Lates calcarifer (Barramundi) (Holocentrus calcarifer)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57547 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4W6FBF1|A0A4W6FBF1_LATCA Mitochondrial ribosomal protein L38 OS=Lates calcarifer OX=8187 GN=mrpl38 PE=4 SV=1
MM1 pKa = 7.71LSTPSILDD9 pKa = 3.72HH10 pKa = 7.06PPSPMDD16 pKa = 3.37TSDD19 pKa = 4.61LGFSPHH25 pKa = 5.39STALDD30 pKa = 3.93FGDD33 pKa = 3.89PTLDD37 pKa = 3.58SMDD40 pKa = 3.5WLDD43 pKa = 3.34ISMVGIGGTSLAPLAPHH60 pKa = 6.82TPPSVFSADD69 pKa = 3.8FLDD72 pKa = 4.3STDD75 pKa = 4.75LQLHH79 pKa = 6.06WEE81 pKa = 4.25SCLL84 pKa = 3.68

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4W6E993|A0A4W6E993_LATCA Uncharacterized protein OS=Lates calcarifer OX=8187 PE=3 SV=1
SS1 pKa = 4.63SHH3 pKa = 5.15KK4 pKa = 8.9TFRR7 pKa = 11.84IKK9 pKa = 10.64RR10 pKa = 11.84FLAKK14 pKa = 9.71KK15 pKa = 9.58QKK17 pKa = 8.69QNRR20 pKa = 11.84PIPQWIRR27 pKa = 11.84MKK29 pKa = 9.89TGNKK33 pKa = 8.61IRR35 pKa = 11.84YY36 pKa = 7.09NSKK39 pKa = 8.3RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 3.95WRR44 pKa = 11.84RR45 pKa = 11.84TKK47 pKa = 10.83LGLL50 pKa = 3.67

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24715

32832

57547

37319313

17

6353

648.5

72.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.441 ± 0.008

2.283 ± 0.008

5.242 ± 0.006

6.787 ± 0.011

3.815 ± 0.007

6.254 ± 0.011

2.631 ± 0.004

4.705 ± 0.007

5.635 ± 0.01

9.717 ± 0.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.005

3.929 ± 0.006

5.395 ± 0.01

4.664 ± 0.008

5.536 ± 0.007

8.248 ± 0.011

5.667 ± 0.008

6.501 ± 0.007

1.193 ± 0.003

2.926 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski