Williamsia sp. Leaf354

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Williamsia; unclassified Williamsia

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5QLJ4|A0A0Q5QLJ4_9NOCA Peptide-binding protein OS=Williamsia sp. Leaf354 OX=1736349 GN=ASG12_08420 PE=4 SV=1
MM1 pKa = 7.68ICPIDD6 pKa = 4.17GDD8 pKa = 3.95NCTFDD13 pKa = 4.79ADD15 pKa = 3.65KK16 pKa = 11.37CPFDD20 pKa = 4.72GDD22 pKa = 3.93KK23 pKa = 11.33CPMDD27 pKa = 4.27GDD29 pKa = 4.2KK30 pKa = 11.35CPMDD34 pKa = 4.27GDD36 pKa = 4.2KK37 pKa = 11.35CPMDD41 pKa = 4.19GDD43 pKa = 3.87KK44 pKa = 11.39CPFDD48 pKa = 4.52GDD50 pKa = 3.46KK51 pKa = 11.26CPIDD55 pKa = 3.85GASISDD61 pKa = 3.48

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5QQF7|A0A0Q5QQF7_9NOCA TetR family transcriptional regulator OS=Williamsia sp. Leaf354 OX=1736349 GN=ASG12_18070 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVNGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.79GRR42 pKa = 11.84AGLTAA47 pKa = 4.67

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4063

0

4063

1352199

32

13274

332.8

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.201 ± 0.058

0.713 ± 0.013

6.935 ± 0.048

4.808 ± 0.035

2.938 ± 0.022

9.085 ± 0.055

2.195 ± 0.02

4.459 ± 0.03

1.854 ± 0.036

9.341 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.976 ± 0.017

1.931 ± 0.028

5.529 ± 0.034

2.5 ± 0.022

7.439 ± 0.044

5.86 ± 0.03

6.728 ± 0.031

9.232 ± 0.047

1.382 ± 0.015

1.895 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski