Grapevine leafroll-associated virus 5

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus; unclassified Ampelovirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q91U90|Q91U90_9CLOS CPd OS=Grapevine leafroll-associated virus 5 OX=71032 GN=CPm PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 4.12VTLCLSKK9 pKa = 11.05VKK11 pKa = 10.41EE12 pKa = 4.25KK13 pKa = 11.33LPVTCDD19 pKa = 3.04EE20 pKa = 4.26IFYY23 pKa = 10.66LRR25 pKa = 11.84NAGVYY30 pKa = 8.03ATVMPPEE37 pKa = 4.39RR38 pKa = 11.84LSFFYY43 pKa = 10.95DD44 pKa = 3.19KK45 pKa = 11.41LSVGNNNDD53 pKa = 3.3RR54 pKa = 11.84NKK56 pKa = 10.34YY57 pKa = 9.57RR58 pKa = 11.84EE59 pKa = 4.14LFQFLDD65 pKa = 3.35NLGNVVATNNGNFIKK80 pKa = 10.54FLDD83 pKa = 3.73VDD85 pKa = 3.95SVMEE89 pKa = 4.02YY90 pKa = 10.39FRR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84YY95 pKa = 9.2PDD97 pKa = 3.37GGNLSSINKK106 pKa = 8.37TPSSDD111 pKa = 3.95YY112 pKa = 11.27CCLTVPALTTFLNSVCGCIEE132 pKa = 3.94GLTTVLAVGFTRR144 pKa = 11.84KK145 pKa = 9.74SDD147 pKa = 3.61TDD149 pKa = 3.74EE150 pKa = 3.93LWSFKK155 pKa = 9.71NTFVVFEE162 pKa = 4.12NSGEE166 pKa = 3.84ISTFYY171 pKa = 10.37GDD173 pKa = 5.9SMDD176 pKa = 3.76TSNSALLEE184 pKa = 4.12VLDD187 pKa = 4.66SFGSTSGKK195 pKa = 8.61TDD197 pKa = 3.58FEE199 pKa = 4.65KK200 pKa = 11.03VVSLLSII207 pKa = 4.28

Molecular weight: 23.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4U4B0|G4U4B0_9CLOS Coat protein OS=Grapevine leafroll-associated virus 5 OX=71032 GN=CP PE=4 SV=1
MM1 pKa = 7.53ALSATSTYY9 pKa = 10.67VRR11 pKa = 11.84VSKK14 pKa = 10.8SDD16 pKa = 3.31EE17 pKa = 4.07GFVNLLRR24 pKa = 11.84TFKK27 pKa = 11.28GEE29 pKa = 3.91NDD31 pKa = 2.74VDD33 pKa = 3.8AFADD37 pKa = 3.8EE38 pKa = 4.25VLNYY42 pKa = 10.64LDD44 pKa = 5.07LRR46 pKa = 11.84FSAISGRR53 pKa = 11.84FSLGNYY59 pKa = 9.2SCNTWYY65 pKa = 10.89NGVSGAVGAWPDD77 pKa = 3.37SEE79 pKa = 3.89GWGRR83 pKa = 11.84HH84 pKa = 4.73LLANYY89 pKa = 9.24IVSNNALAHH98 pKa = 6.63LYY100 pKa = 10.36YY101 pKa = 10.31PLASEE106 pKa = 4.46IKK108 pKa = 9.82SAVATPMSVFTDD120 pKa = 3.1KK121 pKa = 10.95LSRR124 pKa = 11.84VGKK127 pKa = 9.29PVTEE131 pKa = 3.98TSKK134 pKa = 10.98FPFKK138 pKa = 10.61VSRR141 pKa = 11.84VEE143 pKa = 4.06AEE145 pKa = 3.85KK146 pKa = 10.77KK147 pKa = 9.84AAEE150 pKa = 4.06FGKK153 pKa = 8.31PTKK156 pKa = 9.34HH157 pKa = 5.12TQDD160 pKa = 4.61LIFCIGNYY168 pKa = 9.92LGRR171 pKa = 11.84LPTKK175 pKa = 10.4DD176 pKa = 3.44EE177 pKa = 4.22VMGMTSLPVAAVRR190 pKa = 11.84KK191 pKa = 9.91GSLLLDD197 pKa = 3.63VGSDD201 pKa = 3.33VTSNEE206 pKa = 3.86RR207 pKa = 11.84KK208 pKa = 9.64LATLNLRR215 pKa = 11.84GKK217 pKa = 9.53NKK219 pKa = 10.18QGDD222 pKa = 3.78PVEE225 pKa = 4.47GVFFKK230 pKa = 10.51TRR232 pKa = 11.84QTEE235 pKa = 4.07IEE237 pKa = 4.38TVASFIFDD245 pKa = 3.37NKK247 pKa = 9.6IVKK250 pKa = 7.72EE251 pKa = 4.18TLSLPGVQWTILSCLKK267 pKa = 10.17KK268 pKa = 10.61YY269 pKa = 10.41PVFADD274 pKa = 3.51SFEE277 pKa = 4.36DD278 pKa = 4.47RR279 pKa = 11.84IDD281 pKa = 3.54SLVYY285 pKa = 10.13VQAQVRR291 pKa = 11.84KK292 pKa = 9.29LLDD295 pKa = 3.23GLIPFKK301 pKa = 11.04EE302 pKa = 5.06DD303 pKa = 2.88IDD305 pKa = 4.64LDD307 pKa = 3.98AALSSGTLLKK317 pKa = 11.26GEE319 pKa = 4.42VFRR322 pKa = 11.84GDD324 pKa = 3.5AKK326 pKa = 11.15VLTPRR331 pKa = 11.84FQRR334 pKa = 11.84NTTGGLLMRR343 pKa = 11.84IGSRR347 pKa = 11.84FSVSEE352 pKa = 3.86YY353 pKa = 11.12SEE355 pKa = 4.12LTRR358 pKa = 11.84KK359 pKa = 9.7LVGALLNRR367 pKa = 11.84NLSMDD372 pKa = 3.39VTYY375 pKa = 9.2TQAVLILLQRR385 pKa = 11.84YY386 pKa = 6.9IHH388 pKa = 6.1YY389 pKa = 7.76RR390 pKa = 11.84TNALRR395 pKa = 11.84YY396 pKa = 8.91VNLPAKK402 pKa = 10.01LDD404 pKa = 3.61YY405 pKa = 10.2MIKK408 pKa = 10.54NEE410 pKa = 3.92VKK412 pKa = 9.98QVDD415 pKa = 4.09FSGVDD420 pKa = 3.52EE421 pKa = 4.78VFSKK425 pKa = 11.13YY426 pKa = 10.07QGTIPEE432 pKa = 4.81IEE434 pKa = 4.44RR435 pKa = 11.84AWCAPLADD443 pKa = 3.75VAYY446 pKa = 9.66MFLKK450 pKa = 10.12DD451 pKa = 3.55TGGSFAKK458 pKa = 9.92WKK460 pKa = 10.32DD461 pKa = 3.72VQDD464 pKa = 4.21VPAHH468 pKa = 5.63MNFDD472 pKa = 3.65FVGFVDD478 pKa = 4.82PKK480 pKa = 10.81LLSDD484 pKa = 5.6HH485 pKa = 6.25EE486 pKa = 4.89LKK488 pKa = 10.98CQTRR492 pKa = 11.84LLDD495 pKa = 3.53RR496 pKa = 11.84FRR498 pKa = 11.84SKK500 pKa = 10.06DD501 pKa = 3.31TPVRR505 pKa = 11.84GFLLGARR512 pKa = 11.84KK513 pKa = 9.77GNPVDD518 pKa = 4.01YY519 pKa = 9.62LASSAGIGGIEE530 pKa = 3.77KK531 pKa = 10.91AMVKK535 pKa = 10.41KK536 pKa = 10.96LIGG539 pKa = 3.55

Molecular weight: 59.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4352

46

2241

621.7

68.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.457 ± 0.308

1.93 ± 0.248

5.561 ± 0.496

5.699 ± 0.319

5.17 ± 0.817

6.043 ± 0.462

1.517 ± 0.381

5.055 ± 0.366

7.146 ± 0.594

9.099 ± 0.427

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.178

5.446 ± 0.284

4.251 ± 0.278

2.229 ± 0.182

4.986 ± 0.16

9.513 ± 0.577

5.767 ± 0.288

8.456 ± 0.59

0.735 ± 0.144

2.849 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski