Salmonella phage 9NA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nonanavirus; Salmonella virus 9NA

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A060D1I7|A0A060D1I7_9CAUD Uncharacterized protein OS=Salmonella phage 9NA OX=1113547 GN=9NA_015 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 9.23ITKK5 pKa = 9.7QEE7 pKa = 3.99LKK9 pKa = 10.99DD10 pKa = 4.7FGACQDD16 pKa = 3.33GFKK19 pKa = 10.75RR20 pKa = 11.84FVNQTNNTDD29 pKa = 3.39EE30 pKa = 4.49PVDD33 pKa = 3.9VVSLIGGCNTVSDD46 pKa = 4.7LLWLAGKK53 pKa = 7.87TLAKK57 pKa = 10.11EE58 pKa = 3.95KK59 pKa = 10.2IVRR62 pKa = 11.84FACDD66 pKa = 3.1CALLNIEE73 pKa = 5.18KK74 pKa = 10.04IRR76 pKa = 11.84PYY78 pKa = 10.44TNDD81 pKa = 2.94YY82 pKa = 10.8NLIVDD87 pKa = 4.77FLNTPTADD95 pKa = 3.3AARR98 pKa = 11.84AADD101 pKa = 3.64AAYY104 pKa = 10.05AAARR108 pKa = 11.84AADD111 pKa = 3.53AAAYY115 pKa = 8.96AAARR119 pKa = 11.84AADD122 pKa = 3.52AAAYY126 pKa = 8.89AAYY129 pKa = 9.93AAYY132 pKa = 9.91AAAADD137 pKa = 3.83AAYY140 pKa = 10.05AAYY143 pKa = 9.92AAYY146 pKa = 9.91AAAADD151 pKa = 3.92AADD154 pKa = 4.28AADD157 pKa = 3.89AAYY160 pKa = 10.65ADD162 pKa = 4.26AADD165 pKa = 4.37AADD168 pKa = 4.13AAAADD173 pKa = 3.96AAYY176 pKa = 10.03AAADD180 pKa = 3.55AAYY183 pKa = 10.03AAAADD188 pKa = 3.55AAKK191 pKa = 10.34INKK194 pKa = 9.35LLVKK198 pKa = 10.29LFSEE202 pKa = 4.76

Molecular weight:
20.65 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A060D562|A0A060D562_9CAUD Large terminase subunit OS=Salmonella phage 9NA OX=1113547 GN=9NA_011 PE=4 SV=1
MM1 pKa = 7.62WNICHH6 pKa = 7.05KK7 pKa = 10.16SGRR10 pKa = 11.84VLFTTGNKK18 pKa = 6.69QTALNRR24 pKa = 11.84AKK26 pKa = 10.82YY27 pKa = 8.09GWRR30 pKa = 11.84VEE32 pKa = 4.15KK33 pKa = 10.88VNKK36 pKa = 9.52EE37 pKa = 3.83FQINEE42 pKa = 3.31IWLSPRR48 pKa = 11.84GYY50 pKa = 9.36FYY52 pKa = 11.26KK53 pKa = 10.9VIDD56 pKa = 4.31IEE58 pKa = 4.3NKK60 pKa = 9.68HH61 pKa = 6.86AILKK65 pKa = 9.33MGTHH69 pKa = 6.24GLGRR73 pKa = 11.84KK74 pKa = 8.65IKK76 pKa = 9.95RR77 pKa = 11.84RR78 pKa = 11.84KK79 pKa = 8.88DD80 pKa = 3.25TTKK83 pKa = 10.27NWQLHH88 pKa = 4.38TKK90 pKa = 9.88VYY92 pKa = 11.22NNGG95 pKa = 3.14

Molecular weight:
11.3 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

16269

40

971

193.7

21.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.882 ± 0.639

1.211 ± 0.148

6.233 ± 0.234

5.655 ± 0.201

3.823 ± 0.231

7.149 ± 0.264

1.813 ± 0.172

5.839 ± 0.215

5.723 ± 0.312

7.702 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.148

5.003 ± 0.249

4.407 ± 0.32

4.29 ± 0.583

5.249 ± 0.262

5.52 ± 0.225

6.485 ± 0.318

7.019 ± 0.292

1.678 ± 0.126

3.713 ± 0.194

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski