Tetradesmus obliquus (Green alga) (Acutodesmus obliquus)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18538 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A383VB91|A0A383VB91_TETOB MIT domain-containing protein OS=Tetradesmus obliquus OX=3088 GN=BQ4739_LOCUS3053 PE=4 SV=1
MM1 pKa = 7.54ALSDD5 pKa = 4.82DD6 pKa = 3.94PHH8 pKa = 9.03ASDD11 pKa = 4.86QEE13 pKa = 4.39SDD15 pKa = 3.9GEE17 pKa = 4.35GLDD20 pKa = 3.55EE21 pKa = 5.66EE22 pKa = 4.89QYY24 pKa = 11.03QWLQEE29 pKa = 4.05QLAHH33 pKa = 6.78IGNLHH38 pKa = 6.26IEE40 pKa = 4.29GDD42 pKa = 3.8EE43 pKa = 5.1EE44 pKa = 4.0EE45 pKa = 5.79DD46 pKa = 3.25EE47 pKa = 4.37WADD50 pKa = 3.45PSGGLLVNIFAACEE64 pKa = 3.9EE65 pKa = 4.38GSMDD69 pKa = 4.65QLTTNIQEE77 pKa = 4.68LKK79 pKa = 9.67DD80 pKa = 3.46TQYY83 pKa = 11.81DD84 pKa = 4.01VNTPGPDD91 pKa = 3.3GDD93 pKa = 4.22TALHH97 pKa = 6.39LACLYY102 pKa = 10.12GHH104 pKa = 5.61QQCAEE109 pKa = 4.25LLLQEE114 pKa = 4.44GAKK117 pKa = 10.16ADD119 pKa = 3.67AVNPEE124 pKa = 4.72DD125 pKa = 4.04GTTPLHH131 pKa = 6.66DD132 pKa = 4.02AAAGGYY138 pKa = 10.05AGIVQMLLEE147 pKa = 4.19KK148 pKa = 10.46SGPGSVSLQDD158 pKa = 3.58SDD160 pKa = 5.31GDD162 pKa = 4.17TALHH166 pKa = 5.44NAARR170 pKa = 11.84GGHH173 pKa = 6.15LAVVQQLLAAGAAPSVLNSSGKK195 pKa = 8.01TPAGEE200 pKa = 4.07SDD202 pKa = 3.34NQEE205 pKa = 3.78VVRR208 pKa = 11.84ALVAAAAAALGVGGTAPAAAAAAPAAAPAADD239 pKa = 3.74TAACGASMDD248 pKa = 3.87AQQ250 pKa = 3.49

Molecular weight: 25.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A383WP31|A0A383WP31_TETOB Uncharacterized protein OS=Tetradesmus obliquus OX=3088 GN=BQ4739_LOCUS19502 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84SHH5 pKa = 7.12VITTLVLIAFAAVVTANVEE24 pKa = 4.0DD25 pKa = 3.86DD26 pKa = 3.59HH27 pKa = 6.44TRR29 pKa = 11.84KK30 pKa = 9.9LLQGNARR37 pKa = 11.84STGAMMSQHH46 pKa = 5.68STSAAIKK53 pKa = 7.41TTVQNAQAGRR63 pKa = 11.84GSTGNTKK70 pKa = 10.3AATRR74 pKa = 11.84TGSVATTVLARR85 pKa = 11.84PSGPNTATAGAVTRR99 pKa = 11.84AATPANRR106 pKa = 11.84PTGRR110 pKa = 11.84HH111 pKa = 5.43LLQARR116 pKa = 11.84NTGAAGSRR124 pKa = 11.84ATASQATTSAAIKK137 pKa = 8.85QTVQAANRR145 pKa = 11.84GAPVAAKK152 pKa = 9.19PATRR156 pKa = 11.84TGQTAAQVIARR167 pKa = 11.84PAGLPATVTSQAVTTRR183 pKa = 11.84PAQQGARR190 pKa = 11.84TGRR193 pKa = 11.84HH194 pKa = 5.36LLQARR199 pKa = 11.84NTGAAGSRR207 pKa = 11.84ATASQATTSAAIKK220 pKa = 8.85QTVQAANRR228 pKa = 11.84GTPVAAKK235 pKa = 9.01PATRR239 pKa = 11.84TGQTAAQVIARR250 pKa = 11.84PAGLPATVTSQAVTTRR266 pKa = 11.84PAQQRR271 pKa = 11.84TGRR274 pKa = 11.84HH275 pKa = 5.18LLQNSRR281 pKa = 11.84TGANQGRR288 pKa = 11.84ATAAQGTTSAAIRR301 pKa = 11.84QTVQAANRR309 pKa = 11.84GAPVAAKK316 pKa = 9.19PATRR320 pKa = 11.84TGQAAAQTIARR331 pKa = 11.84PAGLPATVTSQAVTTRR347 pKa = 11.84PAQQNPRR354 pKa = 11.84GLL356 pKa = 3.62

Molecular weight: 35.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17061

1477

18538

10808351

11

19142

583.0

61.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.312 ± 0.051

1.756 ± 0.011

4.299 ± 0.012

4.558 ± 0.017

2.301 ± 0.009

8.188 ± 0.02

2.148 ± 0.008

2.389 ± 0.012

3.143 ± 0.016

9.543 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.003 ± 0.008

2.307 ± 0.012

5.784 ± 0.02

7.882 ± 0.044

5.114 ± 0.013

8.719 ± 0.03

4.452 ± 0.017

6.007 ± 0.016

1.271 ± 0.006

1.825 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski