Mus musculus polyomavirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0SYZ9|A0A2S0SYZ9_9POLY VP3 OS=Mus musculus polyomavirus 3 OX=2171394 PE=4 SV=1
MM1 pKa = 7.41GALLAIPEE9 pKa = 4.98LIAAAVEE16 pKa = 4.5GGAEE20 pKa = 4.45AIAIAGSEE28 pKa = 4.26SAILTGEE35 pKa = 4.1GAANLGLLSEE45 pKa = 4.56SAAVLDD51 pKa = 4.35GSIEE55 pKa = 3.9VSAAAEE61 pKa = 4.1SVLVNTPKK69 pKa = 10.36VVPEE73 pKa = 4.84LIAIQGGVQAGAGLTGGIASYY94 pKa = 10.75FLTADD99 pKa = 3.67SPGEE103 pKa = 3.97LPAQQHH109 pKa = 5.03TSGGRR114 pKa = 11.84DD115 pKa = 3.24VQGYY119 pKa = 9.83NGAQGSMALQGIPLHH134 pKa = 6.2ALEE137 pKa = 5.38HH138 pKa = 5.95GVPGIPDD145 pKa = 3.34WVLQLVPDD153 pKa = 4.79LPSLQDD159 pKa = 2.94IVYY162 pKa = 9.28RR163 pKa = 11.84IASGIVSSYY172 pKa = 7.83YY173 pKa = 8.39HH174 pKa = 6.2TGRR177 pKa = 11.84VIVQRR182 pKa = 11.84ALSEE186 pKa = 4.0EE187 pKa = 4.14MQRR190 pKa = 11.84LLNDD194 pKa = 3.43LSDD197 pKa = 3.5GFRR200 pKa = 11.84YY201 pKa = 8.35TFEE204 pKa = 3.97TLGRR208 pKa = 11.84SDD210 pKa = 4.16PVGAMIDD217 pKa = 3.87QIQSAHH223 pKa = 6.31RR224 pKa = 11.84YY225 pKa = 6.61FRR227 pKa = 11.84RR228 pKa = 11.84RR229 pKa = 11.84EE230 pKa = 3.71QMLLIEE236 pKa = 5.1GSDD239 pKa = 3.95PIPFGEE245 pKa = 4.02IASRR249 pKa = 11.84AGEE252 pKa = 3.82IAGNVAEE259 pKa = 4.44TVSNVVVDD267 pKa = 3.95VANLPRR273 pKa = 11.84DD274 pKa = 3.75GYY276 pKa = 10.71NALSEE281 pKa = 4.77GIHH284 pKa = 7.17RR285 pKa = 11.84LGQWVQYY292 pKa = 10.28AGSDD296 pKa = 3.47GGTGHH301 pKa = 6.91YY302 pKa = 7.58STPSWILYY310 pKa = 8.42VLQEE314 pKa = 4.73LEE316 pKa = 4.19RR317 pKa = 11.84EE318 pKa = 4.24LPKK321 pKa = 10.29IPRR324 pKa = 11.84TSLKK328 pKa = 10.7RR329 pKa = 11.84KK330 pKa = 8.91DD331 pKa = 3.4TDD333 pKa = 3.29NVHH336 pKa = 6.36NNNRR340 pKa = 11.84PKK342 pKa = 10.58KK343 pKa = 9.93ARR345 pKa = 11.84ARR347 pKa = 11.84KK348 pKa = 9.31NPSGPKK354 pKa = 9.67ASKK357 pKa = 10.4SNQKK361 pKa = 9.58RR362 pKa = 11.84RR363 pKa = 11.84SGSVRR368 pKa = 11.84RR369 pKa = 11.84RR370 pKa = 11.84STRR373 pKa = 11.84TNNRR377 pKa = 11.84VV378 pKa = 2.83

Molecular weight: 40.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0SZ03|A0A2S0SZ03_9POLY Small t antigen OS=Mus musculus polyomavirus 3 OX=2171394 PE=4 SV=1
MM1 pKa = 7.71ALQGIPLHH9 pKa = 6.2ALEE12 pKa = 5.38HH13 pKa = 5.95GVPGIPDD20 pKa = 3.34WVLQLVPDD28 pKa = 4.79LPSLQDD34 pKa = 2.94IVYY37 pKa = 9.28RR38 pKa = 11.84IASGIVSSYY47 pKa = 7.83YY48 pKa = 8.39HH49 pKa = 6.2TGRR52 pKa = 11.84VIVQRR57 pKa = 11.84ALSEE61 pKa = 4.0EE62 pKa = 4.14MQRR65 pKa = 11.84LLNDD69 pKa = 3.43LSDD72 pKa = 3.5GFRR75 pKa = 11.84YY76 pKa = 8.35TFEE79 pKa = 3.97TLGRR83 pKa = 11.84SDD85 pKa = 4.16PVGAMIDD92 pKa = 3.87QIQSAHH98 pKa = 6.31RR99 pKa = 11.84YY100 pKa = 6.61FRR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84EE105 pKa = 3.71QMLLIEE111 pKa = 5.1GSDD114 pKa = 3.95PIPFGEE120 pKa = 4.02IASRR124 pKa = 11.84AGEE127 pKa = 3.82IAGNVAEE134 pKa = 4.44TVSNVVVDD142 pKa = 3.95VANLPRR148 pKa = 11.84DD149 pKa = 3.75GYY151 pKa = 10.71NALSEE156 pKa = 4.77GIHH159 pKa = 7.17RR160 pKa = 11.84LGQWVQYY167 pKa = 10.28AGSDD171 pKa = 3.47GGTGHH176 pKa = 6.91YY177 pKa = 7.58STPSWILYY185 pKa = 8.42VLQEE189 pKa = 4.73LEE191 pKa = 4.19RR192 pKa = 11.84EE193 pKa = 4.24LPKK196 pKa = 10.29IPRR199 pKa = 11.84TSLKK203 pKa = 10.7RR204 pKa = 11.84KK205 pKa = 8.91DD206 pKa = 3.4TDD208 pKa = 3.29NVHH211 pKa = 6.36NNNRR215 pKa = 11.84PKK217 pKa = 10.58KK218 pKa = 9.93ARR220 pKa = 11.84ARR222 pKa = 11.84KK223 pKa = 9.31NPSGPKK229 pKa = 9.67ASKK232 pKa = 10.4SNQKK236 pKa = 9.58RR237 pKa = 11.84RR238 pKa = 11.84SGSVRR243 pKa = 11.84RR244 pKa = 11.84RR245 pKa = 11.84STRR248 pKa = 11.84TNNRR252 pKa = 11.84VV253 pKa = 2.83

Molecular weight: 28.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1613

173

464

322.6

36.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.51 ± 1.016

2.48 ± 1.06

4.712 ± 0.502

5.952 ± 0.344

3.286 ± 0.788

7.75 ± 1.153

2.48 ± 0.324

5.022 ± 0.491

6.138 ± 1.661

9.361 ± 0.707

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.288

5.084 ± 0.592

5.146 ± 0.769

4.154 ± 0.278

5.828 ± 1.086

6.324 ± 0.966

4.836 ± 0.771

7.688 ± 0.356

1.24 ± 0.316

3.782 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski