Tomato leaf curl Pakistan alphasatellite

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; Colecusatellite

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C4PLB1|C4PLB1_9VIRU Replication associated protein OS=Tomato leaf curl Pakistan alphasatellite OX=538004 GN=rep PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.4EE3 pKa = 3.83EE4 pKa = 4.2TRR6 pKa = 11.84VSGPYY11 pKa = 10.23EE12 pKa = 3.83FGNYY16 pKa = 7.28VAGGSNKK23 pKa = 9.78RR24 pKa = 11.84KK25 pKa = 10.47LEE27 pKa = 4.22DD28 pKa = 3.91LLGNSDD34 pKa = 4.07NEE36 pKa = 3.86IEE38 pKa = 4.36EE39 pKa = 4.15PQKK42 pKa = 10.82YY43 pKa = 8.59RR44 pKa = 11.84RR45 pKa = 11.84AMAMKK50 pKa = 7.51MTKK53 pKa = 9.83VSHH56 pKa = 5.59QWALDD61 pKa = 3.42NPFPFEE67 pKa = 3.87LKK69 pKa = 9.38EE70 pKa = 3.9WQEE73 pKa = 4.04RR74 pKa = 11.84LSGDD78 pKa = 4.05LNLNPDD84 pKa = 3.62DD85 pKa = 3.98RR86 pKa = 11.84TIVWVYY92 pKa = 10.79GPTGGEE98 pKa = 4.2GKK100 pKa = 9.71SQFAKK105 pKa = 10.98YY106 pKa = 10.3LGLNKK111 pKa = 10.07NWLYY115 pKa = 11.36LPGGKK120 pKa = 9.89VNDD123 pKa = 3.79MMYY126 pKa = 9.22MYY128 pKa = 10.44CKK130 pKa = 10.15KK131 pKa = 9.83PQSNLVIDD139 pKa = 4.02YY140 pKa = 7.37PRR142 pKa = 11.84CNKK145 pKa = 10.33DD146 pKa = 3.49FINYY150 pKa = 9.49AFLEE154 pKa = 4.39MVKK157 pKa = 10.27NRR159 pKa = 11.84TVYY162 pKa = 9.36SYY164 pKa = 10.87KK165 pKa = 10.28YY166 pKa = 9.17EE167 pKa = 3.95PVGFIDD173 pKa = 3.68PTCNVHH179 pKa = 5.82VVVMANFLPDD189 pKa = 3.54YY190 pKa = 10.72EE191 pKa = 5.58RR192 pKa = 11.84ISQDD196 pKa = 3.2RR197 pKa = 11.84IKK199 pKa = 11.06LIDD202 pKa = 3.71LSS204 pKa = 3.96

Molecular weight: 23.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C4PLB1|C4PLB1_9VIRU Replication associated protein OS=Tomato leaf curl Pakistan alphasatellite OX=538004 GN=rep PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.4EE3 pKa = 3.83EE4 pKa = 4.2TRR6 pKa = 11.84VSGPYY11 pKa = 10.23EE12 pKa = 3.83FGNYY16 pKa = 7.28VAGGSNKK23 pKa = 9.78RR24 pKa = 11.84KK25 pKa = 10.47LEE27 pKa = 4.22DD28 pKa = 3.91LLGNSDD34 pKa = 4.07NEE36 pKa = 3.86IEE38 pKa = 4.36EE39 pKa = 4.15PQKK42 pKa = 10.82YY43 pKa = 8.59RR44 pKa = 11.84RR45 pKa = 11.84AMAMKK50 pKa = 7.51MTKK53 pKa = 9.83VSHH56 pKa = 5.59QWALDD61 pKa = 3.42NPFPFEE67 pKa = 3.87LKK69 pKa = 9.38EE70 pKa = 3.9WQEE73 pKa = 4.04RR74 pKa = 11.84LSGDD78 pKa = 4.05LNLNPDD84 pKa = 3.62DD85 pKa = 3.98RR86 pKa = 11.84TIVWVYY92 pKa = 10.79GPTGGEE98 pKa = 4.2GKK100 pKa = 9.71SQFAKK105 pKa = 10.98YY106 pKa = 10.3LGLNKK111 pKa = 10.07NWLYY115 pKa = 11.36LPGGKK120 pKa = 9.89VNDD123 pKa = 3.79MMYY126 pKa = 9.22MYY128 pKa = 10.44CKK130 pKa = 10.15KK131 pKa = 9.83PQSNLVIDD139 pKa = 4.02YY140 pKa = 7.37PRR142 pKa = 11.84CNKK145 pKa = 10.33DD146 pKa = 3.49FINYY150 pKa = 9.49AFLEE154 pKa = 4.39MVKK157 pKa = 10.27NRR159 pKa = 11.84TVYY162 pKa = 9.36SYY164 pKa = 10.87KK165 pKa = 10.28YY166 pKa = 9.17EE167 pKa = 3.95PVGFIDD173 pKa = 3.68PTCNVHH179 pKa = 5.82VVVMANFLPDD189 pKa = 3.54YY190 pKa = 10.72EE191 pKa = 5.58RR192 pKa = 11.84ISQDD196 pKa = 3.2RR197 pKa = 11.84IKK199 pKa = 11.06LIDD202 pKa = 3.71LSS204 pKa = 3.96

Molecular weight: 23.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

204

204

204

204.0

23.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.431 ± 0.0

1.471 ± 0.0

6.373 ± 0.0

6.863 ± 0.0

3.922 ± 0.0

6.863 ± 0.0

0.98 ± 0.0

3.922 ± 0.0

8.333 ± 0.0

8.333 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.412 ± 0.0

7.843 ± 0.0

5.882 ± 0.0

2.941 ± 0.0

4.902 ± 0.0

4.902 ± 0.0

2.941 ± 0.0

6.863 ± 0.0

1.961 ± 0.0

6.863 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski