Thermophagus xiamenensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinilabiliaceae; Thermophagus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3056 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1X3B3|A0A1I1X3B3_9BACT Glycosyltransferase GT2 family OS=Thermophagus xiamenensis OX=385682 GN=SAMN05444380_10567 PE=4 SV=1
MM1 pKa = 7.86PIKK4 pKa = 10.69SSTGKK9 pKa = 10.58YY10 pKa = 9.99LFPLLLFFITIGSKK24 pKa = 10.76GNNVTLSLTGGLLSEE39 pKa = 4.47GDD41 pKa = 3.5EE42 pKa = 4.32MVISRR47 pKa = 11.84LAGGSVLINRR57 pKa = 11.84THH59 pKa = 7.04EE60 pKa = 4.54IIPAHH65 pKa = 5.55SVKK68 pKa = 10.42AYY70 pKa = 8.72PVRR73 pKa = 11.84SNTTEE78 pKa = 3.7MTGVEE83 pKa = 3.73IAQANEE89 pKa = 4.02GPILSDD95 pKa = 4.23DD96 pKa = 4.79EE97 pKa = 5.18YY98 pKa = 11.32NTDD101 pKa = 3.24QDD103 pKa = 3.55RR104 pKa = 11.84VLNGSSLLDD113 pKa = 3.61NDD115 pKa = 4.02EE116 pKa = 5.01DD117 pKa = 4.6PNGDD121 pKa = 3.43EE122 pKa = 5.59LMINTTPVSPPEE134 pKa = 4.47HH135 pKa = 5.81GTLVINPDD143 pKa = 2.93GTFTYY148 pKa = 10.08TPHH151 pKa = 6.65QGFAGTDD158 pKa = 3.39SFVYY162 pKa = 9.06EE163 pKa = 4.41ACDD166 pKa = 3.43NGDD169 pKa = 3.88PPLCATASVSILVVEE184 pKa = 4.94VADD187 pKa = 4.56HH188 pKa = 7.29DD189 pKa = 4.17NDD191 pKa = 5.08GIPTEE196 pKa = 4.14EE197 pKa = 4.37EE198 pKa = 4.89GYY200 pKa = 10.36IDD202 pKa = 4.28TDD204 pKa = 3.12NDD206 pKa = 4.68GIPNYY211 pKa = 10.34LDD213 pKa = 4.39PDD215 pKa = 3.9SDD217 pKa = 4.0NDD219 pKa = 3.76GMGDD223 pKa = 3.62AEE225 pKa = 5.33EE226 pKa = 4.74GTGDD230 pKa = 4.42CDD232 pKa = 3.75SDD234 pKa = 4.59QIPDD238 pKa = 3.84YY239 pKa = 11.12LDD241 pKa = 3.62TDD243 pKa = 3.77PCFTVPEE250 pKa = 4.69GFSPNDD256 pKa = 3.44DD257 pKa = 4.56GINEE261 pKa = 4.11VLNIRR266 pKa = 11.84WVQLYY271 pKa = 9.07NQVSIMIFNRR281 pKa = 11.84WGSTVFRR288 pKa = 11.84EE289 pKa = 4.45EE290 pKa = 4.14NFSGQWDD297 pKa = 4.03GLSNTGITIGKK308 pKa = 8.74KK309 pKa = 10.15LPAGTYY315 pKa = 9.93YY316 pKa = 10.88YY317 pKa = 9.96IITIGDD323 pKa = 3.6INKK326 pKa = 9.85KK327 pKa = 9.74ISGNIYY333 pKa = 8.47MAWW336 pKa = 3.19

Molecular weight: 36.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2AEW0|A0A1I2AEW0_9BACT Starch-binding associating with outer membrane OS=Thermophagus xiamenensis OX=385682 GN=SAMN05444380_11147 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNRR10 pKa = 11.84KK11 pKa = 8.88RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.98HH16 pKa = 4.03GFRR19 pKa = 11.84SRR21 pKa = 11.84MKK23 pKa = 8.04TATGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.01GRR39 pKa = 11.84KK40 pKa = 8.51KK41 pKa = 9.9LTVSSEE47 pKa = 4.22RR48 pKa = 11.84KK49 pKa = 9.44HH50 pKa = 6.03KK51 pKa = 10.83AA52 pKa = 2.84

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3056

0

3056

1082107

29

5932

354.1

40.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.225 ± 0.042

0.805 ± 0.016

5.453 ± 0.032

6.716 ± 0.039

5.168 ± 0.038

6.791 ± 0.046

1.975 ± 0.022

7.649 ± 0.039

6.884 ± 0.06

9.447 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.022

5.458 ± 0.037

3.994 ± 0.026

3.491 ± 0.025

4.481 ± 0.033

6.314 ± 0.043

5.112 ± 0.052

6.402 ± 0.041

1.285 ± 0.018

3.999 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski