Amino acid dipepetide frequency for Bacteroidales bacterium WCE2004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.164AlaAla: 11.164 ± 0.194
1.299AlaCys: 1.299 ± 0.039
6.192AlaAsp: 6.192 ± 0.095
6.22AlaGlu: 6.22 ± 0.115
3.947AlaPhe: 3.947 ± 0.078
8.099AlaGly: 8.099 ± 0.131
1.588AlaHis: 1.588 ± 0.047
4.635AlaIle: 4.635 ± 0.097
3.862AlaLys: 3.862 ± 0.084
9.462AlaLeu: 9.462 ± 0.124
2.104AlaMet: 2.104 ± 0.052
2.822AlaAsn: 2.822 ± 0.065
3.876AlaPro: 3.876 ± 0.077
3.345AlaGln: 3.345 ± 0.079
5.588AlaArg: 5.588 ± 0.105
5.448AlaSer: 5.448 ± 0.091
4.304AlaThr: 4.304 ± 0.083
6.988AlaVal: 6.988 ± 0.119
1.32AlaTrp: 1.32 ± 0.049
3.04AlaTyr: 3.04 ± 0.062
0.0AlaXaa: 0.0 ± 0.0
Cys
1.248CysAla: 1.248 ± 0.042
0.261CysCys: 0.261 ± 0.021
0.769CysAsp: 0.769 ± 0.032
0.701CysGlu: 0.701 ± 0.03
0.603CysPhe: 0.603 ± 0.028
1.258CysGly: 1.258 ± 0.048
0.278CysHis: 0.278 ± 0.02
0.761CysIle: 0.761 ± 0.033
0.503CysLys: 0.503 ± 0.029
1.073CysLeu: 1.073 ± 0.038
0.204CysMet: 0.204 ± 0.018
0.422CysAsn: 0.422 ± 0.024
0.643CysPro: 0.643 ± 0.03
0.347CysGln: 0.347 ± 0.024
0.744CysArg: 0.744 ± 0.037
0.758CysSer: 0.758 ± 0.036
0.67CysThr: 0.67 ± 0.033
0.85CysVal: 0.85 ± 0.035
0.186CysTrp: 0.186 ± 0.018
0.515CysTyr: 0.515 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
6.224AspAla: 6.224 ± 0.109
0.699AspCys: 0.699 ± 0.035
2.966AspAsp: 2.966 ± 0.066
3.566AspGlu: 3.566 ± 0.081
3.187AspPhe: 3.187 ± 0.06
5.568AspGly: 5.568 ± 0.113
0.987AspHis: 0.987 ± 0.043
3.703AspIle: 3.703 ± 0.082
3.051AspLys: 3.051 ± 0.071
5.329AspLeu: 5.329 ± 0.089
1.245AspMet: 1.245 ± 0.042
2.266AspAsn: 2.266 ± 0.062
3.205AspPro: 3.205 ± 0.067
1.414AspGln: 1.414 ± 0.044
3.606AspArg: 3.606 ± 0.073
3.042AspSer: 3.042 ± 0.077
2.72AspThr: 2.72 ± 0.061
3.968AspVal: 3.968 ± 0.067
1.11AspTrp: 1.11 ± 0.045
2.76AspTyr: 2.76 ± 0.075
0.0AspXaa: 0.0 ± 0.0
Glu
5.901GluAla: 5.901 ± 0.113
0.598GluCys: 0.598 ± 0.028
3.37GluAsp: 3.37 ± 0.08
4.327GluGlu: 4.327 ± 0.096
2.358GluPhe: 2.358 ± 0.06
4.538GluGly: 4.538 ± 0.09
1.096GluHis: 1.096 ± 0.043
3.831GluIle: 3.831 ± 0.074
3.642GluLys: 3.642 ± 0.085
5.516GluLeu: 5.516 ± 0.106
1.864GluMet: 1.864 ± 0.052
2.402GluAsn: 2.402 ± 0.066
2.373GluPro: 2.373 ± 0.07
2.35GluGln: 2.35 ± 0.061
3.848GluArg: 3.848 ± 0.071
2.974GluSer: 2.974 ± 0.078
3.032GluThr: 3.032 ± 0.058
4.079GluVal: 4.079 ± 0.08
0.748GluTrp: 0.748 ± 0.038
2.251GluTyr: 2.251 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.956PheAla: 3.956 ± 0.078
0.696PheCys: 0.696 ± 0.036
3.097PheAsp: 3.097 ± 0.061
2.438PheGlu: 2.438 ± 0.058
2.092PhePhe: 2.092 ± 0.059
3.802PheGly: 3.802 ± 0.091
0.812PheHis: 0.812 ± 0.03
2.219PheIle: 2.219 ± 0.06
1.951PheLys: 1.951 ± 0.056
4.047PheLeu: 4.047 ± 0.08
0.984PheMet: 0.984 ± 0.037
1.858PheAsn: 1.858 ± 0.064
1.937PhePro: 1.937 ± 0.057
1.169PheGln: 1.169 ± 0.04
2.692PheArg: 2.692 ± 0.06
3.12PheSer: 3.12 ± 0.073
2.46PheThr: 2.46 ± 0.061
3.269PheVal: 3.269 ± 0.077
0.657PheTrp: 0.657 ± 0.035
1.679PheTyr: 1.679 ± 0.053
0.0PheXaa: 0.0 ± 0.0
Gly
7.029GlyAla: 7.029 ± 0.119
1.069GlyCys: 1.069 ± 0.038
4.257GlyAsp: 4.257 ± 0.079
4.751GlyGlu: 4.751 ± 0.085
3.613GlyPhe: 3.613 ± 0.07
6.26GlyGly: 6.26 ± 0.132
1.552GlyHis: 1.552 ± 0.049
4.983GlyIle: 4.983 ± 0.081
4.389GlyLys: 4.389 ± 0.093
6.997GlyLeu: 6.997 ± 0.096
2.082GlyMet: 2.082 ± 0.049
2.875GlyAsn: 2.875 ± 0.076
2.255GlyPro: 2.255 ± 0.063
2.607GlyGln: 2.607 ± 0.064
4.82GlyArg: 4.82 ± 0.098
4.588GlySer: 4.588 ± 0.103
4.645GlyThr: 4.645 ± 0.087
5.887GlyVal: 5.887 ± 0.102
1.241GlyTrp: 1.241 ± 0.047
3.334GlyTyr: 3.334 ± 0.08
0.0GlyXaa: 0.0 ± 0.0
His
1.428HisAla: 1.428 ± 0.046
0.287HisCys: 0.287 ± 0.02
0.995HisAsp: 0.995 ± 0.034
0.968HisGlu: 0.968 ± 0.034
1.102HisPhe: 1.102 ± 0.04
1.497HisGly: 1.497 ± 0.052
0.471HisHis: 0.471 ± 0.029
1.223HisIle: 1.223 ± 0.034
0.723HisLys: 0.723 ± 0.03
1.686HisLeu: 1.686 ± 0.053
0.33HisMet: 0.33 ± 0.021
0.726HisAsn: 0.726 ± 0.036
1.16HisPro: 1.16 ± 0.04
0.432HisGln: 0.432 ± 0.025
1.023HisArg: 1.023 ± 0.038
0.955HisSer: 0.955 ± 0.036
0.943HisThr: 0.943 ± 0.034
1.132HisVal: 1.132 ± 0.044
0.301HisTrp: 0.301 ± 0.022
0.852HisTyr: 0.852 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
5.135IleAla: 5.135 ± 0.087
0.86IleCys: 0.86 ± 0.04
3.642IleAsp: 3.642 ± 0.073
3.205IleGlu: 3.205 ± 0.083
2.35IlePhe: 2.35 ± 0.062
4.121IleGly: 4.121 ± 0.083
1.012IleHis: 1.012 ± 0.04
2.869IleIle: 2.869 ± 0.074
2.465IleLys: 2.465 ± 0.064
5.508IleLeu: 5.508 ± 0.104
0.972IleMet: 0.972 ± 0.035
2.001IleAsn: 2.001 ± 0.062
3.017IlePro: 3.017 ± 0.06
1.52IleGln: 1.52 ± 0.049
3.461IleArg: 3.461 ± 0.075
3.554IleSer: 3.554 ± 0.084
2.828IleThr: 2.828 ± 0.065
3.993IleVal: 3.993 ± 0.082
0.664IleTrp: 0.664 ± 0.032
2.005IleTyr: 2.005 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.674LysAla: 4.674 ± 0.098
0.393LysCys: 0.393 ± 0.02
3.102LysAsp: 3.102 ± 0.074
3.303LysGlu: 3.303 ± 0.091
1.752LysPhe: 1.752 ± 0.053
3.674LysGly: 3.674 ± 0.085
0.822LysHis: 0.822 ± 0.036
2.709LysIle: 2.709 ± 0.065
3.076LysLys: 3.076 ± 0.088
3.927LysLeu: 3.927 ± 0.081
1.593LysMet: 1.593 ± 0.049
1.94LysAsn: 1.94 ± 0.048
2.035LysPro: 2.035 ± 0.048
1.494LysGln: 1.494 ± 0.044
2.433LysArg: 2.433 ± 0.067
2.404LysSer: 2.404 ± 0.061
2.64LysThr: 2.64 ± 0.062
3.668LysVal: 3.668 ± 0.089
0.645LysTrp: 0.645 ± 0.029
2.07LysTyr: 2.07 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
9.003LeuAla: 9.003 ± 0.137
1.407LeuCys: 1.407 ± 0.043
5.846LeuAsp: 5.846 ± 0.098
5.332LeuGlu: 5.332 ± 0.086
4.092LeuPhe: 4.092 ± 0.087
6.981LeuGly: 6.981 ± 0.104
1.678LeuHis: 1.678 ± 0.046
4.378LeuIle: 4.378 ± 0.095
4.457LeuLys: 4.457 ± 0.084
9.547LeuLeu: 9.547 ± 0.182
2.253LeuMet: 2.253 ± 0.058
3.147LeuAsn: 3.147 ± 0.064
4.632LeuPro: 4.632 ± 0.091
3.109LeuGln: 3.109 ± 0.061
5.784LeuArg: 5.784 ± 0.1
6.504LeuSer: 6.504 ± 0.101
5.303LeuThr: 5.303 ± 0.086
6.311LeuVal: 6.311 ± 0.119
1.147LeuTrp: 1.147 ± 0.043
3.239LeuTyr: 3.239 ± 0.066
0.0LeuXaa: 0.0 ± 0.0
Met
2.28MetAla: 2.28 ± 0.06
0.232MetCys: 0.232 ± 0.021
1.544MetAsp: 1.544 ± 0.049
1.425MetGlu: 1.425 ± 0.047
0.86MetPhe: 0.86 ± 0.039
1.7MetGly: 1.7 ± 0.053
0.44MetHis: 0.44 ± 0.031
1.261MetIle: 1.261 ± 0.046
1.738MetLys: 1.738 ± 0.047
2.226MetLeu: 2.226 ± 0.055
0.725MetMet: 0.725 ± 0.035
0.998MetAsn: 0.998 ± 0.041
1.091MetPro: 1.091 ± 0.038
0.819MetGln: 0.819 ± 0.035
1.41MetArg: 1.41 ± 0.048
1.429MetSer: 1.429 ± 0.047
1.43MetThr: 1.43 ± 0.04
1.548MetVal: 1.548 ± 0.049
0.222MetTrp: 0.222 ± 0.02
0.726MetTyr: 0.726 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
3.325AsnAla: 3.325 ± 0.076
0.474AsnCys: 0.474 ± 0.029
1.922AsnAsp: 1.922 ± 0.055
1.785AsnGlu: 1.785 ± 0.048
1.73AsnPhe: 1.73 ± 0.056
3.049AsnGly: 3.049 ± 0.088
0.704AsnHis: 0.704 ± 0.032
2.34AsnIle: 2.34 ± 0.058
1.6AsnLys: 1.6 ± 0.056
3.424AsnLeu: 3.424 ± 0.066
0.835AsnMet: 0.835 ± 0.03
1.406AsnAsn: 1.406 ± 0.052
2.165AsnPro: 2.165 ± 0.06
0.965AsnGln: 0.965 ± 0.042
1.988AsnArg: 1.988 ± 0.052
1.88AsnSer: 1.88 ± 0.059
1.925AsnThr: 1.925 ± 0.062
2.585AsnVal: 2.585 ± 0.057
0.561AsnTrp: 0.561 ± 0.03
1.621AsnTyr: 1.621 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
4.925ProAla: 4.925 ± 0.103
0.482ProCys: 0.482 ± 0.027
3.554ProAsp: 3.554 ± 0.072
3.836ProGlu: 3.836 ± 0.081
2.056ProPhe: 2.056 ± 0.057
3.704ProGly: 3.704 ± 0.081
0.703ProHis: 0.703 ± 0.034
1.818ProIle: 1.818 ± 0.049
1.691ProLys: 1.691 ± 0.051
3.707ProLeu: 3.707 ± 0.077
1.071ProMet: 1.071 ± 0.041
1.357ProAsn: 1.357 ± 0.044
0.971ProPro: 0.971 ± 0.04
1.618ProGln: 1.618 ± 0.047
2.03ProArg: 2.03 ± 0.057
2.297ProSer: 2.297 ± 0.056
1.99ProThr: 1.99 ± 0.055
3.9ProVal: 3.9 ± 0.069
0.581ProTrp: 0.581 ± 0.031
1.734ProTyr: 1.734 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
3.047GlnAla: 3.047 ± 0.068
0.249GlnCys: 0.249 ± 0.022
1.781GlnAsp: 1.781 ± 0.052
2.17GlnGlu: 2.17 ± 0.058
1.319GlnPhe: 1.319 ± 0.039
2.11GlnGly: 2.11 ± 0.049
0.569GlnHis: 0.569 ± 0.029
1.874GlnIle: 1.874 ± 0.054
1.676GlnLys: 1.676 ± 0.058
2.937GlnLeu: 2.937 ± 0.066
0.997GlnMet: 0.997 ± 0.034
1.292GlnAsn: 1.292 ± 0.05
1.415GlnPro: 1.415 ± 0.046
1.244GlnGln: 1.244 ± 0.047
1.771GlnArg: 1.771 ± 0.054
1.481GlnSer: 1.481 ± 0.051
1.724GlnThr: 1.724 ± 0.05
2.227GlnVal: 2.227 ± 0.057
0.422GlnTrp: 0.422 ± 0.025
1.258GlnTyr: 1.258 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
4.78ArgAla: 4.78 ± 0.097
0.653ArgCys: 0.653 ± 0.028
3.216ArgAsp: 3.216 ± 0.079
3.978ArgGlu: 3.978 ± 0.093
2.935ArgPhe: 2.935 ± 0.069
3.907ArgGly: 3.907 ± 0.07
1.254ArgHis: 1.254 ± 0.044
3.983ArgIle: 3.983 ± 0.092
3.202ArgLys: 3.202 ± 0.073
5.712ArgLeu: 5.712 ± 0.107
1.686ArgMet: 1.686 ± 0.053
2.126ArgAsn: 2.126 ± 0.056
2.378ArgPro: 2.378 ± 0.061
2.092ArgGln: 2.092 ± 0.061
3.811ArgArg: 3.811 ± 0.094
2.986ArgSer: 2.986 ± 0.061
3.017ArgThr: 3.017 ± 0.07
3.78ArgVal: 3.78 ± 0.07
0.922ArgTrp: 0.922 ± 0.037
2.574ArgTyr: 2.574 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
5.28SerAla: 5.28 ± 0.091
0.839SerCys: 0.839 ± 0.035
3.33SerAsp: 3.33 ± 0.068
3.182SerGlu: 3.182 ± 0.065
2.973SerPhe: 2.973 ± 0.066
5.256SerGly: 5.256 ± 0.108
1.034SerHis: 1.034 ± 0.039
3.199SerIle: 3.199 ± 0.077
2.281SerLys: 2.281 ± 0.059
5.724SerLeu: 5.724 ± 0.103
1.204SerMet: 1.204 ± 0.038
1.914SerAsn: 1.914 ± 0.065
2.427SerPro: 2.427 ± 0.056
1.651SerGln: 1.651 ± 0.052
3.354SerArg: 3.354 ± 0.08
3.511SerSer: 3.511 ± 0.119
2.841SerThr: 2.841 ± 0.062
4.425SerVal: 4.425 ± 0.093
0.975SerTrp: 0.975 ± 0.037
2.372SerTyr: 2.372 ± 0.061
0.0SerXaa: 0.0 ± 0.0
Thr
4.917ThrAla: 4.917 ± 0.096
0.555ThrCys: 0.555 ± 0.028
3.113ThrAsp: 3.113 ± 0.062
2.75ThrGlu: 2.75 ± 0.059
2.458ThrPhe: 2.458 ± 0.057
4.549ThrGly: 4.549 ± 0.075
0.987ThrHis: 0.987 ± 0.041
2.701ThrIle: 2.701 ± 0.075
1.972ThrLys: 1.972 ± 0.056
5.64ThrLeu: 5.64 ± 0.102
1.048ThrMet: 1.048 ± 0.036
1.73ThrAsn: 1.73 ± 0.052
2.898ThrPro: 2.898 ± 0.064
1.446ThrGln: 1.446 ± 0.045
2.754ThrArg: 2.754 ± 0.06
2.68ThrSer: 2.68 ± 0.072
2.721ThrThr: 2.721 ± 0.062
4.714ThrVal: 4.714 ± 0.092
0.711ThrTrp: 0.711 ± 0.033
2.024ThrTyr: 2.024 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
6.604ValAla: 6.604 ± 0.099
0.997ValCys: 0.997 ± 0.035
4.373ValAsp: 4.373 ± 0.086
4.261ValGlu: 4.261 ± 0.08
2.981ValPhe: 2.981 ± 0.064
4.882ValGly: 4.882 ± 0.089
1.2ValHis: 1.2 ± 0.041
3.952ValIle: 3.952 ± 0.084
3.554ValLys: 3.554 ± 0.067
6.872ValLeu: 6.872 ± 0.105
1.617ValMet: 1.617 ± 0.047
2.615ValAsn: 2.615 ± 0.066
3.356ValPro: 3.356 ± 0.072
2.157ValGln: 2.157 ± 0.055
4.693ValArg: 4.693 ± 0.101
4.93ValSer: 4.93 ± 0.097
4.166ValThr: 4.166 ± 0.079
5.317ValVal: 5.317 ± 0.099
0.911ValTrp: 0.911 ± 0.037
2.556ValTyr: 2.556 ± 0.064
0.0ValXaa: 0.0 ± 0.0
Trp
1.076TrpAla: 1.076 ± 0.043
0.195TrpCys: 0.195 ± 0.015
0.838TrpAsp: 0.838 ± 0.038
0.776TrpGlu: 0.776 ± 0.036
0.675TrpPhe: 0.675 ± 0.033
1.04TrpGly: 1.04 ± 0.042
0.294TrpHis: 0.294 ± 0.022
0.748TrpIle: 0.748 ± 0.037
0.717TrpLys: 0.717 ± 0.033
1.324TrpLeu: 1.324 ± 0.049
0.443TrpMet: 0.443 ± 0.025
0.7TrpAsn: 0.7 ± 0.032
0.46TrpPro: 0.46 ± 0.022
0.595TrpGln: 0.595 ± 0.035
0.812TrpArg: 0.812 ± 0.035
0.859TrpSer: 0.859 ± 0.037
0.885TrpThr: 0.885 ± 0.036
0.812TrpVal: 0.812 ± 0.04
0.289TrpTrp: 0.289 ± 0.023
0.567TrpTyr: 0.567 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.309TyrAla: 3.309 ± 0.06
0.551TyrCys: 0.551 ± 0.029
2.709TyrAsp: 2.709 ± 0.07
2.125TyrGlu: 2.125 ± 0.055
1.856TyrPhe: 1.856 ± 0.047
3.238TyrGly: 3.238 ± 0.09
0.755TyrHis: 0.755 ± 0.03
1.994TyrIle: 1.994 ± 0.049
1.803TyrLys: 1.803 ± 0.051
3.495TyrLeu: 3.495 ± 0.067
0.842TyrMet: 0.842 ± 0.032
1.686TyrAsn: 1.686 ± 0.057
1.733TyrPro: 1.733 ± 0.05
1.19TyrGln: 1.19 ± 0.046
2.369TyrArg: 2.369 ± 0.064
2.371TyrSer: 2.371 ± 0.061
2.125TyrThr: 2.125 ± 0.062
2.538TyrVal: 2.538 ± 0.062
0.489TyrTrp: 0.489 ± 0.03
1.72TyrTyr: 1.72 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1930 proteins (724282 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski