Amino acid dipepetide frequency for Enterococcus phage IME-EFm1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.499AlaAla: 2.499 ± 0.57
0.078AlaCys: 0.078 ± 0.073
2.186AlaAsp: 2.186 ± 0.469
4.216AlaGlu: 4.216 ± 0.5
2.03AlaPhe: 2.03 ± 0.474
2.967AlaGly: 2.967 ± 0.57
1.484AlaHis: 1.484 ± 0.308
4.529AlaIle: 4.529 ± 0.657
4.685AlaLys: 4.685 ± 0.482
5.153AlaLeu: 5.153 ± 0.908
2.03AlaMet: 2.03 ± 0.303
3.748AlaAsn: 3.748 ± 0.515
1.796AlaPro: 1.796 ± 0.381
2.811AlaGln: 2.811 ± 0.398
2.811AlaArg: 2.811 ± 0.489
2.967AlaSer: 2.967 ± 0.42
3.514AlaThr: 3.514 ± 0.496
3.904AlaVal: 3.904 ± 0.479
0.859AlaTrp: 0.859 ± 0.252
2.186AlaTyr: 2.186 ± 0.368
0.0AlaXaa: 0.0 ± 0.0
Cys
0.547CysAla: 0.547 ± 0.213
0.0CysCys: 0.0 ± 0.0
0.39CysAsp: 0.39 ± 0.18
0.234CysGlu: 0.234 ± 0.162
0.312CysPhe: 0.312 ± 0.188
0.312CysGly: 0.312 ± 0.15
0.156CysHis: 0.156 ± 0.125
0.312CysIle: 0.312 ± 0.122
0.39CysLys: 0.39 ± 0.156
0.547CysLeu: 0.547 ± 0.185
0.156CysMet: 0.156 ± 0.14
0.468CysAsn: 0.468 ± 0.244
0.156CysPro: 0.156 ± 0.107
0.156CysGln: 0.156 ± 0.111
0.078CysArg: 0.078 ± 0.083
0.312CysSer: 0.312 ± 0.172
0.078CysThr: 0.078 ± 0.07
0.468CysVal: 0.468 ± 0.169
0.156CysTrp: 0.156 ± 0.098
0.234CysTyr: 0.234 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
3.201AspAla: 3.201 ± 0.552
0.312AspCys: 0.312 ± 0.139
3.201AspAsp: 3.201 ± 0.421
4.216AspGlu: 4.216 ± 0.549
2.811AspPhe: 2.811 ± 0.378
4.451AspGly: 4.451 ± 0.848
0.625AspHis: 0.625 ± 0.229
4.373AspIle: 4.373 ± 0.51
5.544AspLys: 5.544 ± 0.732
5.388AspLeu: 5.388 ± 0.773
1.64AspMet: 1.64 ± 0.342
2.967AspAsn: 2.967 ± 0.514
2.655AspPro: 2.655 ± 0.438
1.015AspGln: 1.015 ± 0.318
2.03AspArg: 2.03 ± 0.309
3.279AspSer: 3.279 ± 0.515
3.67AspThr: 3.67 ± 0.426
3.358AspVal: 3.358 ± 0.531
0.625AspTrp: 0.625 ± 0.204
3.436AspTyr: 3.436 ± 0.532
0.0AspXaa: 0.0 ± 0.0
Glu
3.748GluAla: 3.748 ± 0.537
0.468GluCys: 0.468 ± 0.19
4.216GluAsp: 4.216 ± 0.676
7.886GluGlu: 7.886 ± 1.195
2.733GluPhe: 2.733 ± 0.459
4.919GluGly: 4.919 ± 0.547
0.937GluHis: 0.937 ± 0.193
4.841GluIle: 4.841 ± 0.605
5.7GluLys: 5.7 ± 0.971
9.058GluLeu: 9.058 ± 0.964
1.952GluMet: 1.952 ± 0.476
3.904GluAsn: 3.904 ± 0.703
1.874GluPro: 1.874 ± 0.425
3.123GluGln: 3.123 ± 0.741
2.811GluArg: 2.811 ± 0.564
4.763GluSer: 4.763 ± 0.595
4.607GluThr: 4.607 ± 0.574
4.841GluVal: 4.841 ± 0.552
1.093GluTrp: 1.093 ± 0.228
3.592GluTyr: 3.592 ± 0.564
0.0GluXaa: 0.0 ± 0.0
Phe
1.64PheAla: 1.64 ± 0.391
0.234PheCys: 0.234 ± 0.16
3.045PheAsp: 3.045 ± 0.49
2.733PheGlu: 2.733 ± 0.466
0.39PhePhe: 0.39 ± 0.17
2.264PheGly: 2.264 ± 0.404
0.625PheHis: 0.625 ± 0.292
3.279PheIle: 3.279 ± 0.452
3.592PheLys: 3.592 ± 0.543
1.796PheLeu: 1.796 ± 0.417
1.484PheMet: 1.484 ± 0.323
2.733PheAsn: 2.733 ± 0.431
0.468PhePro: 0.468 ± 0.175
1.249PheGln: 1.249 ± 0.299
1.249PheArg: 1.249 ± 0.267
2.655PheSer: 2.655 ± 0.458
2.733PheThr: 2.733 ± 0.418
2.421PheVal: 2.421 ± 0.384
0.312PheTrp: 0.312 ± 0.12
1.64PheTyr: 1.64 ± 0.503
0.0PheXaa: 0.0 ± 0.0
Gly
4.216GlyAla: 4.216 ± 0.752
0.39GlyCys: 0.39 ± 0.188
3.358GlyAsp: 3.358 ± 0.746
3.67GlyGlu: 3.67 ± 0.423
2.811GlyPhe: 2.811 ± 0.402
4.295GlyGly: 4.295 ± 0.953
0.703GlyHis: 0.703 ± 0.238
3.67GlyIle: 3.67 ± 0.541
6.715GlyLys: 6.715 ± 0.689
6.09GlyLeu: 6.09 ± 0.866
1.015GlyMet: 1.015 ± 0.206
4.607GlyAsn: 4.607 ± 0.678
0.547GlyPro: 0.547 ± 0.175
2.499GlyGln: 2.499 ± 0.682
2.577GlyArg: 2.577 ± 0.398
3.67GlySer: 3.67 ± 0.64
3.514GlyThr: 3.514 ± 0.594
4.763GlyVal: 4.763 ± 0.829
1.327GlyTrp: 1.327 ± 0.389
3.826GlyTyr: 3.826 ± 0.553
0.0GlyXaa: 0.0 ± 0.0
His
0.781HisAla: 0.781 ± 0.301
0.078HisCys: 0.078 ± 0.105
0.937HisAsp: 0.937 ± 0.342
0.781HisGlu: 0.781 ± 0.242
0.468HisPhe: 0.468 ± 0.207
1.562HisGly: 1.562 ± 0.354
0.312HisHis: 0.312 ± 0.135
1.64HisIle: 1.64 ± 0.271
1.015HisLys: 1.015 ± 0.334
0.703HisLeu: 0.703 ± 0.206
0.234HisMet: 0.234 ± 0.118
1.171HisAsn: 1.171 ± 0.239
0.312HisPro: 0.312 ± 0.139
0.859HisGln: 0.859 ± 0.258
0.39HisArg: 0.39 ± 0.177
0.703HisSer: 0.703 ± 0.244
1.171HisThr: 1.171 ± 0.3
1.093HisVal: 1.093 ± 0.229
0.312HisTrp: 0.312 ± 0.162
0.937HisTyr: 0.937 ± 0.194
0.0HisXaa: 0.0 ± 0.0
Ile
4.451IleAla: 4.451 ± 0.552
0.39IleCys: 0.39 ± 0.204
5.075IleAsp: 5.075 ± 0.592
5.622IleGlu: 5.622 ± 0.561
1.796IlePhe: 1.796 ± 0.399
4.138IleGly: 4.138 ± 0.519
0.937IleHis: 0.937 ± 0.247
4.919IleIle: 4.919 ± 0.451
6.325IleLys: 6.325 ± 0.749
4.216IleLeu: 4.216 ± 0.64
1.64IleMet: 1.64 ± 0.265
5.232IleAsn: 5.232 ± 0.576
2.733IlePro: 2.733 ± 0.488
2.342IleGln: 2.342 ± 0.399
1.952IleArg: 1.952 ± 0.401
3.826IleSer: 3.826 ± 0.669
4.295IleThr: 4.295 ± 0.586
2.655IleVal: 2.655 ± 0.444
0.781IleTrp: 0.781 ± 0.23
2.655IleTyr: 2.655 ± 0.374
0.0IleXaa: 0.0 ± 0.0
Lys
5.466LysAla: 5.466 ± 0.763
0.312LysCys: 0.312 ± 0.176
5.232LysAsp: 5.232 ± 0.47
9.058LysGlu: 9.058 ± 1.252
2.655LysPhe: 2.655 ± 0.376
4.997LysGly: 4.997 ± 0.604
0.703LysHis: 0.703 ± 0.254
4.373LysIle: 4.373 ± 0.592
6.715LysLys: 6.715 ± 0.985
8.589LysLeu: 8.589 ± 0.781
3.045LysMet: 3.045 ± 0.551
4.997LysAsn: 4.997 ± 0.721
2.499LysPro: 2.499 ± 0.434
4.138LysGln: 4.138 ± 0.841
3.904LysArg: 3.904 ± 0.602
4.216LysSer: 4.216 ± 0.579
6.403LysThr: 6.403 ± 0.785
5.778LysVal: 5.778 ± 0.595
1.015LysTrp: 1.015 ± 0.31
4.138LysTyr: 4.138 ± 0.443
0.0LysXaa: 0.0 ± 0.0
Leu
4.919LeuAla: 4.919 ± 0.847
0.39LeuCys: 0.39 ± 0.207
6.247LeuAsp: 6.247 ± 0.688
7.652LeuGlu: 7.652 ± 1.061
2.967LeuPhe: 2.967 ± 0.465
5.856LeuGly: 5.856 ± 0.843
0.703LeuHis: 0.703 ± 0.239
4.763LeuIle: 4.763 ± 0.751
7.808LeuLys: 7.808 ± 0.83
7.496LeuLeu: 7.496 ± 0.792
1.874LeuMet: 1.874 ± 0.397
5.622LeuAsn: 5.622 ± 0.632
3.045LeuPro: 3.045 ± 0.453
3.358LeuGln: 3.358 ± 0.514
3.045LeuArg: 3.045 ± 0.422
5.622LeuSer: 5.622 ± 0.706
6.169LeuThr: 6.169 ± 0.596
5.075LeuVal: 5.075 ± 0.74
0.625LeuTrp: 0.625 ± 0.251
3.748LeuTyr: 3.748 ± 0.649
0.0LeuXaa: 0.0 ± 0.0
Met
1.249MetAla: 1.249 ± 0.242
0.078MetCys: 0.078 ± 0.068
0.937MetAsp: 0.937 ± 0.264
1.405MetGlu: 1.405 ± 0.296
1.015MetPhe: 1.015 ± 0.256
1.015MetGly: 1.015 ± 0.259
0.39MetHis: 0.39 ± 0.198
2.342MetIle: 2.342 ± 0.404
4.216MetLys: 4.216 ± 0.642
1.874MetLeu: 1.874 ± 0.448
1.171MetMet: 1.171 ± 0.353
2.03MetAsn: 2.03 ± 0.431
1.015MetPro: 1.015 ± 0.266
1.327MetGln: 1.327 ± 0.304
0.937MetArg: 0.937 ± 0.255
1.405MetSer: 1.405 ± 0.278
1.718MetThr: 1.718 ± 0.366
1.249MetVal: 1.249 ± 0.215
0.312MetTrp: 0.312 ± 0.192
1.093MetTyr: 1.093 ± 0.343
0.0MetXaa: 0.0 ± 0.0
Asn
3.748AsnAla: 3.748 ± 0.571
0.078AsnCys: 0.078 ± 0.078
2.967AsnAsp: 2.967 ± 0.506
5.153AsnGlu: 5.153 ± 0.565
2.264AsnPhe: 2.264 ± 0.407
5.31AsnGly: 5.31 ± 0.997
1.405AsnHis: 1.405 ± 0.375
3.748AsnIle: 3.748 ± 0.543
7.105AsnLys: 7.105 ± 0.899
4.841AsnLeu: 4.841 ± 0.609
1.874AsnMet: 1.874 ± 0.419
4.529AsnAsn: 4.529 ± 0.784
2.499AsnPro: 2.499 ± 0.531
2.264AsnGln: 2.264 ± 0.386
2.733AsnArg: 2.733 ± 0.443
3.748AsnSer: 3.748 ± 0.518
4.373AsnThr: 4.373 ± 0.704
3.67AsnVal: 3.67 ± 0.415
0.937AsnTrp: 0.937 ± 0.254
3.358AsnTyr: 3.358 ± 0.485
0.0AsnXaa: 0.0 ± 0.0
Pro
1.718ProAla: 1.718 ± 0.359
0.156ProCys: 0.156 ± 0.114
1.952ProAsp: 1.952 ± 0.324
2.733ProGlu: 2.733 ± 0.524
1.64ProPhe: 1.64 ± 0.287
0.703ProGly: 0.703 ± 0.401
0.312ProHis: 0.312 ± 0.141
2.03ProIle: 2.03 ± 0.324
2.577ProLys: 2.577 ± 0.475
2.342ProLeu: 2.342 ± 0.377
0.468ProMet: 0.468 ± 0.171
2.03ProAsn: 2.03 ± 0.441
1.093ProPro: 1.093 ± 0.322
1.484ProGln: 1.484 ± 0.295
0.625ProArg: 0.625 ± 0.224
2.108ProSer: 2.108 ± 0.696
2.186ProThr: 2.186 ± 0.467
1.562ProVal: 1.562 ± 0.426
0.312ProTrp: 0.312 ± 0.133
1.952ProTyr: 1.952 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
2.499GlnAla: 2.499 ± 0.457
0.39GlnCys: 0.39 ± 0.181
2.186GlnAsp: 2.186 ± 0.315
2.264GlnGlu: 2.264 ± 0.575
1.64GlnPhe: 1.64 ± 0.293
2.108GlnGly: 2.108 ± 0.491
0.468GlnHis: 0.468 ± 0.201
2.577GlnIle: 2.577 ± 0.393
2.108GlnLys: 2.108 ± 0.395
4.373GlnLeu: 4.373 ± 0.551
1.093GlnMet: 1.093 ± 0.363
2.108GlnAsn: 2.108 ± 0.36
1.327GlnPro: 1.327 ± 0.468
1.796GlnGln: 1.796 ± 0.432
1.64GlnArg: 1.64 ± 0.363
1.874GlnSer: 1.874 ± 0.402
2.03GlnThr: 2.03 ± 0.357
3.123GlnVal: 3.123 ± 0.548
0.547GlnTrp: 0.547 ± 0.194
1.796GlnTyr: 1.796 ± 0.429
0.0GlnXaa: 0.0 ± 0.0
Arg
1.718ArgAla: 1.718 ± 0.402
0.234ArgCys: 0.234 ± 0.162
2.342ArgAsp: 2.342 ± 0.487
2.577ArgGlu: 2.577 ± 0.453
2.108ArgPhe: 2.108 ± 0.465
2.342ArgGly: 2.342 ± 0.377
1.093ArgHis: 1.093 ± 0.331
2.499ArgIle: 2.499 ± 0.551
2.811ArgLys: 2.811 ± 0.579
3.201ArgLeu: 3.201 ± 0.572
1.171ArgMet: 1.171 ± 0.297
2.499ArgAsn: 2.499 ± 0.334
1.249ArgPro: 1.249 ± 0.267
1.484ArgGln: 1.484 ± 0.348
1.718ArgArg: 1.718 ± 0.335
1.718ArgSer: 1.718 ± 0.359
1.718ArgThr: 1.718 ± 0.291
2.264ArgVal: 2.264 ± 0.338
0.547ArgTrp: 0.547 ± 0.182
2.342ArgTyr: 2.342 ± 0.481
0.0ArgXaa: 0.0 ± 0.0
Ser
2.967SerAla: 2.967 ± 0.647
0.234SerCys: 0.234 ± 0.151
3.279SerAsp: 3.279 ± 0.469
3.67SerGlu: 3.67 ± 0.561
2.342SerPhe: 2.342 ± 0.41
4.919SerGly: 4.919 ± 0.832
1.405SerHis: 1.405 ± 0.333
3.045SerIle: 3.045 ± 0.349
5.7SerLys: 5.7 ± 1.047
5.7SerLeu: 5.7 ± 0.526
1.327SerMet: 1.327 ± 0.267
4.138SerAsn: 4.138 ± 0.753
1.327SerPro: 1.327 ± 0.293
1.796SerGln: 1.796 ± 0.322
1.64SerArg: 1.64 ± 0.314
3.201SerSer: 3.201 ± 0.728
4.763SerThr: 4.763 ± 1.317
4.138SerVal: 4.138 ± 0.612
0.859SerTrp: 0.859 ± 0.316
1.952SerTyr: 1.952 ± 0.477
0.0SerXaa: 0.0 ± 0.0
Thr
3.67ThrAla: 3.67 ± 0.588
0.156ThrCys: 0.156 ± 0.144
2.967ThrAsp: 2.967 ± 0.672
4.138ThrGlu: 4.138 ± 0.499
2.186ThrPhe: 2.186 ± 0.389
5.622ThrGly: 5.622 ± 0.957
1.171ThrHis: 1.171 ± 0.339
5.075ThrIle: 5.075 ± 0.726
5.934ThrLys: 5.934 ± 0.519
4.529ThrLeu: 4.529 ± 0.642
1.405ThrMet: 1.405 ± 0.347
5.232ThrAsn: 5.232 ± 0.668
2.499ThrPro: 2.499 ± 0.527
2.342ThrGln: 2.342 ± 0.471
2.03ThrArg: 2.03 ± 0.355
3.982ThrSer: 3.982 ± 0.699
4.919ThrThr: 4.919 ± 0.883
3.904ThrVal: 3.904 ± 0.667
1.015ThrTrp: 1.015 ± 0.27
2.889ThrTyr: 2.889 ± 0.596
0.0ThrXaa: 0.0 ± 0.0
Val
3.67ValAla: 3.67 ± 0.555
0.781ValCys: 0.781 ± 0.25
4.295ValAsp: 4.295 ± 0.467
4.373ValGlu: 4.373 ± 0.573
2.108ValPhe: 2.108 ± 0.439
4.06ValGly: 4.06 ± 0.808
0.859ValHis: 0.859 ± 0.216
3.904ValIle: 3.904 ± 0.524
4.763ValLys: 4.763 ± 0.605
5.466ValLeu: 5.466 ± 0.703
1.64ValMet: 1.64 ± 0.3
4.373ValAsn: 4.373 ± 0.835
1.718ValPro: 1.718 ± 0.371
1.327ValGln: 1.327 ± 0.27
3.358ValArg: 3.358 ± 0.488
4.529ValSer: 4.529 ± 0.763
4.06ValThr: 4.06 ± 0.595
4.216ValVal: 4.216 ± 0.642
0.937ValTrp: 0.937 ± 0.393
2.03ValTyr: 2.03 ± 0.391
0.0ValXaa: 0.0 ± 0.0
Trp
0.547TrpAla: 0.547 ± 0.225
0.078TrpCys: 0.078 ± 0.09
0.937TrpAsp: 0.937 ± 0.279
1.015TrpGlu: 1.015 ± 0.277
0.547TrpPhe: 0.547 ± 0.156
0.547TrpGly: 0.547 ± 0.216
0.234TrpHis: 0.234 ± 0.122
0.781TrpIle: 0.781 ± 0.303
0.937TrpLys: 0.937 ± 0.228
1.718TrpLeu: 1.718 ± 0.311
0.234TrpMet: 0.234 ± 0.129
0.625TrpAsn: 0.625 ± 0.223
0.078TrpPro: 0.078 ± 0.068
0.547TrpGln: 0.547 ± 0.162
0.625TrpArg: 0.625 ± 0.205
0.859TrpSer: 0.859 ± 0.267
0.937TrpThr: 0.937 ± 0.485
1.171TrpVal: 1.171 ± 0.29
0.0TrpTrp: 0.0 ± 0.0
0.312TrpTyr: 0.312 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.201TyrAla: 3.201 ± 0.457
0.625TyrCys: 0.625 ± 0.253
3.045TyrAsp: 3.045 ± 0.413
3.67TyrGlu: 3.67 ± 0.569
1.718TyrPhe: 1.718 ± 0.308
1.874TyrGly: 1.874 ± 0.376
0.937TyrHis: 0.937 ± 0.27
3.279TyrIle: 3.279 ± 0.479
3.592TyrLys: 3.592 ± 0.608
3.904TyrLeu: 3.904 ± 0.663
1.249TyrMet: 1.249 ± 0.366
3.592TyrAsn: 3.592 ± 0.605
1.093TyrPro: 1.093 ± 0.234
2.03TyrGln: 2.03 ± 0.398
1.562TyrArg: 1.562 ± 0.349
2.967TyrSer: 2.967 ± 0.57
2.811TyrThr: 2.811 ± 0.623
2.811TyrVal: 2.811 ± 0.48
0.156TyrTrp: 0.156 ± 0.122
2.421TyrTyr: 2.421 ± 0.455
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (12808 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski