Tortoise microvirus 70

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8WA45|A0A4P8WA45_9VIRU Uncharacterized protein OS=Tortoise microvirus 70 OX=2583177 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84NFNLGSLATWKK13 pKa = 10.4QIAIGEE19 pKa = 4.33VLDD22 pKa = 5.39LEE24 pKa = 4.37MPEE27 pKa = 3.92AGFRR31 pKa = 11.84AVAFDD36 pKa = 3.82IMASDD41 pKa = 4.28TVCVNVVSGEE51 pKa = 4.19DD52 pKa = 3.32YY53 pKa = 10.45WLVGRR58 pKa = 11.84GDD60 pKa = 3.69GEE62 pKa = 4.55LNIKK66 pKa = 9.75FAIDD70 pKa = 3.42RR71 pKa = 11.84PVGVVVVGDD80 pKa = 3.55AATDD84 pKa = 3.43VFIRR88 pKa = 11.84TLVEE92 pKa = 3.76APVIPEE98 pKa = 4.12SVEE101 pKa = 3.63ASYY104 pKa = 8.04TTIEE108 pKa = 4.42PRR110 pKa = 11.84PAGPSDD116 pKa = 3.44DD117 pKa = 4.55LRR119 pKa = 11.84RR120 pKa = 11.84MMNMVRR126 pKa = 11.84LNQMRR131 pKa = 11.84RR132 pKa = 11.84EE133 pKa = 4.0QQLAAEE139 pKa = 4.74RR140 pKa = 11.84EE141 pKa = 4.36AEE143 pKa = 4.08RR144 pKa = 11.84QAMQSQLDD152 pKa = 3.91EE153 pKa = 5.37LKK155 pKa = 10.56SQMPAPVVASPPTAAPAPDD174 pKa = 4.2AAPEE178 pKa = 4.21GQVIEE183 pKa = 4.31

Molecular weight: 19.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8WA45|A0A4P8WA45_9VIRU Uncharacterized protein OS=Tortoise microvirus 70 OX=2583177 PE=4 SV=1
MM1 pKa = 7.39AVDD4 pKa = 3.69IVHH7 pKa = 7.5FGRR10 pKa = 11.84YY11 pKa = 6.99WDD13 pKa = 4.53LSPKK17 pKa = 8.72EE18 pKa = 3.94WAVVGLIGKK27 pKa = 8.44EE28 pKa = 3.76VARR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84NIKK36 pKa = 9.84IVWGGDD42 pKa = 2.62WRR44 pKa = 11.84FWDD47 pKa = 4.01PAHH50 pKa = 7.23WEE52 pKa = 4.09LKK54 pKa = 9.48DD55 pKa = 3.38WKK57 pKa = 10.6ALRR60 pKa = 11.84VPDD63 pKa = 3.82NPLPVTFSQAFRR75 pKa = 11.84KK76 pKa = 9.82ARR78 pKa = 11.84EE79 pKa = 3.83AGALEE84 pKa = 4.22FVWNGKK90 pKa = 9.62RR91 pKa = 11.84YY92 pKa = 6.9NTRR95 pKa = 11.84LAA97 pKa = 3.66

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1595

97

539

319.0

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.091 ± 1.505

0.251 ± 0.133

6.583 ± 0.295

5.768 ± 0.66

4.263 ± 0.519

7.837 ± 0.723

1.881 ± 0.45

4.326 ± 0.561

4.577 ± 0.662

7.21 ± 0.482

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.435

4.451 ± 0.401

5.517 ± 0.459

4.451 ± 0.326

7.9 ± 0.721

5.266 ± 0.384

5.016 ± 1.07

6.959 ± 1.137

2.132 ± 0.455

3.636 ± 0.912
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski