Hucho hucho (huchen)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai;

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 90503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4W5NR54|A0A4W5NR54_9TELE FYVE RhoGEF and PH domain containing 5a OS=Hucho hucho OX=62062 PE=4 SV=1
VV1 pKa = 7.35EE2 pKa = 4.51GQQGDD7 pKa = 4.06VSHH10 pKa = 7.43FDD12 pKa = 3.08HH13 pKa = 7.22LEE15 pKa = 3.89TYY17 pKa = 9.43TRR19 pKa = 11.84DD20 pKa = 3.15ITDD23 pKa = 3.76GVSLTTEE30 pKa = 4.08SFDD33 pKa = 3.9SYY35 pKa = 11.04LDD37 pKa = 3.67EE38 pKa = 4.38VQATIIGDD46 pKa = 3.4KK47 pKa = 11.11GGDD50 pKa = 3.45LLAVLDD56 pKa = 4.56EE57 pKa = 5.32LDD59 pKa = 5.31PDD61 pKa = 4.02TLPNGGVGLGVVMYY75 pKa = 9.16LHH77 pKa = 6.74FLKK80 pKa = 10.95DD81 pKa = 3.26NSLGVGSASEE91 pKa = 4.4GVSLQGSAEE100 pKa = 3.88MGLFVLFIVPSLLTTVVTQLPGCTQTTTLSS130 pKa = 3.37

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4W5LFV9|A0A4W5LFV9_9TELE Ig-like domain-containing protein OS=Hucho hucho OX=62062 PE=4 SV=1
MM1 pKa = 6.29TTRR4 pKa = 11.84PVMTTRR10 pKa = 11.84PVMATGPVMTTGPVMTTGPVMTTGPVMTTGPVMTTGPVITTGPVIPTGPVIPTRR64 pKa = 11.84PVMTTTPVITTRR76 pKa = 11.84PVITTTPVIPSTPVIPTTPVMTARR100 pKa = 11.84PVMTTRR106 pKa = 11.84PVMTTRR112 pKa = 11.84PVMTTRR118 pKa = 11.84PLIPTHH124 pKa = 6.66HH125 pKa = 6.46MGQRR129 pKa = 11.84VHH131 pKa = 6.75

Molecular weight: 13.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49277

41226

90503

47467216

18

8540

524.5

58.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.267 ± 0.007

2.321 ± 0.007

5.19 ± 0.006

6.789 ± 0.011

3.654 ± 0.006

6.546 ± 0.009

2.731 ± 0.005

4.501 ± 0.005

5.614 ± 0.009

9.602 ± 0.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.004

3.893 ± 0.005

5.573 ± 0.01

4.656 ± 0.007

5.607 ± 0.006

8.22 ± 0.01

5.767 ± 0.006

6.474 ± 0.007

1.183 ± 0.003

2.878 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski