Sphingobacterium psychroaquaticum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7J6M4|A0A1X7J6M4_9SPHI Uncharacterized protein OS=Sphingobacterium psychroaquaticum OX=561061 GN=SAMN05660862_1523 PE=4 SV=1
MM1 pKa = 7.63ANWCSNTVVFEE12 pKa = 4.06GTPEE16 pKa = 5.05AITAIQEE23 pKa = 4.26LFQSMKK29 pKa = 10.29EE30 pKa = 4.06KK31 pKa = 10.77EE32 pKa = 4.28EE33 pKa = 4.0KK34 pKa = 9.99TEE36 pKa = 3.96EE37 pKa = 4.24GQLPEE42 pKa = 5.18FMEE45 pKa = 4.64DD46 pKa = 3.14TNGGYY51 pKa = 10.34FFNIYY56 pKa = 9.05WNEE59 pKa = 3.59GDD61 pKa = 3.89EE62 pKa = 4.68GQFQYY67 pKa = 7.99EE68 pKa = 4.85TKK70 pKa = 9.5WSPNIEE76 pKa = 4.44IIQKK80 pKa = 9.04IAEE83 pKa = 4.27YY84 pKa = 9.51YY85 pKa = 10.14QVDD88 pKa = 4.42FVQDD92 pKa = 3.71YY93 pKa = 11.26EE94 pKa = 4.39EE95 pKa = 4.59MGNLVYY101 pKa = 10.79GRR103 pKa = 11.84ATYY106 pKa = 10.54RR107 pKa = 11.84DD108 pKa = 4.52GILSDD113 pKa = 3.93IFLGDD118 pKa = 4.22DD119 pKa = 3.42DD120 pKa = 5.06FEE122 pKa = 6.92AYY124 pKa = 9.76EE125 pKa = 4.06QDD127 pKa = 3.62EE128 pKa = 4.59EE129 pKa = 4.17TDD131 pKa = 3.58LYY133 pKa = 11.08HH134 pKa = 7.46FEE136 pKa = 4.21GEE138 pKa = 4.47EE139 pKa = 4.0YY140 pKa = 10.59EE141 pKa = 4.14SDD143 pKa = 4.07YY144 pKa = 11.49EE145 pKa = 4.11ILEE148 pKa = 4.24TLLEE152 pKa = 4.18RR153 pKa = 11.84KK154 pKa = 9.21IVTLL158 pKa = 3.91

Molecular weight: 18.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7K6M2|A0A1X7K6M2_9SPHI Choline dehydrogenase OS=Sphingobacterium psychroaquaticum OX=561061 GN=SAMN05660862_2573 PE=4 SV=1
MM1 pKa = 7.92DD2 pKa = 5.27ANRR5 pKa = 11.84TEE7 pKa = 4.07LHH9 pKa = 5.42ATEE12 pKa = 4.53TLLQRR17 pKa = 11.84GVRR20 pKa = 11.84VKK22 pKa = 10.8ARR24 pKa = 11.84APLWLRR30 pKa = 11.84LLGKK34 pKa = 8.78KK35 pKa = 8.72TITLTLRR42 pKa = 11.84APTGGAFLRR51 pKa = 11.84MGEE54 pKa = 4.04WFLRR58 pKa = 11.84CQLSVDD64 pKa = 3.56QLQEE68 pKa = 3.61ISVQDD73 pKa = 3.47ALLFQVRR80 pKa = 11.84YY81 pKa = 9.42SRR83 pKa = 11.84CIYY86 pKa = 10.12RR87 pKa = 11.84ALACLFLVDD96 pKa = 5.2RR97 pKa = 11.84RR98 pKa = 11.84LTKK101 pKa = 10.81LFLRR105 pKa = 11.84PYY107 pKa = 10.72ANYY110 pKa = 9.71LRR112 pKa = 11.84EE113 pKa = 4.56AITPKK118 pKa = 10.36EE119 pKa = 3.78ALALLQLSILQGGSEE134 pKa = 4.81DD135 pKa = 3.72FMIITRR141 pKa = 11.84FLRR144 pKa = 11.84AKK146 pKa = 9.97MITAPKK152 pKa = 10.4KK153 pKa = 9.23MGHH156 pKa = 5.91

Molecular weight: 17.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3765

0

3765

1360707

23

2796

361.4

40.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.417 ± 0.039

0.701 ± 0.011

5.455 ± 0.025

5.953 ± 0.042

4.882 ± 0.031

6.921 ± 0.043

1.861 ± 0.019

6.922 ± 0.037

6.702 ± 0.037

9.412 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.016

5.414 ± 0.044

3.641 ± 0.023

3.89 ± 0.024

4.247 ± 0.029

6.278 ± 0.029

5.856 ± 0.037

6.718 ± 0.035

1.183 ± 0.016

4.217 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski