Okra enation leaf curl alphasatellite

Taxonomy: Viruses; Alphasatellitidae; Geminialphasatellitinae; Gosmusatellite

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0R684|L0R684_9VIRU Replication associated protein OS=Okra enation leaf curl alphasatellite OX=1249467 GN=Rep PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84APSADD7 pKa = 3.3TARR10 pKa = 11.84DD11 pKa = 3.66YY12 pKa = 11.22CSKK15 pKa = 11.25DD16 pKa = 3.22EE17 pKa = 4.3TRR19 pKa = 11.84VSGPWVFGTFVPMGSNKK36 pKa = 9.83RR37 pKa = 11.84KK38 pKa = 8.79LTEE41 pKa = 4.51LLDD44 pKa = 3.95NSDD47 pKa = 4.12NEE49 pKa = 3.97IEE51 pKa = 4.35EE52 pKa = 4.15PQKK55 pKa = 10.82YY56 pKa = 8.59RR57 pKa = 11.84RR58 pKa = 11.84AMAMKK63 pKa = 7.86MTKK66 pKa = 9.89EE67 pKa = 3.86SHH69 pKa = 4.56QWALEE74 pKa = 3.93NPFPFEE80 pKa = 3.77LKK82 pKa = 9.43EE83 pKa = 3.88WQEE86 pKa = 3.97RR87 pKa = 11.84LLSDD91 pKa = 4.98FNLNPDD97 pKa = 3.58DD98 pKa = 4.41RR99 pKa = 11.84TIFWVYY105 pKa = 10.74GPTGGEE111 pKa = 4.2GKK113 pKa = 9.71SQFAKK118 pKa = 10.98YY119 pKa = 10.3LGLNKK124 pKa = 10.07NWLYY128 pKa = 11.36LPGGKK133 pKa = 9.89VNDD136 pKa = 3.79MMYY139 pKa = 9.15MYY141 pKa = 10.66CKK143 pKa = 9.48TPRR146 pKa = 11.84RR147 pKa = 11.84NLVIDD152 pKa = 3.58YY153 pKa = 7.33PRR155 pKa = 11.84CNKK158 pKa = 10.33DD159 pKa = 3.49FINYY163 pKa = 9.49AFLEE167 pKa = 4.39MVKK170 pKa = 10.27NRR172 pKa = 11.84TVYY175 pKa = 9.36SYY177 pKa = 10.87KK178 pKa = 10.28YY179 pKa = 9.17EE180 pKa = 3.95PVGFIDD186 pKa = 3.68PTCNVHH192 pKa = 5.82VVVMANFLPDD202 pKa = 3.39YY203 pKa = 10.64EE204 pKa = 5.29RR205 pKa = 11.84ISEE208 pKa = 4.16DD209 pKa = 3.58RR210 pKa = 11.84IKK212 pKa = 11.11LIDD215 pKa = 3.78LSS217 pKa = 3.96

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0R684|L0R684_9VIRU Replication associated protein OS=Okra enation leaf curl alphasatellite OX=1249467 GN=Rep PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84APSADD7 pKa = 3.3TARR10 pKa = 11.84DD11 pKa = 3.66YY12 pKa = 11.22CSKK15 pKa = 11.25DD16 pKa = 3.22EE17 pKa = 4.3TRR19 pKa = 11.84VSGPWVFGTFVPMGSNKK36 pKa = 9.83RR37 pKa = 11.84KK38 pKa = 8.79LTEE41 pKa = 4.51LLDD44 pKa = 3.95NSDD47 pKa = 4.12NEE49 pKa = 3.97IEE51 pKa = 4.35EE52 pKa = 4.15PQKK55 pKa = 10.82YY56 pKa = 8.59RR57 pKa = 11.84RR58 pKa = 11.84AMAMKK63 pKa = 7.86MTKK66 pKa = 9.89EE67 pKa = 3.86SHH69 pKa = 4.56QWALEE74 pKa = 3.93NPFPFEE80 pKa = 3.77LKK82 pKa = 9.43EE83 pKa = 3.88WQEE86 pKa = 3.97RR87 pKa = 11.84LLSDD91 pKa = 4.98FNLNPDD97 pKa = 3.58DD98 pKa = 4.41RR99 pKa = 11.84TIFWVYY105 pKa = 10.74GPTGGEE111 pKa = 4.2GKK113 pKa = 9.71SQFAKK118 pKa = 10.98YY119 pKa = 10.3LGLNKK124 pKa = 10.07NWLYY128 pKa = 11.36LPGGKK133 pKa = 9.89VNDD136 pKa = 3.79MMYY139 pKa = 9.15MYY141 pKa = 10.66CKK143 pKa = 9.48TPRR146 pKa = 11.84RR147 pKa = 11.84NLVIDD152 pKa = 3.58YY153 pKa = 7.33PRR155 pKa = 11.84CNKK158 pKa = 10.33DD159 pKa = 3.49FINYY163 pKa = 9.49AFLEE167 pKa = 4.39MVKK170 pKa = 10.27NRR172 pKa = 11.84TVYY175 pKa = 9.36SYY177 pKa = 10.87KK178 pKa = 10.28YY179 pKa = 9.17EE180 pKa = 3.95PVGFIDD186 pKa = 3.68PTCNVHH192 pKa = 5.82VVVMANFLPDD202 pKa = 3.39YY203 pKa = 10.64EE204 pKa = 5.29RR205 pKa = 11.84ISEE208 pKa = 4.16DD209 pKa = 3.58RR210 pKa = 11.84IKK212 pKa = 11.11LIDD215 pKa = 3.78LSS217 pKa = 3.96

Molecular weight: 25.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

217

217

217

217.0

25.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.147 ± 0.0

1.843 ± 0.0

6.912 ± 0.0

6.912 ± 0.0

5.069 ± 0.0

5.069 ± 0.0

0.922 ± 0.0

3.687 ± 0.0

7.373 ± 0.0

7.834 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.608 ± 0.0

6.912 ± 0.0

6.452 ± 0.0

1.843 ± 0.0

6.452 ± 0.0

5.069 ± 0.0

4.608 ± 0.0

5.991 ± 0.0

2.304 ± 0.0

5.991 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski