Shigella phage vB_SflS-ISF001

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tunavirinae; Tunavirus; Shigella virus ISF001

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GQ34|A0A2D1GQ34_9CAUD Uncharacterized protein OS=Shigella phage vB_SflS-ISF001 OX=2048005 GN=FLXISF001_078 PE=4 SV=1
MM1 pKa = 6.97MVSVDD6 pKa = 5.94KK7 pKa = 11.15FFTCNKK13 pKa = 8.44SSEE16 pKa = 4.27VFEE19 pKa = 5.21RR20 pKa = 11.84VHH22 pKa = 6.29TDD24 pKa = 2.79NADD27 pKa = 3.48FMHH30 pKa = 7.23DD31 pKa = 3.33GCDD34 pKa = 3.28VFIEE38 pKa = 4.6VKK40 pKa = 10.8DD41 pKa = 3.54SDD43 pKa = 4.02YY44 pKa = 11.76DD45 pKa = 3.41DD46 pKa = 3.04WVYY49 pKa = 11.79YY50 pKa = 10.51NLAVYY55 pKa = 7.54TQSFTPIADD64 pKa = 3.83EE65 pKa = 4.73AEE67 pKa = 4.26EE68 pKa = 4.03AA69 pKa = 3.95

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GQ79|A0A2D1GQ79_9CAUD Putative tail fiber OS=Shigella phage vB_SflS-ISF001 OX=2048005 GN=FLXISF001_015 PE=4 SV=1
MM1 pKa = 7.58NIQRR5 pKa = 11.84IAEE8 pKa = 4.24STGEE12 pKa = 3.42IDD14 pKa = 3.58TRR16 pKa = 11.84HH17 pKa = 5.71NNGNNGTRR25 pKa = 11.84RR26 pKa = 11.84GKK28 pKa = 10.57DD29 pKa = 2.74KK30 pKa = 10.57MPRR33 pKa = 11.84LRR35 pKa = 11.84CGFYY39 pKa = 9.96IHH41 pKa = 7.79IEE43 pKa = 4.0EE44 pKa = 4.4TRR46 pKa = 11.84AGLPARR52 pKa = 11.84LDD54 pKa = 3.62ALIVYY59 pKa = 9.35YY60 pKa = 10.58GGPAASAKK68 pKa = 9.68ALKK71 pKa = 10.67VSIQTVQGWKK81 pKa = 8.42VRR83 pKa = 11.84NMISWQAAEE92 pKa = 3.96AAHH95 pKa = 6.07RR96 pKa = 11.84ASRR99 pKa = 11.84RR100 pKa = 11.84QGCKK104 pKa = 9.54GFRR107 pKa = 11.84AAWLRR112 pKa = 11.84FALKK116 pKa = 10.2FDD118 pKa = 4.3GNGKK122 pKa = 9.4CLEE125 pKa = 4.21KK126 pKa = 10.4RR127 pKa = 11.84CKK129 pKa = 9.3IKK131 pKa = 10.91KK132 pKa = 9.41FMRR135 pKa = 11.84VVKK138 pKa = 10.61KK139 pKa = 10.74EE140 pKa = 4.29DD141 pKa = 2.97IGTTNSIFSS150 pKa = 3.71

Molecular weight: 16.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

14784

49

1127

189.5

21.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.934 ± 0.406

1.393 ± 0.162

6.128 ± 0.255

6.541 ± 0.241

3.862 ± 0.194

7.143 ± 0.241

1.772 ± 0.215

7.048 ± 0.19

6.893 ± 0.3

6.548 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.098 ± 0.163

4.884 ± 0.282

3.592 ± 0.237

3.727 ± 0.249

4.843 ± 0.196

6.683 ± 0.248

5.587 ± 0.196

7.325 ± 0.286

1.454 ± 0.096

3.544 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski