Amino acid dipepetide frequency for Salmonella phage BP12C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.299AlaAla: 12.299 ± 1.209
0.837AlaCys: 0.837 ± 0.229
6.856AlaAsp: 6.856 ± 0.558
6.751AlaGlu: 6.751 ± 0.666
3.14AlaPhe: 3.14 ± 0.356
7.746AlaGly: 7.746 ± 0.632
1.413AlaHis: 1.413 ± 0.327
5.234AlaIle: 5.234 ± 0.549
5.705AlaLys: 5.705 ± 0.689
7.537AlaLeu: 7.537 ± 0.882
3.873AlaMet: 3.873 ± 0.534
3.611AlaAsn: 3.611 ± 0.458
3.193AlaPro: 3.193 ± 0.419
3.821AlaGln: 3.821 ± 0.621
5.495AlaArg: 5.495 ± 0.77
4.92AlaSer: 4.92 ± 0.53
5.443AlaThr: 5.443 ± 0.74
6.647AlaVal: 6.647 ± 0.707
1.047AlaTrp: 1.047 ± 0.212
2.983AlaTyr: 2.983 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
0.68CysAla: 0.68 ± 0.244
0.157CysCys: 0.157 ± 0.084
0.994CysAsp: 0.994 ± 0.259
0.733CysGlu: 0.733 ± 0.206
0.262CysPhe: 0.262 ± 0.121
0.68CysGly: 0.68 ± 0.17
0.314CysHis: 0.314 ± 0.116
0.419CysIle: 0.419 ± 0.127
0.523CysLys: 0.523 ± 0.165
0.785CysLeu: 0.785 ± 0.199
0.105CysMet: 0.105 ± 0.063
0.262CysAsn: 0.262 ± 0.124
0.733CysPro: 0.733 ± 0.229
0.209CysGln: 0.209 ± 0.092
0.628CysArg: 0.628 ± 0.257
0.523CysSer: 0.523 ± 0.163
0.628CysThr: 0.628 ± 0.174
0.733CysVal: 0.733 ± 0.175
0.157CysTrp: 0.157 ± 0.086
0.314CysTyr: 0.314 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
6.751AspAla: 6.751 ± 0.479
0.419AspCys: 0.419 ± 0.16
5.181AspAsp: 5.181 ± 0.527
5.705AspGlu: 5.705 ± 0.695
2.617AspPhe: 2.617 ± 0.367
5.652AspGly: 5.652 ± 0.582
1.099AspHis: 1.099 ± 0.266
3.245AspIle: 3.245 ± 0.358
3.297AspLys: 3.297 ± 0.471
5.234AspLeu: 5.234 ± 0.602
2.303AspMet: 2.303 ± 0.347
2.146AspAsn: 2.146 ± 0.365
3.768AspPro: 3.768 ± 0.522
2.146AspGln: 2.146 ± 0.387
3.664AspArg: 3.664 ± 0.425
2.146AspSer: 2.146 ± 0.325
3.716AspThr: 3.716 ± 0.502
4.972AspVal: 4.972 ± 0.457
2.093AspTrp: 2.093 ± 0.327
1.884AspTyr: 1.884 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
5.809GluAla: 5.809 ± 0.632
0.471GluCys: 0.471 ± 0.163
4.187GluAsp: 4.187 ± 0.538
3.821GluGlu: 3.821 ± 0.442
2.355GluPhe: 2.355 ± 0.418
4.03GluGly: 4.03 ± 0.499
1.151GluHis: 1.151 ± 0.283
3.978GluIle: 3.978 ± 0.562
2.931GluLys: 2.931 ± 0.408
4.135GluLeu: 4.135 ± 0.376
1.989GluMet: 1.989 ± 0.33
3.14GluAsn: 3.14 ± 0.515
2.565GluPro: 2.565 ± 0.491
2.617GluGln: 2.617 ± 0.429
3.611GluArg: 3.611 ± 0.549
3.402GluSer: 3.402 ± 0.399
2.983GluThr: 2.983 ± 0.39
4.972GluVal: 4.972 ± 0.522
1.413GluTrp: 1.413 ± 0.214
2.46GluTyr: 2.46 ± 0.4
0.0GluXaa: 0.0 ± 0.0
Phe
3.507PheAla: 3.507 ± 0.486
0.523PheCys: 0.523 ± 0.152
3.193PheAsp: 3.193 ± 0.43
1.832PheGlu: 1.832 ± 0.345
1.204PhePhe: 1.204 ± 0.263
3.14PheGly: 3.14 ± 0.475
0.419PheHis: 0.419 ± 0.148
2.303PheIle: 2.303 ± 0.293
1.936PheLys: 1.936 ± 0.4
2.041PheLeu: 2.041 ± 0.328
0.68PheMet: 0.68 ± 0.2
1.936PheAsn: 1.936 ± 0.332
1.204PhePro: 1.204 ± 0.243
1.204PheGln: 1.204 ± 0.202
2.774PheArg: 2.774 ± 0.363
1.779PheSer: 1.779 ± 0.224
2.146PheThr: 2.146 ± 0.347
1.779PheVal: 1.779 ± 0.279
0.262PheTrp: 0.262 ± 0.15
0.837PheTyr: 0.837 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
6.699GlyAla: 6.699 ± 0.664
1.151GlyCys: 1.151 ± 0.291
4.239GlyAsp: 4.239 ± 0.442
4.92GlyGlu: 4.92 ± 0.411
2.25GlyPhe: 2.25 ± 0.354
6.28GlyGly: 6.28 ± 0.69
1.099GlyHis: 1.099 ± 0.29
3.978GlyIle: 3.978 ± 0.446
3.297GlyLys: 3.297 ± 0.481
5.862GlyLeu: 5.862 ± 0.585
1.518GlyMet: 1.518 ± 0.316
2.512GlyAsn: 2.512 ± 0.378
1.675GlyPro: 1.675 ± 0.321
2.931GlyGln: 2.931 ± 0.522
4.292GlyArg: 4.292 ± 0.494
3.35GlySer: 3.35 ± 0.398
5.024GlyThr: 5.024 ± 0.77
5.077GlyVal: 5.077 ± 0.388
1.675GlyTrp: 1.675 ± 0.303
3.611GlyTyr: 3.611 ± 0.516
0.0GlyXaa: 0.0 ± 0.0
His
1.361HisAla: 1.361 ± 0.285
0.314HisCys: 0.314 ± 0.16
1.518HisAsp: 1.518 ± 0.309
0.994HisGlu: 0.994 ± 0.232
0.68HisPhe: 0.68 ± 0.226
1.204HisGly: 1.204 ± 0.191
0.262HisHis: 0.262 ± 0.146
1.099HisIle: 1.099 ± 0.23
0.68HisLys: 0.68 ± 0.227
1.518HisLeu: 1.518 ± 0.307
0.419HisMet: 0.419 ± 0.157
0.837HisAsn: 0.837 ± 0.192
0.942HisPro: 0.942 ± 0.238
0.419HisGln: 0.419 ± 0.154
1.256HisArg: 1.256 ± 0.245
0.523HisSer: 0.523 ± 0.166
0.523HisThr: 0.523 ± 0.154
0.994HisVal: 0.994 ± 0.295
0.419HisTrp: 0.419 ± 0.16
0.733HisTyr: 0.733 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
5.495IleAla: 5.495 ± 0.628
0.523IleCys: 0.523 ± 0.138
4.187IleAsp: 4.187 ± 0.372
4.239IleGlu: 4.239 ± 0.438
1.622IlePhe: 1.622 ± 0.302
4.292IleGly: 4.292 ± 0.497
1.151IleHis: 1.151 ± 0.263
3.088IleIle: 3.088 ± 0.414
3.14IleLys: 3.14 ± 0.536
3.036IleLeu: 3.036 ± 0.347
1.518IleMet: 1.518 ± 0.292
2.931IleAsn: 2.931 ± 0.421
2.355IlePro: 2.355 ± 0.342
1.675IleGln: 1.675 ± 0.343
3.402IleArg: 3.402 ± 0.423
3.088IleSer: 3.088 ± 0.452
3.297IleThr: 3.297 ± 0.391
4.553IleVal: 4.553 ± 0.527
0.628IleTrp: 0.628 ± 0.199
1.622IleTyr: 1.622 ± 0.325
0.0IleXaa: 0.0 ± 0.0
Lys
4.815LysAla: 4.815 ± 0.553
0.419LysCys: 0.419 ± 0.142
3.297LysAsp: 3.297 ± 0.501
3.245LysGlu: 3.245 ± 0.485
1.518LysPhe: 1.518 ± 0.229
3.297LysGly: 3.297 ± 0.553
0.994LysHis: 0.994 ± 0.221
2.931LysIle: 2.931 ± 0.335
3.14LysLys: 3.14 ± 0.5
3.559LysLeu: 3.559 ± 0.415
1.832LysMet: 1.832 ± 0.306
1.884LysAsn: 1.884 ± 0.366
2.617LysPro: 2.617 ± 0.448
1.675LysGln: 1.675 ± 0.262
3.507LysArg: 3.507 ± 0.455
2.25LysSer: 2.25 ± 0.305
3.768LysThr: 3.768 ± 0.495
3.35LysVal: 3.35 ± 0.299
1.204LysTrp: 1.204 ± 0.291
2.303LysTyr: 2.303 ± 0.321
0.0LysXaa: 0.0 ± 0.0
Leu
7.013LeuAla: 7.013 ± 0.845
0.89LeuCys: 0.89 ± 0.212
4.92LeuAsp: 4.92 ± 0.462
4.449LeuGlu: 4.449 ± 0.428
2.355LeuPhe: 2.355 ± 0.379
4.03LeuGly: 4.03 ± 0.527
1.256LeuHis: 1.256 ± 0.273
4.03LeuIle: 4.03 ± 0.429
4.082LeuLys: 4.082 ± 0.421
4.71LeuLeu: 4.71 ± 0.51
1.832LeuMet: 1.832 ± 0.412
4.03LeuAsn: 4.03 ± 0.446
4.03LeuPro: 4.03 ± 0.625
2.407LeuGln: 2.407 ± 0.47
4.658LeuArg: 4.658 ± 0.517
5.181LeuSer: 5.181 ± 0.704
4.92LeuThr: 4.92 ± 0.44
4.396LeuVal: 4.396 ± 0.486
1.099LeuTrp: 1.099 ± 0.214
2.407LeuTyr: 2.407 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
3.35MetAla: 3.35 ± 0.453
0.366MetCys: 0.366 ± 0.129
1.465MetAsp: 1.465 ± 0.236
1.308MetGlu: 1.308 ± 0.261
0.942MetPhe: 0.942 ± 0.192
1.675MetGly: 1.675 ± 0.324
0.419MetHis: 0.419 ± 0.146
1.465MetIle: 1.465 ± 0.296
2.041MetLys: 2.041 ± 0.324
2.041MetLeu: 2.041 ± 0.273
0.733MetMet: 0.733 ± 0.156
1.518MetAsn: 1.518 ± 0.255
1.047MetPro: 1.047 ± 0.241
1.151MetGln: 1.151 ± 0.305
2.25MetArg: 2.25 ± 0.344
2.198MetSer: 2.198 ± 0.367
2.565MetThr: 2.565 ± 0.338
2.355MetVal: 2.355 ± 0.358
0.471MetTrp: 0.471 ± 0.152
1.308MetTyr: 1.308 ± 0.215
0.0MetXaa: 0.0 ± 0.0
Asn
4.606AsnAla: 4.606 ± 0.553
0.314AsnCys: 0.314 ± 0.121
2.46AsnAsp: 2.46 ± 0.335
1.622AsnGlu: 1.622 ± 0.279
1.465AsnPhe: 1.465 ± 0.313
3.873AsnGly: 3.873 ± 0.406
0.837AsnHis: 0.837 ± 0.248
2.46AsnIle: 2.46 ± 0.389
1.727AsnLys: 1.727 ± 0.319
2.826AsnLeu: 2.826 ± 0.449
1.047AsnMet: 1.047 ± 0.243
1.936AsnAsn: 1.936 ± 0.424
3.454AsnPro: 3.454 ± 0.485
1.989AsnGln: 1.989 ± 0.382
1.727AsnArg: 1.727 ± 0.233
2.774AsnSer: 2.774 ± 0.482
2.512AsnThr: 2.512 ± 0.412
3.088AsnVal: 3.088 ± 0.352
0.471AsnTrp: 0.471 ± 0.151
1.622AsnTyr: 1.622 ± 0.333
0.0AsnXaa: 0.0 ± 0.0
Pro
3.978ProAla: 3.978 ± 0.488
0.523ProCys: 0.523 ± 0.178
3.873ProAsp: 3.873 ± 0.662
3.088ProGlu: 3.088 ± 0.452
1.57ProPhe: 1.57 ± 0.322
3.193ProGly: 3.193 ± 0.536
0.994ProHis: 0.994 ± 0.259
2.512ProIle: 2.512 ± 0.406
2.565ProLys: 2.565 ± 0.389
3.507ProLeu: 3.507 ± 0.582
1.308ProMet: 1.308 ± 0.304
1.989ProAsn: 1.989 ± 0.26
2.565ProPro: 2.565 ± 0.739
1.361ProGln: 1.361 ± 0.304
1.779ProArg: 1.779 ± 0.35
2.774ProSer: 2.774 ± 0.523
2.983ProThr: 2.983 ± 0.453
3.193ProVal: 3.193 ± 0.512
0.628ProTrp: 0.628 ± 0.254
1.989ProTyr: 1.989 ± 0.41
0.0ProXaa: 0.0 ± 0.0
Gln
3.925GlnAla: 3.925 ± 0.663
0.314GlnCys: 0.314 ± 0.121
2.198GlnAsp: 2.198 ± 0.341
1.779GlnGlu: 1.779 ± 0.313
2.198GlnPhe: 2.198 ± 0.369
1.622GlnGly: 1.622 ± 0.314
0.733GlnHis: 0.733 ± 0.274
2.722GlnIle: 2.722 ± 0.296
2.146GlnLys: 2.146 ± 0.389
2.146GlnLeu: 2.146 ± 0.45
1.622GlnMet: 1.622 ± 0.331
1.675GlnAsn: 1.675 ± 0.377
1.675GlnPro: 1.675 ± 0.449
2.041GlnGln: 2.041 ± 0.555
2.407GlnArg: 2.407 ± 0.342
2.25GlnSer: 2.25 ± 0.385
2.093GlnThr: 2.093 ± 0.331
2.879GlnVal: 2.879 ± 0.491
0.576GlnTrp: 0.576 ± 0.158
1.361GlnTyr: 1.361 ± 0.217
0.0GlnXaa: 0.0 ± 0.0
Arg
6.123ArgAla: 6.123 ± 0.679
0.523ArgCys: 0.523 ± 0.153
4.135ArgAsp: 4.135 ± 0.508
3.297ArgGlu: 3.297 ± 0.547
2.617ArgPhe: 2.617 ± 0.398
3.402ArgGly: 3.402 ± 0.406
1.308ArgHis: 1.308 ± 0.296
3.402ArgIle: 3.402 ± 0.364
3.088ArgLys: 3.088 ± 0.4
5.286ArgLeu: 5.286 ± 0.586
2.093ArgMet: 2.093 ± 0.361
2.198ArgAsn: 2.198 ± 0.411
2.617ArgPro: 2.617 ± 0.322
2.198ArgGln: 2.198 ± 0.401
3.611ArgArg: 3.611 ± 0.471
2.617ArgSer: 2.617 ± 0.323
3.454ArgThr: 3.454 ± 0.373
4.867ArgVal: 4.867 ± 0.453
1.099ArgTrp: 1.099 ± 0.228
1.675ArgTyr: 1.675 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
4.815SerAla: 4.815 ± 0.517
0.471SerCys: 0.471 ± 0.135
4.03SerAsp: 4.03 ± 0.449
2.565SerGlu: 2.565 ± 0.356
2.093SerPhe: 2.093 ± 0.323
4.815SerGly: 4.815 ± 0.53
0.628SerHis: 0.628 ± 0.143
2.879SerIle: 2.879 ± 0.329
2.669SerLys: 2.669 ± 0.415
3.978SerLeu: 3.978 ± 0.519
1.727SerMet: 1.727 ± 0.296
2.46SerAsn: 2.46 ± 0.295
2.669SerPro: 2.669 ± 0.292
2.565SerGln: 2.565 ± 0.494
2.826SerArg: 2.826 ± 0.466
2.983SerSer: 2.983 ± 0.662
3.14SerThr: 3.14 ± 0.466
4.239SerVal: 4.239 ± 0.372
0.942SerTrp: 0.942 ± 0.233
1.675SerTyr: 1.675 ± 0.412
0.0SerXaa: 0.0 ± 0.0
Thr
6.176ThrAla: 6.176 ± 0.547
0.366ThrCys: 0.366 ± 0.144
3.664ThrAsp: 3.664 ± 0.425
2.931ThrGlu: 2.931 ± 0.37
2.041ThrPhe: 2.041 ± 0.373
4.135ThrGly: 4.135 ± 0.532
0.733ThrHis: 0.733 ± 0.222
3.454ThrIle: 3.454 ± 0.49
3.14ThrLys: 3.14 ± 0.429
5.809ThrLeu: 5.809 ± 0.502
1.727ThrMet: 1.727 ± 0.337
2.512ThrAsn: 2.512 ± 0.414
3.559ThrPro: 3.559 ± 0.465
2.617ThrGln: 2.617 ± 0.38
3.35ThrArg: 3.35 ± 0.434
3.35ThrSer: 3.35 ± 0.45
4.867ThrThr: 4.867 ± 0.857
4.71ThrVal: 4.71 ± 0.601
1.308ThrTrp: 1.308 ± 0.239
2.146ThrTyr: 2.146 ± 0.432
0.0ThrXaa: 0.0 ± 0.0
Val
6.333ValAla: 6.333 ± 0.603
0.68ValCys: 0.68 ± 0.201
3.821ValAsp: 3.821 ± 0.376
5.181ValGlu: 5.181 ± 0.424
2.25ValPhe: 2.25 ± 0.379
4.606ValGly: 4.606 ± 0.394
0.837ValHis: 0.837 ± 0.166
4.135ValIle: 4.135 ± 0.488
3.507ValLys: 3.507 ± 0.449
5.129ValLeu: 5.129 ± 0.605
2.407ValMet: 2.407 ± 0.395
3.088ValAsn: 3.088 ± 0.339
2.983ValPro: 2.983 ± 0.44
2.826ValGln: 2.826 ± 0.434
4.92ValArg: 4.92 ± 0.546
4.396ValSer: 4.396 ± 0.486
5.286ValThr: 5.286 ± 0.58
4.92ValVal: 4.92 ± 0.5
1.256ValTrp: 1.256 ± 0.257
2.617ValTyr: 2.617 ± 0.363
0.0ValXaa: 0.0 ± 0.0
Trp
1.465TrpAla: 1.465 ± 0.291
0.157TrpCys: 0.157 ± 0.084
1.779TrpAsp: 1.779 ± 0.336
1.256TrpGlu: 1.256 ± 0.204
0.733TrpPhe: 0.733 ± 0.208
1.256TrpGly: 1.256 ± 0.239
0.366TrpHis: 0.366 ± 0.146
0.837TrpIle: 0.837 ± 0.178
0.68TrpLys: 0.68 ± 0.132
1.465TrpLeu: 1.465 ± 0.272
0.628TrpMet: 0.628 ± 0.161
0.994TrpAsn: 0.994 ± 0.246
0.628TrpPro: 0.628 ± 0.185
0.89TrpGln: 0.89 ± 0.193
0.89TrpArg: 0.89 ± 0.225
1.151TrpSer: 1.151 ± 0.27
0.785TrpThr: 0.785 ± 0.278
0.785TrpVal: 0.785 ± 0.23
0.366TrpTrp: 0.366 ± 0.122
0.523TrpTyr: 0.523 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.35TyrAla: 3.35 ± 0.495
0.419TyrCys: 0.419 ± 0.17
2.041TyrAsp: 2.041 ± 0.279
2.303TyrGlu: 2.303 ± 0.421
0.785TyrPhe: 0.785 ± 0.248
2.512TyrGly: 2.512 ± 0.438
0.68TyrHis: 0.68 ± 0.18
1.675TyrIle: 1.675 ± 0.331
1.308TyrLys: 1.308 ± 0.265
2.093TyrLeu: 2.093 ± 0.453
1.256TyrMet: 1.256 ± 0.225
1.204TyrAsn: 1.204 ± 0.242
2.093TyrPro: 2.093 ± 0.396
1.622TyrGln: 1.622 ± 0.347
2.565TyrArg: 2.565 ± 0.392
2.512TyrSer: 2.512 ± 0.378
2.46TyrThr: 2.46 ± 0.446
2.669TyrVal: 2.669 ± 0.373
0.523TyrTrp: 0.523 ± 0.154
1.099TyrTyr: 1.099 ± 0.243
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (19108 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski