Pseudomonas phage Stalingrad

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Troedvirus; unclassified Troedvirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LJ68|A0A7G8LJ68_9CAUD RNA polymerase inhibitor OS=Pseudomonas phage Stalingrad OX=2762287 PE=4 SV=1
MM1 pKa = 7.55SPQEE5 pKa = 5.62LLDD8 pKa = 3.63LWASSGSDD16 pKa = 3.38DD17 pKa = 3.79GSSRR21 pKa = 11.84HH22 pKa = 6.93DD23 pKa = 3.23ILNAEE28 pKa = 4.84DD29 pKa = 5.74YY30 pKa = 11.04FDD32 pKa = 3.5GWEE35 pKa = 4.11VADD38 pKa = 4.91ADD40 pKa = 5.52DD41 pKa = 3.41WTQDD45 pKa = 2.87HH46 pKa = 7.19KK47 pKa = 10.89YY48 pKa = 9.21QHH50 pKa = 5.93IAKK53 pKa = 9.34VIKK56 pKa = 8.46HH57 pKa = 5.41TEE59 pKa = 3.78SGRR62 pKa = 11.84CFCVNASRR70 pKa = 11.84SGSYY74 pKa = 8.05HH75 pKa = 5.62TDD77 pKa = 2.13WYY79 pKa = 11.26YY80 pKa = 11.55SYY82 pKa = 11.82DD83 pKa = 3.55DD84 pKa = 3.74LTEE87 pKa = 4.05VKK89 pKa = 10.4AVEE92 pKa = 4.1EE93 pKa = 4.48VIKK96 pKa = 8.67VTKK99 pKa = 9.32WVTVV103 pKa = 3.46

Molecular weight: 11.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LJ60|A0A7G8LJ60_9CAUD DNA polymerase OS=Pseudomonas phage Stalingrad OX=2762287 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.36ALRR5 pKa = 11.84NFDD8 pKa = 3.28IVTALVVLAGRR19 pKa = 11.84IQAKK23 pKa = 9.95RR24 pKa = 11.84IEE26 pKa = 4.13RR27 pKa = 11.84LKK29 pKa = 10.81ARR31 pKa = 11.84EE32 pKa = 3.74VALRR36 pKa = 11.84LAIDD40 pKa = 3.9SACAALTTTQRR51 pKa = 11.84DD52 pKa = 3.37RR53 pKa = 11.84LKK55 pKa = 11.05AEE57 pKa = 3.54QRR59 pKa = 11.84KK60 pKa = 9.73IGEE63 pKa = 4.07

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12493

51

1326

260.3

28.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.693 ± 0.562

0.929 ± 0.159

6.444 ± 0.224

6.876 ± 0.333

3.602 ± 0.149

8.045 ± 0.305

2.217 ± 0.238

4.827 ± 0.179

5.811 ± 0.374

7.948 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.834 ± 0.185

3.794 ± 0.275

3.866 ± 0.177

4.37 ± 0.366

5.947 ± 0.198

5.595 ± 0.278

5.667 ± 0.272

7.004 ± 0.305

1.617 ± 0.166

2.914 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski