Amino acid dipepetide frequency for Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.244AlaAla: 5.244 ± 0.112
0.795AlaCys: 0.795 ± 0.035
4.107AlaAsp: 4.107 ± 0.081
4.703AlaGlu: 4.703 ± 0.097
2.703AlaPhe: 2.703 ± 0.062
5.272AlaGly: 5.272 ± 0.111
1.203AlaHis: 1.203 ± 0.043
6.052AlaIle: 6.052 ± 0.115
4.336AlaLys: 4.336 ± 0.084
6.551AlaLeu: 6.551 ± 0.116
2.119AlaMet: 2.119 ± 0.056
2.669AlaAsn: 2.669 ± 0.064
2.011AlaPro: 2.011 ± 0.063
1.71AlaGln: 1.71 ± 0.051
2.73AlaArg: 2.73 ± 0.074
4.512AlaSer: 4.512 ± 0.089
3.548AlaThr: 3.548 ± 0.084
5.19AlaVal: 5.19 ± 0.107
0.479AlaTrp: 0.479 ± 0.034
2.035AlaTyr: 2.035 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.735CysAla: 0.735 ± 0.033
0.195CysCys: 0.195 ± 0.02
0.78CysAsp: 0.78 ± 0.036
0.801CysGlu: 0.801 ± 0.039
0.395CysPhe: 0.395 ± 0.025
1.295CysGly: 1.295 ± 0.044
0.279CysHis: 0.279 ± 0.025
0.921CysIle: 0.921 ± 0.04
0.693CysLys: 0.693 ± 0.033
0.816CysLeu: 0.816 ± 0.037
0.328CysMet: 0.328 ± 0.024
0.607CysAsn: 0.607 ± 0.04
0.748CysPro: 0.748 ± 0.044
0.263CysGln: 0.263 ± 0.021
0.457CysArg: 0.457 ± 0.028
0.794CysSer: 0.794 ± 0.037
0.634CysThr: 0.634 ± 0.035
0.776CysVal: 0.776 ± 0.036
0.078CysTrp: 0.078 ± 0.011
0.395CysTyr: 0.395 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
4.448AspAla: 4.448 ± 0.096
0.616AspCys: 0.616 ± 0.031
3.653AspAsp: 3.653 ± 0.093
5.121AspGlu: 5.121 ± 0.107
2.465AspPhe: 2.465 ± 0.063
4.142AspGly: 4.142 ± 0.103
1.329AspHis: 1.329 ± 0.045
6.328AspIle: 6.328 ± 0.104
3.602AspLys: 3.602 ± 0.081
5.724AspLeu: 5.724 ± 0.106
2.059AspMet: 2.059 ± 0.056
2.651AspAsn: 2.651 ± 0.072
2.543AspPro: 2.543 ± 0.067
1.292AspGln: 1.292 ± 0.047
2.458AspArg: 2.458 ± 0.065
3.879AspSer: 3.879 ± 0.082
3.121AspThr: 3.121 ± 0.08
5.412AspVal: 5.412 ± 0.111
0.584AspTrp: 0.584 ± 0.032
1.963AspTyr: 1.963 ± 0.058
0.0AspXaa: 0.0 ± 0.0
Glu
4.602GluAla: 4.602 ± 0.085
0.782GluCys: 0.782 ± 0.044
4.617GluAsp: 4.617 ± 0.082
5.499GluGlu: 5.499 ± 0.096
2.761GluPhe: 2.761 ± 0.063
4.638GluGly: 4.638 ± 0.096
1.504GluHis: 1.504 ± 0.052
6.155GluIle: 6.155 ± 0.103
5.631GluLys: 5.631 ± 0.098
7.141GluLeu: 7.141 ± 0.115
2.591GluMet: 2.591 ± 0.063
3.973GluAsn: 3.973 ± 0.084
2.11GluPro: 2.11 ± 0.058
2.057GluGln: 2.057 ± 0.059
3.306GluArg: 3.306 ± 0.08
4.063GluSer: 4.063 ± 0.098
3.476GluThr: 3.476 ± 0.077
5.014GluVal: 5.014 ± 0.106
0.693GluTrp: 0.693 ± 0.037
2.569GluTyr: 2.569 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
2.752PheAla: 2.752 ± 0.075
0.483PheCys: 0.483 ± 0.03
2.799PheAsp: 2.799 ± 0.072
2.979PheGlu: 2.979 ± 0.073
1.609PhePhe: 1.609 ± 0.06
3.006PheGly: 3.006 ± 0.072
0.715PheHis: 0.715 ± 0.035
3.364PheIle: 3.364 ± 0.081
2.224PheLys: 2.224 ± 0.059
3.498PheLeu: 3.498 ± 0.093
1.184PheMet: 1.184 ± 0.045
1.685PheAsn: 1.685 ± 0.047
1.351PhePro: 1.351 ± 0.044
0.85PheGln: 0.85 ± 0.034
1.479PheArg: 1.479 ± 0.046
2.901PheSer: 2.901 ± 0.07
2.393PheThr: 2.393 ± 0.072
3.101PheVal: 3.101 ± 0.069
0.38PheTrp: 0.38 ± 0.022
1.38PheTyr: 1.38 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
4.667GlyAla: 4.667 ± 0.103
1.101GlyCys: 1.101 ± 0.048
4.023GlyAsp: 4.023 ± 0.081
4.386GlyGlu: 4.386 ± 0.107
3.095GlyPhe: 3.095 ± 0.074
4.771GlyGly: 4.771 ± 0.105
1.407GlyHis: 1.407 ± 0.048
6.653GlyIle: 6.653 ± 0.109
4.966GlyLys: 4.966 ± 0.087
6.005GlyLeu: 6.005 ± 0.111
2.407GlyMet: 2.407 ± 0.066
3.268GlyAsn: 3.268 ± 0.09
1.864GlyPro: 1.864 ± 0.054
1.623GlyGln: 1.623 ± 0.051
2.818GlyArg: 2.818 ± 0.067
4.858GlySer: 4.858 ± 0.091
4.315GlyThr: 4.315 ± 0.108
5.22GlyVal: 5.22 ± 0.099
0.665GlyTrp: 0.665 ± 0.037
2.767GlyTyr: 2.767 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.28HisAla: 1.28 ± 0.046
0.285HisCys: 0.285 ± 0.02
1.209HisAsp: 1.209 ± 0.04
1.278HisGlu: 1.278 ± 0.047
0.81HisPhe: 0.81 ± 0.034
1.317HisGly: 1.317 ± 0.047
0.46HisHis: 0.46 ± 0.025
1.642HisIle: 1.642 ± 0.049
1.029HisLys: 1.029 ± 0.042
1.818HisLeu: 1.818 ± 0.054
0.56HisMet: 0.56 ± 0.026
0.847HisAsn: 0.847 ± 0.037
1.067HisPro: 1.067 ± 0.044
0.452HisGln: 0.452 ± 0.024
0.816HisArg: 0.816 ± 0.037
1.308HisSer: 1.308 ± 0.045
0.965HisThr: 0.965 ± 0.041
1.369HisVal: 1.369 ± 0.048
0.174HisTrp: 0.174 ± 0.016
0.665HisTyr: 0.665 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.243IleAla: 6.243 ± 0.101
0.964IleCys: 0.964 ± 0.04
5.804IleAsp: 5.804 ± 0.111
6.546IleGlu: 6.546 ± 0.097
3.37IlePhe: 3.37 ± 0.081
6.296IleGly: 6.296 ± 0.111
1.536IleHis: 1.536 ± 0.044
6.444IleIle: 6.444 ± 0.132
4.923IleLys: 4.923 ± 0.103
7.434IleLeu: 7.434 ± 0.123
2.081IleMet: 2.081 ± 0.057
3.708IleAsn: 3.708 ± 0.103
3.563IlePro: 3.563 ± 0.081
1.781IleGln: 1.781 ± 0.057
3.59IleArg: 3.59 ± 0.07
6.506IleSer: 6.506 ± 0.099
4.754IleThr: 4.754 ± 0.092
5.997IleVal: 5.997 ± 0.091
0.618IleTrp: 0.618 ± 0.033
2.393IleTyr: 2.393 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
4.355LysAla: 4.355 ± 0.089
0.848LysCys: 0.848 ± 0.041
4.187LysAsp: 4.187 ± 0.093
5.374LysGlu: 5.374 ± 0.091
2.084LysPhe: 2.084 ± 0.057
4.395LysGly: 4.395 ± 0.088
1.218LysHis: 1.218 ± 0.046
5.106LysIle: 5.106 ± 0.112
4.893LysLys: 4.893 ± 0.104
5.208LysLeu: 5.208 ± 0.102
2.13LysMet: 2.13 ± 0.059
3.448LysAsn: 3.448 ± 0.091
1.979LysPro: 1.979 ± 0.057
1.765LysGln: 1.765 ± 0.055
3.062LysArg: 3.062 ± 0.066
3.135LysSer: 3.135 ± 0.073
3.25LysThr: 3.25 ± 0.069
4.512LysVal: 4.512 ± 0.089
0.619LysTrp: 0.619 ± 0.033
2.492LysTyr: 2.492 ± 0.072
0.0LysXaa: 0.0 ± 0.0
Leu
6.302LeuAla: 6.302 ± 0.124
1.017LeuCys: 1.017 ± 0.039
5.787LeuAsp: 5.787 ± 0.087
6.807LeuGlu: 6.807 ± 0.126
3.684LeuPhe: 3.684 ± 0.096
6.021LeuGly: 6.021 ± 0.114
1.649LeuHis: 1.649 ± 0.052
6.949LeuIle: 6.949 ± 0.128
6.16LeuLys: 6.16 ± 0.127
8.442LeuLeu: 8.442 ± 0.166
2.574LeuMet: 2.574 ± 0.066
4.135LeuAsn: 4.135 ± 0.089
3.495LeuPro: 3.495 ± 0.07
2.198LeuGln: 2.198 ± 0.064
3.59LeuArg: 3.59 ± 0.082
6.578LeuSer: 6.578 ± 0.109
4.568LeuThr: 4.568 ± 0.098
6.229LeuVal: 6.229 ± 0.117
0.681LeuTrp: 0.681 ± 0.038
2.613LeuTyr: 2.613 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
2.105MetAla: 2.105 ± 0.066
0.353MetCys: 0.353 ± 0.021
2.001MetAsp: 2.001 ± 0.052
2.15MetGlu: 2.15 ± 0.068
1.187MetPhe: 1.187 ± 0.042
2.267MetGly: 2.267 ± 0.061
0.566MetHis: 0.566 ± 0.034
2.328MetIle: 2.328 ± 0.055
2.035MetLys: 2.035 ± 0.055
2.657MetLeu: 2.657 ± 0.063
0.931MetMet: 0.931 ± 0.037
1.472MetAsn: 1.472 ± 0.05
1.108MetPro: 1.108 ± 0.046
0.845MetGln: 0.845 ± 0.04
1.24MetArg: 1.24 ± 0.047
2.013MetSer: 2.013 ± 0.053
1.694MetThr: 1.694 ± 0.05
2.116MetVal: 2.116 ± 0.057
0.248MetTrp: 0.248 ± 0.021
0.773MetTyr: 0.773 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.206AsnAla: 3.206 ± 0.079
0.575AsnCys: 0.575 ± 0.036
2.721AsnAsp: 2.721 ± 0.072
3.293AsnGlu: 3.293 ± 0.072
1.633AsnPhe: 1.633 ± 0.056
3.281AsnGly: 3.281 ± 0.096
0.714AsnHis: 0.714 ± 0.039
4.521AsnIle: 4.521 ± 0.089
2.737AsnLys: 2.737 ± 0.075
3.696AsnLeu: 3.696 ± 0.089
1.366AsnMet: 1.366 ± 0.047
2.381AsnAsn: 2.381 ± 0.098
1.938AsnPro: 1.938 ± 0.057
0.902AsnGln: 0.902 ± 0.04
1.673AsnArg: 1.673 ± 0.054
2.999AsnSer: 2.999 ± 0.077
2.632AsnThr: 2.632 ± 0.094
3.655AsnVal: 3.655 ± 0.078
0.452AsnTrp: 0.452 ± 0.029
1.561AsnTyr: 1.561 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
2.342ProAla: 2.342 ± 0.061
0.434ProCys: 0.434 ± 0.027
2.62ProAsp: 2.62 ± 0.072
3.455ProGlu: 3.455 ± 0.073
1.713ProPhe: 1.713 ± 0.052
2.332ProGly: 2.332 ± 0.067
0.797ProHis: 0.797 ± 0.034
2.786ProIle: 2.786 ± 0.06
1.982ProLys: 1.982 ± 0.061
3.163ProLeu: 3.163 ± 0.059
0.904ProMet: 0.904 ± 0.038
1.4ProAsn: 1.4 ± 0.045
1.154ProPro: 1.154 ± 0.049
0.984ProGln: 0.984 ± 0.037
1.23ProArg: 1.23 ± 0.05
2.393ProSer: 2.393 ± 0.055
1.943ProThr: 1.943 ± 0.06
3.055ProVal: 3.055 ± 0.072
0.273ProTrp: 0.273 ± 0.018
1.286ProTyr: 1.286 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
1.556GlnAla: 1.556 ± 0.052
0.266GlnCys: 0.266 ± 0.021
1.283GlnAsp: 1.283 ± 0.041
1.642GlnGlu: 1.642 ± 0.047
0.995GlnPhe: 0.995 ± 0.04
1.302GlnGly: 1.302 ± 0.048
0.427GlnHis: 0.427 ± 0.026
2.14GlnIle: 2.14 ± 0.055
1.955GlnLys: 1.955 ± 0.066
2.335GlnLeu: 2.335 ± 0.064
0.931GlnMet: 0.931 ± 0.037
1.216GlnAsn: 1.216 ± 0.045
0.737GlnPro: 0.737 ± 0.038
0.873GlnGln: 0.873 ± 0.044
1.176GlnArg: 1.176 ± 0.054
1.377GlnSer: 1.377 ± 0.047
1.258GlnThr: 1.258 ± 0.043
1.574GlnVal: 1.574 ± 0.05
0.279GlnTrp: 0.279 ± 0.02
1.001GlnTyr: 1.001 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
2.583ArgAla: 2.583 ± 0.068
0.505ArgCys: 0.505 ± 0.028
2.644ArgAsp: 2.644 ± 0.062
2.931ArgGlu: 2.931 ± 0.074
1.679ArgPhe: 1.679 ± 0.05
2.588ArgGly: 2.588 ± 0.069
0.776ArgHis: 0.776 ± 0.034
3.426ArgIle: 3.426 ± 0.079
3.007ArgLys: 3.007 ± 0.066
3.46ArgLeu: 3.46 ± 0.084
1.292ArgMet: 1.292 ± 0.047
1.948ArgAsn: 1.948 ± 0.055
1.36ArgPro: 1.36 ± 0.061
1.12ArgGln: 1.12 ± 0.041
1.803ArgArg: 1.803 ± 0.06
3.074ArgSer: 3.074 ± 0.076
2.255ArgThr: 2.255 ± 0.055
2.656ArgVal: 2.656 ± 0.064
0.341ArgTrp: 0.341 ± 0.023
1.633ArgTyr: 1.633 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
4.128SerAla: 4.128 ± 0.085
0.699SerCys: 0.699 ± 0.035
4.216SerAsp: 4.216 ± 0.084
4.601SerGlu: 4.601 ± 0.085
2.852SerPhe: 2.852 ± 0.072
5.406SerGly: 5.406 ± 0.116
1.33SerHis: 1.33 ± 0.038
5.849SerIle: 5.849 ± 0.099
4.148SerLys: 4.148 ± 0.088
5.926SerLeu: 5.926 ± 0.099
2.035SerMet: 2.035 ± 0.065
3.096SerAsn: 3.096 ± 0.081
2.365SerPro: 2.365 ± 0.064
1.648SerGln: 1.648 ± 0.045
2.693SerArg: 2.693 ± 0.059
5.232SerSer: 5.232 ± 0.136
3.748SerThr: 3.748 ± 0.085
4.598SerVal: 4.598 ± 0.084
0.568SerTrp: 0.568 ± 0.036
2.356SerTyr: 2.356 ± 0.06
0.0SerXaa: 0.0 ± 0.0
Thr
3.767ThrAla: 3.767 ± 0.088
0.547ThrCys: 0.547 ± 0.035
3.336ThrAsp: 3.336 ± 0.076
3.451ThrGlu: 3.451 ± 0.075
2.152ThrPhe: 2.152 ± 0.059
4.448ThrGly: 4.448 ± 0.094
1.091ThrHis: 1.091 ± 0.039
4.887ThrIle: 4.887 ± 0.108
3.084ThrLys: 3.084 ± 0.074
4.926ThrLeu: 4.926 ± 0.087
1.4ThrMet: 1.4 ± 0.044
2.273ThrAsn: 2.273 ± 0.065
2.332ThrPro: 2.332 ± 0.068
1.181ThrGln: 1.181 ± 0.038
2.113ThrArg: 2.113 ± 0.056
3.677ThrSer: 3.677 ± 0.08
3.276ThrThr: 3.276 ± 0.084
4.036ThrVal: 4.036 ± 0.096
0.451ThrTrp: 0.451 ± 0.03
1.806ThrTyr: 1.806 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
5.13ValAla: 5.13 ± 0.092
0.984ValCys: 0.984 ± 0.042
4.67ValAsp: 4.67 ± 0.103
5.331ValGlu: 5.331 ± 0.097
3.049ValPhe: 3.049 ± 0.067
5.003ValGly: 5.003 ± 0.111
1.453ValHis: 1.453 ± 0.047
5.87ValIle: 5.87 ± 0.102
4.375ValLys: 4.375 ± 0.083
6.804ValLeu: 6.804 ± 0.132
2.071ValMet: 2.071 ± 0.058
3.028ValAsn: 3.028 ± 0.07
2.903ValPro: 2.903 ± 0.069
1.608ValGln: 1.608 ± 0.048
3.073ValArg: 3.073 ± 0.08
5.127ValSer: 5.127 ± 0.101
4.117ValThr: 4.117 ± 0.083
5.725ValVal: 5.725 ± 0.11
0.585ValTrp: 0.585 ± 0.028
2.28ValTyr: 2.28 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.458TrpAla: 0.458 ± 0.032
0.096TrpCys: 0.096 ± 0.013
0.584TrpAsp: 0.584 ± 0.031
0.584TrpGlu: 0.584 ± 0.034
0.347TrpPhe: 0.347 ± 0.025
0.562TrpGly: 0.562 ± 0.035
0.195TrpHis: 0.195 ± 0.02
0.726TrpIle: 0.726 ± 0.034
0.627TrpLys: 0.627 ± 0.032
0.804TrpLeu: 0.804 ± 0.037
0.284TrpMet: 0.284 ± 0.018
0.488TrpAsn: 0.488 ± 0.032
0.186TrpPro: 0.186 ± 0.017
0.257TrpGln: 0.257 ± 0.021
0.327TrpArg: 0.327 ± 0.026
0.548TrpSer: 0.548 ± 0.033
0.44TrpThr: 0.44 ± 0.028
0.613TrpVal: 0.613 ± 0.03
0.126TrpTrp: 0.126 ± 0.021
0.396TrpTyr: 0.396 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.068TyrAla: 2.068 ± 0.066
0.442TyrCys: 0.442 ± 0.028
2.35TyrAsp: 2.35 ± 0.065
2.251TyrGlu: 2.251 ± 0.065
1.552TyrPhe: 1.552 ± 0.052
2.538TyrGly: 2.538 ± 0.06
0.702TyrHis: 0.702 ± 0.038
2.4TyrIle: 2.4 ± 0.067
1.652TyrLys: 1.652 ± 0.058
3.074TyrLeu: 3.074 ± 0.076
0.842TyrMet: 0.842 ± 0.032
1.64TyrAsn: 1.64 ± 0.058
1.432TyrPro: 1.432 ± 0.044
0.916TyrGln: 0.916 ± 0.035
1.448TyrArg: 1.448 ± 0.044
2.521TyrSer: 2.521 ± 0.062
1.844TyrThr: 1.844 ± 0.056
2.337TyrVal: 2.337 ± 0.06
0.352TyrTrp: 0.352 ± 0.025
1.428TyrTyr: 1.428 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2242 proteins (676646 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski