Methanosphaera sp. rholeuAM74

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera; unclassified Methanosphaera

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A328SIU9|A0A328SIU9_9EURY 50S ribosomal protein L24e OS=Methanosphaera sp. rholeuAM74 OX=1945579 GN=rpl24e PE=3 SV=1
MM1 pKa = 7.52NGRR4 pKa = 11.84MHH6 pKa = 7.56DD7 pKa = 3.91KK8 pKa = 10.57NIKK11 pKa = 9.33TMFLLTVLFILLVSATSISASNNTTTDD38 pKa = 3.23DD39 pKa = 4.02AMNNTNVSTSIPIKK53 pKa = 10.72SNVSMDD59 pKa = 3.28SKK61 pKa = 11.67ANQTNNTKK69 pKa = 10.31IIANTSNHH77 pKa = 5.96SNLSKK82 pKa = 9.82TEE84 pKa = 4.04RR85 pKa = 11.84TVKK88 pKa = 10.28SYY90 pKa = 11.1PDD92 pKa = 3.47YY93 pKa = 11.28DD94 pKa = 3.52PYY96 pKa = 11.58GNKK99 pKa = 9.58EE100 pKa = 3.97VLISFTDD107 pKa = 3.13IFDD110 pKa = 4.0GDD112 pKa = 3.79VIYY115 pKa = 10.03PHH117 pKa = 7.25DD118 pKa = 4.72RR119 pKa = 11.84PLSIKK124 pKa = 10.74LSTTYY129 pKa = 11.29DD130 pKa = 3.23DD131 pKa = 4.05GVYY134 pKa = 10.78GNRR137 pKa = 11.84EE138 pKa = 3.64HH139 pKa = 7.64PFRR142 pKa = 11.84GNLTVTINDD151 pKa = 3.27NQTFIIKK158 pKa = 10.43GSRR161 pKa = 11.84NCEE164 pKa = 4.03FIFNPTTEE172 pKa = 4.07GLQNIKK178 pKa = 10.6VAVLDD183 pKa = 4.33SSDD186 pKa = 3.28EE187 pKa = 4.2PYY189 pKa = 10.84YY190 pKa = 11.31GEE192 pKa = 4.47GDD194 pKa = 3.59VSFTYY199 pKa = 10.84YY200 pKa = 10.68RR201 pKa = 11.84FDD203 pKa = 3.4TRR205 pKa = 11.84IVNLDD210 pKa = 3.38DD211 pKa = 3.6TATGHH216 pKa = 6.23VDD218 pKa = 3.0EE219 pKa = 5.46ALNISSYY226 pKa = 10.76LQYY229 pKa = 11.52DD230 pKa = 4.23DD231 pKa = 5.11IYY233 pKa = 9.94TGFVNFEE240 pKa = 4.15DD241 pKa = 4.28EE242 pKa = 5.12ANVTLQINDD251 pKa = 3.53EE252 pKa = 4.47VIPLLVEE259 pKa = 4.69DD260 pKa = 5.82GIISYY265 pKa = 10.7NYY267 pKa = 8.04TPQSVGTYY275 pKa = 10.28NITLTYY281 pKa = 10.72GGDD284 pKa = 3.43SYY286 pKa = 11.46FRR288 pKa = 11.84PSNKK292 pKa = 8.92SWNYY296 pKa = 8.18TVNPYY301 pKa = 8.02EE302 pKa = 4.18TEE304 pKa = 4.05LVIDD308 pKa = 5.14NIDD311 pKa = 3.88DD312 pKa = 3.89FDD314 pKa = 3.82NTVYY318 pKa = 10.98VDD320 pKa = 3.91DD321 pKa = 4.51GVVFNLTLRR330 pKa = 11.84DD331 pKa = 3.61SRR333 pKa = 11.84TKK335 pKa = 10.48KK336 pKa = 10.41AIPNAEE342 pKa = 3.29IRR344 pKa = 11.84VRR346 pKa = 11.84HH347 pKa = 6.42DD348 pKa = 3.08SDD350 pKa = 3.99EE351 pKa = 4.2YY352 pKa = 11.25TITTDD357 pKa = 3.75DD358 pKa = 4.21GGHH361 pKa = 5.22VSITNFSTTAGLHH374 pKa = 5.91TIDD377 pKa = 4.56FKK379 pKa = 11.84YY380 pKa = 10.65DD381 pKa = 3.03GSAIATPSNNTLSFEE396 pKa = 4.12VSKK399 pKa = 11.1RR400 pKa = 11.84DD401 pKa = 4.43LEE403 pKa = 4.3LTLDD407 pKa = 5.0DD408 pKa = 4.33IDD410 pKa = 5.17SVTLYY415 pKa = 10.71SQALIQGDD423 pKa = 4.08VLNLSGNDD431 pKa = 3.44DD432 pKa = 3.95VEE434 pKa = 4.5VVIVIDD440 pKa = 3.97DD441 pKa = 3.77QNYY444 pKa = 6.96TASFTNQTHH453 pKa = 5.36YY454 pKa = 10.89SYY456 pKa = 11.29YY457 pKa = 8.83YY458 pKa = 8.37TALSVGTHH466 pKa = 5.13NVSVYY471 pKa = 8.71TVEE474 pKa = 3.9NYY476 pKa = 10.36KK477 pKa = 10.42YY478 pKa = 10.23LQSNVVNSSFTVEE491 pKa = 5.06KK492 pKa = 10.12IKK494 pKa = 9.39TWIHH498 pKa = 6.42LDD500 pKa = 3.36DD501 pKa = 4.03STFSGIRR508 pKa = 11.84DD509 pKa = 3.72FSVDD513 pKa = 4.11DD514 pKa = 4.55SIKK517 pKa = 10.38MIAQLEE523 pKa = 4.42TGDD526 pKa = 4.99DD527 pKa = 4.01EE528 pKa = 4.89EE529 pKa = 6.32DD530 pKa = 3.35SGNILLFNITGDD542 pKa = 3.99GISDD546 pKa = 4.25DD547 pKa = 3.84EE548 pKa = 4.36LSYY551 pKa = 11.52NVVRR555 pKa = 11.84NANWYY560 pKa = 9.28HH561 pKa = 5.98IQFTMIPAGEE571 pKa = 4.13YY572 pKa = 10.73NITAYY577 pKa = 9.52TRR579 pKa = 11.84DD580 pKa = 3.71TDD582 pKa = 3.33NYY584 pKa = 8.24MYY586 pKa = 11.25SMNSTIITVHH596 pKa = 6.72KK597 pKa = 9.39GDD599 pKa = 3.66VMIDD603 pKa = 3.2ASLSEE608 pKa = 4.79DD609 pKa = 3.72SVALYY614 pKa = 11.23SNITLDD620 pKa = 3.65VSLEE624 pKa = 3.94DD625 pKa = 3.91SEE627 pKa = 5.39GEE629 pKa = 4.06KK630 pKa = 10.61LVNHH634 pKa = 6.79PFVIEE639 pKa = 3.96VDD641 pKa = 3.49GQTFDD646 pKa = 3.54TKK648 pKa = 10.55TGEE651 pKa = 4.46DD652 pKa = 3.44GLATFTYY659 pKa = 8.46QTQHH663 pKa = 5.85TGNTMVTVRR672 pKa = 11.84VDD674 pKa = 3.11EE675 pKa = 4.53SYY677 pKa = 11.16SYY679 pKa = 11.58YY680 pKa = 10.75EE681 pKa = 3.87STRR684 pKa = 11.84NLTFNTYY691 pKa = 10.57LLNTVIEE698 pKa = 4.51VNATDD703 pKa = 4.46SIVGKK708 pKa = 10.28NSTITVTLLDD718 pKa = 3.88EE719 pKa = 5.59LSNKK723 pKa = 9.93LNNQTLMIEE732 pKa = 4.53VNGQTSTVTTDD743 pKa = 3.19DD744 pKa = 3.51TGCAVILYY752 pKa = 6.63PTTSVGLNNVTVTFNPTTKK771 pKa = 10.82YY772 pKa = 11.43NMTSTTTQFNVYY784 pKa = 7.52RR785 pKa = 11.84HH786 pKa = 4.97KK787 pKa = 9.87TIINLTTTDD796 pKa = 2.76IKK798 pKa = 10.97INQTEE803 pKa = 4.27VLSGSLSDD811 pKa = 4.59EE812 pKa = 4.68DD813 pKa = 5.33GEE815 pKa = 4.32PLANALVDD823 pKa = 4.06LSVNGDD829 pKa = 3.74VIIIPTDD836 pKa = 3.11EE837 pKa = 4.45LGRR840 pKa = 11.84YY841 pKa = 8.0SYY843 pKa = 11.06EE844 pKa = 4.07YY845 pKa = 10.39IPVNSGMHH853 pKa = 5.18TVTVDD858 pKa = 3.9FYY860 pKa = 11.65EE861 pKa = 4.84DD862 pKa = 4.13DD863 pKa = 4.0YY864 pKa = 12.1YY865 pKa = 11.83NDD867 pKa = 3.77SKK869 pKa = 11.83VEE871 pKa = 3.97DD872 pKa = 3.8SFNVTKK878 pKa = 10.85YY879 pKa = 9.22DD880 pKa = 3.34TTLTVDD886 pKa = 3.69YY887 pKa = 10.51TPITYY892 pKa = 9.85VQDD895 pKa = 3.48SARR898 pKa = 11.84TSIQGILTDD907 pKa = 3.71EE908 pKa = 4.78LEE910 pKa = 4.63NPVTAAKK917 pKa = 9.89IKK919 pKa = 10.58LVIDD923 pKa = 3.96GGEE926 pKa = 3.91HH927 pKa = 6.66DD928 pKa = 4.85VYY930 pKa = 10.82TIEE933 pKa = 5.08DD934 pKa = 3.14GSYY937 pKa = 8.8QYY939 pKa = 11.27SYY941 pKa = 9.46KK942 pKa = 8.54TTVNGTKK949 pKa = 10.17SVTAVYY955 pKa = 10.48GGDD958 pKa = 3.16NYY960 pKa = 11.35YY961 pKa = 11.0NPSKK965 pKa = 10.66DD966 pKa = 3.47VQTSFSVTFSTPDD979 pKa = 3.23DD980 pKa = 4.01TPVDD984 pKa = 3.4NHH986 pKa = 6.64TDD988 pKa = 3.95DD989 pKa = 5.6NPTTNNTPATNQTNTTTDD1007 pKa = 3.33NNSSEE1012 pKa = 4.08GSDD1015 pKa = 3.39VNINVTNGTSEE1026 pKa = 4.12NNNVSSNISDD1036 pKa = 3.3VTANHH1041 pKa = 6.78TNTTTNNTNTTANVTNTTSNMTNTTQSDD1069 pKa = 4.17TNTTGNATNIDD1080 pKa = 3.39INATNNTNVNNTNVTGNSSDD1100 pKa = 3.35VDD1102 pKa = 4.09VNSTTDD1108 pKa = 3.38TNITDD1113 pKa = 3.53SDD1115 pKa = 4.13VTGNASDD1122 pKa = 4.36VDD1124 pKa = 4.24VNDD1127 pKa = 3.92TNGTIVDD1134 pKa = 3.86NSNVTGNSSGDD1145 pKa = 3.78VVNGTSDD1152 pKa = 3.68TNITDD1157 pKa = 3.89SNITGNGSGVVTNNTDD1173 pKa = 3.14NTTVNATNVTGNTSEE1188 pKa = 4.29VVTNGTNGTSVDD1200 pKa = 3.55NSNVTGNSSGDD1211 pKa = 3.69VVNGSSDD1218 pKa = 3.69TNITDD1223 pKa = 3.9SNITGNSSGVVTNNTNSTAVNDD1245 pKa = 4.04TNMTGNSTDD1254 pKa = 3.64VIVNGTNGTNITDD1267 pKa = 3.75SNITGNSTDD1276 pKa = 3.17VVTNSTNATGVDD1288 pKa = 3.5NSNVTGNSSGVVSNDD1303 pKa = 2.97TGGTNITNSTITGNTTNTTEE1323 pKa = 3.84NSANTTIEE1331 pKa = 3.84NSNITTNNTNSTINQNNTNITNVTINDD1358 pKa = 3.64TSSNNTVNSTNSTHH1372 pKa = 5.35TNEE1375 pKa = 5.0NITNNTVNNTTIDD1388 pKa = 3.35SSTEE1392 pKa = 3.68NGTQPISGNVTPNATDD1408 pKa = 3.36VPSNVTNNTTVVDD1421 pKa = 4.21SNDD1424 pKa = 3.22TKK1426 pKa = 11.01TSDD1429 pKa = 3.82EE1430 pKa = 4.66NITGNTSPPDD1440 pKa = 3.83AQNSTSDD1447 pKa = 3.11QTDD1450 pKa = 3.27IEE1452 pKa = 4.42NSTVAEE1458 pKa = 4.13NEE1460 pKa = 4.16TLNNTNVTSNEE1471 pKa = 4.07TIEE1474 pKa = 4.63DD1475 pKa = 4.02SNVTEE1480 pKa = 5.37DD1481 pKa = 3.17VTANDD1486 pKa = 3.84TVNNTNVTEE1495 pKa = 4.1NDD1497 pKa = 3.58TVVDD1501 pKa = 4.08SNVTEE1506 pKa = 4.07NATVNDD1512 pKa = 4.0TVDD1515 pKa = 3.66YY1516 pKa = 11.13TNVTSNDD1523 pKa = 3.63TIEE1526 pKa = 4.77DD1527 pKa = 3.95SNVTEE1532 pKa = 4.16NVTVNDD1538 pKa = 4.05TVNNTDD1544 pKa = 3.07VTVNGTVEE1552 pKa = 4.03NSNVTEE1558 pKa = 4.67DD1559 pKa = 3.29VTANDD1564 pKa = 4.25SVNSTNVTEE1573 pKa = 4.3NVTVVDD1579 pKa = 4.12SNVTEE1584 pKa = 4.09NVTVNDD1590 pKa = 3.92TVNNTNVTTNVTIEE1604 pKa = 4.31DD1605 pKa = 4.15SNVTTNVTVNDD1616 pKa = 4.11TVNSSEE1622 pKa = 4.17NVTINQTTNVTVGDD1636 pKa = 3.77NSSVVNQSSTDD1647 pKa = 3.47VNNNADD1653 pKa = 3.78MNNDD1657 pKa = 3.5RR1658 pKa = 11.84QHH1660 pKa = 6.69EE1661 pKa = 4.17DD1662 pKa = 3.25SSDD1665 pKa = 3.63SEE1667 pKa = 4.86AGDD1670 pKa = 3.95GSDD1673 pKa = 5.1VSDD1676 pKa = 5.5DD1677 pKa = 4.4NINTNPVDD1685 pKa = 3.64VSGGDD1690 pKa = 3.5NPSGNPSSDD1699 pKa = 3.29DD1700 pKa = 3.54TQSEE1704 pKa = 4.64DD1705 pKa = 3.54NSNDD1709 pKa = 3.31NPEE1712 pKa = 3.98SSEE1715 pKa = 4.19TQEE1718 pKa = 4.95SEE1720 pKa = 4.71SNPNPSDD1727 pKa = 3.7SQDD1730 pKa = 3.22VSSVSDD1736 pKa = 3.32NSQPVSDD1743 pKa = 4.07VASVRR1748 pKa = 11.84SSVASTASSGVVMASVADD1766 pKa = 3.73VASYY1770 pKa = 11.03AIVDD1774 pKa = 4.18SVVSDD1779 pKa = 3.74AAVEE1783 pKa = 4.3TVDD1786 pKa = 3.31KK1787 pKa = 10.74KK1788 pKa = 11.06AYY1790 pKa = 9.06EE1791 pKa = 4.15LSEE1794 pKa = 4.02MDD1796 pKa = 4.53TFFDD1800 pKa = 4.82DD1801 pKa = 4.53LKK1803 pKa = 11.45NFAKK1807 pKa = 10.62YY1808 pKa = 10.07FIFIFGCIGIGYY1820 pKa = 6.44TYY1822 pKa = 11.22KK1823 pKa = 10.09MSKK1826 pKa = 10.14

Molecular weight:
196.88 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A328S9L7|A0A328S9L7_9EURY Adhesin OS=Methanosphaera sp. rholeuAM74 OX=1945579 GN=BZ136_01360 PE=4 SV=1
MM1 pKa = 7.63SKK3 pKa = 10.67NKK5 pKa = 9.58QLPKK9 pKa = 10.39KK10 pKa = 9.96IRR12 pKa = 11.84LAKK15 pKa = 9.02KK16 pKa = 7.29TKK18 pKa = 7.69QNRR21 pKa = 11.84RR22 pKa = 11.84VPIFAMMKK30 pKa = 7.54TSRR33 pKa = 11.84RR34 pKa = 11.84LRR36 pKa = 11.84THH38 pKa = 6.3PKK40 pKa = 8.25ARR42 pKa = 11.84QWSRR46 pKa = 11.84SKK48 pKa = 10.88IKK50 pKa = 10.61VV51 pKa = 3.14

Molecular weight:
6.19 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1765

0

1765

508127

25

3292

287.9

32.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.417 ± 0.069

1.15 ± 0.027

6.611 ± 0.071

6.574 ± 0.089

3.503 ± 0.043

6.016 ± 0.068

1.643 ± 0.029

9.122 ± 0.077

7.023 ± 0.074

8.206 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.038

7.51 ± 0.154

2.891 ± 0.04

2.748 ± 0.046

3.275 ± 0.054

6.534 ± 0.064

7.514 ± 0.131

6.916 ± 0.082

0.58 ± 0.017

4.482 ± 0.053

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski