Streptomyces phage WheeHeim

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Deltatectivirus; Streptomyces virus WheeHeim

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AXU9|A0A411AXU9_9VIRU Uncharacterized protein OS=Streptomyces phage WheeHeim OX=2500797 GN=14 PE=4 SV=1
MM1 pKa = 7.8ASGKK5 pKa = 7.33MTQAEE10 pKa = 4.34ADD12 pKa = 3.49QQAAEE17 pKa = 4.22FGEE20 pKa = 3.97ILGLVGAAEE29 pKa = 4.42GGGFNMPPMPDD40 pKa = 3.0FVTRR44 pKa = 11.84LMVQQVSEE52 pKa = 4.33EE53 pKa = 4.22FVSVLAQYY61 pKa = 10.64GYY63 pKa = 10.32EE64 pKa = 3.86ISIGLLDD71 pKa = 3.82TGNVAVGLIKK81 pKa = 10.7SSEE84 pKa = 4.2KK85 pKa = 10.46EE86 pKa = 4.0SGPVAGGGYY95 pKa = 10.0VLAEE99 pKa = 4.49FSADD103 pKa = 4.15GIRR106 pKa = 11.84KK107 pKa = 7.71MLEE110 pKa = 3.61MGNN113 pKa = 3.7

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AXW9|A0A411AXW9_9VIRU LysM-like endolysin OS=Streptomyces phage WheeHeim OX=2500797 GN=36 PE=4 SV=1
MM1 pKa = 7.93ASGRR5 pKa = 11.84SMKK8 pKa = 10.35DD9 pKa = 2.57KK10 pKa = 11.37AMASMLKK17 pKa = 10.53DD18 pKa = 3.01KK19 pKa = 10.96GIRR22 pKa = 11.84RR23 pKa = 11.84TTGQCPRR30 pKa = 11.84CHH32 pKa = 6.39KK33 pKa = 10.22FAYY36 pKa = 9.39TIANVNSLLLHH47 pKa = 6.52LNWCQGSRR55 pKa = 11.84AKK57 pKa = 11.03VNN59 pKa = 3.28

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

5556

38

681

154.3

16.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.791 ± 0.677

0.558 ± 0.143

5.634 ± 0.356

5.022 ± 0.532

3.474 ± 0.225

9.413 ± 0.583

1.602 ± 0.21

5.112 ± 0.338

5.256 ± 0.46

6.821 ± 0.434

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.898 ± 0.294

4.95 ± 0.416

5.886 ± 0.542

3.402 ± 0.325

5.112 ± 0.489

6.21 ± 0.352

6.839 ± 0.488

7.073 ± 0.489

1.908 ± 0.206

3.042 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski