Amino acid dipepetide frequency for Polaromonas sp. OV174

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.652AlaAla: 16.652 ± 0.151
1.322AlaCys: 1.322 ± 0.029
6.091AlaAsp: 6.091 ± 0.07
6.273AlaGlu: 6.273 ± 0.085
3.944AlaPhe: 3.944 ± 0.056
10.269AlaGly: 10.269 ± 0.105
2.537AlaHis: 2.537 ± 0.044
5.39AlaIle: 5.39 ± 0.068
4.621AlaLys: 4.621 ± 0.065
14.878AlaLeu: 14.878 ± 0.138
3.7AlaMet: 3.7 ± 0.056
3.025AlaAsn: 3.025 ± 0.052
5.677AlaPro: 5.677 ± 0.073
6.238AlaGln: 6.238 ± 0.079
7.376AlaArg: 7.376 ± 0.088
7.442AlaSer: 7.442 ± 0.088
5.896AlaThr: 5.896 ± 0.064
8.748AlaVal: 8.748 ± 0.09
1.81AlaTrp: 1.81 ± 0.046
2.563AlaTyr: 2.563 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
1.176CysAla: 1.176 ± 0.03
0.131CysCys: 0.131 ± 0.01
0.525CysAsp: 0.525 ± 0.021
0.546CysGlu: 0.546 ± 0.019
0.35CysPhe: 0.35 ± 0.016
1.018CysGly: 1.018 ± 0.028
0.285CysHis: 0.285 ± 0.018
0.439CysIle: 0.439 ± 0.018
0.3CysLys: 0.3 ± 0.017
0.916CysLeu: 0.916 ± 0.029
0.231CysMet: 0.231 ± 0.012
0.245CysAsn: 0.245 ± 0.013
0.509CysPro: 0.509 ± 0.021
0.311CysGln: 0.311 ± 0.016
0.541CysArg: 0.541 ± 0.02
0.563CysSer: 0.563 ± 0.021
0.491CysThr: 0.491 ± 0.022
0.779CysVal: 0.779 ± 0.027
0.133CysTrp: 0.133 ± 0.01
0.213CysTyr: 0.213 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.289AspAla: 6.289 ± 0.076
0.485AspCys: 0.485 ± 0.019
2.304AspAsp: 2.304 ± 0.052
2.98AspGlu: 2.98 ± 0.053
2.134AspPhe: 2.134 ± 0.041
4.068AspGly: 4.068 ± 0.059
1.011AspHis: 1.011 ± 0.029
2.542AspIle: 2.542 ± 0.049
1.926AspLys: 1.926 ± 0.042
5.061AspLeu: 5.061 ± 0.06
1.306AspMet: 1.306 ± 0.032
1.234AspAsn: 1.234 ± 0.033
2.473AspPro: 2.473 ± 0.043
1.671AspGln: 1.671 ± 0.033
2.679AspArg: 2.679 ± 0.05
2.476AspSer: 2.476 ± 0.05
2.349AspThr: 2.349 ± 0.043
3.738AspVal: 3.738 ± 0.064
0.884AspTrp: 0.884 ± 0.026
1.475AspTyr: 1.475 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
6.508GluAla: 6.508 ± 0.089
0.39GluCys: 0.39 ± 0.02
2.099GluAsp: 2.099 ± 0.036
2.462GluGlu: 2.462 ± 0.049
1.907GluPhe: 1.907 ± 0.039
3.407GluGly: 3.407 ± 0.052
1.292GluHis: 1.292 ± 0.038
2.818GluIle: 2.818 ± 0.052
2.355GluLys: 2.355 ± 0.046
6.03GluLeu: 6.03 ± 0.069
1.364GluMet: 1.364 ± 0.031
1.417GluAsn: 1.417 ± 0.032
2.263GluPro: 2.263 ± 0.047
2.675GluGln: 2.675 ± 0.052
4.062GluArg: 4.062 ± 0.057
2.664GluSer: 2.664 ± 0.048
2.422GluThr: 2.422 ± 0.041
3.948GluVal: 3.948 ± 0.06
0.671GluTrp: 0.671 ± 0.022
0.995GluTyr: 0.995 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.069PheAla: 4.069 ± 0.056
0.407PheCys: 0.407 ± 0.017
2.381PheAsp: 2.381 ± 0.041
2.114PheGlu: 2.114 ± 0.039
1.49PhePhe: 1.49 ± 0.039
3.405PheGly: 3.405 ± 0.056
0.7PheHis: 0.7 ± 0.024
1.631PheIle: 1.631 ± 0.036
1.493PheLys: 1.493 ± 0.037
3.081PheLeu: 3.081 ± 0.052
0.903PheMet: 0.903 ± 0.03
1.204PheAsn: 1.204 ± 0.029
1.589PhePro: 1.589 ± 0.033
1.119PheGln: 1.119 ± 0.029
1.784PheArg: 1.784 ± 0.038
2.47PheSer: 2.47 ± 0.043
1.883PheThr: 1.883 ± 0.039
2.731PheVal: 2.731 ± 0.05
0.543PheTrp: 0.543 ± 0.022
0.876PheTyr: 0.876 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
8.6GlyAla: 8.6 ± 0.077
0.93GlyCys: 0.93 ± 0.027
3.536GlyAsp: 3.536 ± 0.059
4.183GlyGlu: 4.183 ± 0.065
3.25GlyPhe: 3.25 ± 0.052
6.576GlyGly: 6.576 ± 0.1
1.831GlyHis: 1.831 ± 0.038
4.101GlyIle: 4.101 ± 0.059
3.7GlyLys: 3.7 ± 0.065
9.243GlyLeu: 9.243 ± 0.093
2.355GlyMet: 2.355 ± 0.043
2.227GlyAsn: 2.227 ± 0.041
2.873GlyPro: 2.873 ± 0.052
3.656GlyGln: 3.656 ± 0.056
4.802GlyArg: 4.802 ± 0.067
4.83GlySer: 4.83 ± 0.065
4.093GlyThr: 4.093 ± 0.059
6.415GlyVal: 6.415 ± 0.072
1.345GlyTrp: 1.345 ± 0.032
2.261GlyTyr: 2.261 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.63HisAla: 2.63 ± 0.045
0.3HisCys: 0.3 ± 0.015
1.111HisAsp: 1.111 ± 0.031
1.107HisGlu: 1.107 ± 0.029
0.873HisPhe: 0.873 ± 0.026
1.999HisGly: 1.999 ± 0.039
0.591HisHis: 0.591 ± 0.026
1.031HisIle: 1.031 ± 0.027
0.676HisLys: 0.676 ± 0.022
2.172HisLeu: 2.172 ± 0.041
0.534HisMet: 0.534 ± 0.022
0.541HisAsn: 0.541 ± 0.021
1.414HisPro: 1.414 ± 0.034
0.851HisGln: 0.851 ± 0.022
1.256HisArg: 1.256 ± 0.034
1.16HisSer: 1.16 ± 0.03
0.988HisThr: 0.988 ± 0.029
1.546HisVal: 1.546 ± 0.036
0.37HisTrp: 0.37 ± 0.017
0.642HisTyr: 0.642 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
6.212IleAla: 6.212 ± 0.071
0.504IleCys: 0.504 ± 0.022
2.964IleAsp: 2.964 ± 0.053
3.15IleGlu: 3.15 ± 0.053
1.498IlePhe: 1.498 ± 0.037
4.062IleGly: 4.062 ± 0.061
0.89IleHis: 0.89 ± 0.026
1.798IleIle: 1.798 ± 0.043
2.013IleLys: 2.013 ± 0.05
3.621IleLeu: 3.621 ± 0.055
0.918IleMet: 0.918 ± 0.024
1.678IleAsn: 1.678 ± 0.036
2.219IlePro: 2.219 ± 0.042
1.475IleGln: 1.475 ± 0.036
2.588IleArg: 2.588 ± 0.042
2.922IleSer: 2.922 ± 0.052
2.629IleThr: 2.629 ± 0.049
3.552IleVal: 3.552 ± 0.06
0.492IleTrp: 0.492 ± 0.021
1.114IleTyr: 1.114 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
4.746LysAla: 4.746 ± 0.073
0.208LysCys: 0.208 ± 0.013
1.886LysAsp: 1.886 ± 0.042
1.814LysGlu: 1.814 ± 0.045
1.174LysPhe: 1.174 ± 0.035
2.765LysGly: 2.765 ± 0.053
0.783LysHis: 0.783 ± 0.023
1.893LysIle: 1.893 ± 0.046
1.86LysLys: 1.86 ± 0.051
4.355LysLeu: 4.355 ± 0.061
1.003LysMet: 1.003 ± 0.026
1.305LysAsn: 1.305 ± 0.035
2.432LysPro: 2.432 ± 0.047
1.454LysGln: 1.454 ± 0.035
2.467LysArg: 2.467 ± 0.05
2.268LysSer: 2.268 ± 0.043
2.408LysThr: 2.408 ± 0.05
2.847LysVal: 2.847 ± 0.054
0.412LysTrp: 0.412 ± 0.018
0.782LysTyr: 0.782 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
14.751LeuAla: 14.751 ± 0.142
1.093LeuCys: 1.093 ± 0.032
5.487LeuAsp: 5.487 ± 0.069
5.297LeuGlu: 5.297 ± 0.07
3.547LeuPhe: 3.547 ± 0.06
8.885LeuGly: 8.885 ± 0.097
2.23LeuHis: 2.23 ± 0.044
4.845LeuIle: 4.845 ± 0.071
4.418LeuLys: 4.418 ± 0.064
12.206LeuLeu: 12.206 ± 0.146
2.873LeuMet: 2.873 ± 0.046
3.255LeuAsn: 3.255 ± 0.052
6.051LeuPro: 6.051 ± 0.074
4.923LeuGln: 4.923 ± 0.072
7.008LeuArg: 7.008 ± 0.097
6.912LeuSer: 6.912 ± 0.09
5.576LeuThr: 5.576 ± 0.073
7.79LeuVal: 7.79 ± 0.097
1.34LeuTrp: 1.34 ± 0.034
2.056LeuTyr: 2.056 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
3.353MetAla: 3.353 ± 0.05
0.185MetCys: 0.185 ± 0.012
1.19MetAsp: 1.19 ± 0.027
1.124MetGlu: 1.124 ± 0.027
0.742MetPhe: 0.742 ± 0.025
2.047MetGly: 2.047 ± 0.04
0.551MetHis: 0.551 ± 0.019
1.077MetIle: 1.077 ± 0.031
1.252MetLys: 1.252 ± 0.03
2.964MetLeu: 2.964 ± 0.058
0.667MetMet: 0.667 ± 0.022
0.961MetAsn: 0.961 ± 0.027
1.458MetPro: 1.458 ± 0.033
1.161MetGln: 1.161 ± 0.028
1.646MetArg: 1.646 ± 0.035
1.793MetSer: 1.793 ± 0.034
1.686MetThr: 1.686 ± 0.038
1.89MetVal: 1.89 ± 0.034
0.211MetTrp: 0.211 ± 0.011
0.436MetTyr: 0.436 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.465AsnAla: 3.465 ± 0.053
0.294AsnCys: 0.294 ± 0.015
1.316AsnAsp: 1.316 ± 0.03
1.356AsnGlu: 1.356 ± 0.034
1.056AsnPhe: 1.056 ± 0.024
2.249AsnGly: 2.249 ± 0.046
0.572AsnHis: 0.572 ± 0.023
1.398AsnIle: 1.398 ± 0.036
1.035AsnLys: 1.035 ± 0.026
2.861AsnLeu: 2.861 ± 0.043
0.664AsnMet: 0.664 ± 0.023
0.803AsnAsn: 0.803 ± 0.026
1.952AsnPro: 1.952 ± 0.038
1.075AsnGln: 1.075 ± 0.029
1.608AsnArg: 1.608 ± 0.036
1.561AsnSer: 1.561 ± 0.039
1.59AsnThr: 1.59 ± 0.033
1.929AsnVal: 1.929 ± 0.04
0.45AsnTrp: 0.45 ± 0.017
0.792AsnTyr: 0.792 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
6.725ProAla: 6.725 ± 0.079
0.373ProCys: 0.373 ± 0.018
2.867ProAsp: 2.867 ± 0.048
3.23ProGlu: 3.23 ± 0.052
1.808ProPhe: 1.808 ± 0.035
4.296ProGly: 4.296 ± 0.054
1.118ProHis: 1.118 ± 0.025
1.965ProIle: 1.965 ± 0.033
1.821ProLys: 1.821 ± 0.034
5.323ProLeu: 5.323 ± 0.068
1.333ProMet: 1.333 ± 0.035
1.351ProAsn: 1.351 ± 0.03
2.305ProPro: 2.305 ± 0.047
2.224ProGln: 2.224 ± 0.041
2.502ProArg: 2.502 ± 0.051
2.908ProSer: 2.908 ± 0.049
2.416ProThr: 2.416 ± 0.046
4.111ProVal: 4.111 ± 0.06
0.761ProTrp: 0.761 ± 0.025
1.237ProTyr: 1.237 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
6.459GlnAla: 6.459 ± 0.086
0.334GlnCys: 0.334 ± 0.016
1.758GlnAsp: 1.758 ± 0.035
1.799GlnGlu: 1.799 ± 0.044
1.347GlnPhe: 1.347 ± 0.035
3.23GlnGly: 3.23 ± 0.047
1.038GlnHis: 1.038 ± 0.033
1.955GlnIle: 1.955 ± 0.036
1.363GlnLys: 1.363 ± 0.032
4.913GlnLeu: 4.913 ± 0.069
1.009GlnMet: 1.009 ± 0.024
0.994GlnAsn: 0.994 ± 0.026
2.337GlnPro: 2.337 ± 0.04
2.18GlnGln: 2.18 ± 0.061
3.294GlnArg: 3.294 ± 0.055
2.297GlnSer: 2.297 ± 0.043
2.214GlnThr: 2.214 ± 0.041
3.147GlnVal: 3.147 ± 0.053
0.647GlnTrp: 0.647 ± 0.021
0.78GlnTyr: 0.78 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
6.691ArgAla: 6.691 ± 0.075
0.595ArgCys: 0.595 ± 0.022
2.991ArgAsp: 2.991 ± 0.051
3.683ArgGlu: 3.683 ± 0.059
2.476ArgPhe: 2.476 ± 0.045
4.062ArgGly: 4.062 ± 0.062
1.575ArgHis: 1.575 ± 0.036
3.28ArgIle: 3.28 ± 0.05
2.206ArgLys: 2.206 ± 0.043
7.141ArgLeu: 7.141 ± 0.086
1.714ArgMet: 1.714 ± 0.041
1.705ArgAsn: 1.705 ± 0.035
2.852ArgPro: 2.852 ± 0.053
3.055ArgGln: 3.055 ± 0.055
3.993ArgArg: 3.993 ± 0.061
3.416ArgSer: 3.416 ± 0.055
2.71ArgThr: 2.71 ± 0.047
4.434ArgVal: 4.434 ± 0.066
1.029ArgTrp: 1.029 ± 0.029
1.678ArgTyr: 1.678 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.99SerAla: 6.99 ± 0.08
0.548SerCys: 0.548 ± 0.021
2.708SerAsp: 2.708 ± 0.051
2.797SerGlu: 2.797 ± 0.046
2.373SerPhe: 2.373 ± 0.043
5.491SerGly: 5.491 ± 0.069
1.328SerHis: 1.328 ± 0.034
2.759SerIle: 2.759 ± 0.048
2.048SerLys: 2.048 ± 0.045
6.358SerLeu: 6.358 ± 0.078
1.608SerMet: 1.608 ± 0.036
1.603SerAsn: 1.603 ± 0.033
3.132SerPro: 3.132 ± 0.05
2.297SerGln: 2.297 ± 0.042
3.675SerArg: 3.675 ± 0.05
3.747SerSer: 3.747 ± 0.061
3.015SerThr: 3.015 ± 0.049
4.179SerVal: 4.179 ± 0.054
0.844SerTrp: 0.844 ± 0.027
1.466SerTyr: 1.466 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
5.975ThrAla: 5.975 ± 0.072
0.401ThrCys: 0.401 ± 0.019
2.378ThrAsp: 2.378 ± 0.038
2.375ThrGlu: 2.375 ± 0.041
1.717ThrPhe: 1.717 ± 0.036
4.476ThrGly: 4.476 ± 0.063
1.188ThrHis: 1.188 ± 0.029
2.187ThrIle: 2.187 ± 0.039
1.452ThrLys: 1.452 ± 0.039
6.505ThrLeu: 6.505 ± 0.068
1.174ThrMet: 1.174 ± 0.034
1.194ThrAsn: 1.194 ± 0.032
3.457ThrPro: 3.457 ± 0.052
2.123ThrGln: 2.123 ± 0.039
2.972ThrArg: 2.972 ± 0.05
2.906ThrSer: 2.906 ± 0.053
2.653ThrThr: 2.653 ± 0.042
4.048ThrVal: 4.048 ± 0.062
0.612ThrTrp: 0.612 ± 0.023
1.043ThrTyr: 1.043 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
9.235ValAla: 9.235 ± 0.086
0.763ValCys: 0.763 ± 0.022
3.721ValAsp: 3.721 ± 0.054
3.772ValGlu: 3.772 ± 0.051
2.729ValPhe: 2.729 ± 0.053
5.497ValGly: 5.497 ± 0.072
1.469ValHis: 1.469 ± 0.039
3.674ValIle: 3.674 ± 0.057
2.946ValLys: 2.946 ± 0.053
8.223ValLeu: 8.223 ± 0.09
2.137ValMet: 2.137 ± 0.036
2.276ValAsn: 2.276 ± 0.039
3.746ValPro: 3.746 ± 0.058
2.904ValGln: 2.904 ± 0.046
4.413ValArg: 4.413 ± 0.054
4.453ValSer: 4.453 ± 0.069
3.972ValThr: 3.972 ± 0.054
6.161ValVal: 6.161 ± 0.081
0.931ValTrp: 0.931 ± 0.027
1.574ValTyr: 1.574 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.347TrpAla: 1.347 ± 0.029
0.158TrpCys: 0.158 ± 0.01
0.596TrpAsp: 0.596 ± 0.023
0.508TrpGlu: 0.508 ± 0.019
0.523TrpPhe: 0.523 ± 0.023
0.948TrpGly: 0.948 ± 0.026
0.36TrpHis: 0.36 ± 0.017
0.642TrpIle: 0.642 ± 0.022
0.517TrpLys: 0.517 ± 0.02
2.185TrpLeu: 2.185 ± 0.046
0.414TrpMet: 0.414 ± 0.017
0.404TrpAsn: 0.404 ± 0.017
0.719TrpPro: 0.719 ± 0.027
0.744TrpGln: 0.744 ± 0.024
1.065TrpArg: 1.065 ± 0.03
0.818TrpSer: 0.818 ± 0.025
0.663TrpThr: 0.663 ± 0.024
0.977TrpVal: 0.977 ± 0.025
0.268TrpTrp: 0.268 ± 0.016
0.276TrpTyr: 0.276 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.578TyrAla: 2.578 ± 0.043
0.282TyrCys: 0.282 ± 0.014
1.173TyrAsp: 1.173 ± 0.027
1.18TyrGlu: 1.18 ± 0.029
0.934TyrPhe: 0.934 ± 0.027
2.059TyrGly: 2.059 ± 0.043
0.442TyrHis: 0.442 ± 0.018
0.876TyrIle: 0.876 ± 0.022
0.861TyrLys: 0.861 ± 0.028
2.511TyrLeu: 2.511 ± 0.042
0.485TyrMet: 0.485 ± 0.022
0.603TyrAsn: 0.603 ± 0.024
1.219TyrPro: 1.219 ± 0.03
0.957TyrGln: 0.957 ± 0.03
1.552TyrArg: 1.552 ± 0.035
1.358TyrSer: 1.358 ± 0.031
1.179TyrThr: 1.179 ± 0.036
1.628TyrVal: 1.628 ± 0.036
0.382TyrTrp: 0.382 ± 0.018
0.571TyrTyr: 0.571 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4300 proteins (1345509 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski