Microbacterium testaceum (strain StLB037)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; Microbacterium testaceum

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E8N7F3|E8N7F3_MICTS Predicted ferric reductase OS=Microbacterium testaceum (strain StLB037) OX=979556 GN=MTES_0022 PE=4 SV=1
MM1 pKa = 7.32SRR3 pKa = 11.84IPDD6 pKa = 3.56TQMTPEE12 pKa = 4.45GPAYY16 pKa = 9.92EE17 pKa = 4.59GRR19 pKa = 11.84LLDD22 pKa = 4.08RR23 pKa = 11.84ADD25 pKa = 4.12EE26 pKa = 4.22PVVDD30 pKa = 4.31QGAPFDD36 pKa = 3.53IRR38 pKa = 11.84TLVSRR43 pKa = 11.84RR44 pKa = 11.84GILGLVALGAGAVTLVACTPAATGTTTATTATPTATASATPTASATTSAAALPAGEE100 pKa = 5.09IPDD103 pKa = 4.05EE104 pKa = 4.33TAGPYY109 pKa = 10.2PGDD112 pKa = 3.75GSNGADD118 pKa = 3.21VLEE121 pKa = 4.02QSGIVRR127 pKa = 11.84SDD129 pKa = 3.15LRR131 pKa = 11.84SSLDD135 pKa = 3.27GGATAAGTPMTLNLTIFDD153 pKa = 4.28TANGDD158 pKa = 3.74TPMTGAAVYY167 pKa = 9.65VWHH170 pKa = 7.65CDD172 pKa = 3.29TEE174 pKa = 5.0GNYY177 pKa = 11.2SMYY180 pKa = 10.83SSGIEE185 pKa = 3.74NEE187 pKa = 4.14TYY189 pKa = 10.54LRR191 pKa = 11.84GVQVVGDD198 pKa = 4.12DD199 pKa = 3.87GVVSFTSIFPGCYY212 pKa = 9.22DD213 pKa = 3.33GRR215 pKa = 11.84WPHH218 pKa = 6.03IHH220 pKa = 6.34FEE222 pKa = 4.43VYY224 pKa = 10.23PSVDD228 pKa = 3.97AITDD232 pKa = 3.54ASNAIATSQVALPKK246 pKa = 8.18EE247 pKa = 4.5TCDD250 pKa = 3.44VVYY253 pKa = 10.73ADD255 pKa = 3.58TSLYY259 pKa = 9.92PSSASNLTRR268 pKa = 11.84VSLDD272 pKa = 3.04SDD274 pKa = 3.81NVFGDD279 pKa = 4.32DD280 pKa = 4.91GGALQLATTSGDD292 pKa = 3.31NASGYY297 pKa = 7.7TVSLGVRR304 pKa = 11.84VDD306 pKa = 3.45TATTPTAGAAPGGRR320 pKa = 3.66

Molecular weight: 32.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E8NER9|E8NER9_MICTS D-inositol 3-phosphate glycosyltransferase OS=Microbacterium testaceum (strain StLB037) OX=979556 GN=MTES_2190 PE=4 SV=1
MM1 pKa = 6.81GTVPRR6 pKa = 11.84AATGIARR13 pKa = 11.84SVATMMRR20 pKa = 11.84VRR22 pKa = 11.84RR23 pKa = 11.84AMATVRR29 pKa = 11.84SVATTTGVPLEE40 pKa = 4.07MAIVLSVVMVRR51 pKa = 11.84ARR53 pKa = 11.84GVPRR57 pKa = 11.84VMATVLSVATTMRR70 pKa = 11.84VRR72 pKa = 11.84RR73 pKa = 11.84AMATVLSVGTTMRR86 pKa = 11.84GPRR89 pKa = 11.84GMAIVLSVATTMRR102 pKa = 11.84VPRR105 pKa = 11.84VMATVLSAGTTMRR118 pKa = 11.84VRR120 pKa = 11.84RR121 pKa = 11.84GMAIVLSVGTTMRR134 pKa = 11.84GPRR137 pKa = 11.84GMAIVLSVGTTMRR150 pKa = 11.84GPRR153 pKa = 11.84GMAIVLSVATTTGVRR168 pKa = 11.84RR169 pKa = 11.84GTVIVLSVVTASVRR183 pKa = 11.84SDD185 pKa = 3.03ATVMDD190 pKa = 3.63VRR192 pKa = 11.84RR193 pKa = 11.84ARR195 pKa = 11.84VTVGRR200 pKa = 11.84NATAIVLSTVTLVAPVDD217 pKa = 4.08RR218 pKa = 11.84PVMATVHH225 pKa = 5.84SAVTATPPGARR236 pKa = 11.84GVTVSRR242 pKa = 11.84AASAGVTVAPPATVGPVPAHH262 pKa = 6.17LAVSTVRR269 pKa = 11.84TPARR273 pKa = 11.84RR274 pKa = 11.84DD275 pKa = 3.4RR276 pKa = 11.84LRR278 pKa = 11.84IAANAARR285 pKa = 11.84RR286 pKa = 11.84FRR288 pKa = 11.84KK289 pKa = 9.37RR290 pKa = 11.84SPRR293 pKa = 11.84GTCRR297 pKa = 11.84ALRR300 pKa = 11.84AMNN303 pKa = 4.05

Molecular weight: 31.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3671

0

3671

1212999

39

2162

330.4

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.925 ± 0.063

0.465 ± 0.008

6.435 ± 0.04

5.381 ± 0.042

3.115 ± 0.031

8.966 ± 0.04

1.914 ± 0.021

4.261 ± 0.031

1.739 ± 0.027

9.98 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.659 ± 0.015

1.871 ± 0.023

5.6 ± 0.03

2.563 ± 0.021

7.577 ± 0.05

5.658 ± 0.033

6.188 ± 0.038

9.211 ± 0.043

1.568 ± 0.019

1.922 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski