Clostridium phage Clo-PEP-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6BY31|A0A1L6BY31_9CAUD Uncharacterized protein OS=Clostridium phage Clo-PEP-1 OX=1927016 GN=CloPEP1_0048 PE=4 SV=1
MM1 pKa = 7.34NNKK4 pKa = 9.03KK5 pKa = 9.95FQEE8 pKa = 4.07TLEE11 pKa = 3.99QLKK14 pKa = 10.68EE15 pKa = 3.82EE16 pKa = 4.68LEE18 pKa = 4.21IQGVEE23 pKa = 4.23FNDD26 pKa = 3.68EE27 pKa = 3.98NDD29 pKa = 4.07LIAAVEE35 pKa = 3.94YY36 pKa = 10.18IIEE39 pKa = 4.1YY40 pKa = 9.86DD41 pKa = 3.7YY42 pKa = 11.52HH43 pKa = 6.81PEE45 pKa = 3.92QYY47 pKa = 10.97VEE49 pKa = 4.74DD50 pKa = 4.14TLCNYY55 pKa = 10.46PEE57 pKa = 4.12MFKK60 pKa = 11.08DD61 pKa = 3.66LL62 pKa = 4.02

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6BY46|A0A1L6BY46_9CAUD Nucleotide-binding protein OS=Clostridium phage Clo-PEP-1 OX=1927016 GN=CloPEP1_0066 PE=4 SV=1
MM1 pKa = 7.66CKK3 pKa = 10.43NSVTLKK9 pKa = 10.05RR10 pKa = 11.84KK11 pKa = 8.41YY12 pKa = 10.86GAMFWIMIFLTGGLWVVTIPFKK34 pKa = 10.42KK35 pKa = 10.07HH36 pKa = 5.23KK37 pKa = 10.0VCPVCNSIIKK47 pKa = 10.15

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

15488

41

986

224.5

25.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.288 ± 0.321

1.324 ± 0.145

6.47 ± 0.239

8.058 ± 0.43

3.687 ± 0.222

6.463 ± 0.288

1.601 ± 0.141

7.496 ± 0.247

9.711 ± 0.433

8.465 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.128

5.811 ± 0.246

3.577 ± 0.157

3.364 ± 0.164

3.829 ± 0.219

5.766 ± 0.216

5.146 ± 0.244

6.089 ± 0.214

1.285 ± 0.114

4.229 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski