Amino acid dipepetide frequency for Erwinia phage phiEa116

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.228AlaAla: 7.228 ± 1.109
0.818AlaCys: 0.818 ± 0.248
4.705AlaAsp: 4.705 ± 0.506
6.205AlaGlu: 6.205 ± 0.696
2.591AlaPhe: 2.591 ± 0.445
7.296AlaGly: 7.296 ± 1.04
1.637AlaHis: 1.637 ± 0.437
4.091AlaIle: 4.091 ± 0.468
5.932AlaLys: 5.932 ± 0.813
6.273AlaLeu: 6.273 ± 0.911
2.523AlaMet: 2.523 ± 0.437
3.819AlaAsn: 3.819 ± 0.605
1.909AlaPro: 1.909 ± 0.268
3.069AlaGln: 3.069 ± 0.461
3.069AlaArg: 3.069 ± 0.531
5.182AlaSer: 5.182 ± 0.731
5.592AlaThr: 5.592 ± 0.935
6.001AlaVal: 6.001 ± 0.572
1.091AlaTrp: 1.091 ± 0.314
3.682AlaTyr: 3.682 ± 0.562
0.0AlaXaa: 0.0 ± 0.0
Cys
1.023CysAla: 1.023 ± 0.284
0.068CysCys: 0.068 ± 0.068
0.409CysAsp: 0.409 ± 0.16
0.75CysGlu: 0.75 ± 0.299
0.273CysPhe: 0.273 ± 0.119
0.818CysGly: 0.818 ± 0.274
0.546CysHis: 0.546 ± 0.167
0.409CysIle: 0.409 ± 0.182
0.818CysLys: 0.818 ± 0.306
0.614CysLeu: 0.614 ± 0.196
0.068CysMet: 0.068 ± 0.061
0.409CysAsn: 0.409 ± 0.186
0.341CysPro: 0.341 ± 0.167
0.682CysGln: 0.682 ± 0.2
0.409CysArg: 0.409 ± 0.179
0.75CysSer: 0.75 ± 0.248
0.477CysThr: 0.477 ± 0.209
0.682CysVal: 0.682 ± 0.177
0.0CysTrp: 0.0 ± 0.0
0.341CysTyr: 0.341 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
5.592AspAla: 5.592 ± 0.549
0.409AspCys: 0.409 ± 0.157
3.887AspAsp: 3.887 ± 0.814
5.182AspGlu: 5.182 ± 0.854
2.728AspPhe: 2.728 ± 0.371
5.592AspGly: 5.592 ± 0.707
0.818AspHis: 0.818 ± 0.237
3.409AspIle: 3.409 ± 0.409
3.75AspLys: 3.75 ± 0.463
4.773AspLeu: 4.773 ± 0.603
1.5AspMet: 1.5 ± 0.271
3.137AspAsn: 3.137 ± 0.598
1.977AspPro: 1.977 ± 0.319
1.364AspGln: 1.364 ± 0.244
2.25AspArg: 2.25 ± 0.422
4.978AspSer: 4.978 ± 0.642
4.16AspThr: 4.16 ± 0.521
3.409AspVal: 3.409 ± 0.393
1.296AspTrp: 1.296 ± 0.351
2.796AspTyr: 2.796 ± 0.493
0.0AspXaa: 0.0 ± 0.0
Glu
4.637GluAla: 4.637 ± 0.708
0.341GluCys: 0.341 ± 0.146
3.478GluAsp: 3.478 ± 0.446
4.16GluGlu: 4.16 ± 0.689
3.0GluPhe: 3.0 ± 0.351
4.432GluGly: 4.432 ± 0.483
1.296GluHis: 1.296 ± 0.254
3.478GluIle: 3.478 ± 0.551
4.16GluLys: 4.16 ± 0.682
4.978GluLeu: 4.978 ± 0.614
1.909GluMet: 1.909 ± 0.392
3.409GluAsn: 3.409 ± 0.44
2.046GluPro: 2.046 ± 0.438
2.046GluGln: 2.046 ± 0.356
3.205GluArg: 3.205 ± 0.47
4.364GluSer: 4.364 ± 0.505
3.546GluThr: 3.546 ± 0.561
3.614GluVal: 3.614 ± 0.45
0.682GluTrp: 0.682 ± 0.16
1.705GluTyr: 1.705 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
3.546PheAla: 3.546 ± 0.531
0.273PheCys: 0.273 ± 0.163
1.909PheAsp: 1.909 ± 0.342
2.455PheGlu: 2.455 ± 0.466
1.637PhePhe: 1.637 ± 0.298
3.069PheGly: 3.069 ± 0.482
0.273PheHis: 0.273 ± 0.119
2.046PheIle: 2.046 ± 0.397
2.591PheLys: 2.591 ± 0.444
2.387PheLeu: 2.387 ± 0.423
0.955PheMet: 0.955 ± 0.238
2.114PheAsn: 2.114 ± 0.471
2.046PhePro: 2.046 ± 0.349
1.023PheGln: 1.023 ± 0.276
1.773PheArg: 1.773 ± 0.392
2.523PheSer: 2.523 ± 0.379
3.409PheThr: 3.409 ± 0.543
1.841PheVal: 1.841 ± 0.299
0.409PheTrp: 0.409 ± 0.176
1.159PheTyr: 1.159 ± 0.246
0.0PheXaa: 0.0 ± 0.0
Gly
6.887GlyAla: 6.887 ± 0.666
1.159GlyCys: 1.159 ± 0.247
4.637GlyAsp: 4.637 ± 0.579
3.819GlyGlu: 3.819 ± 0.461
3.546GlyPhe: 3.546 ± 0.585
5.251GlyGly: 5.251 ± 0.958
1.296GlyHis: 1.296 ± 0.353
4.91GlyIle: 4.91 ± 0.503
4.841GlyLys: 4.841 ± 0.575
5.932GlyLeu: 5.932 ± 0.559
1.637GlyMet: 1.637 ± 0.376
3.409GlyAsn: 3.409 ± 0.512
0.273GlyPro: 0.273 ± 0.145
2.864GlyGln: 2.864 ± 0.455
3.069GlyArg: 3.069 ± 0.485
5.728GlySer: 5.728 ± 0.652
5.182GlyThr: 5.182 ± 0.763
6.137GlyVal: 6.137 ± 0.544
1.091GlyTrp: 1.091 ± 0.358
3.75GlyTyr: 3.75 ± 0.451
0.0GlyXaa: 0.0 ± 0.0
His
1.364HisAla: 1.364 ± 0.328
0.136HisCys: 0.136 ± 0.088
1.568HisAsp: 1.568 ± 0.416
1.023HisGlu: 1.023 ± 0.221
0.682HisPhe: 0.682 ± 0.189
1.159HisGly: 1.159 ± 0.335
0.273HisHis: 0.273 ± 0.124
0.818HisIle: 0.818 ± 0.251
1.159HisLys: 1.159 ± 0.286
1.023HisLeu: 1.023 ± 0.281
0.546HisMet: 0.546 ± 0.153
0.682HisAsn: 0.682 ± 0.297
0.477HisPro: 0.477 ± 0.203
0.682HisGln: 0.682 ± 0.201
0.955HisArg: 0.955 ± 0.219
1.159HisSer: 1.159 ± 0.212
1.227HisThr: 1.227 ± 0.282
1.023HisVal: 1.023 ± 0.275
0.068HisTrp: 0.068 ± 0.062
1.023HisTyr: 1.023 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
4.91IleAla: 4.91 ± 0.575
0.75IleCys: 0.75 ± 0.23
3.614IleAsp: 3.614 ± 0.451
4.091IleGlu: 4.091 ± 0.458
1.773IlePhe: 1.773 ± 0.286
4.023IleGly: 4.023 ± 0.425
1.091IleHis: 1.091 ± 0.273
2.932IleIle: 2.932 ± 0.441
4.705IleLys: 4.705 ± 0.6
4.841IleLeu: 4.841 ± 0.801
0.886IleMet: 0.886 ± 0.249
3.614IleAsn: 3.614 ± 0.644
2.182IlePro: 2.182 ± 0.389
2.114IleGln: 2.114 ± 0.413
2.318IleArg: 2.318 ± 0.373
3.955IleSer: 3.955 ± 0.519
4.091IleThr: 4.091 ± 0.585
3.75IleVal: 3.75 ± 0.439
0.477IleTrp: 0.477 ± 0.144
1.977IleTyr: 1.977 ± 0.342
0.0IleXaa: 0.0 ± 0.0
Lys
6.614LysAla: 6.614 ± 0.706
0.682LysCys: 0.682 ± 0.242
3.819LysAsp: 3.819 ± 0.523
3.546LysGlu: 3.546 ± 0.615
2.387LysPhe: 2.387 ± 0.374
5.251LysGly: 5.251 ± 0.603
0.886LysHis: 0.886 ± 0.218
3.409LysIle: 3.409 ± 0.523
3.682LysLys: 3.682 ± 0.5
4.91LysLeu: 4.91 ± 0.519
1.705LysMet: 1.705 ± 0.31
3.682LysAsn: 3.682 ± 0.462
1.841LysPro: 1.841 ± 0.494
1.977LysGln: 1.977 ± 0.327
3.409LysArg: 3.409 ± 0.56
4.637LysSer: 4.637 ± 0.628
4.501LysThr: 4.501 ± 0.487
4.978LysVal: 4.978 ± 0.421
0.75LysTrp: 0.75 ± 0.207
2.659LysTyr: 2.659 ± 0.5
0.0LysXaa: 0.0 ± 0.0
Leu
7.228LeuAla: 7.228 ± 0.629
0.886LeuCys: 0.886 ± 0.326
6.41LeuAsp: 6.41 ± 0.7
4.569LeuGlu: 4.569 ± 0.593
2.455LeuPhe: 2.455 ± 0.396
3.887LeuGly: 3.887 ± 0.703
1.091LeuHis: 1.091 ± 0.235
3.682LeuIle: 3.682 ± 0.456
4.841LeuLys: 4.841 ± 0.67
6.41LeuLeu: 6.41 ± 0.707
2.387LeuMet: 2.387 ± 0.477
4.228LeuAsn: 4.228 ± 0.636
3.409LeuPro: 3.409 ± 0.432
3.069LeuGln: 3.069 ± 0.505
4.296LeuArg: 4.296 ± 0.602
6.001LeuSer: 6.001 ± 0.753
5.864LeuThr: 5.864 ± 0.592
4.841LeuVal: 4.841 ± 0.636
1.091LeuTrp: 1.091 ± 0.212
2.114LeuTyr: 2.114 ± 0.35
0.0LeuXaa: 0.0 ± 0.0
Met
2.659MetAla: 2.659 ± 0.483
0.205MetCys: 0.205 ± 0.101
1.364MetAsp: 1.364 ± 0.341
0.955MetGlu: 0.955 ± 0.299
0.682MetPhe: 0.682 ± 0.236
1.568MetGly: 1.568 ± 0.329
0.273MetHis: 0.273 ± 0.156
1.432MetIle: 1.432 ± 0.265
2.114MetLys: 2.114 ± 0.398
2.25MetLeu: 2.25 ± 0.386
0.682MetMet: 0.682 ± 0.211
1.432MetAsn: 1.432 ± 0.26
0.886MetPro: 0.886 ± 0.223
1.227MetGln: 1.227 ± 0.282
0.955MetArg: 0.955 ± 0.214
2.523MetSer: 2.523 ± 0.379
2.455MetThr: 2.455 ± 0.319
1.159MetVal: 1.159 ± 0.259
0.0MetTrp: 0.0 ± 0.0
1.091MetTyr: 1.091 ± 0.271
0.0MetXaa: 0.0 ± 0.0
Asn
3.75AsnAla: 3.75 ± 0.543
0.341AsnCys: 0.341 ± 0.179
2.182AsnAsp: 2.182 ± 0.409
2.591AsnGlu: 2.591 ± 0.391
1.705AsnPhe: 1.705 ± 0.374
4.16AsnGly: 4.16 ± 0.529
1.023AsnHis: 1.023 ± 0.324
4.023AsnIle: 4.023 ± 0.412
2.591AsnLys: 2.591 ± 0.368
4.773AsnLeu: 4.773 ± 0.435
1.023AsnMet: 1.023 ± 0.273
3.205AsnAsn: 3.205 ± 0.418
3.478AsnPro: 3.478 ± 0.525
1.977AsnGln: 1.977 ± 0.364
2.455AsnArg: 2.455 ± 0.489
3.955AsnSer: 3.955 ± 0.657
3.273AsnThr: 3.273 ± 0.456
3.273AsnVal: 3.273 ± 0.465
0.818AsnTrp: 0.818 ± 0.226
1.773AsnTyr: 1.773 ± 0.34
0.0AsnXaa: 0.0 ± 0.0
Pro
2.728ProAla: 2.728 ± 0.413
0.136ProCys: 0.136 ± 0.089
2.796ProAsp: 2.796 ± 0.402
3.069ProGlu: 3.069 ± 0.494
1.909ProPhe: 1.909 ± 0.425
0.546ProGly: 0.546 ± 0.236
0.75ProHis: 0.75 ± 0.269
1.841ProIle: 1.841 ± 0.277
2.25ProLys: 2.25 ± 0.363
2.523ProLeu: 2.523 ± 0.347
0.818ProMet: 0.818 ± 0.186
2.182ProAsn: 2.182 ± 0.35
0.955ProPro: 0.955 ± 0.219
0.955ProGln: 0.955 ± 0.268
1.227ProArg: 1.227 ± 0.308
2.591ProSer: 2.591 ± 0.378
2.659ProThr: 2.659 ± 0.469
2.523ProVal: 2.523 ± 0.572
0.409ProTrp: 0.409 ± 0.209
0.818ProTyr: 0.818 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
3.0GlnAla: 3.0 ± 0.489
0.273GlnCys: 0.273 ± 0.127
1.364GlnAsp: 1.364 ± 0.299
1.568GlnGlu: 1.568 ± 0.391
1.568GlnPhe: 1.568 ± 0.295
2.523GlnGly: 2.523 ± 0.413
0.546GlnHis: 0.546 ± 0.21
3.0GlnIle: 3.0 ± 0.442
2.455GlnLys: 2.455 ± 0.405
3.409GlnLeu: 3.409 ± 0.422
1.296GlnMet: 1.296 ± 0.318
1.909GlnAsn: 1.909 ± 0.363
1.5GlnPro: 1.5 ± 0.323
1.296GlnGln: 1.296 ± 0.255
1.296GlnArg: 1.296 ± 0.259
2.114GlnSer: 2.114 ± 0.363
2.864GlnThr: 2.864 ± 0.369
1.841GlnVal: 1.841 ± 0.307
0.682GlnTrp: 0.682 ± 0.255
1.773GlnTyr: 1.773 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
3.273ArgAla: 3.273 ± 0.343
0.682ArgCys: 0.682 ± 0.209
2.114ArgAsp: 2.114 ± 0.486
1.977ArgGlu: 1.977 ± 0.486
1.705ArgPhe: 1.705 ± 0.328
2.932ArgGly: 2.932 ± 0.531
1.091ArgHis: 1.091 ± 0.313
3.341ArgIle: 3.341 ± 0.489
3.478ArgLys: 3.478 ± 0.647
3.682ArgLeu: 3.682 ± 0.57
1.637ArgMet: 1.637 ± 0.494
2.114ArgAsn: 2.114 ± 0.414
1.432ArgPro: 1.432 ± 0.209
1.705ArgGln: 1.705 ± 0.279
2.182ArgArg: 2.182 ± 0.495
1.909ArgSer: 1.909 ± 0.327
2.25ArgThr: 2.25 ± 0.413
3.273ArgVal: 3.273 ± 0.452
0.75ArgTrp: 0.75 ± 0.208
2.114ArgTyr: 2.114 ± 0.507
0.0ArgXaa: 0.0 ± 0.0
Ser
5.592SerAla: 5.592 ± 0.82
0.682SerCys: 0.682 ± 0.215
4.569SerAsp: 4.569 ± 0.522
4.091SerGlu: 4.091 ± 0.617
2.591SerPhe: 2.591 ± 0.338
6.955SerGly: 6.955 ± 0.747
1.023SerHis: 1.023 ± 0.258
3.682SerIle: 3.682 ± 0.458
4.16SerLys: 4.16 ± 0.467
5.387SerLeu: 5.387 ± 0.677
2.114SerMet: 2.114 ± 0.409
3.205SerAsn: 3.205 ± 0.463
2.318SerPro: 2.318 ± 0.375
2.796SerGln: 2.796 ± 0.506
2.25SerArg: 2.25 ± 0.31
5.319SerSer: 5.319 ± 0.692
3.546SerThr: 3.546 ± 0.615
5.932SerVal: 5.932 ± 0.668
0.614SerTrp: 0.614 ± 0.195
3.614SerTyr: 3.614 ± 0.547
0.0SerXaa: 0.0 ± 0.0
Thr
5.182ThrAla: 5.182 ± 0.618
0.477ThrCys: 0.477 ± 0.169
5.114ThrAsp: 5.114 ± 0.757
3.069ThrGlu: 3.069 ± 0.415
2.523ThrPhe: 2.523 ± 0.424
7.092ThrGly: 7.092 ± 0.759
1.023ThrHis: 1.023 ± 0.25
4.364ThrIle: 4.364 ± 0.63
3.341ThrLys: 3.341 ± 0.454
5.592ThrLeu: 5.592 ± 0.742
1.159ThrMet: 1.159 ± 0.242
3.409ThrAsn: 3.409 ± 0.65
2.387ThrPro: 2.387 ± 0.364
2.523ThrGln: 2.523 ± 0.378
2.591ThrArg: 2.591 ± 0.362
5.046ThrSer: 5.046 ± 0.576
5.455ThrThr: 5.455 ± 0.953
6.273ThrVal: 6.273 ± 0.822
0.955ThrTrp: 0.955 ± 0.337
2.455ThrTyr: 2.455 ± 0.457
0.0ThrXaa: 0.0 ± 0.0
Val
4.432ValAla: 4.432 ± 0.663
0.818ValCys: 0.818 ± 0.24
4.841ValAsp: 4.841 ± 0.451
4.364ValGlu: 4.364 ± 0.498
1.977ValPhe: 1.977 ± 0.312
4.91ValGly: 4.91 ± 0.538
1.091ValHis: 1.091 ± 0.222
4.432ValIle: 4.432 ± 0.584
4.773ValLys: 4.773 ± 0.475
4.16ValLeu: 4.16 ± 0.522
1.705ValMet: 1.705 ± 0.368
3.682ValAsn: 3.682 ± 0.532
2.659ValPro: 2.659 ± 0.412
2.591ValGln: 2.591 ± 0.368
3.546ValArg: 3.546 ± 0.471
4.637ValSer: 4.637 ± 0.776
5.182ValThr: 5.182 ± 0.645
4.569ValVal: 4.569 ± 0.796
0.818ValTrp: 0.818 ± 0.315
2.455ValTyr: 2.455 ± 0.316
0.0ValXaa: 0.0 ± 0.0
Trp
0.75TrpAla: 0.75 ± 0.165
0.136TrpCys: 0.136 ± 0.095
1.296TrpAsp: 1.296 ± 0.381
0.682TrpGlu: 0.682 ± 0.203
0.409TrpPhe: 0.409 ± 0.143
0.546TrpGly: 0.546 ± 0.272
0.273TrpHis: 0.273 ± 0.141
0.546TrpIle: 0.546 ± 0.335
1.091TrpLys: 1.091 ± 0.28
1.227TrpLeu: 1.227 ± 0.312
0.409TrpMet: 0.409 ± 0.172
0.818TrpAsn: 0.818 ± 0.269
0.273TrpPro: 0.273 ± 0.114
0.341TrpGln: 0.341 ± 0.144
0.341TrpArg: 0.341 ± 0.14
0.75TrpSer: 0.75 ± 0.242
0.955TrpThr: 0.955 ± 0.233
0.886TrpVal: 0.886 ± 0.188
0.341TrpTrp: 0.341 ± 0.169
0.546TrpTyr: 0.546 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.318TyrAla: 2.318 ± 0.363
0.614TyrCys: 0.614 ± 0.211
3.0TyrAsp: 3.0 ± 0.43
2.318TyrGlu: 2.318 ± 0.389
1.296TyrPhe: 1.296 ± 0.235
3.409TyrGly: 3.409 ± 0.438
0.75TyrHis: 0.75 ± 0.199
2.25TyrIle: 2.25 ± 0.556
2.591TyrLys: 2.591 ± 0.382
3.614TyrLeu: 3.614 ± 0.558
0.886TyrMet: 0.886 ± 0.243
1.909TyrAsn: 1.909 ± 0.252
1.296TyrPro: 1.296 ± 0.304
1.977TyrGln: 1.977 ± 0.367
2.046TyrArg: 2.046 ± 0.307
2.318TyrSer: 2.318 ± 0.396
3.341TyrThr: 3.341 ± 0.442
1.637TyrVal: 1.637 ± 0.33
0.273TyrTrp: 0.273 ± 0.126
1.364TyrTyr: 1.364 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (14666 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski