Potato yellow dwarf nucleorhabdovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5L202|D5L202_9RHAB Putative movement protein OS=Potato yellow dwarf nucleorhabdovirus OX=195060 GN=Y PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 6.02SDD4 pKa = 3.62SATPGQGKK12 pKa = 8.23PAATDD17 pKa = 3.39HH18 pKa = 6.66KK19 pKa = 9.99PPEE22 pKa = 4.42PTPTTAADD30 pKa = 3.48PTKK33 pKa = 10.34QQEE36 pKa = 4.68TKK38 pKa = 10.38EE39 pKa = 4.46SPDD42 pKa = 3.12QHH44 pKa = 7.27GKK46 pKa = 9.83EE47 pKa = 3.9EE48 pKa = 4.16HH49 pKa = 6.02TEE51 pKa = 4.0EE52 pKa = 4.61EE53 pKa = 4.13KK54 pKa = 11.13DD55 pKa = 3.63STIEE59 pKa = 3.62WVYY62 pKa = 10.98PDD64 pKa = 4.24WDD66 pKa = 4.82DD67 pKa = 4.09NDD69 pKa = 3.98SDD71 pKa = 5.03IYY73 pKa = 11.53DD74 pKa = 4.19LLYY77 pKa = 10.75EE78 pKa = 4.69CNHH81 pKa = 6.28GEE83 pKa = 3.95WGNN86 pKa = 3.42

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5L204|D5L204_9RHAB Glycoprotein OS=Potato yellow dwarf nucleorhabdovirus OX=195060 GN=G PE=4 SV=1
MM1 pKa = 8.01ISMIGSRR8 pKa = 11.84RR9 pKa = 11.84PIRR12 pKa = 11.84RR13 pKa = 11.84EE14 pKa = 3.44VYY16 pKa = 9.24LASKK20 pKa = 9.73EE21 pKa = 4.42GEE23 pKa = 3.99VTLQKK28 pKa = 10.61EE29 pKa = 4.1KK30 pKa = 10.47TGILISLNYY39 pKa = 8.74IVKK42 pKa = 9.48IHH44 pKa = 6.96LYY46 pKa = 10.6DD47 pKa = 3.51LVLRR51 pKa = 11.84DD52 pKa = 3.93EE53 pKa = 5.15IEE55 pKa = 4.19NQGISYY61 pKa = 10.12PDD63 pKa = 3.24LFKK66 pKa = 11.23YY67 pKa = 11.02VEE69 pKa = 4.3DD70 pKa = 4.7QIGLPPNNPEE80 pKa = 4.36AIIRR84 pKa = 11.84SPDD87 pKa = 3.29MTNSQVKK94 pKa = 9.17EE95 pKa = 4.2LIMLAKK101 pKa = 9.65LHH103 pKa = 6.64AMHH106 pKa = 6.88TEE108 pKa = 3.8EE109 pKa = 5.43KK110 pKa = 9.53ITVFTQKK117 pKa = 9.56TDD119 pKa = 3.81FSEE122 pKa = 4.28KK123 pKa = 10.3TPTLYY128 pKa = 11.01LKK130 pKa = 10.66VGEE133 pKa = 4.49SILRR137 pKa = 11.84NDD139 pKa = 4.0HH140 pKa = 6.88DD141 pKa = 3.91PHH143 pKa = 7.42VVEE146 pKa = 5.14VIYY149 pKa = 10.6RR150 pKa = 11.84DD151 pKa = 3.73SDD153 pKa = 3.83PLPKK157 pKa = 10.4GEE159 pKa = 4.3YY160 pKa = 8.13QLNMTRR166 pKa = 11.84QYY168 pKa = 11.22KK169 pKa = 10.5GDD171 pKa = 3.58SDD173 pKa = 4.08QIKK176 pKa = 10.71CEE178 pKa = 3.53ITFAMFVRR186 pKa = 11.84TPPRR190 pKa = 11.84NNNHH194 pKa = 4.21VSARR198 pKa = 11.84LNLMVLLSKK207 pKa = 10.91YY208 pKa = 10.87SNEE211 pKa = 3.91LKK213 pKa = 9.69KK214 pKa = 9.28TVSDD218 pKa = 3.84PLKK221 pKa = 10.7NLLKK225 pKa = 10.37RR226 pKa = 11.84KK227 pKa = 9.85SDD229 pKa = 3.29TTIPTKK235 pKa = 10.51TDD237 pKa = 2.79GSKK240 pKa = 10.49RR241 pKa = 11.84AFLPSMLKK249 pKa = 9.32MLSPP253 pKa = 4.61

Molecular weight: 29.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3914

86

1931

559.1

62.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.212 ± 1.103

1.533 ± 0.287

5.825 ± 0.554

5.544 ± 0.799

3.602 ± 0.301

5.8 ± 0.37

1.993 ± 0.324

7.154 ± 0.503

5.442 ± 0.716

9.274 ± 0.786

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.577 ± 0.589

5.263 ± 0.368

5.11 ± 0.636

3.168 ± 0.425

5.314 ± 0.679

8.278 ± 0.976

7.052 ± 0.668

5.953 ± 0.576

1.252 ± 0.312

3.654 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski