KK226 pKa = 11.0CLVEE230 pKa = 4.61SKK232 pKa = 11.01GGMIPLYY239 pKa = 9.99AVNFIVTSNFHH250 pKa = 5.93PEE252 pKa = 3.92KK253 pKa = 10.42VFSFAGDD260 pKa = 3.69PNVQLPALMRR270 pKa = 11.84RR271 pKa = 11.84MIIKK275 pKa = 10.31EE276 pKa = 4.02MVV278 pKa = 2.75

Molecular weight: 31.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126GAC0|A0A126GAC0_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular virus-36 OX=1685764 PE=3 SV=1
MM1 pKa = 7.62AMTGRR6 pKa = 11.84KK7 pKa = 8.47RR8 pKa = 11.84VYY10 pKa = 10.27GGKK13 pKa = 8.17PCKK16 pKa = 10.15NRR18 pKa = 11.84FIKK21 pKa = 9.64KK22 pKa = 8.43RR23 pKa = 11.84RR24 pKa = 11.84MMARR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84SKK33 pKa = 10.63KK34 pKa = 10.22AVGLSTRR41 pKa = 11.84TLAPTDD47 pKa = 3.69FHH49 pKa = 6.63TKK51 pKa = 9.7GRR53 pKa = 11.84MLKK56 pKa = 9.72KK57 pKa = 10.04RR58 pKa = 11.84QWRR61 pKa = 11.84NLLWRR66 pKa = 11.84NTLTSEE72 pKa = 4.48KK73 pKa = 10.16YY74 pKa = 10.18RR75 pKa = 11.84SILMATTGLSTPGNHH90 pKa = 5.45TTANWSDD97 pKa = 3.93LEE99 pKa = 4.31IMSNTQPFWTSAGGMQPVNQGSGVPTLAPDD129 pKa = 4.2HH130 pKa = 6.47IIIRR134 pKa = 11.84GGTFYY139 pKa = 11.16FSMINPLGNTTDD151 pKa = 2.82IRR153 pKa = 11.84VRR155 pKa = 11.84LQLLYY160 pKa = 9.84PKK162 pKa = 10.6QNITRR167 pKa = 11.84YY168 pKa = 9.88ASLVRR173 pKa = 11.84TNVPLIDD180 pKa = 4.63WITSLGGNQPTIWRR194 pKa = 11.84MQDD197 pKa = 2.71AGDD200 pKa = 3.77YY201 pKa = 8.79NQYY204 pKa = 10.65YY205 pKa = 9.18YY206 pKa = 11.29NPVMEE211 pKa = 5.24KK212 pKa = 10.48EE213 pKa = 4.6IILKK217 pKa = 10.26PGDD220 pKa = 3.53SFEE223 pKa = 4.92EE224 pKa = 4.03YY225 pKa = 10.68RR226 pKa = 11.84KK227 pKa = 9.74MKK229 pKa = 9.59VQRR232 pKa = 11.84LDD234 pKa = 3.15TEE236 pKa = 4.36AFLNGAAGFPSWLVYY251 pKa = 10.21ISNLDD256 pKa = 3.24GAAVQNVTVVSGHH269 pKa = 5.55SLSFALADD277 pKa = 3.52VV278 pKa = 4.0

Molecular weight: 31.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

556

278

278

278.0

31.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.655 ± 0.332

1.079 ± 0.443

5.396 ± 0.886

4.137 ± 0.997

4.496 ± 0.776

7.914 ± 0.0

1.799 ± 0.222

5.036 ± 0.222

5.755 ± 0.0

7.554 ± 0.665

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.597 ± 0.443

4.856 ± 0.776

5.036 ± 0.0

2.698 ± 0.776

7.734 ± 0.332

6.655 ± 0.111

6.655 ± 1.219

6.115 ± 0.443

2.158 ± 0.222

4.676 ± 0.443
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski