Cronobacter phage S13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060ANE7|A0A060ANE7_9CAUD Putative sliding clamp DNA polymerase accessory protein OS=Cronobacter phage S13 OX=1327935 GN=S13_246 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.32NLLIAALLFAPSAFAYY18 pKa = 9.32EE19 pKa = 4.29PSQEE23 pKa = 4.05EE24 pKa = 4.12VSFVCKK30 pKa = 10.71GEE32 pKa = 4.0QQCINIVNDD41 pKa = 3.66EE42 pKa = 4.36LSDD45 pKa = 3.44ITVDD49 pKa = 3.12GNLRR53 pKa = 11.84VITPDD58 pKa = 2.91AFRR61 pKa = 11.84EE62 pKa = 4.07QVLDD66 pKa = 3.6YY67 pKa = 11.51CEE69 pKa = 5.47DD70 pKa = 4.07DD71 pKa = 3.52STEE74 pKa = 3.98CQVYY78 pKa = 9.69VKK80 pKa = 10.29MMINTFDD87 pKa = 2.95QAYY90 pKa = 10.22RR91 pKa = 11.84DD92 pKa = 3.97GEE94 pKa = 4.23EE95 pKa = 3.73

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060AH46|A0A060AH46_9CAUD Uncharacterized protein OS=Cronobacter phage S13 OX=1327935 GN=S13_147 PE=4 SV=1
MM1 pKa = 6.72VTIHH5 pKa = 7.25DD6 pKa = 3.7ARR8 pKa = 11.84MILRR12 pKa = 11.84KK13 pKa = 9.66KK14 pKa = 10.62QEE16 pKa = 3.81IKK18 pKa = 10.38DD19 pKa = 3.71AKK21 pKa = 9.39DD22 pKa = 2.89TLRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84LADD32 pKa = 3.3FRR34 pKa = 11.84DD35 pKa = 3.44RR36 pKa = 11.84VKK38 pKa = 11.15NIMRR42 pKa = 11.84CGVSSFRR49 pKa = 11.84FYY51 pKa = 10.76DD52 pKa = 3.51YY53 pKa = 11.8EE54 pKa = 3.75MMQRR58 pKa = 11.84IKK60 pKa = 10.79RR61 pKa = 11.84EE62 pKa = 3.62LDD64 pKa = 2.96ITPSEE69 pKa = 3.92IRR71 pKa = 11.84DD72 pKa = 3.59VLRR75 pKa = 11.84YY76 pKa = 9.68RR77 pKa = 11.84YY78 pKa = 9.41SANRR82 pKa = 11.84NYY84 pKa = 10.03IVRR87 pKa = 11.84NEE89 pKa = 3.71

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

268

0

268

56517

20

1230

210.9

24.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.134 ± 0.155

1.095 ± 0.068

6.425 ± 0.11

6.957 ± 0.141

4.505 ± 0.116

6.172 ± 0.197

1.861 ± 0.072

7.078 ± 0.121

7.789 ± 0.161

7.538 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.088 ± 0.086

5.506 ± 0.115

3.21 ± 0.1

3.489 ± 0.101

4.876 ± 0.098

6.262 ± 0.131

5.743 ± 0.144

6.87 ± 0.11

1.336 ± 0.055

4.066 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski