Amino acid dipepetide frequency for Acinetobacter phage Loki

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.034AlaAla: 11.034 ± 2.011
0.605AlaCys: 0.605 ± 0.234
3.854AlaAsp: 3.854 ± 0.571
4.912AlaGlu: 4.912 ± 0.587
3.628AlaPhe: 3.628 ± 0.408
5.441AlaGly: 5.441 ± 0.766
1.738AlaHis: 1.738 ± 0.305
6.575AlaIle: 6.575 ± 0.778
5.593AlaLys: 5.593 ± 0.851
7.784AlaLeu: 7.784 ± 1.403
2.192AlaMet: 2.192 ± 0.465
6.651AlaAsn: 6.651 ± 0.727
3.023AlaPro: 3.023 ± 0.394
4.383AlaGln: 4.383 ± 0.664
3.552AlaArg: 3.552 ± 0.582
6.122AlaSer: 6.122 ± 0.764
6.273AlaThr: 6.273 ± 1.025
6.424AlaVal: 6.424 ± 0.923
1.436AlaTrp: 1.436 ± 0.337
3.25AlaTyr: 3.25 ± 0.463
0.0AlaXaa: 0.0 ± 0.0
Cys
0.831CysAla: 0.831 ± 0.269
0.529CysCys: 0.529 ± 0.184
0.302CysAsp: 0.302 ± 0.17
0.605CysGlu: 0.605 ± 0.194
0.302CysPhe: 0.302 ± 0.147
0.68CysGly: 0.68 ± 0.376
0.151CysHis: 0.151 ± 0.106
0.378CysIle: 0.378 ± 0.163
0.68CysLys: 0.68 ± 0.267
0.907CysLeu: 0.907 ± 0.297
0.151CysMet: 0.151 ± 0.115
0.453CysAsn: 0.453 ± 0.258
0.831CysPro: 0.831 ± 0.306
0.605CysGln: 0.605 ± 0.198
0.453CysArg: 0.453 ± 0.227
0.302CysSer: 0.302 ± 0.148
0.151CysThr: 0.151 ± 0.106
0.68CysVal: 0.68 ± 0.269
0.151CysTrp: 0.151 ± 0.12
0.378CysTyr: 0.378 ± 0.207
0.0CysXaa: 0.0 ± 0.0
Asp
5.441AspAla: 5.441 ± 0.633
0.529AspCys: 0.529 ± 0.205
3.476AspAsp: 3.476 ± 0.635
4.308AspGlu: 4.308 ± 0.679
2.041AspPhe: 2.041 ± 0.378
5.895AspGly: 5.895 ± 0.729
0.68AspHis: 0.68 ± 0.179
3.854AspIle: 3.854 ± 0.572
3.325AspLys: 3.325 ± 0.458
5.366AspLeu: 5.366 ± 0.823
1.587AspMet: 1.587 ± 0.353
2.267AspAsn: 2.267 ± 0.551
2.041AspPro: 2.041 ± 0.392
1.511AspGln: 1.511 ± 0.34
2.947AspArg: 2.947 ± 0.488
3.779AspSer: 3.779 ± 0.519
2.947AspThr: 2.947 ± 0.442
3.779AspVal: 3.779 ± 0.455
0.756AspTrp: 0.756 ± 0.28
2.721AspTyr: 2.721 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
4.686GluAla: 4.686 ± 0.528
0.378GluCys: 0.378 ± 0.144
2.418GluAsp: 2.418 ± 0.513
3.25GluGlu: 3.25 ± 0.53
3.174GluPhe: 3.174 ± 0.473
3.25GluGly: 3.25 ± 0.415
0.68GluHis: 0.68 ± 0.326
5.215GluIle: 5.215 ± 0.676
3.779GluLys: 3.779 ± 0.58
5.744GluLeu: 5.744 ± 0.558
1.738GluMet: 1.738 ± 0.365
3.023GluAsn: 3.023 ± 0.536
2.645GluPro: 2.645 ± 0.482
3.628GluGln: 3.628 ± 0.458
2.267GluArg: 2.267 ± 0.539
2.947GluSer: 2.947 ± 0.524
3.476GluThr: 3.476 ± 0.743
3.628GluVal: 3.628 ± 0.533
1.436GluTrp: 1.436 ± 0.412
2.721GluTyr: 2.721 ± 0.556
0.0GluXaa: 0.0 ± 0.0
Phe
3.099PheAla: 3.099 ± 0.607
0.453PheCys: 0.453 ± 0.239
4.005PheAsp: 4.005 ± 0.637
2.57PheGlu: 2.57 ± 0.403
1.209PhePhe: 1.209 ± 0.33
3.25PheGly: 3.25 ± 0.459
0.227PheHis: 0.227 ± 0.117
2.872PheIle: 2.872 ± 0.606
2.721PheLys: 2.721 ± 0.489
1.889PheLeu: 1.889 ± 0.298
1.058PheMet: 1.058 ± 0.191
2.947PheAsn: 2.947 ± 0.595
1.436PhePro: 1.436 ± 0.343
1.058PheGln: 1.058 ± 0.279
1.738PheArg: 1.738 ± 0.377
1.587PheSer: 1.587 ± 0.32
2.721PheThr: 2.721 ± 0.391
3.023PheVal: 3.023 ± 0.361
0.756PheTrp: 0.756 ± 0.221
1.285PheTyr: 1.285 ± 0.305
0.0PheXaa: 0.0 ± 0.0
Gly
5.819GlyAla: 5.819 ± 0.666
0.378GlyCys: 0.378 ± 0.276
4.232GlyAsp: 4.232 ± 0.597
4.61GlyGlu: 4.61 ± 0.572
4.081GlyPhe: 4.081 ± 0.53
6.424GlyGly: 6.424 ± 1.067
1.209GlyHis: 1.209 ± 0.349
3.854GlyIle: 3.854 ± 0.466
3.628GlyLys: 3.628 ± 0.474
5.744GlyLeu: 5.744 ± 0.609
1.436GlyMet: 1.436 ± 0.319
3.325GlyAsn: 3.325 ± 0.858
0.0GlyPro: 0.0 ± 0.0
2.721GlyGln: 2.721 ± 0.535
2.116GlyArg: 2.116 ± 0.329
4.383GlySer: 4.383 ± 0.626
4.157GlyThr: 4.157 ± 0.668
6.651GlyVal: 6.651 ± 0.817
1.814GlyTrp: 1.814 ± 0.428
2.721GlyTyr: 2.721 ± 0.441
0.0GlyXaa: 0.0 ± 0.0
His
0.982HisAla: 0.982 ± 0.337
0.151HisCys: 0.151 ± 0.143
0.453HisAsp: 0.453 ± 0.179
1.134HisGlu: 1.134 ± 0.293
0.529HisPhe: 0.529 ± 0.198
0.529HisGly: 0.529 ± 0.164
0.378HisHis: 0.378 ± 0.167
1.587HisIle: 1.587 ± 0.308
0.756HisLys: 0.756 ± 0.273
1.058HisLeu: 1.058 ± 0.316
0.151HisMet: 0.151 ± 0.099
0.756HisAsn: 0.756 ± 0.259
0.529HisPro: 0.529 ± 0.236
0.378HisGln: 0.378 ± 0.197
0.907HisArg: 0.907 ± 0.312
0.907HisSer: 0.907 ± 0.323
0.453HisThr: 0.453 ± 0.161
0.907HisVal: 0.907 ± 0.29
0.227HisTrp: 0.227 ± 0.13
0.831HisTyr: 0.831 ± 0.287
0.0HisXaa: 0.0 ± 0.0
Ile
6.197IleAla: 6.197 ± 0.6
0.831IleCys: 0.831 ± 0.287
4.534IleAsp: 4.534 ± 0.495
4.534IleGlu: 4.534 ± 0.498
2.192IlePhe: 2.192 ± 0.394
3.93IleGly: 3.93 ± 0.557
0.529IleHis: 0.529 ± 0.22
3.023IleIle: 3.023 ± 0.485
4.686IleLys: 4.686 ± 0.605
2.645IleLeu: 2.645 ± 0.493
1.36IleMet: 1.36 ± 0.385
3.703IleAsn: 3.703 ± 0.522
2.947IlePro: 2.947 ± 0.467
2.041IleGln: 2.041 ± 0.37
2.418IleArg: 2.418 ± 0.359
3.325IleSer: 3.325 ± 0.554
3.93IleThr: 3.93 ± 0.601
4.459IleVal: 4.459 ± 0.564
0.907IleTrp: 0.907 ± 0.217
2.796IleTyr: 2.796 ± 0.504
0.0IleXaa: 0.0 ± 0.0
Lys
6.575LysAla: 6.575 ± 0.801
0.605LysCys: 0.605 ± 0.306
4.837LysAsp: 4.837 ± 0.56
4.232LysGlu: 4.232 ± 0.636
1.738LysPhe: 1.738 ± 0.422
3.325LysGly: 3.325 ± 0.42
1.36LysHis: 1.36 ± 0.415
4.005LysIle: 4.005 ± 0.652
3.854LysLys: 3.854 ± 0.688
6.499LysLeu: 6.499 ± 0.703
1.965LysMet: 1.965 ± 0.349
2.116LysAsn: 2.116 ± 0.378
2.192LysPro: 2.192 ± 0.494
2.721LysGln: 2.721 ± 0.596
2.721LysArg: 2.721 ± 0.502
3.628LysSer: 3.628 ± 0.417
4.61LysThr: 4.61 ± 0.545
3.628LysVal: 3.628 ± 0.485
0.907LysTrp: 0.907 ± 0.264
1.965LysTyr: 1.965 ± 0.414
0.0LysXaa: 0.0 ± 0.0
Leu
7.104LeuAla: 7.104 ± 1.142
0.605LeuCys: 0.605 ± 0.227
4.837LeuAsp: 4.837 ± 0.552
5.517LeuGlu: 5.517 ± 0.735
3.779LeuPhe: 3.779 ± 0.596
4.534LeuGly: 4.534 ± 0.658
1.436LeuHis: 1.436 ± 0.449
4.081LeuIle: 4.081 ± 0.625
5.744LeuLys: 5.744 ± 0.674
5.366LeuLeu: 5.366 ± 0.588
1.814LeuMet: 1.814 ± 0.471
5.29LeuAsn: 5.29 ± 0.69
3.779LeuPro: 3.779 ± 0.467
3.703LeuGln: 3.703 ± 0.573
3.25LeuArg: 3.25 ± 0.406
5.215LeuSer: 5.215 ± 0.714
5.668LeuThr: 5.668 ± 0.685
3.779LeuVal: 3.779 ± 0.633
0.831LeuTrp: 0.831 ± 0.217
2.947LeuTyr: 2.947 ± 0.454
0.0LeuXaa: 0.0 ± 0.0
Met
2.721MetAla: 2.721 ± 0.479
0.0MetCys: 0.0 ± 0.0
1.587MetAsp: 1.587 ± 0.348
0.529MetGlu: 0.529 ± 0.235
0.605MetPhe: 0.605 ± 0.306
1.814MetGly: 1.814 ± 0.429
0.378MetHis: 0.378 ± 0.164
1.058MetIle: 1.058 ± 0.324
1.663MetLys: 1.663 ± 0.432
2.57MetLeu: 2.57 ± 0.392
0.529MetMet: 0.529 ± 0.196
1.058MetAsn: 1.058 ± 0.3
1.663MetPro: 1.663 ± 0.333
1.511MetGln: 1.511 ± 0.315
0.68MetArg: 0.68 ± 0.192
1.965MetSer: 1.965 ± 0.376
1.663MetThr: 1.663 ± 0.36
1.436MetVal: 1.436 ± 0.275
0.453MetTrp: 0.453 ± 0.165
0.756MetTyr: 0.756 ± 0.191
0.0MetXaa: 0.0 ± 0.0
Asn
5.819AsnAla: 5.819 ± 1.044
0.68AsnCys: 0.68 ± 0.22
3.476AsnAsp: 3.476 ± 0.435
4.005AsnGlu: 4.005 ± 0.678
1.889AsnPhe: 1.889 ± 0.39
5.668AsnGly: 5.668 ± 0.744
0.529AsnHis: 0.529 ± 0.206
2.343AsnIle: 2.343 ± 0.576
4.081AsnLys: 4.081 ± 0.634
4.005AsnLeu: 4.005 ± 0.427
1.058AsnMet: 1.058 ± 0.287
3.174AsnAsn: 3.174 ± 0.587
2.041AsnPro: 2.041 ± 0.433
2.343AsnGln: 2.343 ± 0.549
1.738AsnArg: 1.738 ± 0.362
3.552AsnSer: 3.552 ± 0.557
3.325AsnThr: 3.325 ± 0.463
4.61AsnVal: 4.61 ± 0.614
0.831AsnTrp: 0.831 ± 0.216
2.418AsnTyr: 2.418 ± 0.508
0.0AsnXaa: 0.0 ± 0.0
Pro
3.099ProAla: 3.099 ± 0.526
0.529ProCys: 0.529 ± 0.168
2.041ProAsp: 2.041 ± 0.407
2.192ProGlu: 2.192 ± 0.369
1.36ProPhe: 1.36 ± 0.299
0.0ProGly: 0.0 ± 0.0
0.605ProHis: 0.605 ± 0.181
2.721ProIle: 2.721 ± 0.478
2.645ProLys: 2.645 ± 0.595
3.174ProLeu: 3.174 ± 0.589
1.134ProMet: 1.134 ± 0.251
2.041ProAsn: 2.041 ± 0.447
2.192ProPro: 2.192 ± 0.443
1.889ProGln: 1.889 ± 0.455
1.814ProArg: 1.814 ± 0.373
2.418ProSer: 2.418 ± 0.373
3.703ProThr: 3.703 ± 0.654
2.872ProVal: 2.872 ± 0.448
0.227ProTrp: 0.227 ± 0.142
1.965ProTyr: 1.965 ± 0.441
0.0ProXaa: 0.0 ± 0.0
Gln
3.628GlnAla: 3.628 ± 0.878
0.302GlnCys: 0.302 ± 0.136
1.814GlnAsp: 1.814 ± 0.31
1.587GlnGlu: 1.587 ± 0.308
1.814GlnPhe: 1.814 ± 0.397
2.494GlnGly: 2.494 ± 0.569
0.227GlnHis: 0.227 ± 0.122
1.587GlnIle: 1.587 ± 0.328
2.192GlnLys: 2.192 ± 0.38
3.703GlnLeu: 3.703 ± 0.538
1.965GlnMet: 1.965 ± 0.295
2.57GlnAsn: 2.57 ± 0.586
2.57GlnPro: 2.57 ± 0.534
3.325GlnGln: 3.325 ± 1.071
2.947GlnArg: 2.947 ± 0.679
2.192GlnSer: 2.192 ± 0.37
3.25GlnThr: 3.25 ± 0.501
2.343GlnVal: 2.343 ± 0.442
0.378GlnTrp: 0.378 ± 0.166
1.663GlnTyr: 1.663 ± 0.409
0.0GlnXaa: 0.0 ± 0.0
Arg
3.023ArgAla: 3.023 ± 0.475
0.302ArgCys: 0.302 ± 0.169
2.494ArgAsp: 2.494 ± 0.409
1.285ArgGlu: 1.285 ± 0.317
2.041ArgPhe: 2.041 ± 0.433
1.889ArgGly: 1.889 ± 0.344
0.529ArgHis: 0.529 ± 0.215
2.645ArgIle: 2.645 ± 0.428
2.343ArgLys: 2.343 ± 0.463
4.61ArgLeu: 4.61 ± 0.529
1.587ArgMet: 1.587 ± 0.41
3.703ArgAsn: 3.703 ± 0.513
1.511ArgPro: 1.511 ± 0.329
1.436ArgGln: 1.436 ± 0.391
2.192ArgArg: 2.192 ± 0.448
3.174ArgSer: 3.174 ± 0.477
2.418ArgThr: 2.418 ± 0.512
2.57ArgVal: 2.57 ± 0.442
0.378ArgTrp: 0.378 ± 0.21
1.285ArgTyr: 1.285 ± 0.26
0.0ArgXaa: 0.0 ± 0.0
Ser
5.441SerAla: 5.441 ± 0.664
0.302SerCys: 0.302 ± 0.146
3.703SerAsp: 3.703 ± 0.557
4.534SerGlu: 4.534 ± 0.557
2.721SerPhe: 2.721 ± 0.434
5.366SerGly: 5.366 ± 0.906
0.982SerHis: 0.982 ± 0.276
3.552SerIle: 3.552 ± 0.55
4.081SerLys: 4.081 ± 0.739
4.988SerLeu: 4.988 ± 0.56
1.134SerMet: 1.134 ± 0.353
2.872SerAsn: 2.872 ± 0.46
2.343SerPro: 2.343 ± 0.334
2.343SerGln: 2.343 ± 0.455
1.663SerArg: 1.663 ± 0.364
3.099SerSer: 3.099 ± 0.668
3.099SerThr: 3.099 ± 0.456
3.779SerVal: 3.779 ± 0.471
0.831SerTrp: 0.831 ± 0.252
2.267SerTyr: 2.267 ± 0.554
0.0SerXaa: 0.0 ± 0.0
Thr
6.499ThrAla: 6.499 ± 0.965
0.529ThrCys: 0.529 ± 0.209
3.25ThrAsp: 3.25 ± 0.5
3.779ThrGlu: 3.779 ± 0.472
2.947ThrPhe: 2.947 ± 0.482
5.517ThrGly: 5.517 ± 0.673
0.529ThrHis: 0.529 ± 0.183
4.61ThrIle: 4.61 ± 0.628
3.703ThrLys: 3.703 ± 0.582
5.139ThrLeu: 5.139 ± 0.513
1.058ThrMet: 1.058 ± 0.302
3.854ThrAsn: 3.854 ± 0.542
3.174ThrPro: 3.174 ± 0.46
2.872ThrGln: 2.872 ± 0.583
2.343ThrArg: 2.343 ± 0.511
2.796ThrSer: 2.796 ± 0.512
4.157ThrThr: 4.157 ± 0.765
4.837ThrVal: 4.837 ± 0.681
0.453ThrTrp: 0.453 ± 0.171
2.418ThrTyr: 2.418 ± 0.417
0.0ThrXaa: 0.0 ± 0.0
Val
7.482ValAla: 7.482 ± 1.095
0.982ValCys: 0.982 ± 0.253
4.232ValAsp: 4.232 ± 0.561
3.93ValGlu: 3.93 ± 0.451
2.116ValPhe: 2.116 ± 0.372
4.761ValGly: 4.761 ± 0.681
0.605ValHis: 0.605 ± 0.246
4.157ValIle: 4.157 ± 0.425
5.215ValLys: 5.215 ± 0.56
4.232ValLeu: 4.232 ± 0.455
1.663ValMet: 1.663 ± 0.368
4.61ValAsn: 4.61 ± 0.598
2.041ValPro: 2.041 ± 0.356
2.116ValGln: 2.116 ± 0.413
2.494ValArg: 2.494 ± 0.368
4.232ValSer: 4.232 ± 0.504
5.215ValThr: 5.215 ± 0.517
4.005ValVal: 4.005 ± 0.538
1.36ValTrp: 1.36 ± 0.296
2.418ValTyr: 2.418 ± 0.512
0.0ValXaa: 0.0 ± 0.0
Trp
1.738TrpAla: 1.738 ± 0.403
0.151TrpCys: 0.151 ± 0.094
1.134TrpAsp: 1.134 ± 0.286
0.529TrpGlu: 0.529 ± 0.151
0.453TrpPhe: 0.453 ± 0.18
1.587TrpGly: 1.587 ± 0.44
0.302TrpHis: 0.302 ± 0.117
0.529TrpIle: 0.529 ± 0.211
0.605TrpLys: 0.605 ± 0.191
1.134TrpLeu: 1.134 ± 0.291
0.0TrpMet: 0.0 ± 0.0
0.831TrpAsn: 0.831 ± 0.274
0.0TrpPro: 0.0 ± 0.0
0.68TrpGln: 0.68 ± 0.218
1.058TrpArg: 1.058 ± 0.289
1.058TrpSer: 1.058 ± 0.281
0.982TrpThr: 0.982 ± 0.288
1.209TrpVal: 1.209 ± 0.218
0.378TrpTrp: 0.378 ± 0.158
0.68TrpTyr: 0.68 ± 0.203
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.25TyrAla: 3.25 ± 0.6
0.68TyrCys: 0.68 ± 0.213
2.57TyrAsp: 2.57 ± 0.52
2.041TyrGlu: 2.041 ± 0.379
1.285TyrPhe: 1.285 ± 0.306
2.947TyrGly: 2.947 ± 0.485
0.529TyrHis: 0.529 ± 0.204
2.343TyrIle: 2.343 ± 0.4
2.267TyrLys: 2.267 ± 0.433
2.721TyrLeu: 2.721 ± 0.414
0.982TyrMet: 0.982 ± 0.332
2.343TyrAsn: 2.343 ± 0.493
1.663TyrPro: 1.663 ± 0.457
1.285TyrGln: 1.285 ± 0.269
2.116TyrArg: 2.116 ± 0.424
2.418TyrSer: 2.418 ± 0.567
2.267TyrThr: 2.267 ± 0.459
3.25TyrVal: 3.25 ± 0.42
0.529TyrTrp: 0.529 ± 0.246
1.36TyrTyr: 1.36 ± 0.311
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (13233 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski