Amino acid dipepetide frequency for Denitrobacterium detoxificans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.928AlaAla: 12.928 ± 0.238
2.068AlaCys: 2.068 ± 0.086
6.003AlaAsp: 6.003 ± 0.132
6.764AlaGlu: 6.764 ± 0.149
4.381AlaPhe: 4.381 ± 0.12
8.934AlaGly: 8.934 ± 0.135
2.271AlaHis: 2.271 ± 0.068
5.973AlaIle: 5.973 ± 0.128
4.199AlaLys: 4.199 ± 0.101
10.788AlaLeu: 10.788 ± 0.213
3.041AlaMet: 3.041 ± 0.088
3.67AlaAsn: 3.67 ± 0.11
4.096AlaPro: 4.096 ± 0.125
4.434AlaGln: 4.434 ± 0.096
5.941AlaArg: 5.941 ± 0.126
7.454AlaSer: 7.454 ± 0.131
6.165AlaThr: 6.165 ± 0.173
7.589AlaVal: 7.589 ± 0.139
1.318AlaTrp: 1.318 ± 0.047
3.495AlaTyr: 3.495 ± 0.078
0.001AlaXaa: 0.001 ± 0.001
Cys
1.817CysAla: 1.817 ± 0.078
0.288CysCys: 0.288 ± 0.023
1.006CysAsp: 1.006 ± 0.041
0.928CysGlu: 0.928 ± 0.043
0.544CysPhe: 0.544 ± 0.034
1.766CysGly: 1.766 ± 0.079
0.327CysHis: 0.327 ± 0.027
0.904CysIle: 0.904 ± 0.046
0.509CysLys: 0.509 ± 0.03
1.219CysLeu: 1.219 ± 0.047
0.456CysMet: 0.456 ± 0.028
0.442CysAsn: 0.442 ± 0.026
0.872CysPro: 0.872 ± 0.051
0.446CysGln: 0.446 ± 0.027
0.709CysArg: 0.709 ± 0.035
1.001CysSer: 1.001 ± 0.037
0.933CysThr: 0.933 ± 0.045
1.351CysVal: 1.351 ± 0.062
0.164CysTrp: 0.164 ± 0.016
0.421CysTyr: 0.421 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
7.464AspAla: 7.464 ± 0.104
0.824AspCys: 0.824 ± 0.038
3.612AspAsp: 3.612 ± 0.103
4.674AspGlu: 4.674 ± 0.104
2.37AspPhe: 2.37 ± 0.058
5.423AspGly: 5.423 ± 0.225
0.943AspHis: 0.943 ± 0.039
3.304AspIle: 3.304 ± 0.062
1.812AspLys: 1.812 ± 0.056
4.503AspLeu: 4.503 ± 0.085
1.799AspMet: 1.799 ± 0.057
1.727AspAsn: 1.727 ± 0.086
2.731AspPro: 2.731 ± 0.07
1.34AspGln: 1.34 ± 0.047
2.541AspArg: 2.541 ± 0.067
3.341AspSer: 3.341 ± 0.088
3.134AspThr: 3.134 ± 0.132
4.757AspVal: 4.757 ± 0.076
0.676AspTrp: 0.676 ± 0.033
2.142AspTyr: 2.142 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
7.169GluAla: 7.169 ± 0.171
0.824GluCys: 0.824 ± 0.038
3.615GluAsp: 3.615 ± 0.076
4.922GluGlu: 4.922 ± 0.132
2.24GluPhe: 2.24 ± 0.06
5.299GluGly: 5.299 ± 0.123
1.438GluHis: 1.438 ± 0.05
3.158GluIle: 3.158 ± 0.087
2.426GluLys: 2.426 ± 0.087
5.829GluLeu: 5.829 ± 0.137
1.698GluMet: 1.698 ± 0.059
2.159GluAsn: 2.159 ± 0.059
2.466GluPro: 2.466 ± 0.072
2.437GluGln: 2.437 ± 0.074
4.424GluArg: 4.424 ± 0.123
3.674GluSer: 3.674 ± 0.083
3.277GluThr: 3.277 ± 0.077
4.259GluVal: 4.259 ± 0.085
0.695GluTrp: 0.695 ± 0.034
2.019GluTyr: 2.019 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
4.24PheAla: 4.24 ± 0.1
0.735PheCys: 0.735 ± 0.042
2.75PheAsp: 2.75 ± 0.078
2.265PheGlu: 2.265 ± 0.063
1.411PhePhe: 1.411 ± 0.059
3.304PheGly: 3.304 ± 0.084
0.619PheHis: 0.619 ± 0.028
1.914PheIle: 1.914 ± 0.069
1.154PheLys: 1.154 ± 0.039
3.073PheLeu: 3.073 ± 0.098
1.048PheMet: 1.048 ± 0.054
1.267PheAsn: 1.267 ± 0.064
1.368PhePro: 1.368 ± 0.051
1.082PheGln: 1.082 ± 0.043
1.613PheArg: 1.613 ± 0.052
2.573PheSer: 2.573 ± 0.071
2.335PheThr: 2.335 ± 0.077
3.072PheVal: 3.072 ± 0.073
0.432PheTrp: 0.432 ± 0.028
1.297PheTyr: 1.297 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
7.926GlyAla: 7.926 ± 0.144
1.576GlyCys: 1.576 ± 0.053
4.479GlyAsp: 4.479 ± 0.1
4.962GlyGlu: 4.962 ± 0.093
3.081GlyPhe: 3.081 ± 0.076
6.245GlyGly: 6.245 ± 0.172
1.438GlyHis: 1.438 ± 0.049
5.043GlyIle: 5.043 ± 0.116
3.667GlyLys: 3.667 ± 0.079
6.705GlyLeu: 6.705 ± 0.112
2.573GlyMet: 2.573 ± 0.072
2.975GlyAsn: 2.975 ± 0.109
1.944GlyPro: 1.944 ± 0.077
2.296GlyGln: 2.296 ± 0.058
3.691GlyArg: 3.691 ± 0.096
5.748GlySer: 5.748 ± 0.13
5.629GlyThr: 5.629 ± 0.303
6.761GlyVal: 6.761 ± 0.109
1.388GlyTrp: 1.388 ± 0.11
3.211GlyTyr: 3.211 ± 0.154
0.0GlyXaa: 0.0 ± 0.0
His
2.299HisAla: 2.299 ± 0.065
0.314HisCys: 0.314 ± 0.024
1.217HisAsp: 1.217 ± 0.046
1.163HisGlu: 1.163 ± 0.047
0.707HisPhe: 0.707 ± 0.034
1.582HisGly: 1.582 ± 0.047
0.429HisHis: 0.429 ± 0.029
1.075HisIle: 1.075 ± 0.042
0.573HisLys: 0.573 ± 0.029
1.397HisLeu: 1.397 ± 0.05
0.511HisMet: 0.511 ± 0.027
0.586HisAsn: 0.586 ± 0.027
1.038HisPro: 1.038 ± 0.041
0.464HisGln: 0.464 ± 0.028
0.977HisArg: 0.977 ± 0.048
0.985HisSer: 0.985 ± 0.039
1.042HisThr: 1.042 ± 0.045
1.68HisVal: 1.68 ± 0.053
0.221HisTrp: 0.221 ± 0.027
0.633HisTyr: 0.633 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.786IleAla: 6.786 ± 0.181
1.031IleCys: 1.031 ± 0.044
3.819IleAsp: 3.819 ± 0.08
3.716IleGlu: 3.716 ± 0.094
1.499IlePhe: 1.499 ± 0.063
4.05IleGly: 4.05 ± 0.089
0.815IleHis: 0.815 ± 0.042
2.844IleIle: 2.844 ± 0.083
1.569IleLys: 1.569 ± 0.053
3.882IleLeu: 3.882 ± 0.106
1.382IleMet: 1.382 ± 0.05
1.558IleAsn: 1.558 ± 0.054
2.543IlePro: 2.543 ± 0.073
1.372IleGln: 1.372 ± 0.043
2.42IleArg: 2.42 ± 0.072
3.467IleSer: 3.467 ± 0.085
3.508IleThr: 3.508 ± 0.139
4.762IleVal: 4.762 ± 0.103
0.523IleTrp: 0.523 ± 0.03
1.365IleTyr: 1.365 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
3.867LysAla: 3.867 ± 0.106
0.44LysCys: 0.44 ± 0.026
1.905LysAsp: 1.905 ± 0.061
2.141LysGlu: 2.141 ± 0.071
0.974LysPhe: 0.974 ± 0.037
2.711LysGly: 2.711 ± 0.062
0.71LysHis: 0.71 ± 0.034
1.676LysIle: 1.676 ± 0.052
1.916LysLys: 1.916 ± 0.082
3.336LysLeu: 3.336 ± 0.073
1.065LysMet: 1.065 ± 0.041
1.336LysAsn: 1.336 ± 0.045
1.666LysPro: 1.666 ± 0.046
1.311LysGln: 1.311 ± 0.05
2.644LysArg: 2.644 ± 0.094
2.117LysSer: 2.117 ± 0.068
2.23LysThr: 2.23 ± 0.058
2.707LysVal: 2.707 ± 0.065
0.408LysTrp: 0.408 ± 0.025
1.075LysTyr: 1.075 ± 0.045
0.0LysXaa: 0.0 ± 0.0
Leu
10.222LeuAla: 10.222 ± 0.211
1.499LeuCys: 1.499 ± 0.065
5.129LeuAsp: 5.129 ± 0.088
5.214LeuGlu: 5.214 ± 0.11
3.65LeuPhe: 3.65 ± 0.115
6.741LeuGly: 6.741 ± 0.142
1.655LeuHis: 1.655 ± 0.05
4.135LeuIle: 4.135 ± 0.101
2.813LeuLys: 2.813 ± 0.064
7.497LeuLeu: 7.497 ± 0.209
2.29LeuMet: 2.29 ± 0.073
2.557LeuAsn: 2.557 ± 0.07
4.129LeuPro: 4.129 ± 0.088
2.617LeuGln: 2.617 ± 0.069
4.663LeuArg: 4.663 ± 0.129
6.346LeuSer: 6.346 ± 0.116
4.722LeuThr: 4.722 ± 0.141
7.209LeuVal: 7.209 ± 0.14
0.763LeuTrp: 0.763 ± 0.034
2.615LeuTyr: 2.615 ± 0.099
0.0LeuXaa: 0.0 ± 0.0
Met
3.076MetAla: 3.076 ± 0.085
0.362MetCys: 0.362 ± 0.026
1.307MetAsp: 1.307 ± 0.05
1.607MetGlu: 1.607 ± 0.056
1.089MetPhe: 1.089 ± 0.159
2.338MetGly: 2.338 ± 0.066
0.514MetHis: 0.514 ± 0.028
1.098MetIle: 1.098 ± 0.041
1.185MetLys: 1.185 ± 0.045
2.609MetLeu: 2.609 ± 0.08
0.681MetMet: 0.681 ± 0.033
1.037MetAsn: 1.037 ± 0.04
1.407MetPro: 1.407 ± 0.047
0.996MetGln: 0.996 ± 0.039
1.79MetArg: 1.79 ± 0.053
1.797MetSer: 1.797 ± 0.059
1.543MetThr: 1.543 ± 0.052
1.881MetVal: 1.881 ± 0.064
0.26MetTrp: 0.26 ± 0.018
0.676MetTyr: 0.676 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
4.002AsnAla: 4.002 ± 0.095
0.541AsnCys: 0.541 ± 0.034
2.072AsnAsp: 2.072 ± 0.066
2.194AsnGlu: 2.194 ± 0.062
0.968AsnPhe: 0.968 ± 0.037
3.101AsnGly: 3.101 ± 0.156
0.562AsnHis: 0.562 ± 0.029
1.676AsnIle: 1.676 ± 0.051
1.091AsnLys: 1.091 ± 0.044
2.813AsnLeu: 2.813 ± 0.082
0.989AsnMet: 0.989 ± 0.059
1.073AsnAsn: 1.073 ± 0.059
2.166AsnPro: 2.166 ± 0.063
0.889AsnGln: 0.889 ± 0.043
1.66AsnArg: 1.66 ± 0.051
1.722AsnSer: 1.722 ± 0.07
1.88AsnThr: 1.88 ± 0.09
2.816AsnVal: 2.816 ± 0.073
0.428AsnTrp: 0.428 ± 0.038
1.136AsnTyr: 1.136 ± 0.064
0.0AsnXaa: 0.0 ± 0.0
Pro
4.634ProAla: 4.634 ± 0.113
0.521ProCys: 0.521 ± 0.03
2.538ProAsp: 2.538 ± 0.064
3.278ProGlu: 3.278 ± 0.077
1.752ProPhe: 1.752 ± 0.067
3.017ProGly: 3.017 ± 0.075
0.908ProHis: 0.908 ± 0.036
2.113ProIle: 2.113 ± 0.065
1.451ProLys: 1.451 ± 0.058
3.598ProLeu: 3.598 ± 0.096
0.901ProMet: 0.901 ± 0.042
1.567ProAsn: 1.567 ± 0.053
0.975ProPro: 0.975 ± 0.041
1.516ProGln: 1.516 ± 0.065
1.938ProArg: 1.938 ± 0.066
2.803ProSer: 2.803 ± 0.07
2.548ProThr: 2.548 ± 0.114
3.408ProVal: 3.408 ± 0.087
0.456ProTrp: 0.456 ± 0.03
1.418ProTyr: 1.418 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
3.969GlnAla: 3.969 ± 0.097
0.356GlnCys: 0.356 ± 0.027
1.513GlnAsp: 1.513 ± 0.043
2.068GlnGlu: 2.068 ± 0.068
1.036GlnPhe: 1.036 ± 0.046
2.552GlnGly: 2.552 ± 0.069
0.555GlnHis: 0.555 ± 0.03
1.62GlnIle: 1.62 ± 0.05
1.318GlnLys: 1.318 ± 0.045
2.819GlnLeu: 2.819 ± 0.069
0.921GlnMet: 0.921 ± 0.04
1.111GlnAsn: 1.111 ± 0.041
1.462GlnPro: 1.462 ± 0.063
1.35GlnGln: 1.35 ± 0.059
1.923GlnArg: 1.923 ± 0.062
1.812GlnSer: 1.812 ± 0.053
1.706GlnThr: 1.706 ± 0.054
2.63GlnVal: 2.63 ± 0.071
0.387GlnTrp: 0.387 ± 0.025
0.899GlnTyr: 0.899 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
5.482ArgAla: 5.482 ± 0.127
0.804ArgCys: 0.804 ± 0.041
3.1ArgAsp: 3.1 ± 0.076
3.997ArgGlu: 3.997 ± 0.106
2.056ArgPhe: 2.056 ± 0.057
3.632ArgGly: 3.632 ± 0.091
1.049ArgHis: 1.049 ± 0.048
3.294ArgIle: 3.294 ± 0.097
2.325ArgLys: 2.325 ± 0.068
4.321ArgLeu: 4.321 ± 0.111
1.733ArgMet: 1.733 ± 0.062
1.85ArgAsn: 1.85 ± 0.05
1.959ArgPro: 1.959 ± 0.073
1.625ArgGln: 1.625 ± 0.054
3.199ArgArg: 3.199 ± 0.108
3.013ArgSer: 3.013 ± 0.073
2.82ArgThr: 2.82 ± 0.071
4.233ArgVal: 4.233 ± 0.097
0.622ArgTrp: 0.622 ± 0.034
1.762ArgTyr: 1.762 ± 0.056
0.0ArgXaa: 0.0 ± 0.0
Ser
6.643SerAla: 6.643 ± 0.122
0.887SerCys: 0.887 ± 0.045
3.941SerAsp: 3.941 ± 0.095
3.801SerGlu: 3.801 ± 0.082
2.743SerPhe: 2.743 ± 0.078
5.946SerGly: 5.946 ± 0.16
1.245SerHis: 1.245 ± 0.042
3.432SerIle: 3.432 ± 0.083
2.194SerLys: 2.194 ± 0.062
5.608SerLeu: 5.608 ± 0.097
1.769SerMet: 1.769 ± 0.061
2.18SerAsn: 2.18 ± 0.077
2.341SerPro: 2.341 ± 0.05
2.159SerGln: 2.159 ± 0.057
3.083SerArg: 3.083 ± 0.077
5.157SerSer: 5.157 ± 0.179
3.856SerThr: 3.856 ± 0.139
5.113SerVal: 5.113 ± 0.093
0.812SerTrp: 0.812 ± 0.036
2.218SerTyr: 2.218 ± 0.082
0.0SerXaa: 0.0 ± 0.0
Thr
5.721ThrAla: 5.721 ± 0.201
0.924ThrCys: 0.924 ± 0.053
3.108ThrAsp: 3.108 ± 0.157
2.93ThrGlu: 2.93 ± 0.061
2.325ThrPhe: 2.325 ± 0.095
5.263ThrGly: 5.263 ± 0.23
1.178ThrHis: 1.178 ± 0.041
3.373ThrIle: 3.373 ± 0.105
1.859ThrLys: 1.859 ± 0.056
5.435ThrLeu: 5.435 ± 0.162
1.339ThrMet: 1.339 ± 0.048
2.014ThrAsn: 2.014 ± 0.115
3.053ThrPro: 3.053 ± 0.108
1.908ThrGln: 1.908 ± 0.053
2.693ThrArg: 2.693 ± 0.06
3.937ThrSer: 3.937 ± 0.198
3.71ThrThr: 3.71 ± 0.294
5.031ThrVal: 5.031 ± 0.179
0.808ThrTrp: 0.808 ± 0.067
2.364ThrTyr: 2.364 ± 0.185
0.0ThrXaa: 0.0 ± 0.0
Val
8.552ValAla: 8.552 ± 0.146
1.376ValCys: 1.376 ± 0.056
4.92ValAsp: 4.92 ± 0.077
4.67ValGlu: 4.67 ± 0.089
3.083ValPhe: 3.083 ± 0.081
5.825ValGly: 5.825 ± 0.133
1.378ValHis: 1.378 ± 0.046
4.273ValIle: 4.273 ± 0.079
2.729ValLys: 2.729 ± 0.074
7.116ValLeu: 7.116 ± 0.13
1.908ValMet: 1.908 ± 0.055
2.708ValAsn: 2.708 ± 0.066
3.52ValPro: 3.52 ± 0.077
2.318ValGln: 2.318 ± 0.066
4.374ValArg: 4.374 ± 0.1
5.56ValSer: 5.56 ± 0.108
5.02ValThr: 5.02 ± 0.224
7.117ValVal: 7.117 ± 0.132
0.844ValTrp: 0.844 ± 0.054
2.522ValTyr: 2.522 ± 0.076
0.0ValXaa: 0.0 ± 0.0
Trp
1.104TrpAla: 1.104 ± 0.046
0.187TrpCys: 0.187 ± 0.017
0.738TrpAsp: 0.738 ± 0.067
0.674TrpGlu: 0.674 ± 0.033
0.495TrpPhe: 0.495 ± 0.04
0.873TrpGly: 0.873 ± 0.036
0.266TrpHis: 0.266 ± 0.023
0.583TrpIle: 0.583 ± 0.03
0.472TrpLys: 0.472 ± 0.031
1.083TrpLeu: 1.083 ± 0.043
0.373TrpMet: 0.373 ± 0.025
0.587TrpAsn: 0.587 ± 0.05
0.33TrpPro: 0.33 ± 0.023
0.442TrpGln: 0.442 ± 0.026
0.72TrpArg: 0.72 ± 0.037
0.667TrpSer: 0.667 ± 0.042
0.636TrpThr: 0.636 ± 0.08
0.871TrpVal: 0.871 ± 0.039
0.19TrpTrp: 0.19 ± 0.029
0.435TrpTyr: 0.435 ± 0.032
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.612TyrAla: 3.612 ± 0.105
0.539TyrCys: 0.539 ± 0.029
2.346TyrAsp: 2.346 ± 0.086
2.061TyrGlu: 2.061 ± 0.051
1.147TyrPhe: 1.147 ± 0.041
2.736TyrGly: 2.736 ± 0.081
0.594TyrHis: 0.594 ± 0.034
1.425TyrIle: 1.425 ± 0.049
0.97TyrLys: 0.97 ± 0.046
2.838TyrLeu: 2.838 ± 0.066
0.776TyrMet: 0.776 ± 0.068
1.375TyrAsn: 1.375 ± 0.068
1.273TyrPro: 1.273 ± 0.051
1.003TyrGln: 1.003 ± 0.045
1.875TyrArg: 1.875 ± 0.057
1.955TyrSer: 1.955 ± 0.063
2.278TyrThr: 2.278 ± 0.198
2.567TyrVal: 2.567 ± 0.089
0.331TyrTrp: 0.331 ± 0.036
1.244TyrTyr: 1.244 ± 0.079
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.003XaaXaa: 0.003 ± 0.003
Statistics based on 1932 proteins (715596 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski