Pseudohalocynthiibacter aestuariivivens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudohalocynthiibacter

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6FKU8|A0A6G6FKU8_9RHOB Phosphoenolpyruvate carboxykinase (ATP) OS=Pseudohalocynthiibacter aestuariivivens OX=1591409 GN=pckA PE=3 SV=1
MM1 pKa = 7.04FRR3 pKa = 11.84IAVAMIFASAIPAMAEE19 pKa = 3.57DD20 pKa = 4.41WFALTPEE27 pKa = 4.04HH28 pKa = 5.94STYY31 pKa = 10.56FSGDD35 pKa = 2.83WTGTCQEE42 pKa = 4.3VEE44 pKa = 4.7PYY46 pKa = 10.97ACTAVATIGARR57 pKa = 11.84DD58 pKa = 3.75DD59 pKa = 4.33AGPGQVNLLWQPWGDD74 pKa = 3.22EE75 pKa = 4.62GYY77 pKa = 10.81SFTFFATNAPDD88 pKa = 3.74QITGQQSIMIDD99 pKa = 3.45DD100 pKa = 4.08AVIAVPEE107 pKa = 4.03SDD109 pKa = 5.27YY110 pKa = 11.14FAIWDD115 pKa = 3.86QPDD118 pKa = 3.43GAVRR122 pKa = 11.84MISIGEE128 pKa = 4.25PIVVDD133 pKa = 3.36VLVEE137 pKa = 3.96QMQSGQTLVWDD148 pKa = 4.07WGTGQLTVPLAGFAEE163 pKa = 4.48TFDD166 pKa = 4.28AVTDD170 pKa = 3.76YY171 pKa = 10.99RR172 pKa = 11.84KK173 pKa = 10.17EE174 pKa = 3.87LGQQ177 pKa = 3.71

Molecular weight: 19.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6FK64|A0A6G6FK64_9RHOB Cytochrome c oxidase subunit I OS=Pseudohalocynthiibacter aestuariivivens OX=1591409 GN=ctaD PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4194

0

4194

1331213

20

11572

317.4

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.086 ± 0.053

0.972 ± 0.016

6.247 ± 0.036

5.666 ± 0.032

3.628 ± 0.026

8.66 ± 0.044

2.155 ± 0.022

5.409 ± 0.031

3.097 ± 0.037

9.821 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.883 ± 0.023

2.688 ± 0.025

4.976 ± 0.027

3.261 ± 0.02

6.643 ± 0.04

5.258 ± 0.026

5.668 ± 0.037

7.179 ± 0.031

1.382 ± 0.017

2.32 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski