alpha proteobacterium AAP81b

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3055 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N0JUJ6|A0A0N0JUJ6_9PROT Peptidase_M56 domain-containing protein (Fragment) OS=alpha proteobacterium AAP81b OX=1523432 GN=IP88_11860 PE=4 SV=1
MM1 pKa = 7.55GAQSDD6 pKa = 4.09PNKK9 pKa = 10.23EE10 pKa = 3.68VLYY13 pKa = 11.11YY14 pKa = 10.96GDD16 pKa = 3.7TGEE19 pKa = 5.43LIGTEE24 pKa = 4.34SFSLDD29 pKa = 3.35PFAGGSYY36 pKa = 9.85LGKK39 pKa = 9.77PIADD43 pKa = 3.43LEE45 pKa = 4.5FVTEE49 pKa = 3.91NLVRR53 pKa = 11.84SGSNWLNGANYY64 pKa = 10.08GVLDD68 pKa = 4.75DD69 pKa = 4.7GVLNFGFWNNIQEE82 pKa = 4.47LQNSYY87 pKa = 10.56YY88 pKa = 11.18VNDD91 pKa = 4.48DD92 pKa = 3.03GSIRR96 pKa = 11.84FSEE99 pKa = 4.4ANPATFSAFSTAQRR113 pKa = 11.84AVARR117 pKa = 11.84TTIALWDD124 pKa = 4.06DD125 pKa = 4.81LIDD128 pKa = 3.35ISFRR132 pKa = 11.84EE133 pKa = 4.26TRR135 pKa = 11.84SGVADD140 pKa = 3.11ITYY143 pKa = 10.62GNTAAGANVQAYY155 pKa = 9.85AYY157 pKa = 10.43LPFGDD162 pKa = 5.07VYY164 pKa = 11.54DD165 pKa = 4.85DD166 pKa = 4.59YY167 pKa = 11.54YY168 pKa = 11.83AQFGWEE174 pKa = 3.84TAQIGRR180 pKa = 11.84LGGDD184 pKa = 2.95VWIGGNVGSNFTPLQNSYY202 pKa = 11.05YY203 pKa = 10.86SVITQIHH210 pKa = 4.92EE211 pKa = 4.06TGHH214 pKa = 6.61ALGLSHH220 pKa = 7.5PGDD223 pKa = 4.15YY224 pKa = 10.47NASDD228 pKa = 4.72DD229 pKa = 3.9NDD231 pKa = 5.33GIPGPDD237 pKa = 4.49PITYY241 pKa = 10.03TNDD244 pKa = 2.64AYY246 pKa = 10.67FAQDD250 pKa = 3.09SLQYY254 pKa = 11.3SVMSYY259 pKa = 10.38FDD261 pKa = 3.77AYY263 pKa = 9.06EE264 pKa = 4.12TGAQHH269 pKa = 7.46IDD271 pKa = 2.78WTLTNFAYY279 pKa = 10.31AATPLVHH286 pKa = 7.34DD287 pKa = 4.51VAAIQALYY295 pKa = 10.59GVDD298 pKa = 3.05TTTRR302 pKa = 11.84TGDD305 pKa = 3.16TVYY308 pKa = 11.03GFNSTANRR316 pKa = 11.84TAYY319 pKa = 10.46DD320 pKa = 3.59FTSNTRR326 pKa = 11.84PIVTIWDD333 pKa = 3.32AGGIDD338 pKa = 4.15TIDD341 pKa = 3.43FSGWNTPSVIDD352 pKa = 4.18LNEE355 pKa = 4.09GAFSSGGGTEE365 pKa = 3.93QFLTLAQVNANRR377 pKa = 11.84AALGFAPRR385 pKa = 11.84SQATFNFYY393 pKa = 10.86EE394 pKa = 4.36GLKK397 pKa = 10.35AQLGLTNGLFKK408 pKa = 11.24DD409 pKa = 4.03NVSIAYY415 pKa = 8.71GATIEE420 pKa = 4.11NATGGGGNDD429 pKa = 2.82RR430 pKa = 11.84LIANNVANVLTGNGGVDD447 pKa = 4.1FASYY451 pKa = 9.88EE452 pKa = 3.94AATAGVTVNLATGGTAGYY470 pKa = 9.93AAGDD474 pKa = 3.86SYY476 pKa = 11.99VGIEE480 pKa = 4.14GVIGSDD486 pKa = 3.96FNDD489 pKa = 3.53VLDD492 pKa = 5.03GSAGDD497 pKa = 4.42DD498 pKa = 3.55LFAVSGGADD507 pKa = 4.25AINGGAGSDD516 pKa = 3.19TLTFQFAEE524 pKa = 4.13AGVNVSLTGTGLGGHH539 pKa = 6.25TLTSIEE545 pKa = 4.47NLTGSAFNDD554 pKa = 3.71TLGGDD559 pKa = 3.53SAANVINGGAGIDD572 pKa = 3.45TLFYY576 pKa = 10.94AGATSGVTVNLATGGTRR593 pKa = 11.84GLAAGDD599 pKa = 3.73TYY601 pKa = 11.76VSIEE605 pKa = 4.65NITATNFADD614 pKa = 4.41NLTGDD619 pKa = 3.48AGVNVIQGGLGADD632 pKa = 3.82TLTGGAGDD640 pKa = 3.78VLSYY644 pKa = 11.14ADD646 pKa = 3.46SAAAITINLGNNYY659 pKa = 9.95AAGGTAARR667 pKa = 11.84DD668 pKa = 3.83VISGFDD674 pKa = 3.42NVTASAFNDD683 pKa = 3.99NITGDD688 pKa = 3.59NDD690 pKa = 3.99ANTLTGGDD698 pKa = 4.17GNDD701 pKa = 3.75TLIGAAGADD710 pKa = 3.48QLFGGIGNDD719 pKa = 4.15SIRR722 pKa = 11.84AGNDD726 pKa = 2.42NDD728 pKa = 4.07YY729 pKa = 11.35IEE731 pKa = 5.33AGNGNDD737 pKa = 3.7TVLGDD742 pKa = 4.06AQNDD746 pKa = 4.12TLFGGAGDD754 pKa = 4.66DD755 pKa = 3.86NLNGGGNDD763 pKa = 3.79DD764 pKa = 4.28YY765 pKa = 11.9LNGGTGTNTLTGGTGLDD782 pKa = 3.11IFDD785 pKa = 5.29FDD787 pKa = 4.19TASFTDD793 pKa = 4.43TITDD797 pKa = 3.94FRR799 pKa = 11.84TGLDD803 pKa = 3.86KK804 pKa = 10.74IDD806 pKa = 3.65VSDD809 pKa = 3.31IAGFTFIGGAAFSGANQLRR828 pKa = 11.84FYY830 pKa = 10.9TDD832 pKa = 3.1GANRR836 pKa = 11.84ALAGDD841 pKa = 3.54IDD843 pKa = 4.67GDD845 pKa = 4.28GVADD849 pKa = 4.48LVIHH853 pKa = 5.92LTGAVSVVAGDD864 pKa = 4.79LILVV868 pKa = 4.17

Molecular weight: 89.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N0K463|A0A0N0K463_9PROT Uncharacterized protein OS=alpha proteobacterium AAP81b OX=1523432 GN=IP88_02650 PE=4 SV=1
MM1 pKa = 6.79TAAPRR6 pKa = 11.84TIGRR10 pKa = 11.84PPRR13 pKa = 11.84RR14 pKa = 11.84QARR17 pKa = 11.84GRR19 pKa = 11.84QAIAALALLCGLALAGADD37 pKa = 3.62PAAAQPSGPWKK48 pKa = 10.5GDD50 pKa = 3.01PCRR53 pKa = 11.84YY54 pKa = 9.13FRR56 pKa = 11.84QQRR59 pKa = 11.84AAGRR63 pKa = 11.84CTGVGNWKK71 pKa = 10.33VNEE74 pKa = 4.2GDD76 pKa = 4.06SFTTYY81 pKa = 10.38HH82 pKa = 7.1ADD84 pKa = 3.08GTLRR88 pKa = 11.84SNTPPPPPPPPLPPLPPPSPDD109 pKa = 2.68WVPPEE114 pKa = 4.19GFEE117 pKa = 4.29PPRR120 pKa = 11.84EE121 pKa = 4.2AAVTAPMPAATAVGAVSPGPVSSGPWRR148 pKa = 11.84GDD150 pKa = 3.02PCGHH154 pKa = 6.35FRR156 pKa = 11.84RR157 pKa = 11.84GNVSGRR163 pKa = 11.84CDD165 pKa = 3.12KK166 pKa = 11.23GVWKK170 pKa = 10.21QATGADD176 pKa = 3.33RR177 pKa = 11.84FQWFFADD184 pKa = 4.33GSSRR188 pKa = 11.84TVTSAPAVNNSKK200 pKa = 9.11TMTLTIAALPPAVRR214 pKa = 11.84VEE216 pKa = 4.07ATIAPPDD223 pKa = 4.16PPSGAWRR230 pKa = 11.84GDD232 pKa = 3.0PCGYY236 pKa = 9.73FRR238 pKa = 11.84RR239 pKa = 11.84SNAVGRR245 pKa = 11.84CQNGSWRR252 pKa = 11.84IAAGTGRR259 pKa = 11.84LTLFLADD266 pKa = 3.73GSSRR270 pKa = 11.84TITVQPALPATKK282 pKa = 10.28

Molecular weight: 29.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3055

0

3055

934311

41

5206

305.8

32.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.746 ± 0.082

0.696 ± 0.014

5.94 ± 0.035

4.616 ± 0.043

3.496 ± 0.027

9.493 ± 0.074

1.814 ± 0.027

4.65 ± 0.027

2.391 ± 0.037

10.31 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.053 ± 0.026

2.355 ± 0.053

5.587 ± 0.048

2.615 ± 0.023

7.497 ± 0.057

4.509 ± 0.037

5.398 ± 0.071

7.453 ± 0.037

1.432 ± 0.022

1.95 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski