Halobacillus salinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halobacillus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3800 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0H3E8|A0A4Z0H3E8_9BACI PTS mannitol transporter subunit IIA OS=Halobacillus salinus OX=192814 GN=E4663_02795 PE=4 SV=1
MM1 pKa = 7.11KK2 pKa = 10.06RR3 pKa = 11.84IVIILVLFLLSGCIAEE19 pKa = 5.25EE20 pKa = 4.24YY21 pKa = 10.68DD22 pKa = 3.58FTPPTVSLTHH32 pKa = 7.04PYY34 pKa = 10.6ADD36 pKa = 3.2QIEE39 pKa = 4.35LEE41 pKa = 4.25EE42 pKa = 5.61ANINWDD48 pKa = 3.27SDD50 pKa = 3.49KK51 pKa = 10.91EE52 pKa = 4.17YY53 pKa = 11.17SKK55 pKa = 10.01EE56 pKa = 4.29TEE58 pKa = 4.64DD59 pKa = 3.52IYY61 pKa = 11.8ALAEE65 pKa = 4.03EE66 pKa = 4.74QEE68 pKa = 4.11QKK70 pKa = 10.86YY71 pKa = 9.68YY72 pKa = 11.26SSGEE76 pKa = 3.78QVRR79 pKa = 11.84IDD81 pKa = 4.48FDD83 pKa = 3.68NQDD86 pKa = 3.73FLIEE90 pKa = 4.67DD91 pKa = 4.14LSAYY95 pKa = 10.03LWQNGSKK102 pKa = 9.44TEE104 pKa = 4.0LQVKK108 pKa = 9.5DD109 pKa = 3.73QEE111 pKa = 4.49FSFPDD116 pKa = 3.35EE117 pKa = 4.19EE118 pKa = 4.81GEE120 pKa = 4.13YY121 pKa = 10.67LIVVDD126 pKa = 5.42LLSDD130 pKa = 3.61NGSAQYY136 pKa = 10.37VGNIILEE143 pKa = 4.33EE144 pKa = 4.0QSS146 pKa = 3.1

Molecular weight: 16.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0GWE0|A0A4Z0GWE0_9BACI SDR family NAD(P)-dependent oxidoreductase OS=Halobacillus salinus OX=192814 GN=E4663_15120 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.02KK14 pKa = 8.47VHH16 pKa = 5.57GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.32VLQRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.93GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3800

0

3800

1081736

26

1526

284.7

32.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.983 ± 0.039

0.597 ± 0.012

5.579 ± 0.037

8.142 ± 0.054

4.518 ± 0.033

7.011 ± 0.045

2.243 ± 0.02

6.853 ± 0.038

5.999 ± 0.033

9.655 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.948 ± 0.022

3.815 ± 0.027

3.695 ± 0.024

4.097 ± 0.027

4.36 ± 0.03

6.122 ± 0.033

5.416 ± 0.038

7.381 ± 0.034

1.118 ± 0.015

3.471 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski