Amino acid dipepetide frequency for Staphylococcus virus phiETA2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.383AlaAla: 1.383 ± 0.415
0.291AlaCys: 0.291 ± 0.147
3.129AlaAsp: 3.129 ± 0.481
4.148AlaGlu: 4.148 ± 0.612
2.838AlaPhe: 2.838 ± 0.512
2.838AlaGly: 2.838 ± 0.437
1.019AlaHis: 1.019 ± 0.257
5.166AlaIle: 5.166 ± 0.913
5.094AlaLys: 5.094 ± 0.672
3.784AlaLeu: 3.784 ± 0.634
2.11AlaMet: 2.11 ± 0.464
3.711AlaAsn: 3.711 ± 0.46
1.383AlaPro: 1.383 ± 0.255
2.183AlaGln: 2.183 ± 0.495
2.474AlaArg: 2.474 ± 0.48
3.129AlaSer: 3.129 ± 0.595
3.565AlaThr: 3.565 ± 0.585
3.857AlaVal: 3.857 ± 0.598
0.873AlaTrp: 0.873 ± 0.262
2.328AlaTyr: 2.328 ± 0.359
0.0AlaXaa: 0.0 ± 0.0
Cys
0.291CysAla: 0.291 ± 0.141
0.0CysCys: 0.0 ± 0.0
0.146CysAsp: 0.146 ± 0.138
0.437CysGlu: 0.437 ± 0.22
0.364CysPhe: 0.364 ± 0.165
0.146CysGly: 0.146 ± 0.116
0.073CysHis: 0.073 ± 0.054
0.218CysIle: 0.218 ± 0.141
0.582CysLys: 0.582 ± 0.231
0.509CysLeu: 0.509 ± 0.224
0.146CysMet: 0.146 ± 0.154
0.364CysAsn: 0.364 ± 0.164
0.146CysPro: 0.146 ± 0.1
0.218CysGln: 0.218 ± 0.121
0.437CysArg: 0.437 ± 0.216
0.364CysSer: 0.364 ± 0.172
0.218CysThr: 0.218 ± 0.121
0.073CysVal: 0.073 ± 0.085
0.073CysTrp: 0.073 ± 0.071
0.146CysTyr: 0.146 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
3.42AspAla: 3.42 ± 0.591
0.364AspCys: 0.364 ± 0.15
4.293AspAsp: 4.293 ± 0.747
5.967AspGlu: 5.967 ± 1.001
4.075AspPhe: 4.075 ± 0.653
3.711AspGly: 3.711 ± 0.612
0.364AspHis: 0.364 ± 0.197
4.802AspIle: 4.802 ± 0.636
5.967AspLys: 5.967 ± 0.741
5.312AspLeu: 5.312 ± 0.765
1.819AspMet: 1.819 ± 0.382
3.784AspAsn: 3.784 ± 0.516
1.237AspPro: 1.237 ± 0.287
1.091AspGln: 1.091 ± 0.265
2.256AspArg: 2.256 ± 0.404
3.929AspSer: 3.929 ± 0.518
3.056AspThr: 3.056 ± 0.571
4.511AspVal: 4.511 ± 0.565
0.728AspTrp: 0.728 ± 0.207
2.256AspTyr: 2.256 ± 0.473
0.0AspXaa: 0.0 ± 0.0
Glu
5.312GluAla: 5.312 ± 0.771
0.509GluCys: 0.509 ± 0.191
3.929GluAsp: 3.929 ± 0.623
6.258GluGlu: 6.258 ± 0.804
3.056GluPhe: 3.056 ± 0.498
3.347GluGly: 3.347 ± 0.515
1.528GluHis: 1.528 ± 0.305
5.676GluIle: 5.676 ± 0.859
6.258GluLys: 6.258 ± 0.908
7.786GluLeu: 7.786 ± 0.969
2.183GluMet: 2.183 ± 0.411
4.439GluAsn: 4.439 ± 0.571
1.892GluPro: 1.892 ± 0.439
4.22GluGln: 4.22 ± 0.66
3.929GluArg: 3.929 ± 0.705
3.857GluSer: 3.857 ± 0.603
3.274GluThr: 3.274 ± 0.489
4.875GluVal: 4.875 ± 0.678
0.582GluTrp: 0.582 ± 0.217
5.094GluTyr: 5.094 ± 0.835
0.0GluXaa: 0.0 ± 0.0
Phe
2.11PheAla: 2.11 ± 0.384
0.364PheCys: 0.364 ± 0.144
3.056PheAsp: 3.056 ± 0.541
4.002PheGlu: 4.002 ± 0.528
1.091PhePhe: 1.091 ± 0.296
3.129PheGly: 3.129 ± 0.449
0.873PheHis: 0.873 ± 0.287
3.202PheIle: 3.202 ± 0.476
3.565PheLys: 3.565 ± 0.52
2.328PheLeu: 2.328 ± 0.438
1.31PheMet: 1.31 ± 0.293
3.274PheAsn: 3.274 ± 0.42
1.164PhePro: 1.164 ± 0.32
1.746PheGln: 1.746 ± 0.349
1.383PheArg: 1.383 ± 0.292
3.202PheSer: 3.202 ± 0.509
3.347PheThr: 3.347 ± 0.466
1.965PheVal: 1.965 ± 0.445
0.146PheTrp: 0.146 ± 0.102
1.674PheTyr: 1.674 ± 0.304
0.0PheXaa: 0.0 ± 0.0
Gly
2.838GlyAla: 2.838 ± 0.536
0.437GlyCys: 0.437 ± 0.183
3.493GlyAsp: 3.493 ± 0.568
3.493GlyGlu: 3.493 ± 0.548
2.328GlyPhe: 2.328 ± 0.355
2.983GlyGly: 2.983 ± 0.547
1.455GlyHis: 1.455 ± 0.369
4.293GlyIle: 4.293 ± 0.53
4.802GlyLys: 4.802 ± 0.626
5.748GlyLeu: 5.748 ± 0.703
1.455GlyMet: 1.455 ± 0.29
3.493GlyAsn: 3.493 ± 0.569
0.437GlyPro: 0.437 ± 0.216
1.674GlyGln: 1.674 ± 0.316
1.965GlyArg: 1.965 ± 0.413
2.911GlySer: 2.911 ± 0.468
3.857GlyThr: 3.857 ± 0.57
4.657GlyVal: 4.657 ± 0.625
0.873GlyTrp: 0.873 ± 0.268
2.692GlyTyr: 2.692 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
1.455HisAla: 1.455 ± 0.396
0.218HisCys: 0.218 ± 0.13
1.237HisAsp: 1.237 ± 0.263
1.31HisGlu: 1.31 ± 0.358
1.091HisPhe: 1.091 ± 0.25
1.528HisGly: 1.528 ± 0.32
0.582HisHis: 0.582 ± 0.229
1.383HisIle: 1.383 ± 0.439
0.946HisLys: 0.946 ± 0.264
1.383HisLeu: 1.383 ± 0.334
0.218HisMet: 0.218 ± 0.155
1.237HisAsn: 1.237 ± 0.329
0.509HisPro: 0.509 ± 0.178
0.509HisGln: 0.509 ± 0.201
0.437HisArg: 0.437 ± 0.181
1.237HisSer: 1.237 ± 0.288
0.8HisThr: 0.8 ± 0.25
1.019HisVal: 1.019 ± 0.329
0.073HisTrp: 0.073 ± 0.085
0.946HisTyr: 0.946 ± 0.384
0.0HisXaa: 0.0 ± 0.0
Ile
4.802IleAla: 4.802 ± 0.687
0.146IleCys: 0.146 ± 0.099
5.239IleAsp: 5.239 ± 0.586
6.403IleGlu: 6.403 ± 0.674
3.056IlePhe: 3.056 ± 0.5
3.784IleGly: 3.784 ± 0.643
1.528IleHis: 1.528 ± 0.27
4.366IleIle: 4.366 ± 0.638
7.567IleLys: 7.567 ± 0.68
4.511IleLeu: 4.511 ± 0.509
2.183IleMet: 2.183 ± 0.388
4.948IleAsn: 4.948 ± 0.607
2.183IlePro: 2.183 ± 0.329
2.037IleGln: 2.037 ± 0.412
3.929IleArg: 3.929 ± 0.662
5.021IleSer: 5.021 ± 0.59
4.802IleThr: 4.802 ± 0.667
4.366IleVal: 4.366 ± 0.597
1.383IleTrp: 1.383 ± 0.569
2.911IleTyr: 2.911 ± 0.472
0.0IleXaa: 0.0 ± 0.0
Lys
6.33LysAla: 6.33 ± 0.666
0.146LysCys: 0.146 ± 0.156
5.967LysAsp: 5.967 ± 0.713
7.276LysGlu: 7.276 ± 0.927
3.565LysPhe: 3.565 ± 0.541
4.73LysGly: 4.73 ± 0.735
1.965LysHis: 1.965 ± 0.407
6.403LysIle: 6.403 ± 0.658
8.586LysLys: 8.586 ± 0.804
6.258LysLeu: 6.258 ± 0.713
2.183LysMet: 2.183 ± 0.464
5.457LysAsn: 5.457 ± 0.638
3.129LysPro: 3.129 ± 0.556
4.948LysGln: 4.948 ± 0.581
3.929LysArg: 3.929 ± 0.661
5.312LysSer: 5.312 ± 0.583
5.385LysThr: 5.385 ± 0.602
5.967LysVal: 5.967 ± 0.579
0.582LysTrp: 0.582 ± 0.186
5.094LysTyr: 5.094 ± 0.692
0.0LysXaa: 0.0 ± 0.0
Leu
3.638LeuAla: 3.638 ± 0.647
0.364LeuCys: 0.364 ± 0.215
3.857LeuAsp: 3.857 ± 0.685
6.549LeuGlu: 6.549 ± 0.71
3.638LeuPhe: 3.638 ± 0.546
3.638LeuGly: 3.638 ± 0.511
0.8LeuHis: 0.8 ± 0.3
4.439LeuIle: 4.439 ± 0.465
7.058LeuLys: 7.058 ± 0.553
5.967LeuLeu: 5.967 ± 0.677
1.383LeuMet: 1.383 ± 0.338
5.385LeuAsn: 5.385 ± 0.602
2.547LeuPro: 2.547 ± 0.389
3.347LeuGln: 3.347 ± 0.478
4.002LeuArg: 4.002 ± 0.635
4.73LeuSer: 4.73 ± 0.572
4.948LeuThr: 4.948 ± 0.687
4.439LeuVal: 4.439 ± 0.614
0.8LeuTrp: 0.8 ± 0.245
3.565LeuTyr: 3.565 ± 0.487
0.0LeuXaa: 0.0 ± 0.0
Met
1.164MetAla: 1.164 ± 0.266
0.218MetCys: 0.218 ± 0.122
1.019MetAsp: 1.019 ± 0.256
1.746MetGlu: 1.746 ± 0.478
0.873MetPhe: 0.873 ± 0.286
1.019MetGly: 1.019 ± 0.295
0.655MetHis: 0.655 ± 0.238
1.528MetIle: 1.528 ± 0.34
2.183MetLys: 2.183 ± 0.457
2.401MetLeu: 2.401 ± 0.36
0.655MetMet: 0.655 ± 0.226
2.328MetAsn: 2.328 ± 0.384
0.873MetPro: 0.873 ± 0.26
1.383MetGln: 1.383 ± 0.367
1.091MetArg: 1.091 ± 0.351
1.892MetSer: 1.892 ± 0.435
2.765MetThr: 2.765 ± 0.473
0.873MetVal: 0.873 ± 0.24
0.437MetTrp: 0.437 ± 0.18
1.091MetTyr: 1.091 ± 0.286
0.0MetXaa: 0.0 ± 0.0
Asn
4.875AsnAla: 4.875 ± 0.787
0.218AsnCys: 0.218 ± 0.137
4.657AsnAsp: 4.657 ± 0.569
5.094AsnGlu: 5.094 ± 0.628
2.911AsnPhe: 2.911 ± 0.67
5.676AsnGly: 5.676 ± 0.581
0.873AsnHis: 0.873 ± 0.252
4.075AsnIle: 4.075 ± 0.452
6.622AsnLys: 6.622 ± 0.655
4.73AsnLeu: 4.73 ± 0.651
1.746AsnMet: 1.746 ± 0.336
4.875AsnAsn: 4.875 ± 0.678
2.474AsnPro: 2.474 ± 0.5
2.183AsnGln: 2.183 ± 0.305
2.62AsnArg: 2.62 ± 0.352
3.129AsnSer: 3.129 ± 0.487
3.929AsnThr: 3.929 ± 0.574
4.148AsnVal: 4.148 ± 0.529
0.655AsnTrp: 0.655 ± 0.243
3.129AsnTyr: 3.129 ± 0.481
0.0AsnXaa: 0.0 ± 0.0
Pro
1.091ProAla: 1.091 ± 0.287
0.218ProCys: 0.218 ± 0.127
1.528ProAsp: 1.528 ± 0.321
2.183ProGlu: 2.183 ± 0.426
1.019ProPhe: 1.019 ± 0.255
1.965ProGly: 1.965 ± 0.439
0.509ProHis: 0.509 ± 0.195
1.746ProIle: 1.746 ± 0.372
3.056ProLys: 3.056 ± 0.69
1.383ProLeu: 1.383 ± 0.299
1.091ProMet: 1.091 ± 0.238
2.11ProAsn: 2.11 ± 0.399
0.582ProPro: 0.582 ± 0.252
1.164ProGln: 1.164 ± 0.282
1.019ProArg: 1.019 ± 0.314
1.746ProSer: 1.746 ± 0.4
1.892ProThr: 1.892 ± 0.407
1.746ProVal: 1.746 ± 0.401
0.073ProTrp: 0.073 ± 0.069
1.601ProTyr: 1.601 ± 0.398
0.0ProXaa: 0.0 ± 0.0
Gln
2.183GlnAla: 2.183 ± 0.43
0.437GlnCys: 0.437 ± 0.184
2.62GlnAsp: 2.62 ± 0.498
2.401GlnGlu: 2.401 ± 0.496
1.455GlnPhe: 1.455 ± 0.346
1.892GlnGly: 1.892 ± 0.353
0.655GlnHis: 0.655 ± 0.188
2.765GlnIle: 2.765 ± 0.421
3.929GlnLys: 3.929 ± 0.501
2.692GlnLeu: 2.692 ± 0.461
1.383GlnMet: 1.383 ± 0.383
1.965GlnAsn: 1.965 ± 0.326
1.383GlnPro: 1.383 ± 0.357
1.601GlnGln: 1.601 ± 0.43
1.819GlnArg: 1.819 ± 0.33
2.256GlnSer: 2.256 ± 0.365
1.819GlnThr: 1.819 ± 0.363
1.965GlnVal: 1.965 ± 0.407
0.364GlnTrp: 0.364 ± 0.162
1.601GlnTyr: 1.601 ± 0.36
0.0GlnXaa: 0.0 ± 0.0
Arg
1.383ArgAla: 1.383 ± 0.301
0.291ArgCys: 0.291 ± 0.162
2.547ArgAsp: 2.547 ± 0.496
3.493ArgGlu: 3.493 ± 0.494
1.892ArgPhe: 1.892 ± 0.464
2.328ArgGly: 2.328 ± 0.41
1.31ArgHis: 1.31 ± 0.349
3.784ArgIle: 3.784 ± 0.566
4.148ArgLys: 4.148 ± 0.602
3.565ArgLeu: 3.565 ± 0.501
0.655ArgMet: 0.655 ± 0.191
3.347ArgAsn: 3.347 ± 0.605
1.019ArgPro: 1.019 ± 0.284
1.237ArgGln: 1.237 ± 0.306
1.819ArgArg: 1.819 ± 0.401
2.183ArgSer: 2.183 ± 0.274
1.892ArgThr: 1.892 ± 0.423
2.401ArgVal: 2.401 ± 0.458
0.509ArgTrp: 0.509 ± 0.204
1.965ArgTyr: 1.965 ± 0.492
0.0ArgXaa: 0.0 ± 0.0
Ser
4.002SerAla: 4.002 ± 0.658
0.146SerCys: 0.146 ± 0.119
4.511SerAsp: 4.511 ± 0.664
3.42SerGlu: 3.42 ± 0.488
2.692SerPhe: 2.692 ± 0.541
3.42SerGly: 3.42 ± 0.591
1.601SerHis: 1.601 ± 0.45
5.676SerIle: 5.676 ± 0.694
5.239SerLys: 5.239 ± 0.688
3.929SerLeu: 3.929 ± 0.46
1.819SerMet: 1.819 ± 0.362
4.439SerAsn: 4.439 ± 0.463
1.091SerPro: 1.091 ± 0.311
1.455SerGln: 1.455 ± 0.304
2.474SerArg: 2.474 ± 0.312
3.638SerSer: 3.638 ± 0.531
3.565SerThr: 3.565 ± 0.396
3.056SerVal: 3.056 ± 0.517
0.291SerTrp: 0.291 ± 0.154
2.037SerTyr: 2.037 ± 0.39
0.0SerXaa: 0.0 ± 0.0
Thr
2.983ThrAla: 2.983 ± 0.493
0.0ThrCys: 0.0 ± 0.0
3.857ThrAsp: 3.857 ± 0.693
4.293ThrGlu: 4.293 ± 0.587
2.474ThrPhe: 2.474 ± 0.494
3.784ThrGly: 3.784 ± 0.544
0.8ThrHis: 0.8 ± 0.225
5.967ThrIle: 5.967 ± 1.126
5.748ThrLys: 5.748 ± 0.576
4.366ThrLeu: 4.366 ± 0.543
1.019ThrMet: 1.019 ± 0.35
4.075ThrAsn: 4.075 ± 0.731
1.965ThrPro: 1.965 ± 0.364
2.474ThrGln: 2.474 ± 0.49
2.037ThrArg: 2.037 ± 0.324
2.983ThrSer: 2.983 ± 0.482
3.638ThrThr: 3.638 ± 0.724
4.73ThrVal: 4.73 ± 0.821
0.873ThrTrp: 0.873 ± 0.272
2.328ThrTyr: 2.328 ± 0.431
0.0ThrXaa: 0.0 ± 0.0
Val
3.42ValAla: 3.42 ± 0.749
0.364ValCys: 0.364 ± 0.138
4.73ValAsp: 4.73 ± 0.642
4.948ValGlu: 4.948 ± 0.762
2.256ValPhe: 2.256 ± 0.457
2.547ValGly: 2.547 ± 0.515
0.509ValHis: 0.509 ± 0.185
5.894ValIle: 5.894 ± 0.608
5.967ValLys: 5.967 ± 0.534
4.22ValLeu: 4.22 ± 0.612
1.601ValMet: 1.601 ± 0.393
4.73ValAsn: 4.73 ± 0.593
2.037ValPro: 2.037 ± 0.459
1.383ValGln: 1.383 ± 0.319
1.892ValArg: 1.892 ± 0.381
4.002ValSer: 4.002 ± 0.539
3.784ValThr: 3.784 ± 0.567
3.857ValVal: 3.857 ± 0.56
0.873ValTrp: 0.873 ± 0.308
2.547ValTyr: 2.547 ± 0.529
0.0ValXaa: 0.0 ± 0.0
Trp
0.728TrpAla: 0.728 ± 0.307
0.073TrpCys: 0.073 ± 0.071
0.509TrpAsp: 0.509 ± 0.216
0.582TrpGlu: 0.582 ± 0.215
0.218TrpPhe: 0.218 ± 0.117
0.655TrpGly: 0.655 ± 0.272
0.073TrpHis: 0.073 ± 0.069
0.873TrpIle: 0.873 ± 0.251
0.873TrpLys: 0.873 ± 0.275
0.8TrpLeu: 0.8 ± 0.288
0.218TrpMet: 0.218 ± 0.119
1.965TrpAsn: 1.965 ± 0.889
0.146TrpPro: 0.146 ± 0.1
0.509TrpGln: 0.509 ± 0.211
0.218TrpArg: 0.218 ± 0.127
0.873TrpSer: 0.873 ± 0.279
1.091TrpThr: 1.091 ± 0.291
0.509TrpVal: 0.509 ± 0.193
0.146TrpTrp: 0.146 ± 0.1
0.509TrpTyr: 0.509 ± 0.215
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.892TyrAla: 1.892 ± 0.347
0.146TyrCys: 0.146 ± 0.113
2.62TyrAsp: 2.62 ± 0.502
4.075TyrGlu: 4.075 ± 0.784
2.037TyrPhe: 2.037 ± 0.468
2.765TyrGly: 2.765 ± 0.536
0.946TyrHis: 0.946 ± 0.253
2.911TyrIle: 2.911 ± 0.442
5.021TyrLys: 5.021 ± 0.627
3.42TyrLeu: 3.42 ± 0.57
0.946TyrMet: 0.946 ± 0.293
2.692TyrAsn: 2.692 ± 0.368
1.528TyrPro: 1.528 ± 0.398
1.819TyrGln: 1.819 ± 0.358
2.037TyrArg: 2.037 ± 0.424
2.11TyrSer: 2.11 ± 0.4
2.765TyrThr: 2.765 ± 0.55
2.547TyrVal: 2.547 ± 0.45
1.237TyrTrp: 1.237 ± 0.395
1.383TyrTyr: 1.383 ± 0.264
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (13744 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski