Nocardioides sp. CF8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides; unclassified Nocardioides

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3893 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7XWH7|R7XWH7_9ACTN Uncharacterized protein OS=Nocardioides sp. CF8 OX=110319 GN=CF8_2320 PE=4 SV=1
MM1 pKa = 7.56ALSLHH6 pKa = 5.92SRR8 pKa = 11.84LLITAALVTVAATSGGAAYY27 pKa = 10.34ADD29 pKa = 3.11QVANNLDD36 pKa = 3.61GSVDD40 pKa = 3.31AAAEE44 pKa = 4.06VMPLNVGADD53 pKa = 3.4GSTVLRR59 pKa = 11.84VLTANGDD66 pKa = 3.92GKK68 pKa = 10.55NGCNLTGATIATFSVQSSATDD89 pKa = 3.4VATVTPGTVTFDD101 pKa = 3.1SCEE104 pKa = 3.89RR105 pKa = 11.84TVVLTVTAHH114 pKa = 5.68SVGSSAITLTQTANTTSGSFEE135 pKa = 3.91VDD137 pKa = 2.9PASFTVNVAPPANTAPGVEE156 pKa = 4.12VTGIDD161 pKa = 3.19RR162 pKa = 11.84SSYY165 pKa = 10.83EE166 pKa = 4.3LGLDD170 pKa = 3.47TLPTPGCAATDD181 pKa = 3.89AEE183 pKa = 5.29DD184 pKa = 4.88GPSSPEE190 pKa = 3.61PTVLDD195 pKa = 3.49TRR197 pKa = 11.84DD198 pKa = 3.5ALGLGTVSVSCAYY211 pKa = 9.4TDD213 pKa = 3.43EE214 pKa = 5.39GGLSAVSSQSYY225 pKa = 8.16TVVDD229 pKa = 4.85TIAPVIEE236 pKa = 4.47LVSVTPAAGAHH247 pKa = 5.88GWHH250 pKa = 6.95GSPVTATWSCSDD262 pKa = 3.31AGSGVEE268 pKa = 3.81AAEE271 pKa = 4.39VSATTVGEE279 pKa = 4.32GGDD282 pKa = 4.08LEE284 pKa = 4.79LTDD287 pKa = 3.82TCTDD291 pKa = 3.47RR292 pKa = 11.84AGHH295 pKa = 5.26STSDD299 pKa = 3.24TVEE302 pKa = 4.2DD303 pKa = 4.18LRR305 pKa = 11.84VDD307 pKa = 3.47LTDD310 pKa = 3.54PVITIGRR317 pKa = 11.84SPAANAAGWNNVDD330 pKa = 3.54VTVAWACADD339 pKa = 3.98TLSGTTDD346 pKa = 3.38PGGSAVLGEE355 pKa = 4.65GADD358 pKa = 3.55QSVTATCTDD367 pKa = 3.63LAGNSATDD375 pKa = 3.31EE376 pKa = 4.31VTDD379 pKa = 3.64VDD381 pKa = 3.96VDD383 pKa = 3.64QTAPEE388 pKa = 4.24VSWTSPIADD397 pKa = 3.37GSSYY401 pKa = 11.37YY402 pKa = 10.43FGSVPDD408 pKa = 4.57SPTCSATDD416 pKa = 4.03LLSGLDD422 pKa = 3.66GACAVGGHH430 pKa = 6.02ATTVGTHH437 pKa = 5.1TVTARR442 pKa = 11.84ASDD445 pKa = 3.71VAGNTSQLSSSYY457 pKa = 10.9TVMAWTLNGYY467 pKa = 8.19DD468 pKa = 3.95KK469 pKa = 11.0PVDD472 pKa = 4.0GVGVWNTVKK481 pKa = 10.79NGSTVPLKK489 pKa = 10.84FEE491 pKa = 4.48ALMGSAEE498 pKa = 4.12ITDD501 pKa = 3.87VTTLGAQFTVKK512 pKa = 10.22GVACPGAGAVTEE524 pKa = 4.42DD525 pKa = 4.24VEE527 pKa = 4.47LTTTGGTTFRR537 pKa = 11.84YY538 pKa = 9.44DD539 pKa = 2.75WSGGQFVQNWQTPKK553 pKa = 10.7KK554 pKa = 10.5AGACYY559 pKa = 9.75QVITTTADD567 pKa = 3.33GSSLSALFKK576 pKa = 11.11LKK578 pKa = 10.75

Molecular weight: 57.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7XSU3|R7XSU3_9ACTN Integral membrane protein MviN OS=Nocardioides sp. CF8 OX=110319 GN=CF8_3779 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.97KK15 pKa = 10.04VHH17 pKa = 6.59GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.73GRR40 pKa = 11.84SSLAVV45 pKa = 3.16

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3893

0

3893

1249285

31

1978

320.9

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.802 ± 0.049

0.746 ± 0.011

6.589 ± 0.031

5.844 ± 0.036

2.914 ± 0.021

8.973 ± 0.035

2.291 ± 0.02

3.874 ± 0.026

2.226 ± 0.034

10.185 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.97 ± 0.015

1.907 ± 0.022

5.388 ± 0.031

2.811 ± 0.019

7.302 ± 0.04

5.482 ± 0.028

6.221 ± 0.03

9.076 ± 0.037

1.499 ± 0.016

1.901 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski