Pseudozyma antarctica (strain T-34) (Yeast) (Candida antarctica)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Moesziomyces; Moesziomyces antarcticus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6640 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9LWP5|M9LWP5_PSEA3 Chloride channel protein OS=Pseudozyma antarctica (strain T-34) OX=1151754 GN=PANT_13d00014 PE=3 SV=1
MM1 pKa = 7.62EE2 pKa = 5.05GFINKK7 pKa = 9.47AKK9 pKa = 10.21EE10 pKa = 3.97FAGSDD15 pKa = 3.39QGKK18 pKa = 9.93DD19 pKa = 3.7LINQFSGNKK28 pKa = 9.23NNNNNNSSSGFGGNNNDD45 pKa = 4.32DD46 pKa = 4.42SYY48 pKa = 12.13GSSNNNSSYY57 pKa = 11.21GSSNNNSSYY66 pKa = 11.17GSNDD70 pKa = 2.87NDD72 pKa = 3.7NNNSSSYY79 pKa = 11.0GSSNNNSSSYY89 pKa = 10.88GSNDD93 pKa = 2.88NDD95 pKa = 3.87NNSSSYY101 pKa = 10.75GSSGRR106 pKa = 11.84RR107 pKa = 11.84NNDD110 pKa = 2.93DD111 pKa = 3.79DD112 pKa = 4.3NSYY115 pKa = 11.63GSGNNNSSSYY125 pKa = 10.9GSSGRR130 pKa = 11.84NNDD133 pKa = 3.11NDD135 pKa = 3.28NSYY138 pKa = 11.64GSGNNNSSSYY148 pKa = 11.43GSGNNNLSSYY158 pKa = 11.31GSGNNNSSYY167 pKa = 11.57GNNNDD172 pKa = 3.21NDD174 pKa = 4.58SYY176 pKa = 11.41GSSGRR181 pKa = 11.84GGNNSSSYY189 pKa = 11.01GGNNDD194 pKa = 3.21NDD196 pKa = 4.42SYY198 pKa = 11.83ASSGRR203 pKa = 11.84NNDD206 pKa = 3.13NDD208 pKa = 4.25SYY210 pKa = 11.29GSSGRR215 pKa = 11.84GGNSGGFGSSNDD227 pKa = 3.28NDD229 pKa = 4.41SYY231 pKa = 11.48GSSGRR236 pKa = 11.84GGNSGGYY243 pKa = 9.83GGSNDD248 pKa = 3.34NDD250 pKa = 4.27SYY252 pKa = 11.53GSSGRR257 pKa = 11.84GGSGYY262 pKa = 11.25GNDD265 pKa = 3.78NSDD268 pKa = 4.55SYY270 pKa = 11.96GSSNNNNNDD279 pKa = 3.27YY280 pKa = 11.48

Molecular weight: 28.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9MHQ1|M9MHQ1_PSEA3 Isoform of M9LS63 Uncharacterized protein (Fragment) OS=Pseudozyma antarctica (strain T-34) OX=1151754 GN=PANT_20c00055 PE=4 SV=1
RR1 pKa = 7.66RR2 pKa = 11.84RR3 pKa = 11.84QRR5 pKa = 11.84QQGRR9 pKa = 11.84QRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84QVRR17 pKa = 11.84QQ18 pKa = 3.09

Molecular weight: 2.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6543

97

6640

4029301

18

6082

606.8

65.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.693 ± 0.032

0.99 ± 0.009

5.879 ± 0.022

5.388 ± 0.025

3.182 ± 0.016

6.773 ± 0.031

2.594 ± 0.014

3.799 ± 0.02

4.178 ± 0.024

8.863 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.921 ± 0.009

2.997 ± 0.016

6.372 ± 0.027

4.204 ± 0.022

6.661 ± 0.027

9.525 ± 0.041

5.721 ± 0.016

5.877 ± 0.021

1.2 ± 0.009

2.182 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski