Calothrix sp. NIES-4101

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; unclassified Calothrix

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4RAY7|A0A1Z4RAY7_9CYAN S-adenosylmethionine synthetase OS=Calothrix sp. NIES-4101 OX=2005461 GN=NIES4101_55780 PE=3 SV=1
MM1 pKa = 7.51TEE3 pKa = 3.49QTIYY7 pKa = 11.35DD8 pKa = 4.31EE9 pKa = 4.72ILGEE13 pKa = 4.48LAWDD17 pKa = 5.0DD18 pKa = 6.05DD19 pKa = 4.51DD20 pKa = 5.35EE21 pKa = 4.8CWSSQVQFTLEE32 pKa = 4.29HH33 pKa = 6.14IVNVTIDD40 pKa = 3.81PDD42 pKa = 3.87DD43 pKa = 5.02AEE45 pKa = 4.35TGAVIMKK52 pKa = 9.85SRR54 pKa = 11.84QSFAKK59 pKa = 10.35LQEE62 pKa = 3.93NEE64 pKa = 3.85LNIRR68 pKa = 11.84RR69 pKa = 11.84LISDD73 pKa = 3.59QMLKK77 pKa = 10.07IYY79 pKa = 10.51NDD81 pKa = 2.8NWNNGEE87 pKa = 4.74IIDD90 pKa = 4.08TQDD93 pKa = 3.93FIDD96 pKa = 4.47SIKK99 pKa = 11.12LEE101 pKa = 4.97DD102 pKa = 3.28IVFYY106 pKa = 11.26ANGNIQLFYY115 pKa = 11.02NDD117 pKa = 4.13GYY119 pKa = 11.33LFAGHH124 pKa = 7.07GIIVSIDD131 pKa = 3.17EE132 pKa = 4.07YY133 pKa = 11.54GNYY136 pKa = 9.31QEE138 pKa = 5.96AVLFGG143 pKa = 3.89

Molecular weight: 16.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4RHE4|A0A1Z4RHE4_9CYAN Uncharacterized protein OS=Calothrix sp. NIES-4101 OX=2005461 GN=NIES4101_78500 PE=4 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTNRR10 pKa = 11.84KK11 pKa = 9.1RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIKK32 pKa = 10.5ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.04GRR39 pKa = 11.84YY40 pKa = 8.47RR41 pKa = 11.84LSVV44 pKa = 3.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6218

0

6218

1974457

29

3238

317.5

35.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.919 ± 0.033

1.021 ± 0.012

4.754 ± 0.023

6.216 ± 0.032

4.042 ± 0.022

6.492 ± 0.03

1.818 ± 0.015

7.289 ± 0.032

5.243 ± 0.028

10.569 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.894 ± 0.016

4.944 ± 0.031

4.493 ± 0.024

5.277 ± 0.032

4.923 ± 0.025

6.575 ± 0.028

5.678 ± 0.029

6.421 ± 0.023

1.379 ± 0.015

3.055 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski