Hubei permutotetra-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KHQ0|A0A1L3KHQ0_9VIRU RdRp OS=Hubei permutotetra-like virus 4 OX=1923078 PE=4 SV=1
MM1 pKa = 7.51RR2 pKa = 11.84QFSSRR7 pKa = 11.84GRR9 pKa = 11.84GATNNVNARR18 pKa = 11.84NTSGLSHH25 pKa = 6.47TVYY28 pKa = 10.79KK29 pKa = 10.6KK30 pKa = 10.85DD31 pKa = 3.42FTLTTDD37 pKa = 3.1SFTEE41 pKa = 4.46TVSLEE46 pKa = 4.21DD47 pKa = 3.43GGSYY51 pKa = 10.73RR52 pKa = 11.84LIPSVQTEE60 pKa = 4.52TEE62 pKa = 4.34DD63 pKa = 4.83EE64 pKa = 4.76DD65 pKa = 4.64PLDD68 pKa = 4.37PDD70 pKa = 4.14FEE72 pKa = 4.43GLITNIVKK80 pKa = 10.1PPEE83 pKa = 4.12QYY85 pKa = 11.43SNTAHH90 pKa = 6.86LKK92 pKa = 9.95FYY94 pKa = 9.97PLRR97 pKa = 11.84DD98 pKa = 3.27ILKK101 pKa = 9.3FRR103 pKa = 11.84AVDD106 pKa = 3.91FQLNLHH112 pKa = 6.87LSLEE116 pKa = 4.33IVVDD120 pKa = 3.82YY121 pKa = 8.47PTDD124 pKa = 3.6IEE126 pKa = 4.31ALSILWDD133 pKa = 4.59FSQCIYY139 pKa = 10.37NAQVKK144 pKa = 6.95TRR146 pKa = 11.84RR147 pKa = 11.84EE148 pKa = 3.84RR149 pKa = 11.84VEE151 pKa = 3.62GDD153 pKa = 2.78RR154 pKa = 11.84VLFHH158 pKa = 6.76CVAAGLYY165 pKa = 9.94KK166 pKa = 10.03YY167 pKa = 11.01VPVTRR172 pKa = 11.84PFFKK176 pKa = 10.87LSVEE180 pKa = 4.04WTNTGITSSLAYY192 pKa = 9.39TMKK195 pKa = 9.09TQLYY199 pKa = 9.37AFGLFGDD206 pKa = 4.31ARR208 pKa = 11.84QQEE211 pKa = 4.94GFVAPCAMRR220 pKa = 11.84ILKK223 pKa = 9.66KK224 pKa = 10.38RR225 pKa = 11.84PAVAGLTQFSRR236 pKa = 11.84SRR238 pKa = 11.84AMILGYY244 pKa = 10.69

Molecular weight: 27.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KI05|A0A1L3KI05_9VIRU Capsid protein OS=Hubei permutotetra-like virus 4 OX=1923078 PE=3 SV=1
MM1 pKa = 7.27TRR3 pKa = 11.84RR4 pKa = 11.84TKK6 pKa = 10.52KK7 pKa = 10.6KK8 pKa = 8.01PTTKK12 pKa = 10.01TMEE15 pKa = 4.37KK16 pKa = 10.4ALVAAMTNLTTKK28 pKa = 10.16RR29 pKa = 11.84RR30 pKa = 11.84APRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 9.03GCGAVAEE42 pKa = 4.6GEE44 pKa = 4.19MVLRR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 4.02EE51 pKa = 3.99MLVDD55 pKa = 3.55VTLSANTTEE64 pKa = 4.63SNGSVVLALPNFPWLKK80 pKa = 8.76TVAGSFEE87 pKa = 4.1RR88 pKa = 11.84YY89 pKa = 7.3KK90 pKa = 10.22WKK92 pKa = 10.28RR93 pKa = 11.84LNIHH97 pKa = 5.54WRR99 pKa = 11.84AAGGFNKK106 pKa = 10.33GGLVAVGMDD115 pKa = 3.54WSSQMSSAYY124 pKa = 10.4SKK126 pKa = 9.14PTLTAVTEE134 pKa = 4.21RR135 pKa = 11.84QKK137 pKa = 11.25VLSLTPHH144 pKa = 5.96MSLPISANSISKK156 pKa = 9.21MLGLPVRR163 pKa = 11.84MLNSRR168 pKa = 11.84NWYY171 pKa = 9.59DD172 pKa = 3.17ATKK175 pKa = 10.11TDD177 pKa = 4.21DD178 pKa = 3.52EE179 pKa = 5.0GAVGTLRR186 pKa = 11.84YY187 pKa = 8.9AAKK190 pKa = 10.18CDD192 pKa = 3.41SDD194 pKa = 3.75TTAKK198 pKa = 10.5FIGEE202 pKa = 3.71IWVDD206 pKa = 3.62YY207 pKa = 10.72EE208 pKa = 4.51VVLQGTRR215 pKa = 11.84AA216 pKa = 3.18

Molecular weight: 23.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1580

216

1120

526.7

58.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.608 ± 0.623

0.823 ± 0.157

5.696 ± 0.485

5.696 ± 0.242

2.975 ± 0.998

6.899 ± 0.401

2.089 ± 0.392

3.861 ± 0.329

5.823 ± 0.478

8.671 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.101 ± 0.454

3.354 ± 0.28

5.696 ± 0.792

3.228 ± 0.396

7.025 ± 0.086

6.266 ± 0.46

7.848 ± 0.75

7.595 ± 0.194

1.456 ± 0.285

3.291 ± 0.366
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski